Preparation of bacillus pumilus suspension: Inoculate the nutrient agar slant with bacillus pumilus to the culture bottle that contains nutrient agar. Keep it in 35-37℃ for 7 days, and then use Gram-stain method to do smear microscopy. The rate of gemma should be above 85%.
短小芽孢杆菌悬液取短小芽孢杆菌的营养琼脂斜面培养物，接种于盛有营养琼脂培养基的培养瓶中，在35-37℃下培养7日，用革兰染色法涂片镜检，应有芽孢85%以上。
A satisfied cytologic smear, improvement of the smear preparation, understanding the particularity of the disease and cytologists`s skill in diagnosis are critical to decrease the false-negative rate.
获取满意的宫颈细胞样本、改进标本处理、认识到病灶的特殊性以及加强细胞学医师的学习和提高自身诊断水平是降低TCT假阴性的有效方法，临床医师对症状体征可疑但细胞学阴性的病例采取阴道镜下多点活检可减少假阴性的发生。
A satisfied cytologic smear, improvement of the smear preparation, understanding the particularity of the disease and cytologists's skill in diagnosis are critical to decrease the false-negative rate. It's important that gynecologis do multiple biopsy under vaginoscope to the patients who are suspicious of cancer but negative cytology.
获取满意的宫颈细胞样本、改进标本处理、认识到病灶的特殊性以及加强细胞学医师的学习和提高自身诊断水平是降低TCT假阴性的有效方法，临床医师对症状体征可疑但细胞学阴性的病例采取阴道镜下多点活检可减少假阴性的发生。
The test of tct has significant difference between smear with blood cells and smear without blood cells(χ2=34.040, p=0.000).conclusion a satisfied cytologic smear, improvement of the smear preparation, understanding the particularity of the disease and cytologists`s skill in diagnosis are critical to decrease the false-negative rate.
2=34.040，p=0.000）。结论获取满意的宫颈细胞样本、改进标本处理、认识到病灶的特殊性以及加强细胞学医师的学习和提高自身诊断水平是降低tct假阴性的有效方法，临床医师对症状体征可疑但细胞学阴性的病例采取阴道镜下多点活检可减少假阴性的发生。
Results The amaranthine nuclei had a distinct outline and distributed evenly on the slide. The area of nuclei in liquidbased preparation was much smaller and chromatin was more condensed in comparison with those in conventional smear. The nuclei in conventional smears had the largest value of area and the value of AOD, and CV of IOD, the IOD ratio among diploid, tetraploid and octaploid DNA was most close to 2 and 4, and the CV of IOD exceeded 6%.
结果 三种涂片内肝细胞核分布均匀，轮廓清晰，呈紫红色，与传统涂片法相比，液基法内肝细胞核面积明显减小，染色质高度浓缩；相同倍体的肝细胞核在传统涂片中面积最大，平均光密度最低，各倍体间的比值最接近2和4，积分光密度CV值最低（。5）。
It also may come about because: when the smear was taken, the brush or spatula did not scrape over the area containing the cancer, the cells may have gotten washed off the slide during the preparation and staining of the slide, the slide may have gotten mixed up with somebody else's slide, or the cytotechnologist reading the slide didn't see them.
它也可能是因为：当涂片采取了，刷或spatula没有刮该地区包含癌症，细胞可能得到的水洗过幻灯片期间的制备及染色，幻灯片，投影片，可能得到的混合与别人的幻灯片，或细胞化学检验家读幻灯片时没有看到他们。
Methods Based on the illness state and infection site, pathogenic microbes were examined by culture of blood, sputum, secretion, pleural fluid, peritoneal fluid and urine and smear preparation of sputum and stool, respectively.
方法针对不同的晚期肿瘤患者，根据病情及感染部位分别进行血培养、痰培养、分泌物培养、胸水或腹水培养、尿培养、痰涂片、以及大便涂片，检测并鉴别致病菌。