Synfuels China also planned a huge CTL plant in Guizhou Province and prepared a pre-feasibility study on the project.
中科合成油在贵州也规划了煤制油项目，目前已经完成了预可研。
Granzyme B is member of serine proteinase family and plays an essential role in CTL/NK-mediated killing.
granzyme B是丝氨酸蛋白酶家族的成员，是CTL和NK细胞产生的主要杀伤性分子。
The cytotoxity of inducing CTLs was higher than that in the control group.
诱导的CTL体外杀伤活性高于对照组。
Li said Yitai CTL project is currently under modification including safety and fire-fighting certification formalities etc, and scheduled to restart up by the end of July and enter long period operation in early August, to reach full load production and keep running for the whole winter.
据李永旺介绍，目前伊泰煤制油装置正在进行整改，办理安检、消防等手续，预计将于7月底进行第二次试车，8月初正式进入试生产长期运行，实现满负荷生产，并在运行中过冬。
Recombinant plasmids of pCMV-CTB-AWTE were given to five rhesus monkeys three times with two weeks intervals by intramuscle route, immunization dose was 500ng per plasmid per animal. Results: High levels of anti-CTB and anti-malaria epitopes antibodies and P. falciparum epitope specific CTL activity were elicited.
结果表明：DNA疫苗免疫恒河猴后，用1.25×108个食蟹疟子孢子攻击，对照组5只动物在攻击后14天左右全部感染，感染持续34天以上；DAN疫苗组的5只动物一直到攻击后60天，没有感染。
Notably, successful CTL induction was identified in all of these patients.
值得注意的是CTL诱导的成功在这些患者中得到验证。
Aim To study the effect of diterpenoids of Andrographis paniculata on CTL activity in mice sensibilized by B16 cells.
目的 研究穿心莲二萜类化合物对B16细胞致敏小鼠脾脏CTL杀伤活性的影响。
Results The siRNA targeting human VEGF effectively silenced VEGF gene and inhibited the secretion of VEGF in MCF-7, whereas the control scramble siRNA showed no effects; MCF-7 cell supernatant could inhibit the differentiation, maturation and function of DC induced from PBMC, the cultured DC cultured with MCF-7 cell supernatant showed low expression of CD80, CD83, CD86 and HLA-DR, and IL-12 secreted by DC and IFN-γproduced by PBMNC all reduced(p＜0.01After silenced VEGF gene DCs cultured in the culture supernatants from MCF-7 cells had significantly higher expression of CD86, CD80, CD83 and HLA-DR than the control group(p＜0.01), IL-12 secreted by DCs and IFN-γproduced by PBMNC were improved significantly(p＜0.01The activity of antitumor of dendritic cells loaded tumor antigen combinated siRNA targeting VEGF gene was markly, the target cells were lysised, and the inhibition rate was 99%.
结果设计合成的siRNA转染MCF-7细胞后VEGF mRNA和蛋白表达水平明显减低，同时抑制了靶细胞的增殖；在体外应用粒-巨细胞集落刺激因子，白介素-4(IL-4)及肿瘤坏死因子α能有效培养人外周血单个核细胞来源的DC；MCF-7乳腺癌细胞培养上清能够明显抑制正常外周血单个核细胞诱导的树突状细胞的分化成熟及抗原提呈能力，CD80、CD83、CD86和HLA-DR的表达明显降低(p＜0.01)，DC致敏的CTL对MCF-7细胞杀伤活性及IL-12分泌和共刺激T淋巴细胞所分泌的IFN-γ明显降低(p＜0.01)；干扰VEGF基因后MCF-7乳腺癌细胞培养上清对DCs的影响明显降低，CD80、CD83、CD86和HLA-DR的表达显著升高，DC致敏的CTL对MCF-7细胞杀伤活性及IL-12分泌和共刺激T淋巴细胞所分泌的IFN-γ明显升高(p＜0.01)；靶向VEGF基因的siRNA和DC联合应用可显著抑制肿瘤细胞生长，细胞几乎完全溶解，细胞杀伤率达99%。
DCs acquired by our reformed methods express both CD83 and CD 14 molecules highly, and have a higher density than other domestic reports. The higher TNF in DCs culture medium of HC patient suggests DCs in patient still have antigen presenting ability and by optimization the culture medium would improve its presenting ability and have a potential value in design and application individual vaccine. Although antigens pulsed DCs have a decrescent antigen presenting ability but BCG HSP70 could induce its mature and improve its presenting ability. Suggests BCG HSP70 would be a useful mature inducer. Lymphocytes primed by DCs based HC vaccine have the specific cytotoxicity against HCC lines. The CTL after freezing and anabiotic could prophylaxis and therapy HC xenograft on nude mouse. The results also suggests that CD4〓 lymphocytes play a important role in HC with a good differentiation and would be useful in treatment this kind of HC. After being activated by Peptide LLNQHACAV of hAFP and apoptotic HCCs pulsed DCs respectively, the culture medium of activated lymphocytes both contains a high level Th1 cytokines IL-12 and TNF. Primed lymphocytes appeared a characteristic of NK cells. DCs not only inhibited the growth of human HCC and other cancer cells in vitro but also prevented the growth of HC xenograft on nude mouse in vivo. There are at least three kinds of mechanism playing important role in DC based vaccine, there are inhibition of DCs, HC specific CTL and cytokines pathway.
