The staining of Eosin B, a biological reagent, on the grafted PET fabric confirmed the existence of amide groups in the grafted surface.
文章用生物染色剂着色的方法辅助证明了接枝表面上酰胺基的存在。
In a weak acid buffer solution of HAC-NaAC, when cetylpyridinium bromide is mixed with a dyestuff ethyl eosin after anionic surfactant is added, the colour of the soluotion becomes deeper, and the max absorbtion wavelength is at 516 nm.
在弱酸性的HAC-NaAC缓冲介质中，将溴化十六烷基吡啶与乙基曙红染料溶液混合，加入阴离子表面活性剂，溶液颜色加深，最大吸收波长都在516nm处，且阴离子表面活性剂的浓度与溶液的增色程度呈良好线性关系。
Lung tissue sections were stained with hematoxylin and eosin and examined under a light microscope to perform the histopathological examination. We found that RSV infection could rapidly upregulate the lung expression levels of both TLR7 and TLR3 gene in a time-dependent manner, furthermore, the response of TLR7 to RSV (1 h) was prior to that of TLR3 (4 h).
我们发现，RSV感染早期能快速上调TLR7和TLR3的基因表达水平，与正常组相比，其升高有显著性差异，并与RSV感染之间存在时间依赖关系；TLR7的反应（RSV感染1h）早于TLR3（RSV感染4 h）。
Hematoxylin and Eosin Staining shows a normal intestine wall and good mucosa in normal group; ulceration formed and a widen mucosa under layer with a large amounts of fibroplasia and hyperplasy of granulation tissue in model group.
苏木精-伊红染色正常组可见正常结肠壁组织，黏膜完好；模型组溃疡形成，黏膜下层可见大量纤维组织增生而明显增宽，肉芽组织增生。
IgE levels in serum were measured at various time-points. Lung specimens were fixed in 10% neutrally buffered formalin and paraffin embedded. Deparaffinized sections (5um thick)were stained with hematoxylin and eosin and analyzed under a light microscope.
于致敏后7、10、14、17天即药物干预后0、3、7、10天取材，观察各组血清IgE的动态变化；同样小鼠分为以上各组，于致敏后第14天进行气道激发，激发后24～48h进行取材，观察各组血清IgE及肺组织病理改变，并用免疫组化方法定性判断肺组织IL-4的表达。
Results Hematoxylin and eosin stain showed pterygium was a disease mainly with proliferation. The P53 and P63 protein was expression in pterygium tissures. The expression of P53 and P63 was significantly higher in pterygium than in normal conjunctiva epithelium The positive rate of the P53 protein in pterygium was significantly higher than normal conjunctivae U=2.500, W=17.500, Z=-
结果：翼状胬肉组织上皮层均可见不同程度的增生，上皮下组织成纤维细胞增生活跃、新生血管增多，血管周围有炎性细胞浸润。P53、P63蛋白在翼状胬肉组织上皮层中阳性表达呈细胞核阳性；翼状胬肉组织中P53和P63蛋白的表达均明显高于正常结膜组织；P53蛋白在翼状胬肉和正常结膜中的表达差异存在统计学意义(U=2.500，W=17.500，Z=-4.467，P＜0.05)。P63蛋白在翼状胬肉和正常结膜中的表达差异存在统计学意义(U=2.500，W=17.500，Z=-3.967，P＜0.05)。
The rabbits were killed after 8, 24, 72, or 168 hours (n=6 for each group). The clamps was placed but never clamped in shamoperated rabbits (n=24). The lumbar segment of the spinal cord (L5 to L7) was used for morphological studies, including hematoxylin and eosin staining and a modified terminal deoxynucleotidyltransferase mediated dUTP biotin nick endlabeling staining method.
参照并改进Zivin方法建立兔脊髓腰骶段缺血再灌注延迟性瘫痪模型，比较各组动物不同时间点后肢运动功能及病理形态学变化；采用原位末端脱氧核糖核酸酶转移介导的脱氧尿三磷酸标记法检测神经细胞凋亡水平。
A corneal abrasion with a diameter of 7 mm was created centrally in both eyes with a trephine and subsequent rubbing off on the corneal epithelium with a 20% alcohol soaked swab. Photographic documentation and hematoxylin and eosin stained pathological section were performed when rabbits were killed at 0, 12, 24, 36, 48 and 72 h. Planimetry was performed, and the corneal photographs were analyzed with computer software.
