The ethanol extract was dissolved with HCl, the acidic extract was basified to pH9-10 with NH_3-water and extracted with CHCl_3 to give total alkaloids. five alkaloids were isolated with column chromatography and preparative thin-layer chromatography, and three of them were identified as dehydrocorydaline、corynoline and dihydrosanguinarine on the basis of spectral analysis MS, ~1H-NMR, ~(13C-NMR and physicochemical properties. All of them were found for the first time from this plant.
为研究灰绿黄堇的化学成分，本文将灰绿黄堇乙醇提取物采取酸提取、碱游离后萃取的方法得到总生物碱，然后利用柱色谱、制备薄层色谱等方法分离、纯化了灰绿黄堇中5种生物碱，并利用IR、MS、~1H-NMR、~(13)C-NMR等方法鉴定了其中的3种，分别为去氢紫堇碱、紫堇灵、二氢血根碱，均为首次在此植物中发现。
This paper introduces tests of constituents and isomeric compound contents of PXE、PEPE and M/DBT produced by several chemical plant by way of gas chromatography and gas chromatography-mass spectrograph combination, and also gibes some comments on present status and quality control of the insulation oil.
本文介绍了采用气相色谱分析及气质联用分析方法对各厂家生产的PXE、PEPE、M/DBT的组份及其同分异构体的含量进行测定的情况，对绝缘油品质的现状及质量的控制提出了一些见解。关键词：绝缘油；气相色谱；组分分析；同分异构体
So it may be an active potential medicine for the treatment and protection against hepttic fibrosis and carcinoma. In this thesis, we examined the chemical constituents of this plant. Three secoiridoid glucosides, oleuropein, 10-hydroxy oleuropein (2), oleoside-11- methyl ester(3) and 2-(3, 4-dihydroxyphenyl) ethanol (4) were isolated by column chromatography on silica gel and TLC.
本论文通过硅胶柱层析并结合薄层层析，从小叶丁香的原植物中分离得到了裂环环烯醚萜甙类化合物——橄榄苦甙、10-羟基橄榄苦甙(10-hydroxyoleuropein)、oleoside-11-methyl ester和芳醇类的2-(3，4-二羟基苯基)乙醇[2-(3，4-dihydroxyphenyl)ethanol]，以及黄酮类化合物。
As little studying, the exploiture of Dracaena cambodiana Pierre ex Gagnep is restricted at present, the using of Dracaena cambodiana Pierre ex Gagnep which is a rare kind of plant resource is also restricted. The alcohol extractive technique of Sanguis draxonis from Dracaena cambodiana Pierre ex Gagnep has been investigated. The content of Loureirin B has been chosen as criteria, experiment, temperature, concentration of ethanol, multiple of solvent, time of extraction have been selected in the Orthogonal. HPLC is used to measure the content of Loureirin B in the extraction. The best extractive condition is: 20-fold of 85% alcohol for 1.5 hours and 60 ℃ By using CO2 supercritical fluid extraction to extract the effective components and gas chromatography-mass spectrum to do the analysis, 48 effective components of Dracaena cambodiana have been separated and authenticated. There are 41.93% aromatic compounds, 13.38% aliphatic compounds, 10.3% esters and carbonyl group compounds. With the best extractive condition, Sanguis draxonis is extracted from Dracaena cambodiana Pierre ex Gagnep. The Sanguis draxonis is detached into four parts by petroleum ether, acetic ether, n-butyl alcohol. The activity of bacteriostasis of the four partys are analyzed with Staphylococcu, Escherichiacoli, P. aeruginosa and Candidaalbicans. The partys from petroleum ether, acetic ether, n-butyl alcohol have obvious bacteriostatic activity to Staphylococcu, Escherichiacoli, P. aeruginosa, but no bacteriostatic activity to Candidaalbicans. The bacteriostatic activity of extract of acetic ether is better than others.
