Some key processes influencing construction of cotton bacterial artificial chromosome library, such as preparation of plug, DNA partial digestion, selection of digested fragment, molecular ratio between insert DNA andvector, concentration of ligates etc, were explored.
以我国抗病、优质、丰产的棉花优良品种中棉所12号为材料，对棉花细菌人工染色体文库构建过程中的一些关键技术，如plug的制备、DNA的部分消化、酶切片段的选择、插入片段与载体的摩尔比值、连接产物的浓缩等进行了研究，建立了构建棉花BAC文库的适宜方法体系。
The stable expression of exogenous gene in E. coli chromosome had no effect on the bacterial growth and propagation.
外源基因在大肠杆菌染色体上的稳定表达并不影响细菌的生长繁殖。
As a new developmental vector system, the bacterial artificial chromosome (BAC) have several advantages: larger capacity, hereditary stability and easy handling, etc.
细菌人工染色体是一种新发展起来的DNA载体系统，它具有容量大、遗传特性稳定、易于操作等优点。
Optimized Method for Construction of Bacterial Artificial Chromosome Library in Cotton
棉花细菌人工染色体文库构建的方法优化
Construction of a Bacterial Artificial Chromosome Library of Cotton Cultivars
棉花品种细菌人工染色体（BAC）文库的构建
Bovine herpesvirus type 1 genome cloned as infectious bacterial artificial chromosome and replication of gN gene deletion mutant in vitro
牛Ⅰ型疱疹病毒感染性细菌人工染色体的构建和gN基因缺失病毒的细胞繁殖特性
The Rapid Modification of a Bacterial Artificial Chromosome Containing Mouse β - casein Gene with Red System
利用Red系统快速改构含鼠β-酪蛋白基因的细菌人工染色体
By RecA protein mediated homologous recombination method, we successfully deleted the HS-40 fragment from a bacterial artificial chromosome containing complete human α - globin gene cluster screened by our lab.
采用RecA蛋白介导的同源重组的方法，对本实验室筛选出的包含完整人α类珠蛋白基因簇的细菌人工染色体（BAC）DNA进行删除修饰。
Research on the Preparation of Bacterial Artificial Chromosome (BAC) Vector DNA
关于细菌人工染色体（BAC）文库载体DNA制备的研究
The Modification of a Bacterial Artifical Chromosome Containing Human α - globin Gene Cluster by Homologous Recombination
用同源重组法修饰含人α类珠蛋白基因簇的细菌人工染色体
When the chromosome was isolated direct from the bacterial cells it was likely already methylated and therefore transplantable due to it being protected from the cells restriction enzymes.
当提取出来的染色体很可能已经被甲基化，因此对其移植应该已经有针对细胞内限制性内切酶的保护。
Using the homologous cloning strategy with degenerate primers and chromosome walking techniques, two genes fragments encoding cellulase were cloned from one bacterial strain Rhizobium radiobacter isolated from the gut of H. parallela larvae.
本研究使用兼并引物同源克隆并结合染色体步移的方法，从1株暗黑鳃金龟肠道内的纤维素降解菌Rhizobiumradiobacter基因组内成功获得2个纤维素酶编码基因。
To research melanin synthesis and its accumulation mechanism, we constructed a Silky bacterial artificial chromosome (BAC) library. The library was used to study the two genes on chicken Chr.
为研究丝羽乌骨鸡体内黑色素合成和沉积的分子机制，本研究构建了丝羽乌骨鸡的基因组细菌人工染色体（BAC）文库。
Progress on Application of Bacterial Artificial Chromosome System in the Reverse Genetics
细菌人工染色体载体系统在反向遗传学中的应用研究进展
Results: After continuous disinfection for 50 generations, the resistance of the test bacteria to sodium dichloroisocyanurate, iodophor and quaternary ammonium salt increased and the Xba ⅰ PFGE cleavage map of the bacterial chromosome DNA presented certain changes.
结果，连续消毒50代后，试验菌对二氯异氰尿酸钠、碘伏、季铵盐的抗力增加，细菌染色体DNAXbaⅠPFGE酶切图谱均发生一定的改变；
Construction and Analysis of Bacterial Artificial Chromosome (BAC) Library of Nosema bombycis
家蚕微孢子虫细菌人工染色体文库的构建
A new vector system-BAC (bacterial artificial chromosome) has been developed into an important method for the construction of complex genomic library.
一种新型的载体系统&细菌人工染色体（bacterialartificialchromosome，BAC）已经发展成为构建复杂基因组文库的重要方法。
Bacterial artificial chromosome (BAC) libraries are the most convenient, reliable and widely used genomic libraries in genome research of organisms with highly complex genomes.
细菌人工染色体（BAC）文库是复杂基因组生物的基因组研究中最方便、最可靠、应用最广的文库。
Transformation is one of the most common manners invovled in gene recombination by integrating exogenous DNA to bacterial chromosome, thus causing changes in some biological features.
转化是细菌基因重组最常见的方式之一，转化的结果可使细菌的染色体整合上外源DNA，从而使其某些生物学特征发生变化，并能代代遗传。
Methods: The denatured bacterial chromosome DNA linked to microplate reacted to the probe which was labeled by photobiotin.
方法细菌染色体DNA经分离纯化并变性后，直接非共价结合于固相微孔板中，与标有光标生物素的DNA探针进行杂交。