Based on the values of maximum specific growth rate, biomass and viable cells count in different cultural conditions, the optimal growth condition was regarded as follows: Glucose level of the medium was 80 g/L ~100 g/L; The pH was kept constant at 5.9 with aqua ammonia and anaerobic condition was kept by sparged with nitrogen periodically; The temperature was set at 37°C and cultured for
casei Zhang较适宜的高密度培养条件为：培养基葡萄糖浓度为80 g/L~100 g/L，以氨水为中和剂使pH保持5.9，采用间歇通氮气的方法保持环境厌氧，分批培养方式下37°C保温发酵10 h~12 h后，L。
This paper based on the study of the foreign technology to develop a large steel egg-shaped sludge digestion tank, which adopt the A/O activated sludge method and medium temperature digestion method to make the sludge well anaerobic digested and steadied. After continuously churning up, the firedamp will be generated on the top of the egg-shaped digestion tank, which can be used to power generation and heating.
本文是在学习和研究国外技术的基础上，研究大型钢制蛋形污泥消化槽采用A/O活性污泥法，中温消化法，使污泥在蛋形消化槽中进行充分厌氧消化稳定，经不断搅拌，在蛋形消化槽顶部产生沼气，用于发电及供热，脱泥后污水进行澄清处理后排放，再利用，污泥加工后用于生产肥料及其它建筑材料，工艺处于国际领先水平。
Methods:The technique of anaerobic bacterium culture was used to investigate the change of the amount of the total acid and lactic acid of S. mutans, which was cultivated in medium containing chitosamine or without.
利用实验室厌氧菌培养法，观察变形链球菌在有或无甲壳胺的培养液中代谢后，总酸量及乳酸量的变化情况。
Bifidobacterium longum can also form colonies in anaerobic TYG medium after 5 days.
无氧环境下，在TYL培养基中生长的比在TYG培养基中快且多，而在含氧环境下则都无法生长。
However, the size and number of colonies are less than which in anaerobic TYL medium. It can not grow in aerobic TYL and TYG media. Bifidobacterium longum can tolerate radiation dose as high as 80 Gy, which is the total dose in clinical radiotherapy. Therefore, Bifidobacterium longum has the potential to be the vector for cancer gene therapy combining radiotherapy. Besides, this is the first study to investigate the tolerance of X-ray radiation for Bifidobacterium longum.
此外，从本研究中得知Bifidobacterium longum可耐受高达80 Gy的辐射剂量（单次照射，存活分率为22 ％；以分次照射每次给予2 Gy来估算，存活分率为67％），此剂量为临床放射治疗一般给予的总剂量，因此证实Bifidobacterium longum有很大的潜力做为癌症基因治疗合并放射治疗的载具，本研究也是首篇调查Bifidobacterium longum对放射线耐受性的研究。
At present, Japan, Denmark, the Netherlands, Germany, France, the United States and other developed countries were generally taken by the anaerobic treatment of livestock manure, but like India, the Philippines, Thailand and other developing countries have also built a medium-sized biogas plants processing livestock manure demonstration project, the introduction of new self-loop anaerobic technology.
目前，日本、丹麦、荷兰、德国、法国、美国等发达国家均普遍采取厌氧法处理禽畜粪便，而像印度、菲律宾、泰国等发展中国家也建设了大中型沼气工程处理禽畜粪便的应用示范工程，采用新的自循环厌氧技术。
The result showed that Bifidobacterium longum grows and proliferates best in anaerobic TYL medium. It belongs to lag phase before 12 hours, log phase during 12 and 36 hours, and stationary phase after 36 hours.
结果发现Bifidobacterium longum最容易在无氧的TYL培养基中生长增殖，从生长曲线得知，12小时前为迟缓期，12至36小时为对数生长期，36小时以后为稳定期。
The two-step fermentation was used and the results showed that the overexpression of malic enzyme catalyzed the reverse reaction from pyruvate to malate, which was impossible under general conditions. Succinate accumulated as the major product. Cells at the late exponential phase were inoculated to an anaerobic fermentation with 0.7 mmol/L IPTG in medium containing 18.5 g/L glucose. The final concentration of succinate and acetate was 12.84 g/L and 0.58 g/L, respectively. Formate and lactate were not detected.
