Methods:All the 40 Winstar rats were divided into four groups:group A:the rats were fed with normal diet, group B:the rats were fed with only high|cholesterol diet(no vitamin D3), group C:the rats were treated with one intramuscularly injection of vitamin D3(300000 U/kg) and were denudated of endothelium, followed by high|cholesterol diet, group D:the rats were treated with 5% chitosan added into the diet besides treatment as group C. Ninety days later, the formation of atherosclerosis plaques and the activities of nitric oxide synthase in aortas in the four group rats were investigated.
40只Wistar大鼠均分为：A组；B组：饲以高脂饲料（不含维生素D3）；C组：一次性给予大鼠维生素D3（30万U/kg体重）肌肉注射，以球囊损伤主动脉内皮和饲以含维生素D3（1.25×106U/kg）的高脂饲料；D组在C组的基础上饲料中加入5%壳聚糖。90 d后检测主动脉动脉粥样硬化斑块形成及NOS的活性。
Each medicine used in the study totally has obivious effect on lens sodium-pump. Either on the molecular and cellular level or on the histological and organic level, they all have their distinctive demonstration. 1.Ouabain、Digoxine、DMSO decrease the expression of αsubunit of sodium pump on mRNA level, the cell morphology and ultramicroscopic structure demonstrated destructive effect, it indicates the cause of cataract of the medicines.2.D-thyroxine、Amphotericin B、Vitamin E increase the expression of αsubunit of sodium pump on mRNA level, from the demonstration of the cell morphology and ultramicroscopic structure, it indicates the medicines have protective or anti-distructive effect on lens.3.The medicines have isoform-specific action on lens sodium-pump, it indicates exploitting isoform-specific medicine has a promising clinical application.4. Lens sodium-pumps are inhibited probably lead to cataract, so in clinical medication, especially to the cardiovascular doctor, while he is using the sodium-pump inhibitors, he should pay more attention to adjust the dosage and duration of the drug therapy. 5. To creat the animal model of cataract through inhibiting lens sodium-pump, whether it can be a kind of classical method or not, it is worthy of further research.
本研究所选用的药物对晶状体钠泵均有明显的影响作用，在分子水平、细胞水平、组织器官水平均有特异的表现：1、哇巴因、地高辛、二甲基亚砜三种药物可降低晶状体钠泵α亚单位在 mRNA水平的表达，在细胞形态和超微结构上表现为损伤效应，提示了其潜在的导致白内障的可能。2、D甲状腺素、两性霉素B、维生素 E三种药物可升高晶状体钠泵α亚单位在 mRNA水平的表达，从细胞形态和超微结构上的表现，提示了其对晶状体有保护作用或抗损伤效应。3、药物作用于晶状体钠泵具有重整异构特异性，提示了开发出特异的具有重整异构作用的药物是极有临床应用前景的。4晶状体钠泵抑制后可能导致白内障的发生，故临床用药，尤其是心内科医生在使用这些钠泵抑制剂时应注意调整用药剂量及药物疗程。5 通过抑制晶状体钠泵来制作白内障动物模型能否成为一种经典的制作方法，还有待于进一步研究。
Three hundred and twenty-four crossbred (Landrace×Yorkshine) barrows with average body weight of 27 kg were randomly assigned to 6 dietary groups: group Ⅰ(chlorotetracycline group, added 50 mg/kg chlortetracycline in basal diet group Ⅱ(origanum oil group, added 20 mg/kg oreganum oil in basal diet group Ⅲ(probiotics group, added 1.5 g/kg probiotics in basal diet group Ⅳ(nutrient enhancement group, elevated dietary vitamin E, vitamin B family and micro elements Zn, Mn, I group Ⅴ(origanum oil+nutrient enhancement group group Ⅵ(probiotics+nutrient enhancement group).
