The object of this research is to produce human anti-MUC1 genetic engineering Single-chain Fv Fragment antibody, determine the location in which soluble antibody is expressed and screen the highest expressing colonies.
本实验的目的是制备抗人MUC1基因工程单链抗体，确定可溶性抗体表达部位并筛选表达量高的菌落。
AIM: To screen candidate hepatocyte binding proteins interacting with the surface antigen protein of hepatitis B virus. METHODS: Polymerase chain reaction was used to amplify SHBs gene.
目的：自肝细胞cDNA文库中筛选与HBV主蛋白相互作用的蛋白基因，探讨主蛋白的生物学功能。
My company has the most advanced weaving equipment, strong technical force, scientific process, quality inspection system, using high quality raw materials, strictly the quality pass, complete product specifications, quality excellent, the main products are: stainless steel wire mesh, welded wire mesh, US-grid screens, mine screen mesh, square wire mesh, chain link fence, copper wire mesh, hexagonal wire mesh, fencing wire mesh, barbed wire, razor blades gill nets, wire meshes, steel plate net, stainless steel wire, stainless steel annealed wire, galvanized wire, etc., widely used in petroleum, chemical, building, textile, medicine, aviation, aerospace, building, highway guard rails and other industries.
我公司拥有最先进的织机设备，技术力量雄厚，工艺流程科学，质量检测体系完善，采用优质的原材料，严把质量关，产品规格齐全，质量精良，主要产品有：不锈钢网、电焊网、美格网窗纱、矿筛网、方眼网、勾花网、铜丝网、六角网、护栏网、刺绳、刀片刺网、烧烤网、钢板网、不锈钢丝、不锈钢退火丝、镀锌丝等，广泛用于石油、化工、建筑、纺织、医药、航空、航天、建筑、公路护栏等行业。
The pacemaker in Chinese self-propelled turner industry Baoding Limin Machine manufactured goldbug self-propelled turner, fermented manure turner, mud turner, garbage disposal equipment, dejecta ferment turner, tiller, organic fertilizer equipment, mushroom feeding medium turner, cow dropping turner, roll granulating machine, classification revolving screen, chain disintegrator etc.
中国自行式翻堆机行业的领跑者-保定利民机械制造金甲虫自走式翻堆机、堆肥发酵翻堆机、污泥翻堆机、垃圾处理设备、蓄勤粪便发酵翻堆机、翻抛机、翻拌机、有机肥设备、蘑菇培养料翻堆机、牛粪翻堆机、对辊制粒机、分级回转筛、链式粉碎机等。
Fasting blood glucose levels in 236 patients with type 2 diabetes mellitus and 252 healthy subjects were determined respectively. Polymerase chain reaction restriction fragment length polymorphism analysis was used first to screen point mutations of mtDNA at positions of 3243, 3316, 3394, and 3593; DNA sequencing was adopted to confirm the mutations followed; all the mutations were analyzed by PRIMER and NCBI BLAST softwares at last.
对236例海南地区2型糖尿病患者和252例健康对照者进行空腹血糖测定并采用聚合酶链式反应－限制性核酸内切酶片段长度多态性的分析方法进行mtDNA的3243、3316、3394、和3593共4个位点进行突变筛选，并采用DNA测序方法确证。
The much home hospital that divided network of broadcast of numerous medium medicine to enclothe 31 cities of Chinese and chain drugstore, operation is worn screen of liquid crystal of about 2461 advertisement.
分众传媒医药联播网覆盖了中国31个城市的多家医院和连锁药店，运营着约2461块广告液晶屏。
Divide numerous medium (Nasidake: FMCN) announced recently, limited company of medium of yellow health of company general Xiang Yan invests ready money of 5 million dollar, the network of Medical Protection broadcast that hospital of 31 towns of domestic and drug chain store still will enclothe below the banner at the same time (installed 2461 liquid crystal to display screen about) droit makes over medium of phlogistic Huang Jiankang, in order to acquire this company the share of 20%.
