Objective To investigate whether the tyrosine protein kinase Src and Fyn were involved in the neuronal cell death induced by cerebral ischemia followed by reperfusion.
目的 观察胞内可溶性酪氨酸蛋白激酶Src、Fyn蛋白对脑缺血再灌注后海马CA1区神经细胞存活的影响。
Both Src and Fyn play an important role in neuronal death induced by cerebral ischemia followed by reperfusion, while Src dominate over Fyn in the process.
胞内可溶性酪氨酸蛋白激酶Src、Fyn蛋白的含量降低对脑缺血再灌注后海马神经细胞有明显保护作用，且以Src作用更为明显。
Since Src could affect tumorigenesis and cell movement, it was likely that Tyr-45 phosphorylation (Pi-Tyr-45) played a critical role in p97Eps8-mediated tumorigenesis and migration.
由於 Src 可以影响细胞之生长与移动，故吾人不禁要问 p97Eps8 Tyr-45 的磷酸化是否与 p97Eps8 之参与细胞生长与移动有关？
The human c-src gene was obtained by PCR techniques from the mRNA of human pulmonary cancer cells and plasmid pcDNA3.1/Hygro contained the c-src sequence.
本文通过构建特异性c-src的原核表达重组质粒，转化大肠杆菌克隆表达得到完整的人源c-Src蛋白。
We presume that Hsp90 may also possibly chaperone PKC-ε translocation, as it does for v-Src activation on the plasma membrane.
我们认为有可能与以下两种因素有关。进行了Hsp90β和PKCε两者间的免疫共沉淀实验。
Besides, the tyrosine phosphorylation of Src, caveolin and Met was also increasd at this stage.
此外，在此时期Src、caveolin及Met的酪胺酸磷酸化也会增加。
Using a novel and specific Src kinase oral inhibitor AZD0530, they demonstrated inhibition of growth and metastases in vivo.
使用新的特效的口服的Src激酶抑制剂AZD0530，他们显示了在体内对增殖和转移的抑制。
Furthermore, we found that LPS could upregulate the expression of c-Src protein that in turn caused the enhancement of tyrosyl phosphorylation of Shc and ERK and promoted cellular growth.
此外，另一个Src的受质，FAK，其tyrosyl phosphorylation也因LPS促进Src整体活性提高而增加，而这可能与细胞的移动有关。
Interestingly, accompanying with downregulation of c-Src in curcumin-treated cells was the reduced tyrosyl phosphorylation and the enzymatic activity of FAK, an important player in integrin signaling.
另外让我们感到有趣的是，FAK，一个在integrin signaling pathway上重要的调节蛋白，它的autophosphorylation state竟也会随著curcumin浓度的增加而下降。
Therefore, we plan to utilize murine p68Eps8 cDNA as a probe to screen its human homolog and study its effects in tumorigenesis and cell migration. Besides, the functional implication of tyrosyl phosphorylated p68Eps8 will also be addressed.
由於 p68Eps8 在 v-Src 癌化细胞里亦呈高度磷酸化，故找出 Src 磷酸化 p68Eps8 的位置，并探讨其磷酸化后的生理意义，亦将是本计划的重要研究课题。
In this paper, normal section bearing capcity of SRC beam is analyzed from three aspects on the basis of force character of upper flange of shaped steel in SRC beam.
文中根据型钢混凝土梁中型钢上翼缘的受力特点分三种情况对型钢混凝土梁的正截面承载力进行了分析。
Based on the study of the handbook, its calculation assumption is improved, equivalent stress method is used to calculate bending strength of SRC beams with I shaped steel member and new calculation formulas are derived.
关于矩形截面工字型钢混凝土梁抗弯承载力的计算，国内目前尚无有关的规范，针对这一情况开展了相关研究；基于《钢与混凝土组合结构设计施工手册》的研究成果，对《手册》方法的计算假定作了若干改进，采用了等效应力法计算此类构件的抗弯承载力，得到了相关计算式；计算式适用于截面非对称配筋的情况。
Furthermore, the activation domain 1 (AD1) of SRC-1 was required for the coactivation of hB1F, and that was synergied by the general coactivator CREB-binding protein.
