The templet DNA was separated from scabs of wild CPV infected goat and sheep, and also from cultures of CPV vaccine infected fetal goat dermal cells. DNA fragments nearly 1kb were amplified by PCR, and they were inserted into vector pMD18T and sequenced.
从感染羊痘的山羊和绵羊痂皮以及山羊痘病毒疫苗感染的羊胎皮肤细胞培养物中提取DNA，利用PCR方法分别扩增出大约1kb的DNA片段，将其插入克隆载体pMD18-T并测序。
Capripoxvirus can cause the sheeppox, goatpox and lumpy skin disease.
羊痘病毒(Capripoxvirus，CPV)能分别引起山羊痘、绵羊痘和牛的结节性疹块病。
The results of sequencing show that GTPV P32 gene was 969bp and encode 323 amino acids, Sheep pox virus P32 gene was 972bp and encode 324 amino acids, FGD-CPV P32 gene was 969bp and encode 323 amino acids.
结果表明：GTPV P32基因开放阅读框全长969bp，编码323个氨基酸；绵羊痘病毒(Sheep pox virus，SPPV)P32基因ORF全长972bp，编码324个氨基酸；羊胎皮肤细胞分离毒P32基因ORF全长969bp，编码323个氨基酸。
With the guidance of the theory of dose attraction and dose repulsion introduced by Professor Shuxiang Li, we established the model of relative dose distribution in beam rotation plane by deeply analyzing the character of dose distribution. We also introduced the calculation formulas of dose attraction and dose repulsion, and improved the calculation of CPV and BPV.
在简要介绍了李树祥教授提出的剂量引力和剂量斥力思想之后，以此为指导，通过深入分析照射面的剂量分布特点，建立了照射面相对剂量分布的数学模型，提出了剂量引力和剂量斥力函数的计算公式，以及利用CPV、BPV自动选择最优照射弧的排队择优算法。
During restoration experiment, several functional and structural parameters, species, percentage of phytomastigophora, CPV, diversity index, heterotro-phy index and similarity index, were determined weekly.
生态治理过程中水生生物群落的恢复，从理论上讲首先是微型生物群落，然后才是浮游动物、底栖动物和鱼类。因为生态系统的演替，应是从r一对策占主导逐渐发展到k一对策占主导。
Finally, we immunized BALB/c mice with the same dose of the purified protein VP2S or CTLA-4-VP2S and compared their antibody responses by enzyme-linked immunosorbant assay and haemagglutination inhibition assay. After 30 cycles of amplification, agarose gel electrophoresis revealed expected PCR products for both gene fragments. Sequence analysis showed that the amplified extracellular domain was 99.2% identical to previously published sequence without mutation in the hexapeptide motif for B7 molecule binding. After IPTG induction, the vector-transformed E. coli expressed expected 29-kDa VP2S protein and 42-kDa CTLA-4-VP2S protein. Western blotting showed that both VP2S and CTLA-4-VP2S proteins were recognized by CPV-specific antiserum. After two times of immunizations, the VP2S-specific antibody appeared from week 2 and reached the highest level at week 4 in CTLA-4-VP2S-immunized mice. In VP2S-immunized mice, however, the specific antibody appeared from week 4 and reached the highest level at week 5. The CTLA-4-VP2S-immunized mice had a 100-fold higher ELISA antibody and 10-fold higher HI antibody compared to VP2S-immunized mice.
经过30次循环PCR扩增后，琼脂糖凝胶电泳显示预期大小的扩增产物；序列测定结果显示，克隆的毕格犬CTLA-4胞外区与已发表序列的核苷酸同源性为99.2%，氨基酸序列同源性为98.4%，结合B7分子的六肽基序无变化； VP2S与已发表CPV VP2的核苷酸序列同源性为99%，氨基酸序列同源性为98.6%；经IPTG诱导后，两种重组大肠杆菌表达预期的29kDa VP2S和42kDa CTLA-4-VP2S重组蛋白，两者均能被CPV抗血清识别；间接ELISA和血凝抑制试验结果显示，CTLA-4-VP2S免疫组的抗体产生时间为初免后第2周，抗体高峰期为初免后第4周，而VP2S免疫组的抗体产生时间为初免后第4周，抗体高峰期为初免后第5周，两个试验组高峰期ELISA抗体效价和血凝抑制抗体效价分别相差100倍和10倍。
The variation coefficients were less than 15% in the intro-batch and in the inter-batch tests, indicating good interplate repeatability and intraplate reproducibility of the assay. There was 96.5% agreement in comparison with the commercial kit.