分众传媒近日公布，公司将向炎黄健康传媒有限公司投资500万美元现金，同时还将旗下覆盖国内31座城市医院和药品连锁店的医疗保健联播网(大约安装了2461块液晶显示屏)所有权转让给炎黄健康传媒，以获得该公司20%的股份。
In China, Hubei, Hunan and Jiangxi provinces of the screen Fau Shan Ha marina, beautiful Youjuan River rising gap in China's modernization Abrasives industry --- a shining pearl in Hubei Yuli Abrasive Belt Group Co., Ltd. after more than two years of trials and hardships, Yuli Belt Group engaged in a hands workshops, developed to an area of 750 acres, with 350 million of assets, more than 2, 400 employees, Tongcheng Wuhan Metropolitan Area has become an extension of the economic chain.
在中国鄂、湘、赣三省壤的幕阜山下，美丽的隽河畔，冉冉升起拉我国现代化附磨具行业中的一颗璀璨明珠---湖北玉立砂带集团股份有限公司经过二十多年的风雨历程，玉立砂带集团从事一个手工作坊，发展到今天占地750亩，拥有3.5亿资产、员工2400余人，通城成为武汉都市圈的经济延伸链。
By 5:00PM the local production team and the local union had successfully removed the LED screen, conveyor belts, and chain motors from the main stage, completed the construction of the main stage, begin the construction of the 2nd stage, finished all lighting installations, finished all sound installations and began working on the remainder of the video screens and stage.
到下午的5：00，当地制作方和团体成功移除了LED屏，传送带和提升机，完成了主体舞台的搭建，开始下一步舞台搭建，完成了所有的灯光设备，音响设备并且开始影像屏幕的工作。
During this time Staples Center had advised STAR M to remove the non-approved LED Screen, chain lift motors, and conveyor belts from the stage and commence production because of a constrained time schedule.
在此期间，Staples Center已经建议 STAR M从舞台上移除这些未经许可的LED屏，锁链提升传动机和输送带。
His solution would see Richina's corporate customers bolster management of their supply chain. Big brands such as Nike and Armani must screen suppliers carefully, says Ma. Otherwise, their environmental commitments are just a sop to trusting consumers.
对于如何解决这个问题，马军提出，必须加强富国客户企业对供应链的管理，像Nike、Giorgio Armani这些大型品牌，都有各自对环境的承诺，所以一定要有严格的甄别管理，不然对环境的承诺只是忽悠信任你的消费者。
Plastic gift: Professional development and production of various types of soft gifts, such as Block Mobile Mobile Phone Charm Mobile cell phone non-slip pad frame cartoon frame lock key chain notebook luggage tag wrist band silicone hand Central mug car front anti-skid pad shockproof mat cup cushions bar pad mats mouse pad Mobile Sassafras U disk shell U disk sets brooch bracelet Watch band Fridge paste hairpins bookmarks book folder pen cartoon cap of a pen, such as soft/Epoxy/PVC micro-injection products, the other for all types of clothing, sports shoes, handbags, toys and gifts such as plants to provide plastic chapters, screen printing, printed standard, self-adhesive stickers, woven Tianbiao, Paper card, tag and other accessories.
塑胶礼品：专业开发生产各类软胶礼品，如手机座手机吊饰手机来电闪手机防滑垫相框卡通相框锁匙扣笔记本行李牌手腕带硅胶手环马克杯汽车前台防滑垫防震垫杯垫桌垫酒吧垫地垫鼠标垫手机檫U盘外壳U盘套胸针手镯手表带冰箱贴发夹书签书夹笔筒卡通笔套等软胶/滴胶/PVC微量射出制品，另外为各类服装、运动鞋、手袋、玩具等厂及礼品提供胶章、丝印、印标、不干胶贴纸、织标、皮牌、吊牌等辅料。
A live screen is one of the most captivating sights on Earth, right up there with nude co-ed volleyball or a chain collision on an expressway.
