To identify Listeria innocua in food, a PCR-Denaturing High-Performance Liquid Chromatography assay was performed in this study.
应用PCR结合变性高效液相色谱技术，建立食品中英诺克李斯特氏菌的快速检测方法。
The good specificity of this method was found in the study. Also, it can detect and identify Listeria innocua quickly and accurately by a PCR-DHPLC.
试验结果表明该方法具有很好的特异性，经PCR-DHPLC可检测食品中英诺克李斯特氏菌。
Specific primers for specific gene of Listeria innocua were selected to conduct the PCR-DHPLC assays. The specific testing was performed with 41 strains.
根据英诺克李斯特氏菌的特有基因设计特异性引物，PCR扩增的产物经DHPLC技术进行快速检测，并以41株参考菌株做特异性试验。
Irradiation at dose lower than 2.0kGy have no significant effects on sensory qualities, content of crude protein and amino acid （p〉 0.05）, and decrease 2 - 3 log of microbial in the mixture blanched peanut and celery and above 6 log of pathogens of Listeria innocua and Salmonella enteritidis inoculated.
值分别为0.284和0.296kGy，2.0kGy以下的辐照剂量对其感官品质和蛋白质的营养品质没有明显的影响。2.0kGy剂量能使冷食菜中的微生物降低10^-2～10^-3，使接种于其中的致病菌降低10^-6以上，能有效地提高芹菜花生冷食菜的卫生安全。
Various genomic DNAs of Listeria innocua, Listeria weshimeri, Salmonella, Staphylococcus aureus, Bacillus subtilis, Escherichia coli and Proteus were used as negative controls to confirm the specificity of duplex real-time PCR assay.
结果 成功构建了重组质粒标准品，并运用5'、3'端分别标记FAM、TAMRA的hly基因探针和5'、3'端分别标记HEX、TAMRA的ipaH基因探针成功建立了单增李斯特菌与志贺菌同步荧光定量PCR检测方法。
HORIZONTAL METHOD FOR THE DETECTION AND ENUMERATION OF LISTERIA MONOCYTOGENES.
单核细胞增多性李司忒氏菌的横向检查和计算法。
The lab is capable of quarantine hazardous biology such as: Phytophthora sojea, Tilletia controversa, Bursaphelenchus xylophilus, Sorghum halepense, Acanthoscelides obtectus, Bean Pod mottle virus, and practising diagnosis akabane disease, leukaemia bovum, paratuberculosis and other hazardous animal disease; detection of listeria monocytogenes, staphylococcus aureus and salmonella etc in food; examination of transgenic products such as soybean, rapeseed, corn etc and detection of bovine, sheep and goat -derived for preventing bovine spongiferm encephalopathy.
检测的项目主要有：大豆疫病、小麦矮腥黑穗病菌、松材线虫、假高梁、菜豆象、菜豆英斑驳病毒等植物危险性有害生物，赤羽病、白血病、副结核等动物危险性有害生物，单增李斯特氏菌，金黄色葡萄球菌，沙门氏菌等致病性食品微生物，大豆、油菜籽、玉米等转基因产品，以及预防疯牛病的牛羊源成分检测等。
The plant Quarantine Lab had intercepted more than 200 quarantine pests such as: Tilletia indica, Tilletia controversa, Bursaphelenchus xylophilus, sorghum halepense, Acanthoscelides obtectus, Sternochetes mangiferae, Bactrocera dorsalis, Coptoermes curvignathus for nearly 5000 batches; The Animal Quanantine Lab had intercepted about 20 cow which had some pathogens evidence of infectious disease such as: bovine infectiouse rhinotracheitis, leukaemia bovum, paratuberculosis and so on; The food microorganism lab had detected over 300 unqualified products such as Salm-Sury, Listeria monocytogenes, Mycobacterium paratuberculosis etc.