试验选用平均体重27 kg，健康长×大杂交阉公猪324头，随机分为6组：Ⅰ组（金霉素组，在基础日粮中添加50 mg/kg金霉素）、Ⅱ组（牛至油组，在基础日粮中添加20 mg/kg牛至油）、Ⅲ组（益生素组，在基础日粮中添加1.5 g/kg益生素）、Ⅳ组（营养调控组，提高日粮中维生素E和B族维生素、微量元素Zn、Mn和I水平）、Ⅴ组、Ⅵ组。
Chicken Meal, Chicken, Brown Rice, Duck Meal, Barley, Oatmeal, Chicken Fat (Preserved With Natural Mixed Tocopherols), Potatoes, Carrots, Lamb Meal, Salmon Meal, Canola Oil, Natural Flavor, Dried Egg, Brewers Yeast, Whole Ground Flaxseed, Potassium Chloride, Choline Chloride, Taurine, Spinach, Parsley Flakes, Cranberry, Lysine, L-Carnitine, Yucca, Kelp, Zinc Proteinate, Vitamin E Supplement, Niacin, Manganese Proteinate, Copper Proteinate, Zinc Sulfate, Manganese Sulfate, Copper Sulfate, Thiamine Mononitrate (Vitamin B-1), Vitamin A Supplement, Biotin, Potassium Iodide, d-Calcium Pantothenate, Riboflavin (Vitamin B-2), Pyridoxine Hydrochloride (Vitamin B-6), Vitamin B-12 Supplement, Manganous Oxide, Sodium Selenite, Vitamin D Supplement, Folic Acid.
鸡肉粉；鸡肉，糙米，鸭肉，大麦，燕麦，鸡肉脂肪，马铃薯、胡萝卜，羊肉粉，鲑鱼粉，芥菜油，天然香料，蛋粉，啤酒酵母；卵磷脂；亚麻仔，氯化钾，氯化胆碱，菊糖；牛磺酸，菠菜，荷兰芹，蔓越莓，赖胺酸，左旋肉碱，丝兰，海带，锌蛋白，维生素E补充，菸碱酸，锰蛋白，铜蛋白，硫酸锌，硫酸锰，硫酸铜，硝酸硫胺明(维生素B1来源)，维他命A，维生素H，碘化钾，D-泛酸钙，维生素B-12 补充；核黄素(维生素B-2来源)，维生素D补充，叶酸。
Results:The intake of calorie was lower than consumption, even if patients intook more calorie than RDA, it suggests that caloric requirement of cancer patients who receive radiotherapy is higher than RDA. During the radiotherapy, the body weight decline in most patients because of high consumption and lower intake of calorie and protein, The intake of vegetables, Vitamin A, B and elements Ca and Cu are lower than requirement.
结果：(1)放疗肿瘤患者热能摄入量在高于正常人推荐量的状态下仍不能满足消耗，提示放疗肿瘤患者的热能需要量大于正常人推荐量；由于热能、蛋白质的高消耗，大多数肿瘤患者放疗过程中体重下降；蔬菜、元素钙、铜、维生素A、B摄入不足。
A method for determination of water soluble vitamin C, vitamin B 1, vitamin B 2 and vitamin B 5 in pollen by HPLC was reported. The four kinds of vitamins were sparated on a ODS (5 μm)column using a buffer solution of pH 5.01 phosphate salt methanol(75∶25, V/V)as the mobile phase at a velocity of 1 ml/min and detected by UV detector at 260 nm.
采用液相色谱法同步测定花粉中的水溶性维生素C、维生素B1(VB1)、维生素B2 (VB2)和维生素B5(VB5)，四种维生素在ODS 5 μm色谱柱上分离，用pH 5 。0 1的磷酸缓冲液甲醇(体积比为 75∶2 5)为流动相洗脱，流速为 1.0ml·min-1，波长为 2 6 0nm ，10min内实现良好分离。
METHODS:Ninety-two patients divided into 3 groups, A, B and C. Group A(F26, age 63 a±s 7 a) were treated with calcium gluconate(6 g·d-1, po, tid) for 6 mo. Group B(F38, age 64 a±7 a) were treated with vitamin D3 (300000 U, im, once a month) and calcium gluconate(6 g·d-1, po, tid) for 6 mo.