诱导出的DC共同表达CD83和CD14分子，CD83分子表达明显高于国内报道；肝癌患者DC培养上清中TNF水平高于健康人，提示肝癌患者DC仍具一定的抗原呈递能力，适当调控可使其行使APC功能，以期在肝癌个体化疫苗中发挥作用；DC负载肝癌可溶性抗原后，抗原呈递能力降低，BCG HSP70可促进DC成熟，增加其抗原呈递能力，预示BCG HSP70有可能成为促进DC活化和成熟的另一重要分子；肝癌DC疫苗在体外诱导肝癌特异性淋巴细胞，活化的淋巴细胞在体外对肝癌细胞的杀伤以特异性CTL为主，同时分泌较高水平Th1型细胞因子IL-12和TNF，并抑制4种肝癌细胞生长；冷冻复苏后的肝癌特异性淋巴细胞可以预防和抑制人肝癌裸鼠皮下移植瘤，提示DC负载肝癌可溶性抗原后诱发的MHC-Ⅱ类限制性CD4〓T细胞有可能在分化程度高的肝癌治疗中发挥作用；用DC和HLA-A2〓DC分别负载凋亡肝癌细胞和hAFP218-226位LLNQHACAV HLA-A2限制性九肽，在体外诱导肝癌特异性淋巴细胞，活化后的CTL细胞分泌较高水平的Th1型细胞因子IL-12和TNF，并具较强杀伤活性，此CTL同时具备NK细胞特征；DC对肿瘤细胞的抑制作用可能是通过吞噬实现的，Fas-L在DC抑制中也起一定作用；DC对人肝癌裸鼠皮下移植瘤的抑制率为97％；在肝癌DC疫苗的作用中，至少联合3种以上机制，即通过DC的直接作用、肝癌特异性CTL和细胞因子途径直接或间接地杀伤和抑制肝癌细胞。
For example, using antiseptic such as ACQ, CCA, AAC, CDDC, CTL, IPBC and so on to lengthen service life lignin material.
二是利用防腐、防虫、防霉的化学试剂来延长木质材料的使用寿命；例如，利用ACQ、CCA、AAC、CDDC、CTL、IPBC等防腐剂来延长木质材料的使用年限。
The killing effect to K562 cells of CTL actiVated by different exosomes is stronger than Kexo. The killing effect of CTL activated by exosomes of DCs derived from K562 cells、hot shork K562 cells、DCs derived from K562 cells activated by total RNA or freeze thawing antigen of K562 cells is stronger than CTL activated by exosomes derived from K562 cells.
Pexo诱导的CTL对自体白血病细胞的杀伤作用对CML患者原代白血病细胞来源exosomes诱导的CTL对自体白血病细胞的杀伤作用的研究显示：Pexo的免疫原性和Kexo有所不同，Pexo诱导的CTL对患者白血病细胞的杀伤作用比Kexo诱导的更强。
Although these class I HLA derived peptides functioned, at least in part, by inhibiting CTL and NK cells, their mechanism of action was incompletely understood.
亲和层析法研究表明，HLA衍生肽的作用机制可能与血红素氧化酶-1(heme oxygenase -1，HO-1)有关。本研究采用计算机辅助分子设计理论，在HLAⅠ类衍生肽B2702.75-84的基础上设计出一种新型的HLA衍生肽，RDP1258。
QS-21, an acylated bidesmosidic triterpenoid saponin isolated byKensil et al in 1992 from the South American tree, Quillaja Saponaria Molina, is a potentimmunological adjuvant It has been shown highly effective in inducing CTL and antigen-specificantibody responses to exogenously added antigens and enhancing both humoral and cell-mediatedimmune responses.