采用两阶段发酵模式，结果表明：过量表达的苹果酸酶在NZN111体内催化了从丙酮酸到苹果酸的逆向反应，丁二酸是发酵过程中积累的主要有机酸，且当加入0.7 mmol/L IPTG诱导，初始葡萄糖糖浓度为18.5 g/L时，选择对数生长期后期的菌种以10%的接种量转入厌氧发酵，发酵结束时发酵液中丁二酸的浓度为12.84 g/L，对葡萄糖的收率为69.43%，乙酸为0.58 g/L，二者浓度比为22：1，没有检测到甲酸和乳酸。
The sucrose consumption rate of the activated sludge of the anaerobic tank and of the effluent from the brewery and from Nanjing municipal drainage was near 85% with the medium containing high content sucrose respectively.
其中啤酒厂厌氧活性污泥、啤酒厂排污处污泥及南京城市排水沟污泥的蔗糖消耗率均达到85%以上；
Method Drip inoculation, aerobic or anaerobic culture in selective medium were used to test each section of gastrointestinal flora qualitatively and quantitatively.
方法采用滴注接种法，在选择性培养基上需氧或厌氧培养后定性定量检测胃肠各段菌群。
Three transport systems are introduced, the liquid and semisolid agar medium tubes and the sterilized dry aerobic syringe, comparing with that of the sterilized dry aerobic tube for culture of anaerobic bacteria.
采用液体培养基管、半固体琼脂培养基管、无菌干空针与无菌干空管等方法同时送检厌氧菌培养标本；
Inoculated the above working bacterial suspension into the nutrient agar medium and anaerobic bacterium agar medium with daubing method and incubated them under anaerobic condition at 35 ℃, and used the culture medium without inoculated as control.
将工作菌液采用涂抹法接种于营养琼脂培养基和厌氧菌琼脂培养基，置厌氧条件35℃培养，用未接种的培养基做空白对照。
METHODS: Lactobacillus fermentum was inoculated in medium containing 1% sucrose and different concentrations of CPP-ACP and grew in anaerobic condition at 37 ℃ for 48 hours. The primary pH values and final pH values of the supernate were measured, then the △ pH was compared.
方法：将发酵乳杆菌加入含不同浓度CPP-ACP的蔗糖培养基中，厌氧培养48h，用精密pH计检测培养基上清液的pH值，计算pH的变化值ΔpH（初始pH值-终末pH值）。
Treating organic sewage sludge of small and medium-sized farms by anaerobic sludge bed has achieved good results.
中小型养殖场采用厌氧污泥床处理有机污水取得了较好的效果。
Choice of Beer Anaerobic Culture Medium
啤酒厌氧菌培养基的选择
Biogas Potential and Characteristics in Medium Temperature Anaerobic Fermentation of Sunflower Plate, Wheat Straw and Bean Straw
葵盘、麦秆和豆杆中温厌氧发酵产气潜力及其特性研究
Anaerobic fermentation technology has become a main method for the treatment of large or medium hogpen wastewater, the agricultural utilization of anaerobic fermentation slurry are significant to environmental protection and agricultural development.
厌氧发酵技术成为大中型猪场废水处理的主要手段，厌氧发酵液的农业资源化对环境保护和农业发展具有双重现实意义。
Agricultural utilization methods for anaerobic fermentation slurry of large or medium hogpen
大中型猪场冲洗水厌氧发酵后农业资源化途径
The preapplied high strength and medium strength anaerobic adhesives were made by filling superfine calcium carbonate or silicon powder as filler in preapplied anaerobic adhesives.
在预涂型厌氧胶甲组分中加入超细碳酸钙、硅微粉作为填料，可制得性能较好的高强度、中强度预涂型厌氧胶。
Sodium carboxymethyl cellulose was employed (CMC) to improve the culture medium plate method for purifying and filtration of the aerobic and anaerobic cellulose decomposing bacteria in the earth and wood samples in Han Dynasty tombs at Siyang county under burying circumstance;
本研究采用羧甲基纤维素钠（CMC）改良培养基平板法对泗阳汉墓埋藏环境中的土样及木样中的好氧纤维素分解菌、厌氧纤维素分解菌进行了筛选和纯化；