Objective To illustrate the distribution of ATP synthase b subunit in the tissue of Clonorchis sinensis adult and its subcellular mimical localization in HeLa cells.
摘要目的了解华支睾吸虫ATP合酶b亚基（CsATP-synt_B）的虫体组织定位，并以HeLa细胞为替代模型观察其亚细胞定位。
Liver flukes such as Clonorchis sinensis or Opisthorchis viverrini usually enter human`s gastrointestinal tract after ingestion of raw fish.
Clonorchis sinensis和Opisthorchis viverrini之类的肝吸虫一般在人摄取生鱼后进入胃肠道。
Western blotting analysis revealed that SjcTrx expressed protein has a good immunological reaction and specificity because it could be recognized by sera from mice infected with Schistosoma japonicum, and not recognized by sera from healthy mice, rat infected with paragonimus, mice infected with Clonorchis sinensis and mice infected with Trichinella spiralis. So, the SjcTrx recombinant antigen has a good immunological reaction and specificity The clone and expression of gene encoding thioredoxin of Schistosoma japonicum in Pichia pastori has been successful.
经Ni~(2+)吸附组氨酸标记的亲和层析法纯化蛋白后，Western blotting显示该重组蛋白可被日本血吸虫感染小鼠血清所识别，不被正常小鼠血清、斯氏狸殖吸虫感染大鼠血清、华支睾吸虫感染大鼠血清和旋毛虫感染小鼠血清所识别，表明SjcTrx重组抗原具有较好的免疫反应性和特异性。
The life cycle of Clonorchis sinensis has seven developing stages.
日本血吸虫的发育阶段有6期。
Clonorchis may persist in the biliary tree for decades, and prolonged infestation is associated with the development of cholangiocarcinoma.
华支睾吸虫可以在胆道系统中存在几十年，长期的感染可与胆管癌的发病相关。
Objective To clone and express the valuable Clonorchis sinensis antigen molecules which can be applied to the diagnosis of clonorchiasis.
目的寻找对华支睾吸虫病诊断有效的抗原基因，以便生物合成并研究其应用价值。
Our results supported That there was close relationship between the liverfluke Clonorchis sinensis and the development of cholangiocarcinoma.
本组结果支持肝吸虫病与胆管癌发生关系密切，具有因果关系的观点，发现有或无肝吸虫病的肝内周围型胆管癌病灶具有相同CT特征及肿
Objective: To research in the isoenzymes of Clonorchis sinensis from different hosts and to explore the various situation of isoenzymes of Clonorchis sinensis in different hosts.
目的：通过对不同宿主华支睾吸虫的同工酶研究，了解其在不同宿主中的变化情况。
Methods Serum enzyme activity and liver pathology were examined in dogs infected with different number of larvae of Clonorchis sinensis.
方法观察感染不同程度华支睾吸虫病实验犬的血清酶学及肝脏病理学改变。
Out of 19 cases were co-infected with Clonorchis sinensis and 1 caes was co-infected with Ascaris lumbricoides, Trichuris trichiura and Enterobius vermicularis.
结果查到粪类圆线虫杆状蚴、丝状蚴，在呕吐物中还查到虫卵；其中7例患者合并华支睾吸虫感染；1例患者合并蛔虫、鞭虫和蛲虫感染。
Results the infection rate of major intestinal parasite was34.03%, the hookworm, ascaris lumbricoides, trichuris trichiura, enterobius vermicularis and clonorchis sinensis accounted for6.7%, 0.33%, 0.65%, 39.15%and27.11%, respectively.
结果 人体肠道重要寄生虫总感染率为34.03%，其中钩虫、蛔虫、鞭虫、蛲虫、华支睾吸虫感染率分别是6.70%、0.33%、0.65%、39.15%、27.11%。
Objective To investigate the infectious status of Clonorchis sinensis metacercariae in freshwater fish in Shenzhen area, analyze its prevalent features and provide scientific basis for making effective control measures in Shenzhen area.
目的了解深圳市淡水鱼及人群华支睾吸虫的感染现状，分析其流行病学特征，为制订深圳市肝吸虫病防治对策提供科学依据。
Objective To construct prokaryotic recombinant plasmids of transcriptional co-activator gene of Clonorchis sinen sis, express and purify the recombinant protein and analyze its biological function.
摘要目的构建华支睾吸虫成虫转录辅激活因子(transcriptionalcoactivator ，TC)基因原核重组质粒，进行原核表达、鉴定及生物功能分析。
Molecular characterization of a novel phospholipase A_2 from Clonorchis sinensis
华支睾吸虫磷脂酶A2基因生物学特性的初步研究
The isoenzymes of malic dehydrogenase (MDH), glucose-6-phosphate dehydrogenase (G6PD), esterase (EST), and peroxidase (PO) of Fasciola hepatica and Clonorchis sinensis adults were separated and studied by disc electrophoresis.
本文应用聚丙烯酰胺凝液盘电泳对肝片形吸虫和华枝睾吸虫成虫的苹果酸脱氢酶、葡萄糖-6-磷酸脱氢酶、酯酶和过氧化物酶进行了分离和研究。
Another monoclonal antibody was found to be against the common antigenic determinant of Schistosoma, Paragonimus, Clonorchis in I-ELISA, The immunoglobulin subclass of the 4 monoclonal antibodies were identified as IgM.
4株单克隆抗体免疫球蛋白亚类鉴定均属小鼠IgM。
Hookworm, Ascaris lumbricoides, Trichuris trichiura, Enterobius vermicularis, Taenia solium, Clonorchis sinensis, Entamoeba histolytica and Giardia lamblia showed high infection rates and wide distribution in people.
其中，钩虫、蛔虫、鞭虫、蛲虫、猪带绦虫、华支睾吸虫、溶组织内阿米巴、贾第虫属于分布广泛、感染率高、危害较大的虫种。
The prevalence of Ascaris lumbricoides, hookworms and Trichuris trichiura was 9.55%, 19.73% and 11.48% respectively. The infection rate of Enterobius vermicularis and Clonorchis sinensis was 22.06% and 0.60% respectively.
其中，蛔虫、钩虫、鞭虫感染率分别为9.55%、19.73%和11.48%，蛲虫感染率为22.06%，华支睾吸虫感染率为0.60%。
Among these species, the respective infection rates for Ascaris lumbricoides, Enterobius vermicularis, Trichuris trichiura, and Clonorchis sinensis were 30.7%, 20.1%, 1.3% and 0.1%, respectively.
蛔虫感染率为30.7%，蛲虫的为20.1%，鞭虫、贾第虫的分别为1.3%和1.2%。
Bioinformatics Analysis of the Full-length Cathepsin D-like Aspartic Protease Gene from Clonorchis Sinensis Aspartic acid can be broken down in the KREBS CYCLE.
华支睾吸虫组织蛋白酶D样天冬氨酸蛋白酶全长基因的生物信息学分析在三羧酸循环中天冬氨酸可以被分解。