Immunohistofluorescent staining was used to identify the binding specificity of GEBP11 in gastric cancer tissues. 5. GEBP11 was labeled with ~(99)Tc~mO_4~- using direct labeling method, and was labeled with ~(131)I using NBS labeling method. Then the labeled peptides were validated for radiochemical yield, specific activity, and in vitro. stability. 6. The bioactivity of ~(99)Tc~m-GEBP11 or ~(131)I-GEBP11 was validated by cell receptor autoradiography in cultured HUVECs. 7. Receptor binding assay was performed in vitro. to analyze quantitatively the binding specificity and affinity of GEBP11 to HUVEC, the receptor intensity on HUVEC, and the difference of affinity of GEBP11 binding to Co-HUVEC and HUVEC. 8. The radioactivity of all organs of nude mice injected with ~(131)I-GEBP11 was determined and the radioactivity of all organs per g was calculated to analyze the specific distribution of ~(131)I-GEBP11.9. Gamma camera images of nude mice bearing tumor xenografts of human gastric carcinoma injected with ~(99)Tc~m-GEBP11 was obtained 0.5-24 h after being injected to identify the targeting ability of labeled peptide to tumor tissues. 10. MTT assay on HUVECs and antitumor assay in nude mice bearing tumor xenografts of human gastric cancer were performed to evaluate the effects of ~(131)I-GEBP11 on HUVECs and xenografts. 11. Immunohistochemical staining was used for MVD counting in tumor. 12. H. E. staining, blood cells analysis and blood biochemical indicator analysis were performed to investigate the injury of liver and bone marrow. 13. Tube formation assay in matrigel, CAM angiogenesis assay and angionenesis induced by matrigel in mice were performed to identify the effects of GEBP11 on angiogenesis.
1、通过蓝色噬斑形成实验及GEBP11、IN11噬菌体在荷瘤裸鼠体内的竞争抑制实验检测噬菌体体内归巢的特异性；2、免疫荧光技术检测GEBP11在荷瘤裸鼠体内的结合特异性；3、免疫细胞化学或荧光检测GEBP11在Co-HUVECs中的定位及内化；4、免疫组织荧光检测GEBP11在人胃癌组织中的结合特异性；5、采用直接法~(99)Tc~mO_4~-标记GEBP11，采用NBS法~(131)I标记GEBP11，纸层析法测定标记率、放射化学纯度等；6、放射自显影鉴定GEBP11同位素探针的结合活性；7、受体放射配基结合分析实验鉴定受体亲和力及受体细胞密度；8、同位素示踪技术检测~(131)I-GEBP11在荷瘤裸鼠体内的生物学分布；9、SPECT成像技术进行荷人胃癌裸鼠体内~(99)Tc~m-GEBP11显像；10、MTT细胞增殖实验、荷瘤鼠抑瘤实验评价~(131)I-GEBP11对内皮细胞及荷瘤鼠肿瘤的抑制能力；11、免疫组织化学染色计数瘤组织MVD；12、病理学HE染色、血液血细胞分析及生化指标检测观察肝脏损伤及骨髓抑制情况；13、Matrigel管状结构形成实验、鸡胚绒毛尿囊膜血管生成抑制实验、小鼠体内Matrigel Plug诱导血管生成实验分析GEBP11对血管生成的影响；14、MTT细胞增殖实验、细胞周期及凋亡分析、细胞侵袭迁移实验、细胞粘附实验探讨GEBP11抑制血管生成的细胞机制；15、基因表达谱芯片技术筛选GEBP11短肽作用Co-HUVECs后的差异表达基因。
Conventional radiochemical methods such as alpha spectrometry, neutron activation analysis, liquid scintillation system, fission track and gamma spectrometry for the quantitative determination of neptunium and plutonium often require complicated and time-consuming sample preparation and separation procedures.
研究环境中铀、镎、钚等长寿命放射性核素的精密测定，对保护公众安全和保护环境，对了解核设施的历史操作情况和现状，都具有重要意义。环境样品基体复杂，铀、镎和钚等锕系元素含量非常低，在复杂的生态条件下，这些核素常常具有复杂多变的化学形态，要准确测定其含量，必须要有灵敏可靠的检测方法。
Activation analysis and non-radiometric method have been used to measure long-lived fission product nuclides. Their sensitivity is superior to that of traditional radiochemical analysis.
活化分析以及非放射的仪器分析方法被用来测量长寿命裂变产物核素，其分析灵敏度优于传统的放化分析法。
Determination of multi-elements in biological materials by means of radiochemical group separation neutron activation analysis
放化组分离中子活化分析法测定生物样品中多种微量元素
Samples were determined by uranium chemical analysis and radium radiochemical analysis and it is obvious to note that the radon content released from thermal effect in minerals is positively correlated to the uranium and radium content.
样品又做了化学铀分析和放射化学镭分析。明显看出热释氡量与铀、氡含量成正相关关系。
Preparation of nitrate-H_2SO_4-graphite intercalation compounds by a two-step intercalation method Radiochemical analysis of strontium-90 in water & Extraction chromatography by di - (2-ethylhexyl) phosphoric acid
GB/T6766-1986水中锶-90放射化学分析方法二-（2-乙基己基）磷酸萃取色层法
Radiochemical analysis of cesium-137 in water
GB/T6767-1986水中铯-137放射化学分析方法
Radiochemical analysis of am in irradiated fuel
辐照后核燃料中镅的放化分析
Instrumental and radiochemical neutron activation analysis of layered chondrules from Allende meteorite
阿伦德陨石中壳层球粒的仪器及放射化学中子活化分析
After checking carefully it demonstrates that this method can replace the traditional radiochemical analysis in water sample monitoring.
经过仔细地检验，表明这个技术可以取代传统的放化分析方法，应用于环境监测之中。
Radiochemical analysis of~(79) se in Iaw high level liquid waste
放化分析高放废液1AW中的~（78）Se
Some new work on the Cherenkov counting technique and rapid radiochemical analysis has been published.
契连可夫计数技术以及快速放化分析方面也有新的工作发表。