A cluster analysis of two-hundred candidate wheat cultivars from Shaanxi, Gansu, Sichuan and Henan provinces recently was performed by using unweighted pair-group average method, based on the data from their seedling resistance reaction to six dominant isolates of Puccinia striiformis with the different virulence.
为了弄清我国小麦条锈病主要流行区后备小麦品种的抗锈性，选用6个条锈菌优势菌系，对陕，甘，川，豫四省近年来培育的200份后备小麦品种进行苗期和成株期鉴定，并分别对不同省份后备品种的抗条锈性进行聚类分析。
To detection of Tilletia controversa Kühn sensitively and accurately, real-time PCR systems were developed. The species-specific primer pair CQUTCK4/CQUTCK5 and probe CQUP1 were designed based on a selected specific fragment (1322 bp) specific for TCK, and the SYBR Green I and TaqMan quantitative PCR detection systems were established with optimized reaction conditions.
建立荧光定量PCR体系以准确灵敏的鉴定小麦黑穗菌(Tilletia controversa Kühn，TCK)根据筛选的TCK独有差异基因片段(1322bp)设计特异性引物对CQUTCK4/CQUTCK5和TaqMan探针CQUP1，建立SYBR GreenⅠ荧光染料法和Taq Man水解探针法定量PCR检测体系，并对体系进行优化。
The details are summarized as follows: 1.γ-irradiation is extended to control the morphology of microcrystals. In our experiments, copper acetate aqueous solution, a simple reaction system, is used at room temperature to prepare pure hexapod-shaped Cu〓O microcrystals, in which a buffer pair is in-situ formed to adjust the acidity of the reaction solution. Some important information on intermediate is obtained by changing the reaction parameter, which favors to reveal the inherent growth habit of Cu〓O.
发展了γ-射线辐照合成法对微晶的形貌的控制，在常温常压下从一个简单的反应体系——醋酸铜水溶液出发，通过辐照过程中原位产生的醋酸-醋酸根缓冲对的自发调节作用，控制反应体系的酸度从而合成得到了纯相的六足状氧化亚铜微晶；并通过改变反应参数，获得了中间体的信息，从而为揭示氧化亚铜的固有生长习性提供了条件。
Reaction mechenism between heteropoly acid and organic matter is generation of electron-hole pair and producing of three radical such as.
杂多酸与有机物反应的机理主要是电子-空穴对的产生和。
A pair of primers were designed according to the conservative regions of the plant floral meristem identity gene-APETAL41 (AP1) homologous genes. In both loquat cultivar Xiangzhong 11 and wild variety Liye, a fragment about 350 bp of AP1 homologous gene was amplified respectively by polymerase chain reaction using the genomic DNA. The fragments were cloned into pUCm-T vector, and then sequenced.
分析植物花分生组织特征基因APETALA1(AP1)同源基因的保守区序列，设计特异引物；用PCR方法从枇杷栽培品种香钟11号和野生种栎叶枇杷基因组DNA中各扩增出1个350bp左右的片段；将该片段分别克隆到pUCm-T载体。
A pair of redox peaks attributed to the direct redox reaction of horseradish peroxidase in the phosphate buffer solution was observed.
结果显示：在磷酸缓冲溶液中，辣根过氧化物酶在钛酸纳米管修饰的玻碳电极上呈现出一对良好的氧化还原峰。
In this paper, importance of the lone pair electrons in molecular structure and chemical reaction is expounded by effect of the lone pair electrons.
本文通过对孤对电子效应的研究来阐明孤对电子在分子结构和化学反应上的重要性。
Analyse and compare what adopt a variety of pair of methods, the Michael addition reaction of ester of acerbity second of second of hydroxyl of protection of benzyl of the response of close nucleus addition that uses second nitrile and ester of formic acid second certainly, chloridize, cyanogen, reaction that take off carboxyl generates 3- benzyl oxygen radical the fifth of the ten Heavenly Stem the method of 2 nitrile, detailed discussion the action that wait and the by-product that arise possibly reach the reactant in each pace reaction, activator, dissolvent avoid a method.
