After 8-10 hours digestion at 4 ℃, the epidermal cells could be separated from dermis completely. The epidermal cells were crashed with the keratinocyte serum free medium﹙K-SFM﹚repeatedly to harvest epidermal single cell suspension, then the cells were vaccinated in the pretreatment collagen Ⅳ culture plate and placed in an incubator of saturated humidity at 37 ℃, with 0.05 volume faction of CO2 nutritive medium. With 20 minutes cultivation, the cultural fluid and the non-attaching cell were got rid of and added to nutritive medium which contained 0.1 volume fraction of fetal bovine serum, 10 μg/L epidermal growth factor and keratinocyte serum free medium. When 70%-80% cells were mixed, the cells were subcultured. The non-attaching keratinocyte cells were vaccinated in other culture plate as the control groups.
无菌条件下取胎儿皮肤标本，剪成3.0 mm×5.0 mm皮条片，4 ℃胰蛋白酶+EDTA联合消化8~10 h，表皮真皮分离，用角质细胞无血清培养基将表皮反复吹打，获得单细胞悬液接种于预处理好的Ⅳ型胶原培养板，置37 ℃、体积分数为0.05的CO2饱和湿度培养箱中，20 min后吸去培养液和未黏附细胞，加入含体积分数为0.1的胎牛血清、10 μg/L表皮生长因子及角质细胞无血清培养基的表皮干细胞培养液，细胞约70%~80%融合后传代扩增；将未黏附细胞接种于另一培养板中，相同条件下作为角质细胞对照培养。
In each subframe, 192 bits of word is processed in a single audio block, translating into a fraction of 192/8 = 24 bytes.
在每一副车架总成，192位元的字是在处理一个单一的音频块，转化成一小192/8 = 24字节。
The BED experiment for acetonitrile-toluene system with isopropyl benzene and mixed solvents was carried out respectively and the influence of reflux ratio and solvent ratio on BED process was analyzed in this paper. The result shows that the performence of mixed solvents is superior to single solvent and the molar fraction of acetonitrile in column top can reach 0.994; reflux ratio and solvent ratio have little influence on the yield of acetonitrile; reflux ratio can be 2~3 in favor of separation of acetonitrile and isopropyl benzene at stage of acetonitrile distillated; solvent ratio of mixed solvents at 1~1.5 is adequate; reflux ratio can be moderately decreased to reduce operational time at subsequent stage.
分别采用纯异丙苯及异丙苯-NMP混合溶剂进行了乙腈-甲苯物系的间歇萃取精馏实验并对回流比和溶剂比对过程的影响进行了分析，结果表明：在相同实验条件下，混合溶剂的分离效果明显好于单一溶剂，塔顶乙腈纯度可达0.994；溶剂比及回流比对乙腈收率的影响不大；在乙腈产品采出阶段，可取较大回流比以利于乙腈和异丙苯的分离，如2~3，同时混合溶剂的溶剂比不宜过大，可取1~1.5；在采出甲苯产品及回收溶剂阶段可适当降低回流比以减少操作时间。
It is extremely unlikely that drugs which target a single gene like Gleevec will be active against a major fraction of solid tumors.
单一的基因靶向药物如格列卫将对大多数实体肿瘤有效是绝对不可能的。
A median dose of 20 Gy prescribed to the 80% isodose line was administered in a single fraction.
中位值为20戈瑞剂量（建议达80%的等剂量线）以单成分方式给予患者。
Numerical simulation and performance analysis of flow fields in hypersonic low temperature nozzle and optical cavity of a small continuous wave DF chemical laser, which employed a single throat HYLTE nozzle, under different stagnation and structural parameters have been carried into execution to interprete the disparity between device design and experiment. Distribution of F atom mass fraction, static pressure and static temperature in cold flow field has been analyzed.
