MIXED CULTURE has selected high-density cotton fabric, texture soft comfort, style design to Chinese and Western cultures for the details, global fashion forefront of popular classics of a few meters Charm and good hand-painted cultural integration Together to form the unique style of fashion.
MIXED CULTURE始终选用高密度的纯棉布料，质感柔和舒适；款式设计以中西文化为底蕴，将全球时装流行前沿的风韵和经典的几米手绘文化很好的融合在一起，形成风格独具的时尚品牌。
On the basis of investigating the resource and utilization of Iris plants in Nanjing, Hangzhou and Wuxi, the paper selected five plants: Iris pseudacorus、Iris sanguinea、Iris kaempferi、Iris japonica、Iris tectorum to find their tissue culture technology and shade tolerance with lots experiments. The results were summarized as follows:In the culture of Iris sanguined, The shooting tip in the spring and the medium supplemented with 6-BA(1mg/L)+NAA(0.2mg/L) testified the best grouping.
本文在对南京、杭州、无锡等地鸢尾属植物及其园林应用实地调查的基础上，以黄菖蒲、溪荪、花菖蒲、蝴蝶花、鸢尾等五个种为实验材料，从其部分种的组织培养技术和耐荫性两方面入手，通过大量的实验并对所得数据进行统计分析，得出以下结论：溪荪的组织培养中，以春季萌发的茎尖为外植体，以MS培养基附加低浓度的6-BA(1mg／L)与NAA(0.2mg/L)为最佳的诱导分化的组合。
From strains selected, conditions of submerged culture to extracting, purifying, basic physicochemical characters and structural properties of glycoconjugate. Many aspects were studied by the numbers in the article.
论文从菌株的选取、深层培养工艺条件的确定，尤其在灰树花菌丝体多糖的提取、纯化、基本特性和结构表征等多方面进行了较为全面和系统的研究。
The selected positive clones were expanded and the level of IgG in the culture supernatant was measured.
筛选出的各阳性细胞克隆经扩大培养及ELISA定量检测其上清人IgG含蛰最高为4.05ng／ml。
The test cells: V79 cells were bought from Biochemistry and Cell Biology of CAS and stored in fluid nitrogen after being selected by 1% HAT culture medium for 7 days.
细胞 V79细胞购于中国科学院生物化学与细胞生物学研究所，用1％次黄嘌呤-氨基喋呤-胸腺嘧啶培养基处理7d，分成若干小瓶，液氮冻存备用。
Oases in the Tarim Basin are selected as a target area in which the formation of eco-ethics, its transmission and transition are probed through case study and field work, revealing aspects of the interaction between human culture and the changes of oasis.
第三章以从田野调查中获得的第一手材料为主要内容结合前人收集的有关资料，在宏观梳理与微观实证、面与点相结合的基础上，围绕本研究的主旨，解析绿洲生活的主要层面，使内隐在林林总总民俗之中与人们习而不察的文化事象之中的意一义、文化深层结构凸现出来，从而显示出蕴含于民俗文化模式中的规范人与自然关系的理念。第四章传承与演变以民俗学与生态伦理学理论为主导，探讨维吾尔生态伦理的保存、继承与演变问题，分析显示其传统与擅变与绿洲环境变迁之间的互动关系。
Every microorganism experiment was selected as one of three dilute degrees and repeater three times, and then adopted to lay soil solution on the culture medium, which were put on biochemistry foster cases at 28℃ and counted.
土壤微生物区系采用稀释平板涂抹法分析；接种后置28℃生化培养箱内培养；分取其合适的稀释度来计数。
This paper described the infection process of vesicular arbuscular mycorrhizal fungus Gigaspora margarita in transformed Ri T-DNA carrot roots under axenic condition Agrobacterium rhizogenes was used to introduce Ri T-DNA into the root cells of carrot and then the adventitious roots of carrot were used as host Four routine methods of surface sterilization were compared and one was selected for obtaining maximum amount of pure mycorrhizal fungal spores After two months of dual culture, the infection of transformed Ri T-DNA carrot roots by VA mycorrhizal fungus was observed and mature spores were obtained Under suitable conditions, the harvested spores were capable of germinating and subsequently infecting the host roots immediately
在无菌条件下观察珠状巨孢囊霉对转移RiT-DNA胡萝卜根器官的侵染过程。采用发根土壤杆菌诱导出细胞中整合有RiT-DNA胡萝卜的不定根作宿主，比较了孢子的4种表面消毒方法，经过2～3个月的双重培养，看到VA菌根真菌对根器官的侵染，并获得成熟孢子。形成的孢子无须休眠，只要提供发芽条件，就能立即发芽，接种到根的周围又能侵染。文中对侵染的全过程作了较详细的描述，并对此项技术在菌根研究中的意义进行了讨论关键词 VA菌根，转移Ri T-DNA胡萝卜根，双重培养
We selected 400μM cobalt chloride density and culture for 24h as subsequent experimental condition.