通过对多种方法的分析和比较，确定采用乙腈和甲酸乙酯的亲核加成反应、氯化苄保护羟基、氰乙酸乙酯的Michael加成反应、脱羧反应生成3-苄氧基戊二腈的方法，并具体的讨论了每一步反应中反应物、催化剂、溶剂等的功能和可能产生的副产物及避免方法。
In the presence of catalytic amount of glacial acetic acid, a pair of chiral nonracemic vinyl monomers, -2, 5-di {4'-［ N -(S -α-methylbenzyliminemethylene］phenyl}styrene (M 1)and-2, 5-di {4'-［ N -(R -α-methylbenzyliminemethylene］phenyl}styrene (M 2), was synthesized through the condensation reaction between 2, 5-di(4'-formylphenyl)styrene and optically pure α-methylbenzylamine.
在催化剂量的冰乙酸存在下，与光学纯的--α-甲基苄胺或--α-甲基苄胺发生缩和反应，得到了一对手性非外消旋单体，-2，5-二{4'-［ N -(S-α-甲基苄亚胺基次甲基］苯基}苯乙烯和-2，5-二{4'-［ N -(R -α-甲基苄亚胺基次甲基］苯基}苯乙烯。
The results indicate that the flexibility of the first base pair can affect the editing reaction catalyzed by LeuRS.
这些结果表明tRNA〓接受茎上第一对碱基配对的柔性程度能够影响LeuRS的编校。
Ion-pair Complex-based Solvent Extraction Combined with Chemiluminescence Determination of Chlorpromazine Hydrochloride and Promazine Hydrochloride withReverse MicellesBased on the CL reaction between AuCLT carried by the ion-pair complexes formed by the chlorpromazine hydrochloride or promazine hydrochloride and luminol in a CTAB reversed micellar medium.
本法已成功用于片剂、针剂和生物样品中盐酸氯丙嗪与盐酸异丙嗪的分析。3。基于离子缔合物溶剂萃取化学发光测定盐酸小檗碱根据在酸性介质中盐酸小檗碱与AuCl_4~-形成离子缔合物，结合液-液萃取技术建立了一种简单、灵敏测定盐酸小檗碱的新方法。
Two cDNA fragments (SPVG-CPSC1, SPVG-CPSC2) with about 700bp were cloned from sweet potato Ipomoea batats (L. Lam. which were suspiciously infected with sweet potato virus by reverse transcription-polymerase chain reactionmethod using a pair of specific primers which were designed based on the conserved region of the coat protein genes of sweet potato virus G.
根据已报道的甘薯G病毒（Sweet potato virus G，SPVG）外壳蛋白基因（coat protein，CP）序列，设计和合成了一对特异引物，采用反转录-聚合酶链式反应（Reverse transcription-polymerase chain reaction，RT-PCR）技术，从甘薯病叶总RNA中克隆到了SPVG-CPSC1和SPVG-CPSC2两个约700bp的cDNA片段。
A friend invited me to Nepal, originally, I thought I would suffer from the reaction of high altitudes and ultraviolet irradiation, therefore, I prepared the Northland water proof and breathable equipment, OZARK down-filled sleeping bag, super anti-ultraviolet radiation sun-proof cream and classes except of snow glasses, and one thing is very important, it is a pair of Gore-Tex Garmont shoes.
去尼泊尔是我的一个朋友邀请我去的，开始我以为去那里要经历一些高山反映、紫外线的照射、所以准备了NORTHLAND的冲锋衣裤、OZARK的羽绒睡袋、高倍数的防紫外线的防晒霜、眼镜，只差没有买雪镜了。还有最重要的就是一双GORE-TEX GARMONT的鞋子。
Methods Streptococcus sanguis and streptococcus mutans were cultured in anaerobic atmosphere and the genome was obtained by the alkaline method. A pair of primers were designed in the most reserved region of the sequences from GenBank database in order to obtain the unique polymerase chain reaction products.
