Endostatin was amplified from Pshuttle-Endostatin plasmid with PCR and subcloned into the pAdTrack-CMV shuttle vector after sequencing and identification. The resultant plasmid was co-transduced into E. coli BJ 5183 cells with pAdEasy-1 plasmid for homologous recombination. The recombinant plasmid was detected by restriction analysis and DNA sequence analysis. The linearized recombinant plasmid was transfected into AAV 293 cells with LipofectAMINE2000. The recombinant adenovirus was detected by GFP, PCR and restriction analysis. The virus was used to infect ECV304, and the expression of endostatin in vitro was detected by the techniques of RT-PCR and Western blot.
以Pshuttle-Endostatin质粒为模板，应用特异引物，通过聚合酶链反应扩增纯化得到Endostatin基因片断，经测序鉴定后，酶切插入穿梭质粒pAdTrack-CMV中，再与骨架质粒AdEasy-1在大肠杆菌BJ5183中进行同源重组，重组质粒经筛选、提取、纯化后，经酶切及测序鉴定正确，再大量扩增为腺病毒载体，线性化后利用脂质体2000转染AAV293细胞包装并扩增，得到的病毒液纯化扩增后，感染人脐静脉内皮细胞，反转录-聚合酶链反应检测感染细胞中目的基因的表达，蛋白免疫印迹检测感染细胞中蛋白的表达。
This paper corrected the calculation equation of soil porosity by means of the hydrous states of montmorillonite interlayer water, then the mathematic expression was set up to calculate the amount of secondary land subsidence resultant from montmorillonite interlayer dehydration. The model was applied to the Shitangwan land subsidence area, Wuxi City.
用蒙脱石层间水化状态修正了土壤孔隙率的计算方程，以此建立蒙脱石层间脱水作用所产生的次要地面沉降量的数学表达式，并对无锡石塘湾地面沉降区进行了模拟计算。
Effects of the resultant PEP4 strain on heterologous protein expression were then measured by practical expression compared with the wild strain.
液泡蛋白酶首先以无活性的酶原形式合成，然后在液泡中经蛋白质水解作用去除前肽变为活性肽。
The resultant constructs were transfected into NIH 3T3 cells using lipofectamine and PLUS Reagent. The cells were then treated with 5-fluorouracil (5-FU) and expression of the reporter gene in the cell lysates was assayed using Dual-Luciferase Reporter Assay System.
用脂质体转染法将phP53-luc转染入NIH 3T3细胞并分别用5-氟尿嘧啶(5-Fu)处理，最后裂解细胞用Dual-Luciferase Reporter Assay System检测荧光素酶报告基因表达。
And also got a new constructive root-bound algorithm based on resultant for algebraic expression;
并用结式的基本理论给出了求代数数根界的一个有效算法。
In this paper, we have given a new effective algorithm for root bound of the algebraic expression based on the resultant of the algebraic polynomial, also, with MAPLE's help, we have realized this new algorithm in our microcomputer.
得出了代数数根界的一个基于结式的有效实现算法，并且已用计算机代数系统MAPLE在微机上实现。
In addition, we also drawed resultant fatigue life curve and expression which were characterized by total strain and pseudo & stress for these materials, discussed the relationship between initial cracking life versus failure life of the tests, and proposed empirical formula.
文中还给出了分别用应变和虚拟应力表示的三种材料的综合疲劳寿命曲线和表达式。此外，还对裂纹起始寿命和失效寿命间的关系进行了探讨，并给出了经验关系式。
Creates a resultant column that is a character expression.
产生一个字符表达式组成的新的列。
The resultant expression of critical pressure is similar to the well - known Mises formula of cylinder shell.
所得到的临界压力表达式和熟知的圆柱壳Mises公式相似。
To further investigate the effect of H-NS on APP biofilm formation, the 408 bp complete coding sequence of hns gene was amplified by PCR from the genomic DNA of APP serotype 1 strain 4074 and cloned into the prokaryotic expression vector pET-28c. The resultant recombinant plasmid pET-hns was transformed into Escherichia coli BL21 (DE3). A 19KDa of recombinant protein was obtained by IPTG induction, and purified using the Ni-NTA agarose columns.
为了进一步研究H-NS蛋白对APP生物被膜形成的影响，本文以APP血清1型4074株基因组DNA为模板，PCR扩增了408bp的hns基因编码区，克隆到原核表达载体pET-28c中获得重组质粒pET-hns，转化大肠杆菌BL21(DE3)，经IPTG诱导表达和组氨酸亲和层析柱纯化获得大小约19KDa的重组蛋白rH-NS。
Firstly, oscillation between events of imperial and of local interest, the anticipated diamond jubilee of Queen Victoria (born 1820, acceded 1837) and the posticipated opening of the new municipal fish market: secondly, apprehension of opposition from extreme circles on the questions of the respective visits of Their Royal Highnesses the duke and duchess of York and of His Majesty King Brian Boru: thirdly, a conflict between professional etiquette and professional emulation concerning the recent erections of the Grand Lyric Hall on Burgh Quay and the Theatre Royal in Hawkins street: fourthly, distraction resultant from compassion for Nelly Bouverist's non-intellectual, non-political, non-topical expression of countenance and concupiscence caused by Nelly Bouverist's revelations of white articles of non-intellectual, non-political, non-topical underclothing while she was in the articles: fifthly, the difficulties of the selection of appropriate music and humorous allusions from Everybody's Book of Jokes (1000 pages and a laugh in every one): sixthly, the rhymes, homophonous and cacophonous, associated with the names of the new lord mayor, Daniel Tallon, the new high sheriff, Thomas Pile and the new solicitorgeneral, Dunbar Plunket Barton.
123首先，有关皇室与当地的两档子事，歌中究竟写哪一桩，令人难以做出抉择。要么是提前描写维多利亚女王（一八二0 年出生，一八三七年即位）的六十周年大庆；要么是将新修建的市营鱼市开张典礼的日期移后。第二，深恐皇族约克公爵和公爵夫人以及布赖恩。勃鲁国王陛下分别前来访问一事，会招致来自左右两方面的反对。第三，新峻工的伯格码头区的大歌剧厅和霍金斯街的皇家剧场，存在着职业的礼仪与职业的竞争之间的矛盾。第四，由于内莉。布弗里斯特的那种非理性、非政治、不时兴的容貌会引起观众的同情；内莉。布弗里斯特身穿非理性、非政治、不时兴的白色衬衣，当她表演时一旦将衬衣袒露出来，会撩拨观众的情欲，令人担心会使观众神魂颠倒。第五，不论是挑选适当的乐曲还是从《笑话共赏集》（共一千页，每个笑话都令人捧腹）里选一些滑稽的隐喻都是困难的。第六，这首主题歌不论谐不谐音，都与新任市长大人丹尼尔。塔仑、新任行政司法长官托马斯。派尔以及新任副检察长邓巴。普伦凯特。巴顿的姓名有联系。
Results indicated that the resultant cybrid cells are characterized by the appearance of hemoglobin and its expression might be served as a marker for the cybrids.
果与电镜和流式细胞光度计的实验结果一致，表明珠蛋白基因产物血红蛋白住杂交细胞中，能够表达并成为融合细胞的标志。
In this paper, we have given a new effective algorithm for root bound of the algebraic expression based on the resultant of the algebraic polynomial, also, with MAPLE ′ s help, we have realized this new algorithm in our microcomputer.
得出了代数数根界的一个基于结式的有效实现算法，并且已用计算机代数系统MAPLE在微机上实现。