将4组兔双眼角膜中央置入直径7.00mm的环钻，其内滴入20%乙醇约60s后用角膜刮匙刮去角膜上皮，制作角膜上皮缺损模型，术后每组滴用眼液频率均为3次／d，各组分别于术后12，24，36，48及72h行裂隙灯下荧光素染色照相及海德堡共焦激光角膜显微镜检查后各处死2只兔，取角膜做病理切片同时应用计算机图像处理系统测量角膜损伤后愈合面积。
Methods One week after sterotaxis injection into CA1 subregion of hippocapus with different doses of Aβ and normal saline, we evaluated the gliacyte reaction and the expression of IL-1 β and S100 β by means of immunohistochemical assay, hematoxylin-eosin staining, and Congo red staining.
以不同浓度Aβ溶液及生理盐水作海马立体定向注射后，采用免疫组织化学、HE染色及刚果红染色方法，显示胶质细胞反应及IL-1 β、S100 β表达情况，并在高倍显微镜下(×400)进行阳性细胞计数，方差分析和等级相关分析作显著性检验。
Xanthene compounds can interact with DNA. Eosin Y was selected to be a model compound.
呫吨类化合物与DNA可发生相互作用。
For a dye regenerated system composed of eosin ethyl ester iodonium salt (EOEtIPh2) triethylamine, the efficiency of sensitization is 4 times higher than of single system EOEtIPh2, at mean time the number of active radicals is also multiplicated and thus this system is of importance in practical application.
由乙基曙红碘翁盐/三乙胺组成的染料再生体系的染料敏化效率比单一体系高4倍左右，同时使活性自由基的数量增殖，这种体系在实际应用方面具有重要意义。
Results After seven days of culture, some BMSCs appeared typical morphological neuronal traits. Cell bodies appeared refractile, cone-like or spherical with long processes, NSE was expressed. Animals in group A and group B recovered their motor functions in a varing degree, the difference of BBB score was significant (P.05). Hematoxylin and eosin staining demonstrated that the lesion areas of the spinal cord in group A recovered better.
结果 诱导7d后有部分细胞具备神经元样细胞形态，胞体呈锥形或圆形，有较长单极或多极的突起，有NSE阳性细胞表达；细胞移植后1~5周，A、B组动物运动功能均有不同程度恢复，但B组恢复较慢，BBB评分差异有统计学意义(P.05)；组织形态学观察示A组损伤脊髓恢复较好。
Sixteenth week after operation, the animals were sacrificed by aeroembolism. Cartilage grading was observed by general observation and operation microscope. Specimens were procured from the weight-bearing portion of the femoral condylar, and evaluated according to a modified histological-histochemical grading system (0-1 point as normal and over 10 points as severe) by haematoxylin-eosin and Safranin'O/Fast Green stainings.
术后第，6周空气栓塞法处死动物，大体观察及手术显微镜下观察软骨表面分级；取股骨髁负重面软骨标本进行苏木精－伊红、沙黄染色及骨关节炎组织病理学评分（正常为0~1，重度为10分以上）、分级。
The liver tissue was stained with both hematoxylin-eosin and picrosirius; The severity of liver disease based on the score of necrosis and inflammatory reaction and fibrosis was assessed in liver biopsy specimens by a skillful pathologist and by image analysis system.
每位临床受试者给予100mg 13C-phe，禁食12h，研究多项试验参数在健康受试者和各级肝硬化患者间的差异，以及试验参数与临床生化指标和Child-Pugh计分的相关性。
In the first trial, combination of enzymatic digestion was used to prepare suspensions of spermatogenic cells from adult mouse testis, and then a modified discontinuous Percoll gradient centrifugation method (15%, 22%, 30%, 40%, 50%, 60%) was introduced to isolate spermatids from the cellular suspensions. The content of spermatids in each isolated fraction by Percoll method was determined by morphology (Wright-Giemsa stain) and flow cytometry analysis, and the viability of spermatogenic cells was assessed by using eosin Y exclusion test.