针对一台单喉道的小型连续波DF化学激光器设计指标与实验结果的差别，利用数值方法对不同条件的喷管光腔冷流场进行模拟和诊断，分析了光腔中的F原子质量百分比浓度、静压和静温分布以及反应流场小信号增益系数分布，并与实验光斑定性地进行了比较，取得与实验观察一致的结果，从而为该小型DF激光器的改进措施确定提供了理论依据。
According to the idea of Kelessidis (1989) for the flow pattern transition from churn to annular flow and the integral analysis method of Wallis (1969) for the velocity profile of single-phase flow in an annulus, a new model for prediction of void fraction and pressure drop is proposed based on considering the structural feature of the concentric annulus and the flow pattern characteristics.
根据Kelessidis有关团状流向环状流转换的思路以及Wallis有关圆管内环状流积分分析的方法，考虑环形管的结构特徵及环状流的流型特徵，通过两相动力学理论建立了新的预测截面含气率及总压降的模型。
In order to study the bubbling characteristic of single bubble cap in stagnant liquid, double-sensor conductivity probe has been used to determine the phase interfacial parameters such as local void fraction, interfacial area concentration and mean bubble size at the conditions with different gas volume flux and different height.
为了研究泡罩在静止液体中的鼓泡特性，用双头电导探针测试了不同气量下不同轴向位置处的相界面参数——局部含气率、界面浓度、气泡平均尺寸，得出了它们沿径向的分布规律：在轴向高度略高于泡罩高度处，局部含气率呈中间峰值分布形态，在径向r/R=0。
The optimal fraction x of Ge in GeSi layer defined by the necessity of single mode, numerical aperture and critical thickness is between 1～3%.
GeSi合金层中Ge的含量x要满足脊形光波导是单模、光波导的数值、孔径接近单模光纤值、脊高小于临界厚度值等，计算表明兼顾上述三项要求应取x＝1～3％；脊的高与宽受大断面及单模的制约。
Multiple-walled carbon Nanotubes were used as carrier to load cerous sulfate catalyst for catalytic synthesis of n-butyl propionate Single factor and orthogonal experiment methods were applied to investigate the effects of the molar ratio of butyl alcohol to propionic acid, cerous sulfate mass fraction and reaction time on the conversion of propionic acid and the optimal technical conditions were determined as:ratio of butyl alchhol and propionic acid 1.5:1, cerous sulfate mass fraction 15% and reaction time 70 min.
以多壁碳纳米管作载体担载硫酸铈催化合成丙酸丁酯。分别采用单因素和正交实验方法考察了醇酸摩尔比、硫酸铈质量分率和反应时间等主要因素对丙酸丁酯得率的影响，并确定了最佳工艺条件为：醇酸摩尔比1.5：1、硫酸铈质量分率15%和反应时间70 min。
Firstly, in my paper, the equipment of batch distillation was used, raw material from the sourse of natural gas which had high quality relatively was chosen to make high purity methanol through analyzing the testing results of three different sources of methanol inland; Secondly, constant-molar-holdup model of batch distillation for high purity methanol was proposed and solved by the two-point implicit combined with component balance calibration. Some kinds of effect factors on recovery fraction of high purity methanol were simulated, such as reflux retio, theoretical plates stages, holdup slop, cut withdrawal, etc. As the simulating results showing, when the composition of raw material was 98%, and the average purity specification of high purity in top of column was above 99.9%, the feasible number of theoretical plates was 35, the reflux retio of product phase and slop cut withdrawal respectively were 5 and 10, and the proportion of the outpu of slop cut withdrawal was 15%. Accordingly, the single recovery fraction of high purity methanol was between 50% and 65%. Thirdly, in order to testify the veracity and reliability of the model mentioned above, the raw material of methanol from natural gas was used to experiment for the process of batch distillation. The experimental result was relatively closed to the simulating result., the scope of error was below 10%, which showed it preferable veracious and feasible that constant-molar-holdup model was used to simulate the process of batch distillation for high purity methnol.