该方法与犬瘟热、犬传染性肝炎、犬副粘病毒病、犬波特氏杆菌病等4种犬常见传染病阳性血清无交叉反应，具有较好的特异性；批内、批间重复试验变异系数均小于15 %，显示该方法具有良好的重复性；与商品化CPV ELISA 试剂盒进行比较，符合率为 96.5 %。
Negative correlation were detected among IVOE and eight quality characters, namely, GL, LWR, AC, CPV, SBV, consistency viscosity, gelatinization temperature and protein content; while positive among the IVOE and GW, LT, TD, viscosity, elasticity and breakdown viscosity, respectively.
粒长、长宽比、直链淀粉含量、糊化温度、蛋白质含量、热浆粘度、冷胶粘度、回复值和消减值9个指标都与米饭综合口感呈极显著负相关，而粒宽、崩解值、米饭光泽度、柔软性、粘性和弹性6个指标都与米饭综合口感呈极显著正相关。
In this paper, the method integrating CPV350 inverter welding machine to arc welding robot control system is introduced.
介绍了CPV350型逆变电焊机与弧焊机器人控制系统的连接方法。
In the present work we have studied theses two complementary methods to identify photons in the electromagnetic spectrometer PHOS.
通常有两种方法来鉴别光子和其他粒子：之一，在PHOS前放置一带电料子反符合探测器CPV。
Species of protozoans was given their SPV in this dissertation.
文中提出了用CPV评价水污染程度的水质划分范畴。
The indirectELISA results showed that the mAbs revealed high titer from l:2×103 to 1:l.6×106. All of them reacted withRV SRV9 antigen and did not cross react with CDV, CPV, CAV and VSV, that demonstrated their goodspecificity by indirec ELISA and Western-blotting. In addition, C1, C4, C8, C9, C10, C13 and C15 mAbs canreact with RV SRV9, 8202, and CVS, which showed their similarity of reaction to RV.
间接ELISA 和Western-blotting 试验结果表明，15 株单抗均与RV SRV9 全病毒抗原反应，与不同科病毒犬瘟热病毒，犬细小病毒，犬腺病毒和水疱性口炎病毒均不发生交叉反应，显示这15 株单抗对RV 具有良好的特异性；其中C1、C4、C8、C9、C10、C13、C15 单抗可与SRV9、8202、CVS 3 个毒株反应，表明他们是针对狂犬病病毒共同抗原决定簇的单抗。
Translated Description: China Network is a provider of CPA exams, accounting, title examinations, CTA examination, registration CPV, senior accountant, accounting examinations, CTA performance queries, and content of the examination sites.
Description：中国财税网是一个提供注册会计师考试，会计职称考试，注册税务师考试，注册资产评估师，高级会计师，会计考试，注册税务师成绩查询等考试内容的网站。
It is nucleic acidhomology over 96% between CPV of Sichuan regions and the other reported referencevirus strain in abroad. It is identical of the mode of evolution with reported literature, but itdoes not come into being obvious a branch of CPV in china.
我国四川地区的CPV与所报道国外参考毒株的核酸的同源性超过96％，没有形成明显的中国CPV分支，进化方式与文献报道的进化方式一致。
The images in myocarditis-typeof CPV diasease showed several apparently different traits with those in enteritis-type ofCPV disease, namely, pericardiac effusion, geometrical changes of left ventricle, incrassation of ventricular septum moving lean to left, decreased moving extent ofventricular septum, dilation of right ventricle and ventricular wall hypertrophy.
结果显示，心肌炎型细小病毒病的超声心动图具有不同于犬肠炎型的明显特征，如心率不齐、心包渗出、左心室几何形态改变、室间隔增厚、右心室扩张、室间隔波动幅度降低、室间隔与左心室壁呈同向运动、右心室壁博幅增加等。
Until 1980 only one of these viruses was registered as a control agent, a CPV of the pine caterpillar Dendrolimus spectabilis.
直到1980年，只有其中的一种病毒被注册作为一种抑制剂，是一种植物松毛虫细胞质多面体病毒。
In Chapter three, the CPV information fusion, one of the main techniques of the CPV, is studied.
第三章研究了计算机泛视觉体系中的信息融合技术。
Now it is becoming one of the most important and difficult direction of CPV research.
然而，顾客感知价值来自顾客的主观感知，因此也受到顾客个体心理的影响。
CPV is all the benefits, which can be perceived by the customers.
客户感知价值是指客户所感知的解决方案能够为他带来的利益的总和。
Acquiring customer satisfaction and customer loyalty is always business`dream, and enhance CPV are thought to be one of the most effective method.
获得顾客满意和顾客忠诚一直是企业最关心的，而众多学者认为提高顾客感知价值是实现顾客满意和顾客忠诚最有效的途径。