In order to meet the different needs of heterologous gene expression, we go on to construct the pHR series vectors.
为了使构建的载体能适应不同类型的外源基因的表达需要，进一步构建了pHR系列载体。
In order to investigate the effect of acupuncture stimulation on skin blood flow and the relationship to calcitonin-gene related peptide.
中文摘要本研究的目的在於探讨针刺对皮肤血流的效应，及其与降钙素基因相关胜肽（calcitonin-gene related peptide，CGRP）的关系。
Hepatitis C; Chronic/immunology Hepacivirus/immunology Gene order Lipoproteins/metabolism Apolipoproteins B/metabolism
肝炎；丙型；慢性/免疫学；肝炎病毒/免疫学；基因顺序；脂蛋白类/代谢；载脂蛋白B类/代谢
In this project, we will explore a novel Tat peptide mediated gelatin-siloxane nanogels as gene carrier in order to optimize the transfection and expression efficiency in vitro and in vivo. A challenging task is to use sol-gel and coagulated
本课题的创新点在于将通过溶胶-凝胶、共凝聚等方法制备Tat-壳聚糖纳米颗粒和Tat-明胶-硅氧烷纳米复合凝胶作为质粒DNA的载体材料，并着重探讨Tat介导的纳米凝胶的穿膜和核定位的能力，从而为肿瘤的基因治疗的进一步深入研究提供实验数据和理论依据。
In order to enhance the expression level of scu--PA in E. coli, studies have deen done on the alteration of 3'end of scu--PA cDNA gene and on the use of DnaY gene, which product can improve the translation of rare Arg condon in scu--PA cDNA gene.
为了获得人尿激酶原cDNA在E.coli中的高效表达，我们在其3`端改造，并在利用稀有Arg密码子即DnaY基因的识别方面展开了研究，表达产物由原来占菌体总蛋白的不到1％提高到15％。
In order to increase the fusion expression to meet the needs of industrial application, the apidaecin gene was modified according to the codon bias of Lactococcus lactis.
为了进一步提高产量，达到工业化生产的要求，本研究根据乳酸乳球菌的密码子偏嗜性，对原来的apidaecin基因进行了改造。
To study the relationship between the expression of mdrl gene and chemotherapeutic drug resistance in breast cancer, and to select efficiency methods detecting MDR of breast cancer in clinic, and in order to make certain of the scheme of chemotherapeutic drugs.
对乳腺癌mdr1基因的表达与化疗药物耐药的相关性进行研究，以筛选临床乳腺癌MDR检测的有效方法，并且籍以确定化疗药物的治疗方案。
Objective To obtain the encoding gene of human transforming growth factor-beta 3 in order to develop an effective antiaging skin drugs.
目的：获取人转化生长因子β3的编码基因，开发抗衰老的皮肤药物。
The capripoxvirus live attenuated vaccine strain G14-STV44-55 was cultured with sheep testicular cell, and the genomic DNA was extracted from the virus strain, and a pair of specific primers were desined in order to amplify a TK gene.
用绵羊睾丸细胞培养山羊痘病毒疫苗株G14-STV44-55，提取其基因组为模板，设计TK基因的特异性引物并进行PCR扩增，获得了2405bp的DNA片段，并将PCR产物克隆至pGEM-T Easy载体。
The aim of this thesis was to detect the expression of CLC-3 gene in order to find their relation with cervical cancer and the prognosis by RT- PCR. Purpose To study the expression and clinical significance of CLC-3mRNA in cervical cancer.
CLC-3在宫颈癌组织中的表达情况尚不清楚，故本研究的目的是利用逆转录-聚合酶链反应方法探讨电压门控性氯离子通道(CLC-3)在子宫颈癌组织中的表达及临床意义。
In order to rich rabies virus gene bank, sequencing and analyzing parts of the two rabies virus street strains isolated from different animals in China.
本研究为了丰富我国狂犬病基因库，对我国不同动物中分离到的两个野毒株进行基因组部分序列的测定和分析。
Methods Ten mature Wistar rats were divided into normal control group (5 rats) and adenovirus (E1, E3-Deleted and carried math1 and enhanced green fluorescent protein report gene, Ad-Math1-EGFP) scala vestibuli transfer group (5 rats). Right ears of the Ad-Math1-EGFP transfering group rats were deliveried 5μl Ad-Math1-EGFP (physical tite 2.1×10^11v. p./ml) into cochleas through the way of drilling scala vestibuli of cochlear basal turn. As a control, the normal group received nothing to inner ear. In order to estimate functional condition of vestibule and cochlea, the click-evoked potentials on the surface of the cervical dura mater, auditory brain stem response and swimming time were recorded in all rats at 7 days after treatment, and then histologic and morphologic observation were carried out after animals were sacrificed. Results All animals'morphologic observation showed that inner ear hair cells were normal after transfer.
