The characterization of the fermental Toxigenic Pasteurella multocida (Pm 13-1) is studied according to the vaccine standard.
为检验该培养方法培养出的T+Pm是否符合对T+Pm制苗用菌液的生物学特性鉴定标准，分别取样作培养特性检验、菌体形态检验、糖发酵实验、T+Pm毒素产生的检测及荚膜型鉴定。
The study is to improve the producing efficacy of AR vaccine. Toxigenic Pasteurella multocida.(Pm 13-1) was cultured in the 15L BIOSTAT. C fermentor with experimental parameters (Time, Air Flow, Speed of stir, and pH).
为提高猪传染性萎缩性鼻炎疫苗生产效率，采用工作容积15立升的BIOSTAT·C型发酵罐，培养经鉴定作为制苗菌株的产毒素多杀巴氏杆菌Pm 13-1株。
This method could amplify the specific fragment in the reference strains, but not in chicken E. coli, Haemophilus paragallinarum, Salmonella Pullorum and Avian pasteurella multocida, of ORT. A sensitivity test indicated that the assay's lowest detection limit was 90 pg DNA.
该方法能在ORT参考菌株中扩增到特异性片段，而鸡大肠杆菌、副鸡嗜血杆菌、鸡白痢沙门氏菌、禽多杀性巴氏杆菌的扩增结果均为阴性；敏感性试验表明该方法最低检出限量为90 pg DNA。
This study was aimed to construct and characterize Salmonella vaccine strain C500 expressing the recombinant Pasteurella multocida toxin antigen by the Asd+ balanced-lethal host-vector system.
本研究利用Asd+平衡致死系统构建表达巴氏杆菌毒素(Pasteurella multocida toxin，PMT)的重组猪霍乱沙门氏菌株，并对重组菌株的生物学特性进行比较研究。
The indirect IS-PCR main conditions were optimized as follows: tissue sections were treated by 0.2 mol/L HCl at 37℃ for 20 minutes, by 100 μgml^(-1) protease K at 37℃ for 15 minutes; The working condition of probe and Avidin-AP of ISH were 350 ngml^(-1) and 1:100, respectively. The positive results were gained by indirect IS-PCR for detecting the duck liver paraffin sections infected by DEV, but negative results to the duck liver paraffin sections of dead ducks infected with duck viral hepatitis virus, Riemerella anatipestifer, Pasteurella multocida, Salmonella typhimurium, E. coli (O78). The positive signals were detected from hepatocyte, sinusoidal endothelial cell and Kupffer's cell of duck liver infected artificially with DEV by indirect IS-PCR at 2, 4, 6, 8, 12, 24, 48 and 72 h, respectively. Most of them appeared in debris of cellular necrosis or vacuole formed in infected cells.
间接原位PCR对DVE死亡鸭肝脏的石蜡标本检测结果为阳性，而鸭病毒性肝炎、鸭疫里默氏杆菌病、鸭多杀性巴氏杆菌病、鸭沙门氏菌病和鸭大肠杆菌病死亡鸭肝脏的石蜡标本检测结果为阴性；间接原位PCR对人工感染DEV后2、4、6、12、24、48和72 h不同时间的鸭肝脏检测结果均为阳性，阳性细胞有肝细胞、窦皮细胞和枯否氏细胞，阳性信号多出现于坏死细胞的碎片中或细胞坏死后形成的空泡内及空泡边缘；对存档蜡块、临床病料的检测与病毒分离鉴定吻合率为100%。
Pasteurella multocida is an important pathogen that infects many kinds of animals.
多杀巴斯德氏菌是养殖动物的重要致病菌。
In conclusion, 12 avian specific monoclonal antibodies against Pasteurella multocida were obtained in this study. Certain of them provided good passive protection in mice model.
总结实验，以ATCC 12945免疫小鼠后所生产之单株抗体，在抗体特性分析试验中，发现单株抗体能专一的辨认部分巴斯德杆菌之禽分离株。
With the defined the parameters, the concentration of Toxigenic Pasteurella multocida (Pm 13-1) in the fermentor could reach 45~56 billion/ml, over 6-7 times higher than that of the traditional culture.
将各适宜条件优化组合，Pm 13-1株发酵培养细菌浓度可达450—460亿/毫升，是过去玻璃瓶通气培养细菌浓度的7—8倍。
Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.
扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。
The minimal bactericidal concentration of 40% EtOH extractum for Escherichia coli was 6.25mg/mL, for Staphylococcus aureus, Salmonella and Pasteurella multocida was 12.25 mg/mL, for Streptococcus agalactia was 25.00mg/mL.
对金黄色葡萄球菌、沙门氏菌和多杀性巴氏杆菌的MBC均为12.25mg/mL，对无乳链球菌的MBC为25.00mg/mL。
Tulathromycin possesses in vitro activity against mannheimia haemolytica, pasteurella multocida, histophilus somni and mycoplasma bovis, and actinobacillus pleuropneumoniae, pasteurella multocida and mycoplasma hyopneumoniae, the bacterial pathogens most commonly associated with bovine and swine respiratory disease, respectively. increased MIC values have been found in some isolates of histophilus somni and actinobacillus pleuropneumoniae.
泰拉菌素拥有的体外抗菌活性对溶血性曼氏杆菌，巴氏杆菌，支原体，胸膜肺炎放线杆菌，多杀性巴氏杆菌和猪肺炎支原体，常见的病原体与牛和猪呼吸系统疾病有效。
At present it is widely accepted that the damages of the nasal turbinate bones and mucus caused by Bordetella bronchiseptica laid the foundation for the invasion toxigenic Pasteurella multocida. The mixture of Bordetella bronchiseptica and toxigenic Pasteurella multocida, together with some other factors, is the main cause of natural AR.
经过多年研究，现在大多数学者认为，支气管败血波氏杆菌能引起一定程度的鼻粘膜和鼻甲骨损害，但这些病变的严重后果是为正常猪鼻腔不能定居的产毒素多杀巴氏杆菌建立感染铺平道路，Bb和T+Pm的混合感染加上非传染因素交织在一起，是在自然条件下临床型AR的发生原因。
Establishment of a Multiplex-PCR for Detecting Actinobacillus pleuropneumoniae, Pasteurella multocida and Haemophilus parasuis
猪的胸膜肺炎放线杆菌、多杀性巴氏杆菌和副猪嗜血杆菌复合PCR检测方法的建立
Study on the Method of a Latex Agglutination Test Detecting Antibodies Against Pasteurella Multocida Toxin; Accelerate circulation of blood, clear away toxin and swell.
检测产毒多杀性巴氏杆菌毒素抗体乳胶凝集试验方法的研究促进血液和淋巴循环，排毒、排水、消肿。
Studies on serotyping of Pasteurella multocida isolated from fowl cholera in Heilongjiang Province (first report)
黑龙江省鸡霍乱多杀性巴氏杆菌血清学分型的研究（第一报）
Fowl cholera is an acute, highly contagious and septicemic disease caused by Pasteurella multocida (Pasteurella multocida, P. m) strains.
禽霍乱是由禽多杀性巴氏杆菌（Pasteurellamultocida，P.m）引起的在鸡、鸭等禽类中发生的一种急性、接触性、败血性传染病。
STUDIES ON THE CAPSULAR SUBUNIT VACCINE AGAINST FOWL CHOLERA & ⅲ. Some of Physical and Chemical Properties of the Capsula Antigen of Pasteurella Multocida
禽霍乱荚膜亚单位菌苗的研究&Ⅲ、禽多杀性巴氏杆菌胞壁荚膜抗原的若干理化特性
Progress of Research on the Toxigenic Pasteurella Multocida of Porcine Atrophic Rhinitis
猪萎缩性鼻炎产毒多杀性巴氏杆菌的研究进展
Our laboratory study showed that the plpE gene of Multocida Pasteurella has a high conservative. Literature reported both at home and abroad that the PlpE protein may be a cross protective antigen.
本实验室有相关研究表明，多杀性巴氏杆菌的plpE基因具有较高的保守性；并且，国内外均有文献报道，多杀性巴氏杆菌的PlpE蛋白很可能是一种交叉保护性抗原。
Epidemiological studies on toxigenic Pasteurella multocida (Pm) and Bordetella bronchiseptica (Bb) in infectious atrophic rhinitis (AR) in swine
产毒素多杀巴氏杆菌和支气管败血波氏杆菌在猪萎缩性鼻炎中的流行病学研究