Cultures were employed. The purity of pericyte cultures was assessed morphologically and confirmed by staining for 3G5-defined ganglioside antigen and α-actin and factor Ⅷ antibody. Then we studied the effects of early glycation products and advanced glycation end products in different concentration on growth of retinal capillary pericytes by counting cell number on a counting plate and MTT colorimetric assay, and 〓 thymidine incorporating. Meanwhile, the effect of EG-BSA and AGE-BSA in the different concentration on cytosolic free calcium 〓 of pericytes was further studied by the fluorescent indicator fura-2 acetoxy-methy1 ester (Fura-2AM). The protective effectiveness of aminoguanidine、silybin、654-2 on the growth of pericytes cultured in the medium contained with EG-BSA and AGE-BSA (final concentration was 500μ g/ml) were estimated yet.
并通过对其形态和应用单克隆抗体3G5和α-平滑肌肌动蛋白抗体以及Ⅷ因子抗体染色进行鉴定，然后，采用细胞计数结合MTT比色测定，以及〓胸苷嘧啶掺入和钙荧光探剂Fura-2AM，研究不同浓度的早期糖基化产物（Early glycation products of albunin，EG-BSA）和糖基化终产物（adavaced glycation end products ofalbunin，AGE-BSA），对培养的牛视网膜毛细血管周细胞增殖和胞浆内游离钙离子浓度的影响；并观察不同浓度的糖基化产物抑制剂-氨基胍、水飞蓟素及抗氧化剂山莨菪碱（654-2），对在EG-BSA和AGE-BSA培养条件下（终浓度：500μg／ml）对周细胞增殖和胞浆i浓度改变的保护作用。
In the paper, the processes for extractions of cholesterol from crude lanolin and productions of lanolin alcohol and lanolin acid as byproducts were studied, and the processes of saponification resultants separation, complexing of cholesterol and crude cholesterol recrystallizing were focused on. The effects of every factor on yield and purity of products, and the suited process conditions were presented.
本文研究了以粗羊毛脂为原料提取胆甾醇及副产羊毛酸和羊毛脂醇的工艺过程，着重研究了粗羊毛脂皂化产物的分离、胆甾醇配合及粗胆甾醇提纯的过程，得出了各影响因素对胆甾醇纯度及收率、羊毛脂醇产率、羊毛酸收率的影响规律和相应的适宜工艺条件。
Energy may be sublimated through creative art, music, or drama; work may be found dealing with the healing professions; devotion to human welfare in some form could be a splendid corrective measure in redirecting the desire nature; knowledge and understanding of sex, physiologically, psychologically, and esoterically, would certainly serve to clarify the mental pictures so that the person could, to greater degree, understand his problem; establishment of cleanliness as a factor in his physical habits could bring his consciousness into attunement with the ideal of inner and outer purity; and, above all, establishing in the consciousness the goal of love by which all desire impulses find their regeneration.
最好能通过艺术创作，音乐，戏剧来使能量得以升华，选择有关医疗方面的工作，致力于公共福利事业会将欲望引导至正确的方向上。加深对性的认识和理解，在生理上心理上都会潜在地有助于认识自己所存在的问题。养成一种洁净的性方式，作为一种生理习惯因素，它会有助于意识与内外在纯洁的理想形成和谐的统一，更重要的是，将爱的理想植根于意识之中，借此使冲动和欲望得以净化重生。
OBJECTIVE:To de termine protein content, protein molecular weigh and purity of recombinant human granulocyte colony stimulating factor.
钟英目的：测定重组人粒细胞集落刺激因子的蛋白浓度、分子量及纯度。
In the process of cucumber seed production, the false hybrids formed by maternal self and sister across often bring a great loss to cucumber production, and the mixture of different varieties of cucumber hybrid seed is one of the most important factor to reduce the hybrids purity.
黄瓜制种过程中，除了母本自交和姊妹交形成的假杂种经常给黄瓜生产造成损失外，不同黄瓜品种种子的混杂也是造成杂交种子纯度降低的一个重要因素。
The fourth factor was mainly dominated by apparent purity and gravity purity determining sugarcane maturity.
第4主因子起支配作用是重力纯度和视纯度等衡量甘蔗成熟度的因子。
The ionic liquid [BMIm] Br was prepared with N-methyl imidazole in microwave radiation instrument. The best preparation conditions were determined through the single-factor experiment and temperature variation. The structure and purity of the ionic liquid were also characterized by IR survey in this article.
本文以N-甲基咪唑为原料，利用微波反应仪合成溴代3-丁基-1-甲基咪唑盐[BMIm]Br，通过考察体系温度的变化及单因素实验确定了最佳制备条件，并通过红外光谱表征了结构和纯度。
I Nerve growth factor (125 I NGF) with more than 95% of radiochemical purity is prepared by chloramine T method.
用氯胺T法制备了125I神经生长因子（125INGF），放化纯度大于95%。
Regenerant purity is the main factor affecting the purity of make-up water quality in power-plant provided that other productive conditions are determined.
电厂补给水处理在其它生产条件相对稳定的状况下，再生剂纯度是影响水质的主要因素。
At the present, customer have higher and higher requirement for resolution and color purity of CRT. As one of the most important factor of influencing purity, the electronic beam landing error is concerned.
目前，对彩色显示管的分辨率和色纯的要求越来越高，而电子束的着屏（Landing）误差是影响彩色显示管的色纯的关键因素之一。
Through orthogonal experiment optimization analysis and significant factor analysis, we preliminary got the effects of process conditions on chlorine dioxide bleaching solution: the mole rate of sodium chlorate and hydrochloric acid mostly affected the purity of chlorine dioxide and temperature took second place.
通过正交实验最优化分析和显著因子分析，初步得到工艺条件二氧化氯溶液的影响，由正交实验的极差分析和方差分析可知，氯酸钠与盐酸的摩尔比对二氧化氯纯度影响最大，温度的影响次之。
The Correlation Between Separation Factor and Purity and Preparation of High Purity Lanthana
分离系数与纯度的关系及高纯氧化镧的制备
The growth of neurons added cytosine arabinoside and nerve growth factor was well, the purity and survival rate of neurons were high.
加NGF培养的神经元生长良好，纯度和存活率均较高。
The factor of concentration was 25 and purity was above 99.98% by continuous operation, included removal of biomass from fermented broth, diafiltration, and concentration.
实现了除菌、洗菌、浓缩过程连续化操作，浓缩倍数达到25倍。
OBJECTIVE To produce factor ⅸ(F ⅸ) complex concentrates with high purity and low potential thrombogenicity.
目的制备纯度较高，潜在血栓性小的凝血因子Ⅸ（FⅨ）复合物。
OBJECTIVE: To determine protein content, protein molecular weigh and purity of recombinant human granulocyte colony stimulating factor (rhG CSF).
目的：测定重组人粒细胞集落刺激因子（rhGCSF）的蛋白浓度、分子量及纯度。
Results The relative molecular weight, specific activity and electrophoretic purity of the purified bovine nerve growth factor were 30000, 400000BU/rag and above 95% respectively, and a single peak was observed on the FPLC profile of it.
结果获得了纯的牛神经生长因子，相对分子质量为30000，比活性为400000BU/mg，FPLC检测结果为一个峰，电泳检测纯度大于95%。
The main influence factor of hybrid seed purity identification is the false hybrids from female parent self-cross.
影响番茄商品杂交种子纯度的主要原因是母本自交产生的假杂种。