有即时波动的电脑屏幕是这个地球上最迷人的地方，和裸体男女排球，公路连环撞车一样迷人。
To optimize and screen the most suitable target gene length, concentration and printing solution in cDNA microarray, housekeeping genes, such as beta actin and GAPDH, were selected as targets and hepatitis B virus gene as negative control. The RT-PCR primers that spanned at least one intron and whose products were at between 189 bp and 1078 bp were designed with primer premier 5.0, so did the hepatitis B virus gene PCR primer. After polymerase chain reaction, the products were purified with ethanol and dissolved in 3×SSC, 50% DMSO and 0.5mol/L carbonate buffer（pH=9.0）respectively.
为了优化筛检cDNA微阵列中靶基因的最适长度、浓度及点样溶液的种类，设计持家基因beta actin和GAPDH RT-PCR 3对引物，产物长度在189～1 078 bp之间，以乙肝病毒DNA片段为阴性对照，扩增纯化后分别溶于3×SSC、50%DMSO及0.5mol/L碳酸盐缓冲液（pH=9.0）中，调整浓度分别为0.5μg/μL、1.0μg/μL和1.5μg/μL，比较上述不同条件的杂交结果。
Fengtai mainly supplies various wire and wire mesh products at full sizes, mainly includes Stainless Steel Wire Mesh insect screen, Stainless Steel Wire Mesh, Brass Wire Mesh, Welded Wire Mesh, stainless steel crimped Wire Mesh, galvanzied iron wire, annealed iwre, Crimped Wire Mesh, Chain Link Fence, Squre Wire Mesh, Perfoared Metal Sheet, Barbed Wire, Black Wire Cloth, Barbecue Grill Netting, Rib Lath, Iron Wire, Galvanized Wire, etc.
我公司科研人员经过多年的研究创新，产品不断推陈出新，形成以不锈钢窗纱，不锈钢网，磷铜网，电焊网，不锈钢轧花网，矿筛网，汽液过滤网，席型网，各种金属线材产品为主的3大类，80多个品种，产品主要用于石油、化工、建筑、纺织、医药、航空、航天、高速公路铁路、园林防护、水产、养殖、冶金机械等各个领域。
Methods Ninety-eight strains of non-repeat clinical isolated E. cloacae were collected from the hospital from August 2007 to July 2008, cefoxitin disk diffusion method was used to screen AmpC enzyme of enzyme of E. cloacae was confirmed by enzyme extract three-dimensional high yield teats, the AmpC enzyme gene was detected with polymerase chain reaction.
收集该院2007年8月－2008年7月临床分离的非重复阴沟肠杆菌98株，采用头孢西丁纸片扩散法进行AmpC酶初筛，酶提取物三维试验确证阴沟肠杆菌高产AmpC酶，聚合酶链反应检测AmpC酶基因。
Applies CPU microprocessor and PID control technique, large screen Chinese display, and menu operation Heating with double helix silicon carbide rod; temperature measured with platinum-rhodium thermocouple Up to 4 samples can be fused simultaneously (maximum 6 samples if required by user) Up to 10 customized heating programs can be preset; within the programs, the heating process, the temperature increase speed, the final temperature, the shaking time, shaking amplitude, and shaking speed of the crucible stool, the cooling time, whether to use fans, the air flow, and so on, all can be preset. Automatic control in all the process except for the filling of the fusion sample, enabling the sameness and homogeneity of the sample; all samples are uniformly the same except for the differences in composition The bracket of the crucible and the mould are made of high temperature alloy, which guarantees no chemical reaction in high temperature Chain wheel lever structure is used in the opening device of the cover of the fusion room, trouble-free for user operation With minimum start temperature alarm and power switch-off protection when the cover of the fusion room is open Automatically switching off power and setting alarm for overcurrent and overheat; independent temperature measurement thermocouples are used for overheat protection and heating control; together with the heating control circuit, they can form treble protection of the system and can make the power switch-off protection for overheat more reliable.
