The first part, by using RPA, The cytokines'expression during the immunomodulation was analyzed.
第一部分，用RPA(RNA酶保护法，Rnase Protection Assay)方法详细分析了在免疫调变前后多种细胞因子在小鼠腹腔巨噬细胞中的表达情况。
The results showed that:(1) 5'-nucleotide contents in yeast autolysate was slightly increased by adding RNase frommalted barley roots; (2) higher pH did not improve the 5'-nucleotide contents even in the presence of RNase from malted barleyroots, but at the pH 8.0, the addition of Acalase could increase the final yield, soluble protein contents and 5`-nucleotide inthe presence of RNase from malted barley roots.
酵母核酸酶的pH稳定性和温度稳定性均比麦芽根核酸酶的要差。这说明自溶法生产酵母提取物时，即使外加5'-磷酸二酯酶不能有效提高呈味核苷酸的原因并非由于外加的5'-磷酸二酯酶性质不稳定，而是另有其它原因。
Here, we demonstrate suppression of nonsense mutations in the luciferase gene in E.
而且，PNA的使用不会激活RNase H的活性。
The amplified fragments were cloned and sequenced. The sequence analysis revealed that this fragment contains 5364 nucleotides and shares over 70% similarity at both the nucleotide level and amino acid level with reported isolates of SCBV. The sequence has two complete open reading frames, which resembles to SCBV ORFI and ORF2 organizationally, but the open reading frame of RNase H was absent compared with SCBV.
该序列具有SCBV基因组结构特征，含有2个完整的ORF，与Gen Bank中已报道的2个SCBV分离物之间的核苷酸及氨基酸序列同一性均大于70%，但该序列与完整的SCBV基因组全序列相比，不具备完全的ORF3，缺失了对病毒复制过程极为重要的功能性基因RNase H的序列区段。
Properties of purified RNase TCS were investigated.
关于栝楼核糖核酸酶的性质已经研究。
Homology analysis showed that the amino acid sequence between putative peptides from above S gene fragments and that from S-RNase of rosaceous plant were 67%-96% of homology. Almond should be assigned to plum subspecific groups with cherry and apricot.
同源性分析表明，获得的10个氨基酸序列与蔷薇科中李亚科S-RNase的氨基酸序列同源性达67%～96%，扁桃与樱桃、杏应同属于李亚科。
In chapter four we managed to prepare the carboxyl terminal RNase III domain of fission yeast dicer in E. coli and detected its siRNA-generating activity.
反应体系中His-RNase Ⅲ和底物双链RNA的用量以及Mn2+浓度三者之间必需保持合适的比例，改变任何一种因素都会影响esiRNA的得率。
By using red blood cell broken-up solution, proteinase K and RNase, total DNA with high quality was extracted from the egg membranes in the incubated eggshells of two pheasant species, Reeves's Pheasant Syrmaticus reevesii and Ring-necked Pheasant Phasianus colchicus from Dongzhai Nature Reserve in Henan Province.
本实验以河南董寨自然保护区白冠长尾雉和环颈雉已孵化的卵壳为材料，利用红细胞破碎液、蛋白酶K及RNA酶等试剂，对卵壳膜内的总DNA进行了提取，建立了一种提取高质量DNA的新方法。
So the vegetable qualilies were improved. The reason was that Zn and B could increase photosynthetic rates, chlorophyll, sucrose, soluble sucrose, starch, zinc, boron, protein, nuclear, PAs, Put, Spd, Spm, IAA, GA〓 and ASA contents and SOD, POD, CAT, CA and NR activities, but reduce FAA, MDA, ABA contents and RNase activity in leaves, which inhibit membrane lipid peroxidation and promote plants growth.
这与锌硼提高了苦瓜叶片光合强度、叶绿素、蔗糖、可溶性糖、淀粉、Zn、B、蛋白质、核酸、多胺、腐胺、亚精胺、精胺、抗坏血酸、吲哚乙酸、赤霉素含量，以及碳酸酐酶、硝酸还原酶活性、超氧化物歧化酶、过氧化物酶、过氧化氢酶的活性，降低游离氨基酸含量、脱落酸、丙二醛含量和RNase活性，从而抑制膜脂过氧化、促进植株生长有关。
So the vegetable qualilies were improved. The reason was that Zn, Mo could increase protein, nuclear and PAs, Put, Spd, Spm, IAA, GA〓 and ASA contents and SOD, POD, CAT and NR activity, but decrease NO〓, FAA, MDA, ABA contents, AOR and RNase activity in leaves, which inhibit membrane lipid peroxidation.
这与锌钼提高了叶片蛋白质、核酸、多胺、腐胺、亚精胺、精胺、抗坏血酸、吲哚乙酸、赤霉素含量以及硝酸还原酶、超氧化物歧化酶、过氧化物酶、过氧化氢酶活性；降低了叶片游离氨基酸、NO〓、脱落酸、丙二醛含量和氧自动氧化速率以及RNase活性，从而抑制膜脂过氧化有关。
Coli 5S ribosomal RNA, demonstrating there is no contamination of DNase or RNase in the cambialistic SOD preparation.