结果显示400μM氯化钴浓度和培养24h是最佳实验条件，并选之为后续实验的浓度和时间。
Copper contaminated soil was selected, Brassica juncea and Elsholtzia splendens plants were used, then simulation experiment, hydroponic culture, column leaching experiment and pot experiment were conducted to study (1) effects of organic chelants on the Cu specition in soil solution and soil soild phase; (2)Effect and mechanism of organic chelants on plant growth and Cu uptake by plant; (3) nutrient addition on plant growth and Cu accumulation; (4) limitation of chelant in soil; (5) Desolving on nutrient and heavy metals in soil and their environmental risk by chelant application and (6) effects of degradable organic chelant and microbial materials on plant growth and phytoremediation efficiency of Cu contaminated soil.
选择铜污染土壤，利用印度芥菜和海州香薷植物，通过室内模拟试验、水培试验、土柱淋溶试验、温室盆栽试验，开展了以下六方面的工作：（1）有机络合剂对土壤铜固-液相形态、浓度变化的影响；（2）有机络合调控对对植物生长与铜吸收的影响及其机理；（3）养分调控对植物生长和铜积累的影响；（4）有机调控剂的作用时限；（5）有机物对土壤养分、重金属的络合溶解和淋溶作用与环境风险；（6）可降解络合剂及微生物菌剂对海州香薷修复污染土壤的效应。
In this study, 130 strains of actinomycetes were isolated from 80 soil samples which collected from various areas in the south-wei dry plateau. One genetically stable actinomycetes strain S-159-05was selected after comparison on inhibition activity of pathogenic fungi by means of selected in solied culture and fermental filtrate.
本试验首次从陕西渭北旱原的不同类型的土壤中采集了80个土样，并从中分离到了130株放线菌，通过皿内平板初筛、液体发酵复筛和单孢分离，得到了一株对多种植物病原真菌颉颃性较好且遗传稳定的新菌株
This paper selected the traditional farm implement images in TRADITIONAL FARM IMPLEMENTS COLLECTED BY THE AGRICULTURAL MUSEUM OF CHINA and IMAGE SET OF CHINESE FARM IMPLEMENTS, objects collected by The Chinese Agricultural Culture Museum, Luzhi Farm Implement Museum and Suoga Ecological Museum as resources of collections, accomplished data acquisition of about 2100 traditional farm implement collections in a mean which combined direct digitization and indirect digitization.
本研究利用直接数值化与间接数值化的手段，以《中国农业博物馆馆藏中国传统农具》与《中华农器图谱》两本图册所载传统农具实物图像，以及中华农业文明博物馆、角直农具馆及梭戛生态博物馆三个实体博物馆的馆藏传统农具为藏品来源建立数字馆藏，实现了约2100件农具藏品的数字化信息采集工作。
Oxysporum isolate F-H.6.5-030318-J2 from cucumber and F-P.5.0-030710 from peanut were selected. The standard cultural conditions were set as follow: the culture media were PSA, the liquid volumes for culturing were100ml/250/ml, inoculation amount of fungi were 3×6 mm fungual patches cutted from the solid cultural media where the fungus had growed for 7 days, the temperature was 25±1℃, and the rotate speed was 110r/min. The cultures of the fungi were conducted for 10 days. The colors, OD values and pH values of the fermentation liquid were observed, as well as the dry weights of mechlial of the fungi were detected at each day.
供试菌株为黄瓜尖孢镰刀菌F-H.6.5-030318-J2和花生尖孢镰刀菌F-P.5.0-030710，培养基采用PSA培养基，装液量为100ml/250ml，接菌量从培养7d的平板上打取3个6mm的菌片，培养温度为25±1℃，摇床转速为110r/min，培养10d，观察测定发酵液颜色、OD值、pH值、菌丝干重变化，并利用聚类分析方法划分生长阶段。
METHODS Plasmid vector pcDNA3.1-CB2 that contains a cDNA fragment coding for human CB2 was transfected into CHO cells by a Lipofectamine based method, and transfected CHO cells were selected in culture medium containing G418. Positive cell clones were measured by RT-PCR. The DNA fragment was sequenced. The activity of CB2 was analyzed by radioligand receptor binding assays.