多年来，分中心各实验室切实履行严格把关的职责，植物检疫共截获大豆疫霉病菌、小麦印度腥黑穗病菌、小麦矮腥黑穗病菌、松材线虫、假高梁、菜豆象、芒果果核象甲、桔小实蝇、大家白蚁等国家禁止进境的危险性有害生物220 多种近10000 批次；动物检疫发现感染牛传染性鼻气管炎、牛白血病、副结核等传染病的疫牛20 多头；食品微生物检测共检测沙门、单增、副结核的不合格商品300 多批；分子生物学实验室为国内企业出具非转基因证书和不含有牛羊源成分证书50 余份，使相关的产品能够顺利出口到欧盟、韩国和日本等国。
In recent years, medical science progresses, the Gram-positive bacilli have a more in-depth study. Here on Gram-positive bacilli in the mononuclear cells of Listeria monocytogenes, Diphtheroid bacilli, erythema silk erysipelas bacteria of Clostridium perfringens and Clostridium difficile, Bacillus anthracis and Nocardia infections, Detection and treatment are reviewed.
近年来，医学科学不断进步，对革兰阳性杆菌有了更深入的研究，为此就革兰阳性杆菌中的单核细胞增生性李斯特菌、红斑丹毒丝菌、产气荚膜梭菌、艰难梭菌，炭疽杆菌以及诺卡氏菌的感染、检测方法及治疗作一综述。
Microorganisms in the lateritic profiles of Guantoudong gold deposit in Lianxian, Guangdong were cultured, isolated and identified. The microorganisms include bacteria such as Bacillus, Enterobacter, Pseudomonas, Lactobacillus, Actinetobacter Aeromona, Listeria, Agrobacterium, Corynebacterium, Moraxella, and fungi such as Penicillium, Alternaria, Saccharcomyces, Mucor.
对广东省连县观头垌红土型金矿中的微生物进行培养、分离和鉴定，获得的主要微生物类型有芽孢杆菌属、肠杆菌属、假单孢杆菌属、乳杆菌属、不动杆菌属、气单孢杆菌属、利斯特氏菌属、土壤杆菌属、棒状杆菌属、莫拉氏菌属等细菌，青霉菌属、交链孢霉属、芽枝霉菌属、酵母菌属和毛霉属等真菌以及化能自养菌。
It is caused by the germ Listeria monocytogenes, which has a special ability to survive in adverse conditions.
它是由细菌李斯特菌，其中有一个特别的能力，生存在恶劣的条件。
The reports say that more than 1, 500 people are infected by listeria every year in America.
报告显示，美国每年有1500多人感染李氏杆菌，翻译中，请稍等。。。
The United States Food and Drug administration and the Department of Agriculture have published studies on listeria.
美国食品和药品管理局和农业部已经公布了对李氏杆菌的研究报告。
This method is useful for the investigation of listeria in food
此外，由于该菌在4℃冰箱保存的食物中也可生长繁殖
Objective To establish a fast and accurate method to examine the Listeria monocytogenes in foods.
目的：建立一种快速准确检验食品中单核细胞增生李斯特氏菌的方法。
The listeria germ has been found in many different species of animals, birds, fish and crustaceans.
该李斯特细菌已发现在许多不同种类的动物，禽鸟，鱼类和甲壳类动物。
Objective: To study the sensitivity of PCR method for the detection of Listeria monocytogenes in food.
目的：研究PCR检测不同食品中产单核李斯特菌的灵敏度。
Other common causes include salmonella, listeria, shigella and clostridia.
其他常见的原因包括：沙门氏菌，李斯特菌，志贺氏菌和梭状芽胞杆菌。
From the public health point of view, Listeria monocytogenes as one of the most important food-borne pathogen, it has been paid much more attention of the people since 1990s. Many experts and scholars at home and abroad study the biological characteristics, detection technology, pathogenicity gene, virulence factors and epidemiological of Listeria monocytogenes.
从公共卫生学角度，单增李斯特菌作为一种重要的食源性传染病的致病菌，自20世纪90年代以来，更加倍受人们的关注，国内外许多专家学者针对单增李斯特菌的生物学特性、检测技术、致病基因及其毒力因子、流行病学进行了研究。
Objective The Listeria monocytogenes are widely distributed in nature and can be found on decaying vegetable and in soils, animal feces, sewage, silage, and water.
中文摘要：目的单核细胞增生性李斯特氏菌(Listeria monocytogenes，LM)广泛存在于自然界，如腐败的蔬菜、土壤、动物粪便、污水、储存饲料。