进一步研究发现被DNA拓扑异构酶I松弛的闭合双链DNA也能被SOD作用产生线状产物。
When protein loading was in the range of 27-72μg, RNase activity band intensity remained unchanged.
随着上样量的增加，RNase活性逐渐增强。
Extracted DNA by CTAB, in proportion to the additions of PVP and β-mercap toethanol to the extraction buffer were capable of preventing browning from taking place in the extraction, Tris-Phenol (Stabilized with 0.1M Tris, pH 8.0)/Chloroform/isoamyl alcohol were used to remove protein and impurity in the second extraction, during crude DNA used to Isopropanol DNA and deterged by DNA detergent, without using RNase remove RNA, we detected the quality and concentration of DNA respectively for normal leaves and drought stress leaves by Agarose gel electrophoresis of genomic DNA and ultraviolet spectrometry photo.
用20%聚乙二醇(polyethylene glycol，PEG6000)模拟干旱，采取CTAB提取缓冲液，按一定比例加入PVP和β-巯基乙醇，用Tris平衡酚－氯仿－异戊醇除去蛋白质及杂质，在粗提时用异丙醇沉淀DNA，并用DNA洗液（10mmol/L醋酸铵，75%乙醇）洗涤DNA，在纯化时用乙醇沉淀DNA，且没有加入RNase去除RNA的方法，分别提取2个品种正常和干旱胁迫的苹果属叶片总DNA，并对提取的DNA进行琼脂糖凝胶电泳和紫外分光光度计检测。
Nucleic Acids Res, 2001, 29:5071.［6］TRANG P, LIU F, KIM J, et al. Effective inhibition of human cytomegalovirus gene expression and replication by a ribozyme derived from the catalytic RNA subunit of RNase P from Escherichia coli［J］.
和一些小RNA分子的5'端前导序列，M1RNA是RNase P的活性亚单位，是真正具有催化活性的基团［5，6］。M1RNA在体外即具有催化活性，它与自然底物之间的相互作用依赖于对底物结构的识别，而不是某些特殊的序列或核苷酸，这一点与其他类型的核酶不一样。
Tuberculosis of 2～3 weeks about 0.05g is washed by STE solution, then suspended with 300μl STE solution, homogenized for 5～10 times with homogeniser of 1 ml, lysozyme is added to 25mg/g bacteria, cultivate at 37℃ for 1 hour. SDS is added to 1%. water bath at 65～70℃ for 10～15 minutes. RNase A is added to 100μg/ml, cultivate for 1 hour at 37℃ to degrade RNA. Using phenal/chloroform extract the protein, the DNA is precipitated with 70% ethanol.
取结核杆菌的改良罗氏培养基2～3周培养物0.05克，用300μlSTE液悬浮，匀浆5～10次，加入溶菌酶至25mg/g菌体，37℃作用1小时，加入SDS至1％，65℃～70℃水浴10～15分钟，100μg/ml RNase A37℃作用1小时，以降解RNA，酚/氯仿抽提蛋白，乙醇沉淀DNA，得到的染色体DNA纯度为1.75，长度＞20kb，得率为200μg/g菌体左右。
We use isothermal titration calorimetry to study the binding thermodynamics of Mg2＋, Mn2＋, Ca2＋ to a type II RNase H from an archaeon:Methanococcus jannaschii.
本文通过等温滴定量热技术研究了Mg2＋，Mn2＋和Ca2＋与一种古细菌Methanococcus jannaschii中的II型RNase H结合的热力学。
It is very difficult to extract active RNA from Aspergillus niger because of the existance of complex cell wall and endo-Rnase activity.
由于复杂的细胞壁和内源酶活性的存在，所以要从丝状真菌中提取和纯化出有生物活性的RNA比较困难。
Based upon the characteristic reaction of S-RNA with FDNB which resulted in the attachment of the DNP-group only on the 5'-phosphomonoester end group of S-RNA, this report described experiments which utilized the DNP-group as label for the identification of the terminal nucleotides and their adjacent nucleotide sequences.
2对DNP-S-RNA的RNase I降解产物的双向电泳层析图谱进行了分析，并与S-RNA图谱进行了比较，两者图谱中光吸收点的分布一致，相应点经洗脱后，由光谱分析与核碱组成测定证明均含有相同的核苷酸组成。
In chapter two, it was studied in detail how to enhance the RNase production.
第二章详细研究了如何提高黑曲霉的产酶水平。
These results suggest that human RNase 9 may play roles in host defense of male reproductive tract.
这些结果表明RNase 9蛋白可能在男性生殖道的宿主防御中发挥重要作用。