Differentiation of secondarystructure of DNA between the two subgenera was mainly focused on theirsecond hairpin structures.
两个亚属间RNA次级结构差异主要集中在三个稳定发夹结构中的第二个。
Specific primers were designed according to published nucleotide sequence of FABP gene in the GenBank database.
摘要 根据GenBank中细粒棘球蚴脂肪酸结合蛋白基因cDNA序列设计引物，并在起始密码子前加上Kozak 序列，提取细粒棘球蚴的原头蚴总RNA，RT-PCR扩增目的基因。
RNA only was separated successfully from the seeds by the methods of CTAB.
结果发现：用SDS法、上海华瞬植物叶RNA抽提试剂盒都没有成功提取RNA，只有CTAB法成功地提取出了完整的甘蓝型油菜籽粒RNA。
Based on the methods of CTAB, SDS and Plant Total RNA Isolation Kit, total RNA was isolated from seeds of 40 days of flower from Braccica napus.
用CTAB法、SDS法、上海华瞬植物叶RNA抽提试剂盒分别提取甘蓝型油菜花后40d的籽粒RNA。
Like other biopolymers, the functions of RNA are dependent upon their structures.
如同生物体中的其它大分子一样，RNA的功能取决於它们的结构。
In nature, this base is found in RNA, rather than DNA, but it is just as susceptible to being recorded by one of Dr Watson`s mechanical monkeys as the others are.
我们可以在RNA，而不是DNA里找到这种尿嘧啶基数。不过，它就象沃森博士的一个猴子机械化地记录下来的结果一样的容易受动摇。
The lac repressor The lacI gene has its own promoter to which RNA polymerase binds and initias transcription.
Lac阻遏物 LacI基因有自身的启动子，能够和RNA聚合酶结合起始转录。
Keywords: Human embryonic stem cells; EBV episomal vectors; Stable transgene transfer/expression; RNA interference
中文关键词：人胚胎干细胞；EBV游离体载体；稳定基因转染/表达；RNA干扰
In order to examine the role of astacene on mice body development and the expression of energy metabolism related genes in mice, we treated mice and primary culture of mouse muscle cells with astacene of higher and lower concentration. Then the total mRNA was extracted from the muscle tissue and cells respectively, and the mRNA levels of UCP3 and LXRα were detected by RT-PCR in all the samples.
本试验用高、低浓度虾红素日粮饲喂昆白系小鼠和处理原代培养小鼠骨骼肌细胞，提取总RNA，检测各时段UCP3、LXRa基因mRNA表达量，探讨虾红素对小鼠个体发育、肌肉能量代谢相关基因表达变化规律的影响。
Although miRNAs represent a vital component of the innate antiviral immune response in plants and invertebrate animals, it is not clear whether they play the similar roles in the defense of viral infection in mammalian organisms. Hepatitis C virus is the only member of the hepacivirus genus in Flaviviridiae. It contains a 9.6 kb single–stranded RNA genome with positive polarity.
在植物及非脊椎动物中，miRNA是先天性抗病毒免疫反应占有重要一环，但仍不清楚miRNA在哺乳动物中是否也扮演类似的角色。C型肝炎病毒（hepatitis C virus，HCV）是隶属於黄病毒科的唯一成员，其病毒基因体为一条长度约9.6 kb的正股RNA病毒。
Extensive findings suggest that heterogeneous nuclear ribonucleoprotein K is a multi-function RNA/DNA-binding protein in hnRNP family. Many research has shown that there is over expression of hnRNP K in multiplicate tumour such as rectum cancer and galactophore cancer.
大量的研究表明hnRNP K是hnRNP家族中的一种多功能蛋白，在直肠癌及乳腺癌等多种肿瘤中均有高水平的表达，但在肺癌发生、发展中的作用尚不清楚。
The situation that several coding proteins which are M2 ion channel, neuraminidase, hemagglutinins and RNA polymerase play important roles in replication cycle of avian influenza is approved by molecular biology.
分子生物学研究表明，在流感病毒的传染复制过程中，一系列的编码蛋白如M2离子通道、神经氨酸酶、血细胞凝集素以及RNA聚合酶等起着举足轻重的作用。
In the test the RNA/DNA ratio changed little between the three groups, while in the ovary the RNA/DNA ratio in the TBTO groups obviously decreased compared with that in the control group.
TBTO 暴露组的RNA/DNA比在精巢中无明显变化，但在卵巢中显著低于对照组。
He protein and vitellin content of the ovary in the TBTO groups showed similar changes in the RNA/DNA ratio of the ovary, indicating that the accumulation of TBTO in the ovary inhibited the synthetic activity of vitellin.
BTO暴露组的卵巢中蛋白质和卵黄蛋白含量显示了与RNA/DNA比相同的变化趋势，表明TBTO的积累阻碍卵黄蛋白的合成。
An enzyme that catalyzes the formation of DNA and RNA from an existing strand of DNA or RNA.
一种催化DNA或RNA的酶，利用一段既成的DNA或RNA作为核酸的模子。
Also, experiments were carried out to investigate the interactions between BRs, cytokinin and polyamine in cucumber fruit.
研究了BRs在果实发育中的作用和分子依据，在此基础上，研究了BRs对幼果RNA转录本的影响。
Brinkmann U%Mattes RE%Buckel P High-level Expression of Recombinant Genes in Escherichia coli is Dependent on the Availability of the dnaY Gene Product 10.1016/0378-1119(89)90470-8 Gene, 1989, 1
隋广超%刘芳%胡美浩利用噬菌体T7RNA聚合酶在大肠杆菌中引导尿激酶原克隆基因的表达北京大学学报，1994，6
To assess the protective effect of recombinant gam56 against E. maxima infection in chickens, gam56 gene of E. maxima was amplified by RT-PCR from total RNA of purified gametocytes. Two fragments with rich antigenic epitopes were subcloned into pGEX-6P-1 and expressed. Five-day old chickens were subcutaneously immunized with soluble GST-gam56-2 antigen with or without adjuvant, and boosted with the same dosage at 12, 19 days of age.
为评价gam56基因重组表达产物作为亚单位疫苗预防鸡巨型艾美球虫感染的效果，以E.maxima NT株配子体总RNA为模板，RT-PCR扩增和克隆gam56基因，选择该基因两段含丰富抗原表位的编码区片段，利用原核表达载体pGEX-6P-1在大肠杆菌中进行截短表达。
Cepacia containing T7 RNA. After shake flask culture, we found B.
cepacia宿主菌中，最终得到一系列脂肪酶基因工程菌。
A virion consists of a protein coat surrounding one or more strands of DNA or RNA.
一个病毒粒子包括外部的蛋白衣壳和内部的一条至多条 DNA 或 RNA 链。
The RNA tests target genetic evidence of HIV and can detect early infections which the current antibody screening fails to spot.
It has developed and industrialized production of micro RNA drugs, nucleotide and antitrope drugs.
Kornberg described how information is taken from genes and converted to molecules called messenger RNA.
Low levels of the mutant RNA dramatically decreased growth rates of breast and prostate cancer cells in the study and made more cells die.
The RNA polymerase is used to make copies of the virus once it has entered a host cell.
" We got part of its RNA sequence from Britain's Health Protection Agency, " he said.
Conducting RNA tests on all the samples may require another 26 million yuan annually.
The genetic screening tests for RNA could be used at blood centers across the Chinese mainland.
Mello for discovering RNA interference, a process that can silence specific genes.
This little bit of genetic material closely mimics a stretch of human RNA, they reported in the journal Nature Structural and Molecular Biology.