将10只成年Wistar大鼠分为正常对照组和缺失E1、E3基因片段且构建有Math1基因和绿色荧光蛋白报告基因的复制缺陷型腺病毒(adenovirus-Math1-enhanced green fluorescence protein，Ad-Math1-EGFP)前庭阶导入组，每组5只，实验组大鼠在右耳通过耳蜗底回前庭阶打孔的方法导入物理滴度为2.1×10^11v.p/ml的上述腺病毒5μl，对照组大鼠不做任何处理。7天后对动物进行颈髓硬膜外短声诱发电位(click-evoked potentials on the surface of the cervical dura mater，CDM-CEP)、听性脑干反应阈值检测和游泳试验，评价前庭和耳蜗功能，然后将动物处死进行组织形态学观察。
Under various stressed conditions, not only the protein-coding genes, but also the non-protein-coding genes were induced for response. More and more re-searches showed that the transcripts of these non-protein-coding genes played important role in regulation of gene expres-sion. miRNA is one of the groups in these no-coding regulatory small RNAs. Recent findings showed that in order to resist the biotic and abiotic stresses, expression of microRNA genes will be induced and their transcripts can regulate gene expression by guiding target mRNA cleavage or translation inhibition.
研究表明，逆境胁迫不仅会诱导植物蛋白质编码基因的表达，也会诱导一些非蛋白质编码基因的表达，这类非蛋白质编码基因的表达产物在植物的生长、发育和应对逆境胁迫等过程中起到重要的调控作用。miRNA就是这类非蛋白质编码基因产物中的重要类群，研究发现，多种逆境均会诱导miRNA的产生，其作用是通过引导目的基因mRNA的降解和阻止翻译过程来调控靶基因，最终通过形态或生理上的变化达到对逆境的适应。
The main work and conclusion of the present study were as the following:(1) In order to testify the validity of the mitochondrial CO1 gene for species identification, we performed our research at different levels, such as Genus Culter, Subfamily Cultrinae and the East Asian endemic Cyprinid group.
本论文的研究内容可大致分为2个部分：(1)分别以鲌属、鲌亚科和东亚特有鲤科类群为研究对象，检测线粒体CO1基因在这些类群中进行物种鉴定的可行性。
In order to understand the function of the Rab protein in ciliates, the coding region of Eorab1f was obtained from macronuclear DNA of Euplotes octocarinatus. Sequence analysis revealed that the gene contains 624 bp and two in-frame TGA codons in the coding region.
为进一步研究该家族成员的功能，本研究从八肋游仆虫大核基因组中克隆得到Rab蛋白家族中一个新Rab蛋白基因(命名为Eorab1f)的编码区。
AIM:To explore whether cyclosporine A can regulate the expression of nometastatic gene23 H1 type (nm23-H1) in human choriocarcinoma Bewo cells, in order to seek new proof of treating trophoblast diseases.
目的：探讨环孢素A对人滋养细胞系Bewo的23号非转移性基因（nometastatic gene23）H1型即nm23-H1表达的调控作用，为治疗滋养细胞疾病提供新的依据。
In order to facilitate the detection of ScFv, the vector was further modified attaching sequences encoding c-Myc derived decapeptide to the 3'-end of ScFv gene.
进一步将c-Myc十肽连接在ScFv的羧基端，使所表达的ScFv易于检测。
In order to overcome the problem of multidrug resistance in human epidemic carcinomata anti-adriamycin cells (KB-A-1), the antisense and antigene oligonucleotides were used to investigate their effectiveness on inhibiting the mdr1 gene expression. The effectiveness of antisense or antigene oligonucleotide on inhibiting the multidrug resistance was detected by MTT colometric assay and ELISA.
中文题名反义与反基因寡核苷酸及其萘二酰亚胺偶联物对靶基因表达的抑制作用研究副题名外文题名 The inhibition of the targeted gene expression by the antisense or antigene oligonucleotides and their naphthylimide-conjugated derivatives 论文作者李军生导师张元兴魏东芝教授学科专业生物化工研究领域研究方向生物化学与分子生物学学位级别博士学位授予单位华东理工大学学位授予日期2001 论文页码总数106页关键词基因表达基因治疗反义寡核苷酸反基因寡核苷酸核酸馆藏号BSLW /2001 /Q78 /264 针对人表皮癌抗阿霉素细胞株（KB-A-1）的多药抗药性问题，本文从反义核酸和反基因核酸角度，通过MTT法检测细胞生长情况，ELISA法检测基因表达产物P-gp表达水平的变化，对寡核苷酸抑制肿瘤细胞MDR1基因表达的机制进行了探讨。
In order to show whether the synthesized HL gene was effective, a yeast expression vector was constructed.
为验证合成的HL基因能否表达，我们构建了HL的酵母表达载体，Western杂交表明HL基因能够在酵母中表达。
In order to express foreign genes in this host an expression secretion vector was constructed with the PCR-glaA gene promoter, terminator of A. nidulans trpC gene and pSUGV2 as a backbone.
为了研究外源基因在SG1菌株中的表达，利用上述PCR扩增的糖化酶基因启动子片段和构巢曲霉trpC基因的终止子序列，以pSUGV2为载体骨架构建了表达分泌型载体pSUGX1。