厌氧培养变形链球茵和血链球菌，碱裂解法提取细菌基因组，根据GenBank基因库上发表的各种属ffh基因序列，从最保守区设计一对聚合酶链反应引物，钓取基因，进行PCR。
What was more, for Vibrio alginolyticus, the PCR rapid detection kit was developed. The details were as follows: 1. The genomic DNA of Vibrio fluvialis, V. alginolyticus, V. anguillarum and V. mimicus were prepared by alkaline lysis with SDS. Then a pair of universal primers were used for hybridization to the genomic DNA by polymerase chain reaction.
具体内容如下：1、采用SDS裂解法提取了河流弧菌、溶藻弧菌、鳗弧菌、拟态弧菌共四种水产病原性弧菌的基因组DNA；利用gyrB基因的通用引物对以上四种弧菌的基因组DNA进行PCR扩增，结果均得到了长度为1200bp左右的目的片断，通过凝胶回收并转化感受态E.coli，经蓝白斑筛选后获得阳性克隆，经测序后获得了相应的DNA序列信息。
A pair of new redox peaks has been fOund at Ptelectrode in negative potential, Which is a quasi-reversible redox reaction witha one-electron transfer process. The intermediate of tertiary carbon freeradical exists in this process.
在Pt电极上负电位下一对未曾报道过的氧化还原峰被发现，这个氧化还原反应是发生一个电子的准可逆过程，叔碳自由基中间体在反应中产生。
In the reaction of 〓 with the diiron cationic bridging carbyne complexes, we obtained a number of novel trimetal bridging carbene complexes or ion-pair bridging carbyne complexes in high yields, while in the case of 〓 and 〓, no analogous trimetal bridging carbene complexes or ion-pair bridging carbyne complexes, but rather the diiron bridging arylcarbene complexes 〓 were obtained.
在同含氰基配体的羰基金属阴离子络合物的反应中，合成了一系列具有新型结构的异核三金属桥卡宾络合物和离子型多核金属桥卡拜络合物。这是合成异核三金属桥卡宾和桥卡拜络合物简便有效的方法。我们测定了代表络合物的晶体结构，进行了分子结构研究，并对反应机理进行了初步探讨。
Two pairs of SRY gene primer were designed as the nested primers for sex identification and one pair of HBB gene primer as an internal standard in this study and established the system of nested polymerase chain reaction.
根据牛 SRY基因序列设计合成了2对嵌套式引物作为公牛特异性引物，同时根据牛β珠蛋白基因序列设计了1对引物作为内参照引物，建立了巢式PCR反应体系。
Methods Twelve HCC and pair-matching adjacent nonneoplastic tissues, 10 liver tissues of HBV, 7 normal liver tissues specimens, taken directly from patients and confirmed by pathology section, and the cell lines BEL7402, SMMC7721, HepG2, L02 were subjected to analysis. According to the templat, genomic DNA extracted from HCC tissue specimens, the promoters of IGF-Ⅱ(P1-P4) were amplified by means of nested polymerase chain reaction and then sequencing. Restriction enzyme Hpa Ⅱ and Msp I were utilized to detect the methylation pattern of the IGF2 promoter. The genomic DNA extracted from tissue specimens and cell lines were modified by bisulfite sodium and determined by methylation specific PCR.
方法采集手术切除的肝癌及配对的癌旁肝组织12例、慢性乙型肝炎肝穿刺活检组织10例、正常肝组织7例，均经病理学证实；培养三株肝癌细胞系(BEL7402、SMMC7721、HepG2)及胎肝细胞系L02；提取基因组DNA为模板，巢式PCR扩增IGF-Ⅱ基因启动子(P1、P2、P3、P4)，肝癌组织IGF-Ⅱ启动子扩增产物测序；甲基化敏感的限制性内切酶HpaⅡ和不敏感的限制性内切酶MspⅠ消化基因组DNA，酶切产物为模板，扩增IGF-Ⅱ基因启动子；亚硫酸氢钠修饰基因组DNA，甲基化特异性PCR检测各启动子的甲基化状态。
A pair of intraluminal brachytherapy of malignant pleural mesothelioma have some reaction, and a few patients long-term results, it appears a glimmer of hope.
腔内放疗对少数恶性胸膜间皮瘤有某些反应，且少数病人长期疗效，看来有一线希望。