目前对海南龙血树的研究尚未深入，很大程度上制约了其药用价值开发，限制了这种珍贵植物资源的开发利用 1、海南龙血树树干超临界CO2萃取成分分析研究以甲醇为夹带剂，用超临界CO2仪萃取海南龙血树树干粉末，萃取物用GC-MS分析，初步定性了其中的48个成分，主要包括芳香族化合物(41.93%)、脂肪族化合物(13.38%)、酯类及羰基类化合物(10.3%) 2、海南龙血树树干血竭提取条件研究设计了温度，乙醇浓度，物料比，提取时间为因素的正交实验，以龙血素B为指标，用高效液相色谱检测海南龙血树树干提取物中龙血素B的含量，最优提取件为：过20目筛网的海南龙血树树干粉末在60 ℃下用85%乙醇，溶剂与物料比为20 ml/g，回流提取1.5 h；最佳条件下提取的血竭中龙血素B的含量为271.78 mg/ kg 3、海南龙血树树干粗提物抑菌活性研究在最佳提取条件下，提取海南龙血树血竭，依次用石油醚、乙酸乙酯、正丁醇萃取，将血竭分为四个部份，及石油醚部份，乙酸乙酯部份，正丁醇部份和剩余部份；分别以金黄色葡萄球菌、大肠杆菌、绿脓杆菌、白色念珠菌为供试菌种，对上述四个萃取部份进行生长抑制活性实验，其中石油醚、乙酸乙酯、正丁醇萃取部对金黄色葡萄球菌、大肠杆菌、绿脓杆菌均有明显的抑制作用，但对白色念珠菌没有表现出生长抑制作用；剩余部份对四种供试菌均没有抑制作用乙酸乙酯萃取部份的抑菌活性相对石油醚萃取部份、正丁醇部份较强，其抑菌活性对温度、pH值、紫外光照射和金属离子均稳定。
In this paper, the contents of di-n-butyl phthalate, nonylphenol and bisphenol A in the tailwater, filtered water, depsitted water, raw water of Huyan water plant and the drinking water sample of Shanxi University have been determined by the method of gas chromatography-mass spectrometry and gas chromatography. The resultes indicated that the three chemicals present in all the water samples.
本文选取三种已被确认的环境内分泌干扰物邻苯二甲酸二丁酯、壬基酚和双酚A为研究对象，分别采用气相色谱-质谱联用法和气相色谱法，对呼延水厂出厂水、过滤后水、沉淀后水、原水及山大水样进行分析。
The antifungal activities of these materials were investigated and build foundation to develop low poison plant antifungal products. In experiment, polyphenols were extracted by reflux ectraction method which applyed petroleum ether to degrease and methyl alcohol solvent. Phenolic hydroxyl were examined and inspected by colour-developing reaction and the content of total polyphenols was determined by UV-spectrophotometry. Screening test were performed to the antifungal activities of polyphenol extraction by the tube dilution method to the selected ten pathogenic fungus such as Dothiorella gregaria, Rhizoctonia solani, Phyllosticta pirina, and the minimal inhibitory concentration were measured. The column chromatography and thin layer chromatography were applyed to separate and analyse cortex extracts of Dracaena cochinchinensi Lour.
实验部分采用以石油醚脱脂，甲醇为溶剂的回流提取法来提取植物多酚，用显色反应检识酚羟基；采用紫外分光光度法对多酚粗体物进行总多酚含量的测定；选取了聚生小穴壳菌、立枯丝核菌、梨叶点霉等10株常见农林业致病真菌，采用试管液基稀释法对多酚粗提取物进行了抗真菌活性筛选试验，并测定其最低抑菌浓度；采用柱层析法和薄层层析法对其中抑菌效果较好的龙血竭进行分离分析，根据其中3种化合物的光谱数据UV、IR、~(13C-NMR、1H-NMR和MS鉴定其结构。
In this pilot plant study the author has generally studied the biodegradable characteristics of the micro polluted raw water through combination of various water standard items analysis with Gas Chromatography/Mass Spectrometer, as well as mutagical evaluation. It has been systematically studied how organic matter varied in each process effluent and how each process affected on water mutagenicity. At the same time, it has been studied on how various coagulants affected on water mutagenicity. Through comprehensive analysis and evaluation, the optimum scope of each process applied has been proposed. The pilot study showed that:(1) The conventional water treatment process which used alum sulphate and ferric chloride as coagulants resulted increasing of water mutagenicity.
本试验研究利用多种水质指标，结合色谱—质谱有机物分析技术及毒理学评价，综合分析了微污染水源水中有机物的生物降解性能；系统地研究了微污染水源水及各处理工艺出水中有机物的变化规律，以及各处理工艺对水的致突变活性的影响；同时，考察了不同混凝剂对水的致突变活性的影响；并且，通过对微污染水处理工艺去除水中微量有机物能力的综合分析、评价，提出了各组合工艺的适用范围。
After purified with chromatography column, the products of RT-PCR was cloned into T-vector and sub-cloned into Nco Ⅰ/BstE Ⅱ sites of the recipient plasmid pCAMBIA1301 which was digested with the restriction endonucleases Nco Ⅰ/BstE Ⅱ, and therefore a plant expression vector named pCA, was constructed.
采用RT-PCR法从人胎盘组织克隆Arresten基因cDNA，克隆产物经酶切后定向插入到植物双元表达载体pCAMBIA1301的Nco Ⅰ和BstEⅡ位点之间，构建成含有目的基因的植物表达载体pCA，经PCR、限制性内切酶检测及序列测定正确后，转化根癌农杆菌LBA4404，获得携带目的基因的重组农杆菌。
The yield, chemical components and relative contents of essential oil extracted from Eupatorium adenophorum Spreng by Soxhlet extraction and steam distillation were compared. The volatile chemical constituents of essential soil extracted were analyzed by Gas Chromatography-Mass Spetrometry. The results showed that the constituents of the volatile from the same plant and the relative contents were significantly different due to different extraction methods.