主要特点：◆采用CPU微机及PID控制技术，大屏幕中文显示，菜单式操作；◆双螺旋硅碳棒加热，铂铑热电偶测温；◆最多可同时熔融4个样品(可根据用户要求增加到6个)；◆可预先自由设定最多达10个加热制样程序，其加热过程，升温速度，最终温度，坩埚架摇摆时间，幅度，速度，冷却时间，是否用风扇，风量大小等均可预先设定；◆除熔融样注入成型模具采用手动外，其他均自动控制，保证了样品的一致性和均匀性，真正做到不同样品仅是其成分不同而没有其他不同；◆坩埚及成型模具的支架采用耐高温合金钢制成，保证在高温状态下无任何化学反应；◆熔样室盖打开装置采用链轮杠杆结构，操作轻松自如；◆具有最低启动温度报警，打开熔样室断电保护；◆具有过流，过温断电保护并报警，并且过温断电保护采用与加热制样独立的热电偶测温，与加热控制电路一起组成三重保护，使过温断电保护更可靠。
This company mainly produces stainless steel wire mesh, welded wire mesh, wire mesh, Crimped Wire Mesh, hexagonal wire mesh, black wire cloth, steel nets, round hole net, chain link fence, fencing wire mesh, copper wire cloth, wire meshes, mining screen mesh, the carrier network belt, such as galvanized wire mesh products.
本公司主要生产不锈钢网、电焊网、窗纱、轧花网、六角网、黑丝布、钢板网、圆孔网、勾花网、护栏网、铜丝布、烧烤网、矿筛网、输送网带、镀锌丝等丝网产品。
The main products are: aluminum net, steel net, stainless steel wire mesh, black wire cloth, galvanized steel wire, iron aluminum net Luo, mining screen mesh, paper net, gas-liquid to worry too much about networks, conveyor belt net, hexagonal wire mesh, welded wire mesh, Chain Link Fence, audio network, nylon net, ethylene network, network brass, copper network, pre-curved flat screen flat-topped, plastic-coating Garden Wai block and sample processing a variety of special-shaped network is widely used in petroleum, chemical, rubber, automotive, medical, aviation, aerospace and other industries to worry too much about, screening, shielding and so on.
主要产品有：铝板网、钢板网、不锈钢网、黑丝布、镀锌钢丝网、铁硌铝网、矿筛网、造纸网、气液过虑网、输送带网、六角网、电焊网、勾花网、音响网、锦纶网、乙烯网、黄铜网、紫铜网、平顶预弯扁丝网、涂塑花园围拦网及来样加工各种异形网等，广泛应用于石油、化工、橡胶、汽车、医药、航空、航天等行业过虑、筛分、屏蔽等。
We are one of the largest exporters and manufactures of wire mesh in China, specialized in selling all kinds of metal wire mesh product, including welded wire mesh, stainless steel wire mesh, expanded metal mesh, plastic plain netting, window screen, crimped wire mesh, chain link fence, perforated metal mesh, wire mesh fences, nylon netting, hexagonal wire mesh, conveyer belt etc., also offering metal wire products, such as barbed wire, galvanized wire, PVC coated wire, copper wire etc.
我们是最大的出口商之一，在中国网丝生产，销售金属丝网产品，包括电焊网，不锈钢丝网，钢板网，塑料平网，窗纱，轧花网各类专门，勾花网，冲孔网，护栏网，尼龙网，六角网，输送带等，还提供诸如带刺铁丝，镀锌铁丝金属丝产品，PVC涂塑丝，铜丝等，广泛应用于石油，化工，食品，医药，建筑，家禽和牲畜等，我们将每年销售超过五〇〇〇〇〇〇〇平方米丝网。