A组26例，年龄63 a±s 7 a，口服葡萄糖酸钙6 g·d-1，B组38例，年龄64 a±7 a，口服葡萄糖酸钙6 g·d-1加维生素D3 30万U，im，每月1次；C组28例，年龄68 a±9 a，口服阿仑膦酸钠片10 mg，qd加葡萄糖酸钙6 g·d-1，3组疗程均为6 mo。
With the future growth that can be added on to five tablespoons. 2, A, calcium: elemental calcium daily 300-500mg (1) Composite Calcium Granule: 2-3 packets a day (each contains calcium 100mg)(2) active calcium Chongji: Daily 4-6 packets (each containing Calcium 50mg) B, fill D: daily 400-800IU (1) concentrated cod liver oil drops: 2-4 drops per day (2) vitamin D2 candy: the daily 400-800IU (3) vitamin D3 injections: once a month 20-300000 IU attention to the above-mentioned to be a doctor`s prescription, prescribed application. C, to the sun to vitamin D, ultraviolet radiation to the skin 7 - dehydrocholesterol into vitamin D3, to help calcium absorption, could be a day outdoors on the sun for half an hour each afternoon.
以后随月增长可添加到五汤匙。2、A、补钙：每日元素钙300-500mg(1)复合钙冲剂：每日2-3包(每包含钙100mg)(2)活性钙冲剂：每日4-6包(每片含钙50mg)B、补D：每日400-800IU(1)浓缩鱼肝油滴剂：每日2-4滴(2)维生素D2糖丸：每日400-800IU(3)维生素D3针剂：每月一次，20-30万IU注意上述物均须医生处方，遵医嘱应用。C、向太阳要维生素D，紫外线要将皮下7-脱氢胆固醇转变成维生素D3，帮助钙的吸收，可每天户外上下午各晒半小时。
May be caused by rickets cranial side 2, should be given calcium and vitamin Baby D, and more sun: calcium: elemental calcium daily 200-300mg (1) Composite Calcium Granule: 2-3 packets a day (per contains calcium 100mg)(2) active calcium Chongji: Daily 4-6 packets (each containing calcium 50mg) B, fill D: daily 400-800IU (1) concentrated cod liver oil drops: 3-5 drops per day (2) Vitamin D2 candy: the daily 400-800IU (3) vitamin D3 injections: once a month, 20-300000 IUC, to the sun to vitamin D, ultraviolet radiation to the skin 7 - dehydrocholesterol into vitamin D3, calcium to help absorption, can be a day outdoors on the sun for half an hour each afternoon.
可能是佝偻病引起的方颅2、应给孩子补钙和维生素D，并多晒太阳：补钙：每日元素钙200-300mg(1)复合钙冲剂：每日2-3包(每包含钙100mg)(2)活性钙冲剂：每日4-6包(每包含钙50mg)B、补D：每日400-800IU(1)浓缩鱼肝油滴剂：每日3-5滴(2)维生素D2糖丸：每日400-800IU(3)维生素D3针剂：每月一次，20-30万IUC、向太阳要维生素D，紫外线要将皮下7-脱氢胆固醇转变成维生素D3，帮助钙的吸收，可每天户外上下午各晒半小时。3
Methods85AsC were randomly divided into two groups, treatment group(n=45) and control group(n=40).The treatment group were treated with hepatitis B vaccine (20μg subcutaneously every 2 weeks) combined with anti-HBV-iRNA(4mg intramusculaly every 2 days)for six months, the control group were given glucurone and vitamin C.