QS-2l是1992年由美国化学家Kensil等人从蔷薇科植物QuillajaSaponria Molina中分离得到的一种带脂肪链的双糖链三萜皂甙，具有极强的免疫辅助活性，能有效诱导外加抗原的胞毒T淋巴细胞的响应及抗原专一性抗体的产生，并能增强细胞的免疫反应。
Methods: Mononuclear cells were isolated from human peripheral blood and cultured for 7 days in medium with GM-CSF and IL-4 to get dendritic cells; Tca-8113 cells were cultured with RPIM1640, the tumor-derived exosomes were isolated and purified from the culture supernatants by ultrafiltration with Millipore centrifugal Filter Devices; Then exosomes was pulsed onto DC, the DCs were cocultured with the peripheral blood lymphocytes to transform T cell into specific CTL.
从外周静脉血中分离和培养树突细胞，分离纯化舌鳞癌Tca-8113细胞培养上清液中的exosome，并将exosome负载至DC上，诱导T淋巴细胞活化为细胞毒性T细胞，把CTL加入到靶细胞（Tea-8113和95-D）培养体系中，18h后利用MTT比色法测定CTL对靶细胞的杀伤活性。
When the TC1232COA detects that the LPS input is inactive, the PHY-LLC interface is placed into a low-power reset state in which the CTL and D outputs are held in the logic 0 state and the LREQ input is ignored; however, the PCLK output remains active.
当检测到TC1232COA脂多糖输入无效时，物理层，LLC的接口被放置到一个低功耗复位状态，其中的CTL和D产出的逻辑举行0国家和LREQ输入将被忽略，但是，PCLK时钟输出保持活跃。
H22 tumor cells derived gp96 peptide complexes can induce CTL response of spleen lymphocyte of mouse in virto; Besides, those CTLs show the specific antitumor effect to H22 tumor cells and screat the immunologic active material to induce apoptosis of H22 tumor cells.
肿瘤来源的热休克蛋白gp96肽复合物能诱导小鼠脾淋巴细胞的CTL反应，该活化的CTL具有特异性抗H22肿瘤细胞的免疫作用，并能分泌免疫活性物质诱导H22肿瘤细胞凋亡。
AIM: To realize high cell? ?? ? density fermentation of MAGE3/HSP70 engineered E. coli, and to evaluate the antitumor immunity of MAGE3/HSP70 fusion protein in vivo. METHODS: High cell? ?? ? density fermentation of MAGE3/HSP70 enginered E. coli was carried out with constant soluble oxygen and continuous cultivation in self? ?? ? control fermentor.
assosiated gene，MAGE）是第一类被发现的肿瘤排斥抗原［1-2］，MAGE3是MAGE家族成员之一，不仅在黑色素瘤表达，同时在其它多种肿瘤中均有不同程度的表达，能够诱导机体特异性的CTL反应，更重要的是在除睾丸和胎盘外的正常组织中均不表达，人们认为它是肿瘤特异性免疫治疗理想的靶分子之一［3-4］。
AIM: To realize high celldensity fermentation of MAGE3/HSP70 engineered E. coli, and to evaluate the antitumor immunity of MAGE3/HSP70 fusion protein in vivo. METHODS: High celldensity fermentation of MAGE3/HSP70 enginered E. coli was carried out with constant soluble oxygen and continuous cultivation in selfcontrol fermentor.
黑色素瘤抗原（melanoma antigenassosiated gene，MAGE）是第一类被发现的肿瘤排斥抗原［1-2］，MAGE3是MAGE家族成员之一，不仅在黑色素瘤表达，同时在其它多种肿瘤中均有不同程度的表达，能够诱导机体特异性的CTL反应，更重要的是在除睾丸和胎盘外的正常组织中均不表达，人们认为它是肿瘤特异性免疫治疗理想的靶分子之一［3-4］。
After identification, they can be used in the designs and studies of tumortherapeutic peptide vaccine.
所预测出的6个HLA-A2限制性CTL表位经后续实验筛选、鉴定后，可用于基于MAGE-3的肿瘤治疗性多肽疫苗的设计研究。
Results The susceptibility of CaSki cell to CTL lysis under the stimulation of rSIFN-co was better than Infergen, IFNα-2b and DDP induced. The expression of CD54 and CD40 on cervical cancer cell was also increased. And this effect had positive correlation to the drug concentration.
结果 经重组高效复合干扰素诱导后的CaSki细胞对CTL杀伤作用的敏感性高于经另两种干扰素及顺铂诱导的CaSki细胞，这种效应与CaSki细胞表面黏附分子CD54及CD40的表达强度呈正相关，表现出剂量－效应关系。