NHE is proposed to come from the chemical redox reaction, i. e., NO-induced oxidation of hemoglobin in the film, coupled electron transfer process of hemoglobin.
其中位于0.172V的前峰可能是产生于与血红蛋白电子传递过程相偶联的一个化学反应，即一氧化氮引起血红蛋白的氧化。
To construct a retroviral vector carrying immortalizing gene hTERT, screen gene puro, reporter gene EGFP and recombination site sequence LoxP for reversible cell immortalization. Linearized pBABE-puro was cut by restriction enzyme Nhe Ⅰ and ligated with loxP double strands to construct a vector pBABE-puro-lox.
目的 探讨以hTERT作为永生化基因、嘌呤霉素乙酰转移酶基因puro作为筛选基因、加强型绿色荧光蛋白EGFP作为报道基因、loxp作为重组位点构建可回复性永生化逆转录病毒载体，为建立可回复性永生化细胞株莫定基础。
The synthesized siRNA can inhibit the expression of NHE-1 and can protect HKC from ischemia reperfusion injury induced by antimycin A.
其机制可能通过抑制NHE-1表达，延缓细胞内Na+的积聚，减轻细胞内钙离子的超载，抑制损伤细胞的线粒体膜电位下降，减少细胞的凋亡。
Methods: The core antigen gene of HBV was amplified by PCR. A MCS of Nhe I was created in the 79~80AA of HBcAg by gene mutation. This gene was directly inserted into the downstream (between the MCS of EcoR I and BamH I) of CMV promoter in the pIRES2-EGFP.
采用PCR法扩增HBcAg基因，并通过基因突变在79~80位氨基酸处生成一个Nhe I酶切位点，然后将这段基因连接到真核表达载体pIRES2-EGFP启动子下游的EcoR I和BamHI之间。
Results Cells transfected NHE1 antisense gene compared with parent cells, Proliferation index decreased, the clone formation capacity in two layer soft agar play down, apoptotic rates increased, growth curve slowers. Format carcinoma capacity in nude mouse disappear.
结果 NHE1反义基因能使SGC7901胃癌细胞生长速度减慢，双层软琼脂集落形成能力、体外生长增殖能力降低，细胞凋亡率增加，裸鼠体内成瘤能力显著降低。
The electron microscope showed increased number of mitochondrion and size of the transfected SGC7901 gastric cancer cells with NHE1 antisense gene, expanded Golgi apparatus, rough endoplasmic reticulum and endoplasmic reticulum and strengthened heteropolarity.
透射电镜下细胞线粒体数量增多、体积增大，并可见髓鞘样变，粗面内质网、高尔基体较发达，内质网扩张，核偏向一侧，极性增强。
Methods We use the cultured myocardial cells of DM rats and deal in ischemia/reperfusion condition, simulate myocardial cell I/R damage, to observe dynamic change of cytoplasm Ca2+ concentration of single cell in the ischemia/reperfusion condition, and to observe the influence of EIPA to Ca2+ concentration when be used in different period, meanwhile to survey the change of cell area and survival rate.
利用培养的糖尿病鼠心肌细胞进行缺氧/复氧处理，模拟心肌缺血/再灌注损伤，动态监测单个心肌细胞在缺氧/复氧时Ca2+浓度的变化和阿米洛利(EIPA，一种NHE阻滞剂)对其细胞面积和细胞生存的影响。
Inhibition of the pathway was reversed by enforced alkalinization or overexpression of NHE-1, leading to a restoration of apoptosis.
高度碱化或NHE-1 过度表达引起细胞凋亡恢复可翻转对该途径的抑制作用。
Following allosteric activation by intracellular acidification, NHE exchanges extra - cellular sodium for intracellular hydrogen with stoichiometry of 1:1.
细胞内酸化变构激活NHE，以1：1的比例将胞外的钠离子与胞内的氢离子进行交换。
Calcineurin B homologous protein (CHP) is an essential regulation cofactor for NHE.
钙调磷酸酶B同源蛋白（CHP）是NHE的一个活性调节亚单位。
The geographic science may be renew by using nhe " summary science " theory which be formed by the convergence of the natural science and the social science and the synthetic integrated method which be formed by the combi-nation of the qualitative and quantitative research methods of man-land relationship.
运用自然科学与社会科学相汇合而形成的概括科学理论和研究人地关系的定量与定性相结合的综合集成法，将会更新地理科学。
Methods: The expression of NHE 1 mRNA in human lung cancer tissues as well as normal lung tissues was measured with the method of in situ RNA molecular hybridization (RNA IMH).
方法：采用RNA原位分子杂交法（RNAIMH）检测了27例人肺癌组织和正常肺组织NHE1mRNA的表达。
NHE expression is significantly increased in tumor cells, it is closely related to the tumorigenesis and metastasis.
NHE1在肿瘤细胞中的表达水平明显增高，与肿瘤的发生和转移有着密切的关系。
It is the insufficient of national health expenditure (NHE) that led the loss of health development of China. Meanwhile, the unbalanced between the development of health expenditure and that of economics mainly has effect on it.
目的卫生投入总体不足，卫生发展与经济发展的相对不平衡，是中国卫生事业管理中存在的主要问题。
Study of the nude mice-human ependymoblastoma xenograft model NHE-2
人脑室管膜母细胞瘤组织&裸小鼠移植模型NHE-2的研究
Objective To construct the antisense expression vector of human Na + H + exchanger 1 (NHE 1).
目的构建人Na+H+交换蛋白1（Na+H+exchanger1，NHE1）基因反义真核表达载体。
However, when cells treated with NHE1 inhibitor EMD87580, they could still form the EBs and proliferate when cell clusters adhered on the culture plate, but cells were unable to differentiate.
而经过抑制剂处理的P19干细胞尽管能够形成类胚体且贴壁培养后细胞仍具有增殖活力，细胞团周边也较整齐，但未出现向心肌细胞分化的现象。
SHV beta-lactamase gene was detected by SHV-PCR, and SHV-ESBL were further examined by PCR-Nhe ⅰ method.
用SHVPCR检测SHVβ内酰胺酶基因，进一步用PCRNheⅠ酶切检测SHVESBL。
In addition, added scan potentials (- 100~- 300 mV vs. NHE) not only increase the numbers that PSAF stores the molecules, but also accelerate storing rate.
此外，外加扫描电位（-100～-300vs.NHE）不仅能提高PSAF储存有机小分子数量，而且还能加速储存速率。
In order to adjust the distance between compressor stations or increase the transporation quantity of gas trunk pipeline, it is often necessary to lay a certain length of parallel pipe and variable diameter pipe in nhe design of gas trunk pipeline.
在输气管线设计中，为了调整压缩机站之间的距离和增加管线的输量，常需要在管线中敷设一定长度的副管或变径管。