方法85例AsC随机分为观察组45例和对照组40例，观察组采用乙肝疫苗(20μg，上臂三角肌皮下注射，每2周1次)联合抗乙肝免疫核糖核酸(4mg，肌肉注射，隔日1次)治疗，疗程6个月，对照组采用肝泰乐、维生素C口服治疗。
ObjectiveTo investigate therapeutic effectiveness of hepatitis B vaccine combined with anti-HBV-iRNA on chronic asymptomatic carriers with HBV. Methods85AsC were randomly divided into two groups, treatment group(n=45) and control group(n=40).The treatment group were treated with hepatitis B vaccine (20μg subcutaneously every 2 weeks) combined with anti-HBV-iRNA(4mg intramusculaly every 2 days)for six months, the control group were given glucurone and vitamin C.
目的研究乙肝疫苗联合抗乙肝免疫核糖核酸对慢性无症状乙肝病毒携带者(chronic asymptomatic carries，AsC)的抗病毒治疗作用方法85例AsC随机分观察组45例和对照组40例，观察组采用乙肝疫苗(20μg，上臂三角肌皮下注射，每2周1次)联合抗乙肝免疫核糖核酸(4mg，肌肉注射，隔日1次)治疗，疗程6 月，对照组采用肝泰乐、维生素C口服治疗。
Calcium deficiency can cause occipital bald and rib eversion should be given calcium and vitamin D your baby and more than the sun: A, calcium: elemental calcium daily 300-500mg (1) Composite Calcium Chongji: 3 packets per day (each containing Calcium 100mg)(2) Renal bone胶襄: day one (Each calcium 250mg)(3) Gaitianli: 6 per day (calcium per tablet 50mg) B, fill D: daily 400-800IU (1) concentrated cod liver oil drops: 3-5 drops per day (2) vitamin D2 candy: the daily 400-800IU (3) vitamin D3 injections: once a month, 20-300000 IUC, to the sun to vitamin D, ultraviolet to subcutaneous 7 - dehydrocholesterol into vitamin D3, to help calcium absorption, could be a day outdoors on the sun for half an hour each afternoon.
缺钙可引起枕秃和肋骨外翻，应给孩子补钙和维生素D并多晒太阳：A、补钙：每日元素钙300-500mg(1)复合钙冲剂：每日3包(每包含钙100mg)(2)肾骨胶襄：每日1粒(每粒含钙250mg)(3)盖天力：每日6片(每片含钙50mg)B、补D：每日400-800IU(1)浓缩鱼肝油滴剂：每日3-5滴(2)维生素D2糖丸：每日400-800IU(3)维生素D3针剂：每月一次，20-30万IUC、向太阳要维生素D，紫外线要将皮下7-脱氢胆固醇转变成维生素D3，帮助钙的吸收，可每天户外上下午各晒半小时。
Calcium, blood calcium decreased, the brain caused by increased nerve excitability plant, resulting in baby night waking, night terrors, night irritability, sleep; baby should be given calcium and vitamin D and more sun: A, calcium: elemental calcium daily 300-500mg (1) Composite Calcium Granule: 3-5 packets a day (each contains calcium 100mg)(2) Renal bone胶襄: 1-2 tablets daily (each piece costs calcium 250mg)(3) cover Tianli: 6-10 tablets per day (calcium per tablet 50mg) B, fill D: daily 400-800IU (1) concentrated cod liver oil drops: 5-8 drops per day (2) vitamin D2 candy: Daily 400-800IU (3) vitamin D3 injections: once a month, 20-300000 IUC, to the sun to vitamin D, ultraviolet radiation to the skin 7 - dehydrocholesterol into vitamin D3, to help calcium absorption, could be a day of outdoor the afternoon sun for half an hour.