SRC-1对hB1F的共激活作用需要其激活结构域1(activation domain 1，AD1)的参与，并与通用辅激活子CBP（CREB-binding protein）协同，提示组蛋白乙酰化在受体转录激活中的重要作用。
The results show that prices of real estates are associated with inflation rate, location (the 7th urban planning area), types of houses (e. g., townhouse, apartments, and mansion), use of housing (commercial, office, and industrial uses), and building construction (i. e., RC, SC, and SRC).
实证后发现，通货膨胀率对房价有显著影响；亦有证据显示七期重划区之房价高於其他地区；在房屋的类型及用途中，以透天、店铺及商业用途的房屋价格较高，房屋结构(钢筋混凝土、钢骨混凝土)亦对房价有显著影响。
Objective To investigate whether tyrosine protein kinases Src and Fyn were involved in the phosphorylation of NR2A induced by cerebral ischemia followed by reperfusion. Methods Transient (15 min) brain ischemia was induced by the four-vessel occlusion in Sprague-Dawley rats. The antisense oligodeoxynucleotides of Src and Fyn were used to suppress the expression of Src and Fyn by intracerebroventricular infusion once a day for 3 days before ischemia.
近年来，最受人们关注的缺血性脑损伤机制是兴奋性氨基酸（excitatory amino acid，EAA）的神经毒性作用及神经元内钙超载触发的级联反应［1］，其中NMDA受体的NR2亚基在缺血性脑损伤中起着非常重要的作用，在缺血/再灌注（ischemia/ reperfusion，I/R）过程中其蛋白表达、磷酸化水平均有改变。
Results Both Src and Fyn antisense ODNs showed a protective role against neuronal cell death induced by cerebral ischemia/reperfusion. Furthermore, administration of Src antisense ODNs had more protective effect on the neuronal death than Fyn antisense ODNs.
结果 Src、Fyn反义寡核苷酸对脑缺血再灌注后海马CA1神经细胞均有明显保护作用，以Src AS ODNs保护作用更明显，与缺血组比较约有50%细胞存活(P.05)。
Western blot was used to examine the phosphorylation of NR2A in rat hippocampus. Results Both Src and Fyn antisense ODNs could decrease the phosphorylation of NR2A induced by cerebral ischemia/reperfusion, while administration of Src antisense ODNs had more suppressive effect on the phosphorylation of NR2A than Fyn antisense ODNs.
对NR2亚基有磷酸化调节作用的是Src家族酪氨酸蛋白激酶（Src family of protein tyrosine kinase，Src PTKs），这是一类膜结合的非受体酪氨酸蛋白激酶；现已知Src家族成员中Src、Fyn、Yes、Lyn、Lck在中枢神经系统中均有表达。
Methods Transient (15min) brain ischemia was induced by the four-vessel occlusion in Sprague Dawleyrats. The antisense oligodeoxynucleotides of Src and Fyn were used to suppress the expression of Src and Fyn by intracerebroventricular infusion once per day for 3 days before ischemia. After 5 days reperfusion, rats were perfusion-fixed with paraformaldehyde and cresyl violet staining were used to examine the survival of CA1 pyramidal cells of hippocampus.
采用Pulsinelli-Brierley 4动脉阻塞(4-VO)大鼠全脑缺血模型，缺血前连续3d脑室注射Src、Fyn反义寡核苷酸(antisense oligodeoxy-nucletides，AS ODNs)抑制Src、Fyn蛋白的表达后缺血，复灌5d，石蜡切片，焦油紫染色，图像分析测定单位面积内焦油紫染色细胞面积总和，与缺血组及错义寡核苷酸组(missense oligodeoxy-nucletides，MS ODNs)比较进行形态学分析。
This step, moving a single plate from stand Src to stand Dest, is trivial.
这一步，移动，从一个单一的立场钢骨混凝土板站DeST的，实在是微不足道。
This paper discuss the key techniques used in Stroke to Raster Scan Conversion: The fundamentals of video scan conversion. A practical scan conversion system based on CPLD is proposed, in which CPLD is used to control SRC system flexibly and it realizes stroke to taster scan display system conversion.
本论文分析了随机扫描到光栅扫描转换的关键技术，即视频扫描转换的基本原理，并给出了一种实际实现方案，构建了以CPLD为控制核心的扫描转换硬件系统。