采用索氏提取器及水蒸气蒸馏两种方法对采于不同时间、不同产地的紫茎泽兰Eupatorium adenophorum Spreng挥发性成分进行了提取，比较了两种方法的得油率、化学组成及其相对含量；采用GC-MS联用技术对其中的挥发性成分进行了分析。
A method was established for the determination of bifenthrin residue in 8 plant-based foods using gas chromatography-mass spectrometry.
建立了气相色谱－质谱检测8种植物源性食品中联苯菊酯残留量的方法。
The residues of 20% triazophos EC in rice field water, plant, rice shell and unpolished rice were determined by gas chromatography.
用气相色谱法测定了田间试验中20%三唑磷乳油在稻田水、植株、土壤、稻壳和糙米中的残留。
Sitosterol and campesterol were successfully separated from mixed plant sterol standard by high speed countercurrent chromatography with V∶ V∶ V=5∶5∶1 solvent system.
利用高速逆流色谱法，并选用V∶V∶V =5∶5∶1溶剂体系，成功地分离了混合植物甾醇标准品中的β谷甾醇和菜油甾醇。
Guangzhou Zhengyang Trade Ltd. is a professional glass equipment operators, domestic and imported chemical reagents, biological reagents, laboratory equipment, chromatography consumables, chemicals and chemical equipment in the wholesale and retail enterprises, complete product specifications, types and physicochemical properties stability; In addition to operating in Germany Merck, the United States Sigma, the British OXIOD imported reagents, Switzerland Wantong Metrohm consumables, Shanghai Ltd. Sinopharm chemical reagents, chemical reagents No.2 Tianjin, Tianjin Damao reagents, Tianjin Glass Instrument Factory, North America glassy Bo BB glass, glass apparatus No.2 Yancheng, Shanghai Anting LAU Equipment Plant, Shanghai City-analysis equipment manufacturer products, and with a number of suppliers, manufacturers established a very broad spectrum of suppliers, forming a perfect domestic and imported high, midrange product line, but also to provide customers with various kinds of experiments, testing the scarcity of glass equipment, supplies, prices concessions.
广州市正阳贸易有限公司是一家专业经营玻璃仪器、国产和进口化学试剂、生物试剂、实验仪器、色谱易耗品、化工原料、化工设备的批发零售企业，产品规格齐全，品种多样，理化性能稳定；除经营德国Merck、美国Sigma、英国OXIOD等进口试剂、瑞士万通Metrohm易耗品、上海国药化学试剂有限公司、天津化学试剂二厂、天津大茂试剂厂、天津玻璃仪器厂、北玻博美玻璃仪器厂、盐城玻璃仪器二厂、上海安亭仪器设备厂、上海智城分析仪器制造公司产品外，并与多家供应、生产厂商建立了非常广泛的供货渠道，形成了完善的国产及进口高、中档产品系列，更可为客户提供各种实验、检验稀缺化玻仪器、耗材，价格优惠。
A quick method for analysing traces of abscisic acid in small plant samples with gas chromatography
用气相色谱法快速测定少量植物样品中脱落酸的方法
Determination of Phosmet in Plant Food by Gas Chromatography - Electron Capture Detection
植物性食品中亚胺硫磷的气相色谱&电子捕获检测器测定法
Analysis of the component forestry harmful plant of Ambrosia artemisiifolia essential oil by Gas Chromatography-Mass Spectrometry
林业有害植物豚草挥发油GC&MS成分分析
This paper describes two new methods about capillary gas chromatography to determine a plant hormone-indole-3-acetic acid (IAA).
本文介绍使用毛细管气相色谱测定植物生长素&吲哚-3-乙酸（IAA）含量的两个新方法。
P-polypeptide, that was so called plant insulin, was extracted from Momordica charantia seeds with vitriol and ethanol and purified with Sephadex G-50 gel filtration and RP-HPLC. The technique was better than thin-layer chromatography technology.
采用硫酸、乙醇等提取，SephadexG50及RPHPLC技术纯化，从苦瓜中分离出苦瓜多肽P，又称植物胰岛素，该技术优于过去的薄层层析纯化方法。
Plant insulin was extracted with organic acid and ethanol and purified with Sephadex G-50 gel filtration and reversed-phase high performance liquid chromatography (RP-HPLC) from Momordica charantia.
采用有机酸、醇提取，SephadexG-50、反相高效液相色谱技术纯化，从苦瓜中分离出植物胰岛素。
Study on Flavonoid in Plant Extracts from Plantago asiatica by Liquid Chromatography Electro-spray Ionization Mass Spectrometry
车前草中一种黄酮化合物的电喷雾多级串联质谱研究