METHODS Human IL 6 gene was reconstructed in retrovirus vector and transferred into incasing cells PA317 by lipofectamine mediated method. The clones of the cells transferred with hIL 6 were selected by G418. Targeted NIH3T3 cells were infected with the virus granules secreted from PA317 and also selected by G418. The insertion and expression of hIL 6 gene in NIH3T3 cells were analysed with Southern blot and Northern blot. RESULTS Human IL 6 retrovirus vector (pZIPIL-6) was successfully reconstructed.
利用重组载体构建技术将质粒pUCIL 6 cDNA的目的片段连接于逆转录病毒载体上，并以脂质体介导的方法将重组载体转染包装细胞PA317，以G418筛选克隆细胞，浓缩克隆细胞上清以制备重组病毒液，继之感染NIH3T3细胞后，进行Southern blot和Northern blot分析，检测目的基因在靶细胞的整合与转录水平。
The effects of heat stress on HSP70 gene expression, energy supply in abscission zone of flower-pod and the abscission rate of flowers-pods in soybean cultivars were analyzed by Northern blot and physiological and biochemical assay.
实验运用Northern blot检测和生理生化分析等技术研究热胁迫对大豆品种花荚离层细胞的HSP70基因表达、能量供应及花荚脱落的影响。
In this paper, a deleted mutation in RNA2 of WYMV was detected after the 27 passage of mechanical innoculation in wheat by RT-PCR and Northern blot. Sequence analysis showed that a contiguous sequence of 2595 nucleotides (from nt 214 to nt 2808) was internally deleted from WYMV RNA2 and flanked by seven reverse repeated compatible nucleotides which can form a loop-stem structure.
利用在小麦上连续机械接种WYMV的方法，自第27代起，利用RT-PCR和Northern blot方法在感病小麦中检测到一个WYMV RNA2的缺失突变株，序列分析发现缺失区域位于RNA2的214~2808nt，共计2595nt，并在缺失区域的两端存在7个碱基的反向互补序列。
In this paper, a deleted mutation in RNA2 of WYMV was detected after the twenty-seventh passage of mechanical inoculation in wheat by RT-PCR and Northern blot. Sequence analysis showed that a contiguous sequence of 2595 nucleotides (from nt 214 to nt 2808) was internally deleted from WYMV RNA2 and flanked by seven reverse repeated compatible nucleotides which can form a loop-stem structure.
利用在小麦上连续机械接种WYMV的方法，自第27代起，利用RT-PCR和Northern blot方法在感病小麦中检测到一个WYMV RNA2的缺失突变株，序列分析发现缺失区域位于RNA2 的214-2808nt，共计2595 nt，并在缺失区域的两端存在7个碱基的反向互补序列。
The more accurate localization of β3GalT-l mRNA expression in mouse brain as studied by in situ hybridization was in good agreement with the general expression pattern seen with Northern blot hybridization.
由于p4Ga1T一2在成年小鼠皋丸中表达最高，我们进一步用原位杂交分析了其在生精小管内的表达定位。
Northern blot demonstrated that the expression of UidA gene and endog enous GluA-2 gene reached their highest level at 13 ～15 days and 11～13 days after pollination respectively, and then declined.
Northern blot检测表明，开花后13～15 d和11～13 d，UidA基因和水稻内源的GluA-2 基因的表达量分别达到最高，随后逐渐降低。
Methods (1) Northern Blot was conducted to determine the change of gene expression.
（1） Northern blot测定2810430M08Rik mRNA的表达水平。
METHODS:Northern blot hybridization and immunohistochemical technique.
采用Northern核酸分子杂交和免疫组化技术。
Northern blot and RT-PCR analysis demonstrated a ubiquitous pattern of gene expression in human and mouse tissues.
运用Northern杂交和RT-PCR方法检测发现UBAP1基因在所检测的人和小鼠的组织中广泛表达。
Northern blot analysis was used to test the expression of ARF1 gene in cardiac hypertrophic rats.
4通过Northern印迹杂交的方法检测ARF1在心肌肥厚大鼠中的表达变化。
The rtSH3p13 gene is expressed in the testis and brain, based on Northern blot analysis of various tissues.
本组前期的工作对rtSH3p13基因进行了初步的研究。
Methods bFGF concentrations were determined by ELISA. Northern blot hybridization was performed to detect the expression of rnRNA.