本文首先利用间歇精馏装置，分析国内三种不同甲醇原料气来源的甲醇原料，比较结果，最终选择较优的天然气为原料气的甲醇原料制备高纯甲醇；其次，建立高纯甲醇的间歇精馏恒摩尔持液模型，采用了两点隐含法和组分衡算相结合的方法对该模型进行了求解，模拟分析了影响高纯甲醇收率的各种因素，如回流比、理论板、持液量和前馏分等，计算结果表明：当原料组成为98%，塔顶甲醇产品的平均浓度≥99.9%时，适宜理论板数为35块，产品段回流比R为5，前馏分段回流比R_1为10，前馏分的馏出量控制在15%，所得到高纯甲醇的单程收率在50～65%之间；随后，实验验证了模型的准确性和可靠性，实验结果与模型计算结果比较吻合，误差范围在10%以下，表明本文所建的恒摩尔持液模型用于高纯甲醇的间歇精馏模拟计算具有较好的准确性和可靠性；最后，在实验与模拟结果的基础上，确定了高纯甲醇的最终制备工艺路线：理论板数34块，回流比R为5，前馏分段回流比R_1为10，前馏分的馏出量为15%，最终产品单程收率是57.1%。
Objective: To explore the pain relief effect with a single fraction and multifraction radiotherapy for bone metastases.
目的：研究单次放射治疗与多次分割放射治疗对于骨转移性疼痛止痛效果。
The model is based on data obtained from a single zone heat release rate analysis coupled with appropriate soot formation and oxidation models for evaluation of the radiation and burned zone temperatures, the soot volume fraction, the soot emissivity and the global radiative heat flux.
该模型利用单区放热率所得资料，配合适当的碳粒形成和氧化的子模型，以计算辐射温度，燃烧区温度，碳粒浓度，碳粒发射率和总体辐射热流量等。
Based on the orthogonal design, this paper has investigated the effects of four technological parameters (final rolling temperature, degree of final deformation, quenching temperature, cooling time after final rolling) on martensite volume fraction of hot rolled dual phase steel in single sheet.
在正交实验的基础上，采用方差分析方法研究了4个工艺参数（终轧温度、终轧变形量、淬火温度、轧后缓冷时间）对单张热轧双相钢马氏体体积分数的影响。
For single fraction radiosurgery, a clear correlation has been demonstrated between the target size and the risk of adverse events.
在单次分割的放射外科中，在靶区体积与反应事件发生率之间有明确的相关。
Objective To compare the relative accuracy of single-plane and biplane area-length methods for determining right ventricular (RV) volume and ejection fraction (RVEF) both in vitro and in vivo studies.
目的比较单平面和双平面面积-长度法计算右心室容积和射血分数的相对准确性。
Intra-operative radiation therapy (IORT) is defined as both the tumor bed and residual tumor organization are irradiated by a large single fraction dose under direct vision in surgical resection.
术中放疗是指在手术切除肿瘤后直视下用射线对瘤床及可能残留的肿瘤组织进行单次大剂量放射治疗。
The figure represents only a fraction of WPP's total sales of roughly $ 12bn a year, but it trumps lobbying fees generated by two large law firms in town that are the largest single-brand lobby shops in Washington: Patton Boggs and Akin Gump.
这仅占WPP每年约120亿美元总销售额的一小部分，但超过了华盛顿两家大型律师事务所PattonBoggs和AkinGump的游说费用。这两家律师事务所是华盛顿最大的单一品牌游说机构。
Results show that the volume fraction influences the distributions of coordination number of single contact type and individual components.
结果表明，单一接触类型的配位数及分量配位数分布与体积组成有关。
Modified CO 2 as mobile phase is usually necessary for packed column supercritical fluid chromatography. Single syringe pump was applied in this work to add modifier through a parallel connection device, which can maintain constant and stable volume fraction of modifier without any contamination of the pump.
采用单个注射泵，以并联流路装置，在超临界CO2流体中在线添加甲醇等改性剂，用作填充柱超临界流体色谱的流动相。