在第一部分试验中，首先利用连续3次组合酶消化成年小鼠睾丸制备睾丸细胞悬液，然后经6层非连续Percoll梯度离心法(15％、22％、30％、40％、50％和60％)分离，通过形态学和流式细胞术鉴定南京医科大学硕士学位论文各个Percoll组分中精子细胞的含量，并以伊红Y排斥试验测定细胞的存活率。
Results: IL-8 protein was located in the epithelium of viii. The expression of IL-8 was significantly increased in RSA group than that in the control group. Positive IL-8 cells was shown in decidua of RSA group, and its IL-8 level was higher than that in the control group. By hematoxylin-eosin staining. trophoblastic layer was found to get thinner, cells of trophoblastic layer denatured or necrotized, and turned acidophily, and the fibration of villous axis increased in RSA; decidual cells lost connection, a part of decidual cells appeared cytoclasis and turned more acidophilic, and nuclei disappeared in RSA.
结果：在绒毛组织中，IL-8蛋白定位于绒毛上皮细胞的细胞质内，且病例组的表达明显高于对照组；在蜕膜组织中；病例组蜕膜细胞的胞质内可见IL-8蛋白表达，且高于对照组；H-E染色可见病例组绒毛组织的滋养层变薄，细胞变性甚至坏死、嗜酸性增强，绒毛中轴纤维化程度增强；蜕膜组织中蜕膜细胞失去细胞间连接，部分蜕膜细胞解体、核消失。
In pH 5.0—5.4 HOAc-NaOAc buffer solution, clindamycin could react with Pd to form a 1∶1 cationic chelate, which could further react with halofluorescein dyes such as diiodofluorescein, erythrosine and eosin Y to form 1∶1 ion-association complexes. As a result, not only the absorption and fluorescence spectra were changed, but also the resonance Rayleigh scattering intensities enhanced greatly and new RRS spectra appeared. The three reaction products had characteristic RRS spectra and their maximum RRS wavelengths were located at 285, 287 and 321 nm, respectively.
在pH为5.0-5.4的乙酸-乙酸钠缓冲溶液中，克林霉素与钯形成螯合阳离子，它能进一步与二碘荧光素，赤藓红，曙红Y等卤代荧光素类染料反应形成1：1：1的三元离子缔合物，此时将引起吸收光谱变化和荧光猝灭，同时还导致共振瑞利散射的急剧增强并产生新的RRS光谱，钯-克林霉素与DIF，Ery和EY形成产物的最大散射波长分别位于285，287，32 1nm处，另外还有些较弱的散射峰存在。
It was found that the fluorescence quenching of eosin Y appears when it reacts with the cation monomer of a CSAA and a new stronger fluorescence is obtained when the ion-associates react with the micellae of CSAA.
研究阳离子表面活性剂在水溶液中与曙红Y的荧光反应，发现当CSAA单体与曙红Y形成离子缔合物时，荧光发生猝灭，而CSAA胶束与曙红Y作用又会出现一个新的、更强的荧光。
Plates with eosin methylene blue agar medium one of them inoculated with organism A, another inoculated with organism B and the last one without inoculation
三盘含有伊红甲基蓝洋菜培养基的培养皿一盘装有菌种A，一盘装有菌种B，另一盘无菌
When DNA exists, the fluorescence intensity of phenylfluorone、eosin B and EB come out with different increasing. The absorbance of UV spectra present a hypochromic effect when DNA concentration increasing. The CD spectra of DNA has a great change both in negative and positive spectra. In the agarose gel electrophoresis, the luminescence intensity of EB-DNA system is the same as phenylfluorone-DNA system and eosin B-DNA system, so in the quantitative determination of DNA, we could implore the phenylfluorone or eosin B rather than the EB, which offer us an important base of new reagent in the quantitative determination of DNA, which also afford us clue to search more sensitive probe of DNA.
在ct-DNA存在时，苯基芴酮、曙红B和EB的荧光强度都有不同程度的提高；紫外-可见吸收光谱随ct-DNA浓度的增加，表现出减色效应；二者均使ct-DNA的圆二色谱正负峰吸收强度有所增加；凝胶电泳实验中，苯基芴酮-DNA体系、曙红B-DNA体系与EB-DNA体系的发光强度不相上下，故在DNA的定量测定中，可用无毒副作用的苯基芴酮或曙红B代替有高致癌性的EB，为安全、减少环境污染定量测定核糖核酸提供了新试剂使用基础，也为寻找更合适的DNA分子荧光探针提供实验基础。