ELISA法检测防GF的蛋白表达水平；mRNA的水平用Narthern印迹杂交法研究。
Here, the expression of these genes induced by tapping, mechanical wounding and exogenous jasmonates was analyzed by RT-PCR and Northern-blot. The results as follows:(1) The expression of genes encoding for chitinase, β-1, 3-glucanase and hevein was up-regulated by mechanical wounding and exogenous jasmonates, with the effect of the former being lagged behind that of the later; (2) The expression of these genes is remarkably stronger in tapping trees than that in virgin trees; (3) The level of endogenous jasmonates in the latex of tapping trees is significantly higher than that in virgin trees.
本文采用RT-PCR和Northern-blot技术分析割胶、机械伤害和外茉莉酸对胶乳几丁质酶、β-1，3-葡聚糖酶和橡胶素基因表达的影响，获得如下结果：（1）外源茉莉酸和机械伤害均能上调几丁质酶、β-1，3-葡聚糖酶和橡胶素的基因表达，但机械伤害的效应滞后于外源茉莉酸；（2）几丁质酶、β-1，3-葡聚糖酶和橡胶素的基因在割胶树中的表达明显强于在未开割树中的表达；（3）割胶树胶乳中内源茉莉酸含量显著高于未开割树。
OsBP-5 mRNA was detectable in developing rice seed as well as in root and leaf by Northern blot assay.
用Northern印迹的方法检测到OsBP-5基因在水稻的叶、根和未成熟种子中均有表达。
Analysis of multiple fragments of single slot blot, and requirement of RNA only in small amounts as compared to conventional Northern makes the protocol quick, effective, and economic.
通过对传统方法的改进，大大提高了工作效率，降低了实验成本和工作量并对相关问题进行了探讨，为进一步的研究奠定了基础。
We have had a series of experiments on stresses on Wild-type tomato, rin, Nr(Never-ripening, Nr) and T4B-11(ACC oxidase I co-suppression transgene tomato, T4B-11), collected correlative samples to extract total RNA, and amplified gene fragment of PR-1, PR-5 and PR-NP24. Then these genes were analyzed by Northern blot hybridisation technology in mRNA level.
本文对普通番茄(Wild-type，WT)，番茄乙烯生成和果实成熟均受到抑制的番茄突变体(ripening inhibitor，rin)，乙烯不敏感型突变体(Never-ripening，Nr)以及1-氨基环丙烷羧酸氧化酶Ⅰ协同抑制(co-suppression)的转基因番茄T4B-11进行了一系列胁迫条件处理，并且收集样品提取总RNA，通过PCR或RT-PCR克隆番茄中几个病程相关蛋白PR-1，PR-5，PR-NP24的基因片段作为探针来进行Northern杂交实验，在mRNA水平上研究了这些基因的表达模式。
Sialyltransferase differentially expressed in different cancer cell lines. The size of the transcriptional product varied from 8.0Kb to 8.5Kb in cancer cell lines.
Northern Blot 杂交分析表明，人胎肝ST3Gal I唾液转移酶基因在不同的癌细胞系中表达不同，表达产物约为8.0-8.5Kb。
Northern blot analysis and immunocytochemistry techniques were performed to detect the expression of typeⅠ, Ⅲ procollagen mRNA and their relevant proteins in cultured rat pulmonary fibrobasts after FN treatment.
用免疫组织化学方法观察实验性大鼠肺纤维化纤维连接蛋白的动态变化；用 Northern印迹杂交和免疫细胞化学方法分别观察FN对体外培养的大鼠肺成纤维细胞Ⅰ、Ⅲ型前胶原mRNA和蛋白表达的影响。
Northern blot analysis showed that the expressions of G14 and G15 were neither distinctly different between S45A and S45B.
Northern blot分析表明G14和G15的表达量在S45A和S45B之间没有明显差异。
Northern blot and RT-PCR give positive signal specifically in ovary tissus, the full length of spt1 is about 4.5-5.0 kb Yeast transformation and sequence truncation experiment suggested that spt1 could mediate the secretion of invertase.
Northern blot 和RT-PCR分析表明，该序列仅表达于小鼠卵巢组织，全长约4.5～5.0 kb。酵母转化和序列截短实验提示，该序列能够介导蔗糖转换酶向细胞外的分泌。