All of the 58 restriction endonucleases were used to investigate the mtDNA diversity. Four restriction endonucleases, DraⅠ、SnaBⅠ、SspⅠ and Vsp Ⅰ, were polymorphic. Based on presence or absence of the the 215〓 SspⅠ site, all of the 13 mtDNA sequences were divided into two groups.
利用DNAClub软件对AT富集区和tRNA〓基因的DNA序列进行了模拟酶切，所有的58种内切酶中有10种内切酶在13个材料中有酶切位点，仅4种酶DraⅠ、SnaBⅠ、SspⅠ和VspⅠ表现出多态性，且主要位点差异在于SspⅠ酶切位点的有无（序列的第215位的C/T）。12个品种中有4个品种（豫早1号、豫早2号、鲁红、33）没有此酶切位点，另外8个品种有此酶切位点，野柞蚕也没有此酶切位点。
Omparison of the amino acid homology with COX3 genes of other eleven arthropods was made.
OX3的下游为 6 6bp的tRNA Gly基因。蓖麻蚕的COX3与家蚕COX3同源性最高，核苷酸和氨基酸序列同源性分别是 80 2 %和 85 6 %。
Analyzing by blastn program of NCBI, the fragment includes: 30bp 12srRNA partial sequence, 71bp tRNA-Val complete sequence and 913bp 16srRNA partial sequence, and the homolog is more than 95% compared with other Camelidae animals.
PCR条件优化后成功扩增出长度为1014 bp的DNA片段，blastn分析显示，该片断与骆驼科动物的同源性高于95%，所获得片断包括30 bp的12srRNA基因部分序列，71bp的tRNA-Val基因全序列和913bp的16srRNA基因部分序列。
In the process of protein synthesis, a transfer RNA pairs with the mRNA codon.
在蛋白质接合的过程中，一个tRNA和mRNA密码子结合，那tRNA的反密码子核甘酸序列是？
These works will lay a solid foundation for further research on the mechanism of half-tRNAs'biogenesis.
本研究首次在哺乳动物细胞中发现了half-tRNA，并揭示了half-tRNA产生的原因及其形成机制，这些工作为全面研究half-tRNA生成的机制和生物学功能奠定了良好的基础。
The middle and small breed dogs were clustered at first; German Sheepdog, Swedish Elkhound, and Black Russian Terrier were clustered into one group, and the Tibetan Mastiff, Old English Sheepdog, Leonberger, and Saint Bernard were clustered in another group.
本文根据家犬线粒体基因组DNA序列设计引物，获得了2，525bp的藏獒线粒体DNA序列（包括细胞色素b、tRNA-Thr、tRNA-Pro和控制区全序列），并以灰狼、郊狼作为外类群对藏獒、12个家犬品种进行了系统发育分析。
Objective To assess the prevalence of mitochondrial Trna leugene mt 3243 A→G mutation and ND-1 gene mt 3316 G→A mutation in Chinese type 2 diabetes.
目的为了解线粒体tRNALeunt3243A-G突变和ND-1基因nt3316G→A突变在中国人2型糖尿病人群中的精确发病率及该基因突变所导致的Ⅱ型糖尿病的临床特征。
Escherichia coli 4-fluorotryptophan-substituted arginyl-tRNA synthetase was biosynthetically prepared and purified from a tryptophan auxotroph, which could overproduce this enzyme.
利用色氨酸营养缺陷型菌株在大肠杆菌精氨酰tRNA合成酶中成功地掺入了4-氟-色氨酸。
Finally, the mutated argS genes, in which the codons for tryptophan were singly changed to those for alanine, were recombined to the plasmid pTrc99B and transformed to an Escherichia coli tryptophan auxotroph. 4-F-Trp was then biosynthetically incorporated into the mutant enzyme ArgRS162WA, allowing that 4-F-Trp-labeled ArgRS162WA was purified to large scale.〓F NMR of 4-F-Trp-labeled ArgRS162WA revealed that Trp〓 was near or in the arginine-binding site of arginyl-tRNA synthetase.
用高度纯化的tRNA〓和tRNA〓进行〓F核磁共振研究，发现两种tRNA〓等受体和精氨酰tRNA合成酶结合后的〓F核磁共振谱明显不同，揭示二者不同的结合情况，为用〓F核磁共振研究氨酰tRNA合成酶和tRNA等受体的结合提出了一种新思路。
Pheny lalanyl tRNA synthetase, eukaryotictranslation elongation 〓 genes may be related directly to changes ofphenotypes of induicible cells.