通过脂质体介导的方法将构建的表达载体pcDNA3.1-CB2转染入CHO-K1细胞中，然后用含G418的选择性培养液进行筛选，挑取耐药克隆；培养并收集耐药克隆细胞，用RT-PCR方法做进一步筛选；序列测定鉴定整合基因的序列；筛选的阳性克隆用放射性配体－受体结合实验进行进一步的鉴定和受体活性分析。
Sixty two lines from two DH populations derived from microspore culture of two interspecies hybridization combinations of B. napus that include Qingyou14×Sida and Beishanfan×46, among which, lines 1~31 and lines 32~66 were from combination Qingyou 14×Sida and Beishanfan×46 respectively, were selected randomly for genetic diversity analysis by simple sequence repeat with thirty polymorphic primer pairs. The 75.2% and 74.1% of total amplified fragments, for eighty-two and eighty polymorphic fragments whose length ranging from 120 to 900bp, were detected among lines131 and lines 3266 respectively.
由甘蓝型油菜杂交组合青油14号×四达和北山繁×46号的F1植株通过小孢子培养所获得的DH群体中，各随机选择31个DH系，分别编号为：1~31，32~62，采用SSR标记对其进行遗传多样性分析。30对SSR引物分别扩增出82、80个多态性片段，其中多态性位点各占总扩增位点的75.2%和74.1%，片段大小介于120~900 bp间。
Based on the above investigation, n-Undecane was selected as a representative carbon source and the culture conditions of C.
在此基础上，选择正十一烷为代表碳源，考察了南极假丝酵母的基本发酵条件，得到其适宜的摇瓶发酵条件为
All selected tissues from different positions of the same Lactarius deliciosus basidiocarp can germinate mycelium on PDA. It showed that different position tissues could be used in isolation culture.
在PDA培养基上，Lactarius deliciosus子实体不同部位的组织均能萌发长出正常菌丝，说明不同部位的组织都可用于分离培养。
In the scale of household land possession and operation condition, the farmers universally selected part-time farming, besides economical considering, a prior cause consisted in the need of household population reproduction and the successive kin ethics, addicting baby girl was namely a type of economy select establishing on culture rite.
近代江南农民在狭小的家庭土地占有和经营规模条件下，普遍地选择兼业，除经济的考虑之外，更重要的原因在于家庭人口的再生产需要和宗祧继承的家族****观念，溺女婴就是一个典型地建立在文化习俗基础上的经济选择。
Duodenal drainage was conducted using a gastroscope. Biliary bacteria were cultured and tested for drug susceptibility. Appropriate antibiotics were selected according to the results of the drug susceptibility test. Result For the patients with chronic severe hepatitis, the positive rate of biliary bacterial culture was 93.30%. 55.93% of the isolated bacteria were Gram-negative bacilli, and 27.97% were Gram-positive coccobacteria.
本文对2002年1月至2006年12 月在我院住院的重型肝炎307例中的105例行十二指肠引流，并将引流出来的胆汁进行细菌培养及药敏试验，并根据药敏选择抗生素进行治疗，提高了重型肝炎的疗效。1 材料与方法1.1 诊断标准作者简介：范慧敏，女，副主任医师，主要从事肝病治疗，特别是慢性乙型、丙型肝炎的抗病毒治疗及重型肝炎的抗感染治疗。
Methods: The rabbit thighbone was selected to flush medullary cavity of bones, for the culture of the cell to paste the wall till the fourth generation, and bone derivative DMEM culture medium was added. The result was compared with that of the control group; the marrow matrix stem cell multiplication ability in the two groups was examined by MTT, and dyed with the osteoblast the calcium tubercle sodium alizarinsulfonate and I collagen immunity.
取兔股骨，冲洗骨髓腔，进行细胞的贴壁体外培养，培养至第四代加入含成骨诱导剂的DMEM培养基与空白组为对照组；噻唑蓝检测两组骨髓基质干细胞的增殖能力，进行成骨细胞的钙结节茜素红染色与I型胶原的免疫组化染色。