缺钙、血钙降低，引起大脑植物性神经兴奋性增高，导致孩子夜醒、夜惊、夜间烦躁不安，睡不安稳；应给孩子补钙和维生素D并多晒太阳：A、补钙：每日元素钙300-500mg(1)复合钙冲剂：每日3-5包(每包含钙100mg)(2)肾骨胶襄：每日1-2粒(每粒含钙250mg)(3)盖天力：每日6-10片(每片含钙50mg)B、补D：每日400-800IU(1)浓缩鱼肝油滴剂：每日5-8滴(2)维生素D2糖丸：每日400-800IU(3)维生素D3针剂：每月一次，20-30万IUC、向太阳要维生素D，紫外线要将皮下7-脱氢胆固醇转变成维生素D3，帮助钙的吸收，可每天户外上下午各晒半小时。
2 Is a calcium deficiency, it should be calcium and vitamin D and more sun: A, calcium: elemental calcium daily 300-500mg (1) Composite Calcium Granule: 3-5 packets a day (each contains calcium 100mg)(2) Renal bone胶襄: 1-2 tablets daily (each piece costs calcium 250mg)(3) Gaitianli: 6-10 tablets per day (calcium per tablet 50mg) B, fill D: daily 400-800IU (1) concentrated drops cod liver oil: 5-10 drops per day (2) vitamin D2 candy: the daily 400-800IU (3) vitamin D3 injections: once a month, 20-300000 IUC, to the sun to vitamin D, ultraviolet to subcutaneous 7 - dehydrocholesterol into vitamin D3, to help calcium absorption, could be a day outdoors on the sun for half an hour each afternoon.
是缺钙2、应该补钙和维生素D并多晒太阳：A、补钙：每日元素钙300-500mg(1)复合钙冲剂：每日3-5包(每包含钙100mg)(2)肾骨胶襄：每日1-2粒(每粒含钙250mg)(3)盖天力：每日6-10片(每片含钙50mg)B、补D：每日400-800IU(1)浓缩鱼肝油滴剂：每日5-10滴(2)维生素D2糖丸：每日400-800IU(3)维生素D3针剂：每月一次，20-30万IUC、向太阳要维生素D，紫外线要将皮下7-脱氢胆固醇转变成维生素D3，帮助钙的吸收，可每天户外上下午各晒半小时。
Contains more vitamin B, vitamin B, vitamin C 12 and coarse fibers.
含有较多的维生素B、维生素B、维生素C 1 2和粗纤维。
This product contains: 150 mg Thiamin (Vitamin B-1), 150 mg Riboflavin (Vitamin B-2), 150 mg Niacin, 100 mg Vitamin B-6, 400 mcg Folic Acid, 150 mcg Vitamin B-12, 150 mcg Biotin, 150 mg Pantothenic Acid, 150 mg Choline Bitartrate, 150 mg PABA, and 150 mg Inositol. See Supplement Facts for additional information.
该产品包含：150毫克硫胺素（维生素B - 1 ），150毫克核黄素（维生素B - 2 ），150毫克尼克酸，100毫克维生素B - 6 ，400微克叶酸，150毫克维生素B - 12 ，150微克生物素，150毫克泛酸，150毫克胆碱酒石酸，苯甲酸150毫克，150毫克和肌醇。
Patients received single tablet of B vitamins containing folic acid (2.5 mg/d), vitamin B6 (25 mg/d), and vitamin B12 (1 mg/d), or matching placebo.
患者随机的服用单种B族维生素胶囊，包括2.5mg/日的叶酸，25mg/日的维生素B6.1mg/日的维生素B12，以及安慰剂。
Higher rates of anemia and ricket, however, were observed, which reached 22.5% and, 52.0% respectively. Average intake of protein, energy, vitamin A, B1, B2 were approached or reached the National RDA.
幼儿膳食摄入的蛋白质、热能、维生素B_1、B_2、PP及A的平均水平接近或达到了标准供给量，铁的供给量虽高，但优质来源少；钙、锌的摄入则严重不足。
Determination of the concentration of Vitamin B_1 by the method of automatic potentiometric titration was studied by using silver electrode as indicator electrode and saturated calomel electrode as reference electrode.
