Homology analysis showed that the sequence of P32 gene shared 99% and 97% homology with that of goat capripoxvirus and sheep capripoxvirus which have already published, and the homology of deduced amino acid sequence shared 98.9% and 96%, respectively.?
与山羊痘病毒和绵羊痘病毒P32基因序列同源性分别达99 %和97 %；与山羊痘病毒和绵羊痘病毒P32基因编码的氨基酸同源性分别达98.9 %和96 %。
The results of sequencing show that GTPV P32 gene was 969bp and encode 323 amino acids, Sheep pox virus P32 gene was 972bp and encode 324 amino acids, FGD-CPV P32 gene was 969bp and encode 323 amino acids.
结果表明：GTPV P32基因开放阅读框全长969bp，编码323个氨基酸；绵羊痘病毒(Sheep pox virus，SPPV)P32基因ORF全长972bp，编码324个氨基酸；羊胎皮肤细胞分离毒P32基因ORF全长969bp，编码323个氨基酸。
Capripox, caused by capripoxvirus, is an acute feverish and contagious disease in sheep or goat. Capripox is classfied as group A diseases by Office International des Epizootiesand group first diseases in China. P32 protein is one of the virus structure proteins.
羊痘是由羊痘病毒属病毒引起的绵羊或山羊的一种急性、热性、接触性传染病，被世界动物卫生组织列为A类传染病，在我国被列为一类传染病。
The structural protein P32 gene of goat capripoxvirus was amplified by PCR method using specific primers. The P32 gene was ligated with pMD18?
应用PCR技术，用特异性引物扩增出羊痘病毒结构蛋白P32基因，连接于pMD18？
Two pairs of specific primers were designed and synthesized according to the inverted terminal repeat sequence and p32 gene sequence of the genome of capripoxvirus.
根据羊痘病毒末端反向重复序列中的一段序列和P32基因序列，设计合成了2对引物，通过确定最佳的PCR反应条件，建立了检测羊痘病毒核酸的多重PCR方法。
The P32 gene of goatpox virus was amplified by PCR, and then cloned into a baculovirus expression vector pFastBacⅠ. A recombinant plasmid pFastBac-P32 was constructed and then transformed into DH10Bac competent cells. White colonies of the recombinant Bacmid-P32 were obtained by screening with three antibiotics and selection of blue-white patches. After the Bacmid-P32 was transfected into Sf9 insect cells, the recombinant baculovirus was obtained by three passages. Finally, the expressed product from the P32 gene was detected by SDS-PAGE and Western-blotting.
采用PCR亚克隆了山羊痘病毒P32基因片段，将其插入杆状病毒转座载体质粒pFast-BacⅠ中，构建了重组质粒pFastBac-P32；再将该质粒转化DH10 Bac感受态细菌，进行体内重组，然后经三重抗性及蓝白斑筛选，获得杆状病毒重组质粒Bacmid-P32；将Bacmid-P32转染Sf9细胞，获得重组杆状病毒；最后用SDS-PAGE和Western-blotting对P32基因的表达进行检测。
Scientists are hopeful that radioactive chromic phosphate, p32 can be used as an effective mesothelioma treatment.
科学家们希望，放射性磷酸铬，p32可以作为一种有效的治疗间皮瘤。
This method conformed the following conditions: spleen phosphodiesterase and micrococcal nulcease for digesting reagent and the concentration, temperature and digesting time of the two enzymes; the components of p32-post label alleviant and labeling time; the concentration of p1 nuclease; the concentration of T4 polynucleotide kinase for catalysing and labeling reagent; the components of bidirectional spreader and the spreading time, etc.
确定了采用脾磷酸二酯酶和微球菌核酸酶消化底物以及两种酶的使用浓度、温度及消化时间；确定了P32标记缓冲液的主要成分及标记时间；确定了P1核酸酶的浓度；确定了T4多核苷酸激酶催化标记底物的浓度；确定了双向展开剂的主要成分及展开时间等。
We advanced the theory of ultra-narrow bandwidth optical filter consists of two-dimensional (PCADOF) photonic crystal composed of anomalous dispersion material cylinders set on dielectric medium with the square lattice.
本文讨论了利用由反常色散材料构成的二维（2D）光子晶体实现超窄带滤波的原理，并分析了铯原子蒸汽6P32？
Cloning, Sequencing and Expression of Goat Poxvirus P32 Gene
山羊痘病毒P32基因克隆、测序及表达
Construction and expression of recombinant antigen P32 gene of goat poxvirus
山羊痘病毒P32基因真核表达载体的构建及表达
The medium for cultivation of fluorescent pseudomonads strain P32 was studied. According to the tests, the optimum medium for its growth consists of sugar 6%, glycerol 3%, peptone 1%, (NH_4) H_2PO_40.5%.
本文对荧光假单胞菌P32的培养基成分进行了研究，利用正交试验筛选出产菌量较高的培养基，成分如下：（%，W/V）蔗糖6，甘油3，蛋白胨1，NH4H2PO4,0.5。
Studies on Some Biological Characteristics and P32 Gene Encoding for the Main Structural Protein of Goat Pox Virus
山羊痘病毒某些生物学特性及其主要结构蛋白P32基因的研究
Expression of P32 gene of goatpox virus in baculovirus
山羊痘病毒P32基因在杆状病毒中的表达
Cloning and sequence analysis of P32 surface protein genes of three strains of bovine Theileria sergenti
三株牛瑟氏泰勒虫P32表面蛋白基因的克隆与序列分析
Cloning of the Major Surface Protein P32 Gene from Ovine Theileria and Construction of Eukaryotic Expression Vector
羊泰勒虫主要表面蛋白P32基因的克隆及真核表达载体的构建
P32 protein is one of the virus structure proteins. It stands out on the virus envelope surface and contains neutralized antigen epitope.
羊痘病毒P32蛋白是位于羊痘病毒膜表面的主要结构蛋白，含有中和抗原表位；
Finally, the expressed product from the P32 gene was detected by SDS-PAGE and Western-blotting.
最后用SDS-PAGE和Western-blotting对P32基因的表达进行检测。
The results showed that P32 and CD58 were successfully expressed in BHK-21 cells.
结果显示，P32和CD58在BHK-21细胞中都得到表达。
Establishment of PCR method and sequence analysis for P32 gene: According to the sequences of P32 gene in GenBank, a pair of specific primers were designed, and a PCR method was established successfully.
基于P32基因PCR方法的建立及其基因序列分析：参照GenBank已发表的GPV基因序列，针对P32基因设计与合成了1对特异性引物，成功地建立了针对P32基因的PCR方法。