And ten to fourteen days after each inoculation, tail and eye blood were taken to perform ELISA for special antibody, and footpad swelling test for delayed-type hypersensitivity. After the third inoculation, mice were killed and spleens were taken to prepare single spleen cell suspension for lymphocyte proliferation assay, and footpad were evaluated by histology.
每次免疫后10～14天取小鼠尾血，末次免疫后取球后血进行ELISA血清抗体检测；末次免疫后进行小鼠足垫迟发型超敏反应试验；处死小鼠后，断足进行小鼠足垫H.E染色，取小鼠脾脏制成单细胞悬液进行脾细胞增殖试验。