AIM: To determine the cleavage activity of anti-transforming growth factor β1 hammerhead ribozymes which was inserted into U1 small nuclear RNA in cell-free system.
目的：研究抗转化生长因子β1 U1snRNA嵌合型锤头状核酶的细胞外切割活性。
Conclusion We have successfully constructed the recombinant PUC-hU_6-extra plasmid vector that can efficiently transcribe small RNA molecules directed by hU_6 snRNA promoter in the gastric carcinoma cells & SGC-7901.
结论成功构建了以hU6snRNA启动子驱动的、能在人胃低分化腺癌SGC-7901细胞内高效转录小分子RNA的表达质粒PUC-hU6-extra。
Role of SV40 T antigen binding sites within the 5'flanking region of human u_1 and u_2 snRNA genes
人U1和U2snRNA基因5'端区域中SV（40）T抗原结合位点的作用
The snRNA species extracted were the same as that prepared from purified antigens.
其结果与纯化抗原的小核RNA组成相同。
Using DNA immunoassays we have shown that the 5'- flanking region of the genes encoding human Ul and U2 snRNA contain regions that are able to bind tightly and specifically with the SV40 large tumor antigen (T Ag).
利用DNA结合免疫分析证明，编码人U1和U2snRNA基因的5'端区域含有与SV40T抗原特异结合的序列。
SnRNA and Antisense RNA
核内小RNA与反义RNA
RNA (tRNA, rRNA, mRNA, and SnRNA) has two main functions: First, it is genetic materials of some viruses.
RNA（即tRNA，rRNA，mRNA和SnRNA）有两大主要功能：一是某些病毒的遗传物质；