这些基因的变化不仅为基因治疗靶点的选择提供了依据，而且为寻找导致肿瘤发生主要因素提供参照。
It is suggested that most of them have classical cloverleaf structures, each arm with high base pair ratio and good stability.
结果表明：8个tRNA基因具有较为典型的三叶草型结构，各臂的碱基配对率大多数较高，稳定性较好。
The whole chloroplast genome includs four ribosomal RNA, thirty tRNA genes transfer 20 species of amino acids, 74 protein-encoding genes and 2 for unidentified open reading frame.
整个叶绿体基因组包括4种核糖体RNA，30个基因编码20种转运RNA，74个蛋白质编码基因和2个未知功能的开放阅读框。
Some of these enzymes hae two main functional parts, or actie sites: a site that links the amino acid to the tRNA, and a separate editing site that proofreads this process and remoes wrongly added amino acids.
这些酶中有些酶具有两个主要功能部分或活性位点；一个位点用于连接氨基酸和tRNA，另一个独立编译位点用于校正翻译过程并移除错误的氨基酸。
In order to gain evidece of nontargeted mutagenesis in mammalian cells, the simian virus 40based shuttle plasmid pZ189 was used as a molecular probe. This plasmid can replicate both in monkey cells and bacterial E. coli cells and contains the bacterial supF suppressor tRNA gene as an easily sequenced target.
为获得哺乳类细胞中非定标性突变的证据，实验选用以SV40为基础的穿梭质粒作为探针。pZ189能在猴细胞和大肠杆菌中复制，其靶基因supF tRNA基因很容易进行测序。
The stem regions were relatively conserved, and the variable base pairs were under the restriction of compensatory changes or G-U wobble pairing which could be regarded as mechanisms for maintaining a stable secondary structure.
通过比较这些tRNA基因序列和二级结构差异，发现核苷酸变异主要出现在环区，且存在插入缺失。
Three major clusters of mitochondrial tRNA genes tRNAIle-tRNAGln-tRNAMet, tRNATrp-tRNAAal-tRNAAsn-tRNACys-tRNATyr and tRNAHis-tRNASer(AGY-tRNALeu from 13 species of Predatory birds were amplified and sequenced. The length of these tRNA clusters was similar among species (212~214 bp, 353~362 bp, 205~208 bp, respectively), and 47% of the sequences were variable, 67% of which were involved in the loop regions. The stem regions were relatively conserved, and the variable base pairs were under the restriction of compensatory changes or G-U wobble pairing which could be regarded as mechanisms for maintaining a stable secondary structure.
利用PCR方法扩增猛禽类13种鸟类线粒体基因组中3个主要的tRNA基因簇：IQM (tRNAIle-tRNAGln-tRNAMet)、WANCY(tRNATrp-tRNAAla-tRNAAsn-tRNACys-tRNATyr)和HSL（tRNAHis-tRNASer-tRNAleu），测序后结合GenBank已登陆的游隼和普通鵟相应序列探讨猛禽类共15种鸟类的分子系统进化。3个目的片段长度分别为212～214 bp、353～362 bp、205～208 bp，通过比较这些tRNA基因序列和二级结构差异，发现可变核苷酸位点占47%，这些变异中67%出现在环区，且存在插入缺失。
The results indicate that the flexibility of the first base pair can affect the editing reaction catalyzed by LeuRS.
这些结果表明tRNA〓接受茎上第一对碱基配对的柔性程度能够影响LeuRS的编校。
A single point mutation in intervening sequence 2 (IVS2) of human mitochondrial arginyl-tRNA synthetase gene causes abnormal cleavage of its transcript, resulting in pontocerebellar hypoplasia.
人线粒体精氨酰-tRNA合成酶基因2号内含子中的一个单点突变导致该基因的转录本被异常剪接，造成脑桥小脑发育不全。
We report that the mouse sticky mutation, which causes cerebellar Purkinje cell loss and ataxia, is a missense mutation in the editing domain of the alanyl-tRNA synthetase gene that compromises the proofreading activity of this enzyme during aminoacylation of tRNAs.
我们的研究发现老鼠中一个可以导致小脑Purkinje细胞功能丧失和运动失调的关键性突变，这一关键性的突变发生在alanyl转运RNA合酶基因的编缉编码区，致使酶促转运RNA 氨基乙酰化的活性丧失。
The average A+T content of the A. melete and E. pyretorum mitochondrial genome protein coding sequence, rRNA and tRNA gene was corresponding well to the A+T bias generally observed in insect mitochondrial genomes.
黑纹粉蝶A.melete和樟蚕E.pyretorum线粒体基因组的蛋白编码基因、tRNA基因、rRNA基因和AT富含区均存在很强的碱基A+T含量偏向性。