研究了以银电极为指示电极，饱和甘汞电极为参比电极，用自动电位滴定法测定维生素B1含量。
RESULT: 1.Ouabain act on lens sodium-pump, under the LM, lens anterior capsular membrane discontinuous, epithelium cells clustered, occluding zonule seperated, lens fiber layers fractured. Under the EM, cells totally hollowed, mitochondria swelling, myelin figure appeared. RT-PCR examine the expression condition of αsubunit of sodium pump on mRNA level, α1、α2 and α3-isoform are all decreased.2.Digoxin act on lens sodium-pump, under the LM, lens cell oedema, linkage distructed, extensive exfoliation. Under the EM, plasma appeared little half-transparant hollow region, mitochondria swelling and ridge disappeared. RT-PCR examine, α1、α2 and α3-isoform are all decreased.3.Amphotericin B act on lens sodium-pump, under the LM, lens epithelium cells linked tightly, arranged in-line, lens fiber layers arranged tightly and regularily. Under the EM, abbundant cellular organes, exuberant cells function indicated. RT-PCR examine the expression condition of αsubunit of sodium pump on mRNA level, α1 and α3-isoform are increased significantly, demonstrated isoform-specific action.4D-thyroxine act on lens sodium-pump, under the LM, lens plasmalemma integrated, cells arranged tightly and regularily. Under the EM, nucleus fission appeared, desmosome half-desmosome and tensile microfilaments linked the cells. RT-PCR examine, α2 and α3-isoform are increased, also demonstrated isoform-specific action.5.Vitamin E act on lens sodium-pump, under the LM, lens anterior capsular membrane continuous and smooth, epithelium cells tightly linked, lens fiber layers appearede hollow region occasionally. Under the EM, lateral membrane high density belt appeared, abundant nucleolus. RT-PCR examine, onlyα1-isoform are increased, demonstrated significantly isoform-specific action.6.DMSO act on lens sodium-pump, under the LM, lens anterior capsular membrane slightly thicker, cells linkage partly distructed. Under the EM, plasmalemma denaturation, mitochondria swelling. RT-PCR examine, α1、α2 and α3-isoform are all altered slightly and haven`t significant meanning.
结果：1、哇巴因作用于晶状体钠泵后，光镜下晶状体前囊膜断裂、上皮细胞聚积、闭合连接分离、纤维板层裂隙，电镜下全层细胞空泡化、线粒体肿胀出现髓样结构，RT-PCR法检测晶状体钠泵α亚单位α1、α2及α3三种重整异构体在mRNA水平的表达均减弱。2、地高辛作用于晶状体钠泵后，光镜下晶状体细胞水肿、细胞连接破坏、广泛剥离，电镜下胞质见少许半透明空化区、线粒体肿胀嵴消失，RT-PCR法检测晶状体钠泵α亚单位α1、α2及α3三种重整异构体在mRNA水平的表达均减弱。3、两性霉素B作用于晶状体钠泵后，光镜下晶状体上皮细胞紧密连接、线状排列、纤维板层紧密规整，电镜下细胞器丰富、细胞功能旺盛，RT-PCR法检测α1及α3表达显著增强、具有一定的重整异构作用特异性。4、D甲状腺素作用于晶状体钠泵后，光镜下晶状体质膜完整、细胞排列紧密规整，电镜下胞核见分裂像、细胞间有桥粒、半桥粒及张力微丝，RT-PCR法检测α2及α3表达均增强、亦有一定的重整异构作用特异性。5、维生素E作用于晶状体钠泵后，光镜下晶状体前囊膜连续光滑、上皮细胞紧密连接、纤维板层偶见空化，电镜下囊侧膜内有高电子密度带、细胞核仁丰富，RT-PCR法检测仅有α1的表达显著增强、具有极强的重整异构作用特异性。6、二甲基亚砜作用于晶状体钠泵后，光镜下晶状体前囊膜轻度增厚、细胞连接部分破坏，电镜下质膜变性、线粒体肿胀，RT-PCR法检测晶状体钠泵α亚单位α1、α2及α3三种重整异构体在mRNA水平的表达无显著改变。