High speed counter-current chromatography was successfully used for the isolation and purification of baicalein and chrysin from the extracts of Oroxylum indicum using stepwise elution with a pair of two-phase solvents systems composed of light petroleum-ethyl acetate-methanol-water (5:5:5:5 and 5:5:7:3, V/V).
建立了高速逆流色谱分离纯化木蝴蝶黄芩素和白杨黄素的方法。两相溶剂系统为石油醚－乙酸乙酯－甲醇－水，固定相为5：5：5：5体系的上相，以5：5：5：5和5：5：7：3体系的下相为流动相进行梯度洗脱。
As little studying, the exploiture of Dracaena cambodiana Pierre ex Gagnep is restricted at present, the using of Dracaena cambodiana Pierre ex Gagnep which is a rare kind of plant resource is also restricted. The alcohol extractive technique of Sanguis draxonis from Dracaena cambodiana Pierre ex Gagnep has been investigated. The content of Loureirin B has been chosen as criteria, experiment, temperature, concentration of ethanol, multiple of solvent, time of extraction have been selected in the Orthogonal. HPLC is used to measure the content of Loureirin B in the extraction. The best extractive condition is: 20-fold of 85% alcohol for 1.5 hours and 60 ℃ By using CO2 supercritical fluid extraction to extract the effective components and gas chromatography-mass spectrum to do the analysis, 48 effective components of Dracaena cambodiana have been separated and authenticated. There are 41.93% aromatic compounds, 13.38% aliphatic compounds, 10.3% esters and carbonyl group compounds. With the best extractive condition, Sanguis draxonis is extracted from Dracaena cambodiana Pierre ex Gagnep. The Sanguis draxonis is detached into four parts by petroleum ether, acetic ether, n-butyl alcohol. The activity of bacteriostasis of the four partys are analyzed with Staphylococcu, Escherichiacoli, P. aeruginosa and Candidaalbicans. The partys from petroleum ether, acetic ether, n-butyl alcohol have obvious bacteriostatic activity to Staphylococcu, Escherichiacoli, P. aeruginosa, but no bacteriostatic activity to Candidaalbicans. The bacteriostatic activity of extract of acetic ether is better than others.
目前对海南龙血树的研究尚未深入，很大程度上制约了其药用价值开发，限制了这种珍贵植物资源的开发利用 1、海南龙血树树干超临界CO2萃取成分分析研究以甲醇为夹带剂，用超临界CO2仪萃取海南龙血树树干粉末，萃取物用GC-MS分析，初步定性了其中的48个成分，主要包括芳香族化合物(41.93%)、脂肪族化合物(13.38%)、酯类及羰基类化合物(10.3%) 2、海南龙血树树干血竭提取条件研究设计了温度，乙醇浓度，物料比，提取时间为因素的正交实验，以龙血素B为指标，用高效液相色谱检测海南龙血树树干提取物中龙血素B的含量，最优提取件为：过20目筛网的海南龙血树树干粉末在60 ℃下用85%乙醇，溶剂与物料比为20 ml/g，回流提取1.5 h；最佳条件下提取的血竭中龙血素B的含量为271.78 mg/ kg 3、海南龙血树树干粗提物抑菌活性研究在最佳提取条件下，提取海南龙血树血竭，依次用石油醚、乙酸乙酯、正丁醇萃取，将血竭分为四个部份，及石油醚部份，乙酸乙酯部份，正丁醇部份和剩余部份；分别以金黄色葡萄球菌、大肠杆菌、绿脓杆菌、白色念珠菌为供试菌种，对上述四个萃取部份进行生长抑制活性实验，其中石油醚、乙酸乙酯、正丁醇萃取部对金黄色葡萄球菌、大肠杆菌、绿脓杆菌均有明显的抑制作用，但对白色念珠菌没有表现出生长抑制作用；剩余部份对四种供试菌均没有抑制作用乙酸乙酯萃取部份的抑菌活性相对石油醚萃取部份、正丁醇部份较强，其抑菌活性对温度、pH值、紫外光照射和金属离子均稳定。
The thermodynamics characteristics of aqueous inclusions reflect that stratum chromatography might exist. Hence, at least two petroleum migration periods should he compartmentalized. It is proved that the earlier stage originated in 90-50Ma (K2-E) and the later one in 50Ma which is the main period for oil migration and accumulation.
同期盐水包裹体的热力学特征反映研究区可能存在地层的层析作用，推断准噶尔盆地中部Ⅰ区块J1s储层的油气充注、运移至少经历两个主要期次，早期发生于90~50Ma(K2-E)，晚期发生于自50Ma至今，以晚期成藏为主。
Sulfur compounds in desulfurized liquefied petroleum gas were separated and analyzed by large _ bore capillary gas chromatography with flame ionization detector-double photometric detector as well as GC-MS.
利用大口径毛细管色谱柱、不分流进样、氢焰离子化检测器、双火焰光度检测器，与气相色谱-质谱联用技术相结合，对脱硫后的液化气进行测定，成功地鉴定出17种含硫化合物。
The antifungal activities of these materials were investigated and build foundation to develop low poison plant antifungal products. In experiment, polyphenols were extracted by reflux ectraction method which applyed petroleum ether to degrease and methyl alcohol solvent. Phenolic hydroxyl were examined and inspected by colour-developing reaction and the content of total polyphenols was determined by UV-spectrophotometry. Screening test were performed to the antifungal activities of polyphenol extraction by the tube dilution method to the selected ten pathogenic fungus such as Dothiorella gregaria, Rhizoctonia solani, Phyllosticta pirina, and the minimal inhibitory concentration were measured. The column chromatography and thin layer chromatography were applyed to separate and analyse cortex extracts of Dracaena cochinchinensi Lour.
实验部分采用以石油醚脱脂，甲醇为溶剂的回流提取法来提取植物多酚，用显色反应检识酚羟基；采用紫外分光光度法对多酚粗体物进行总多酚含量的测定；选取了聚生小穴壳菌、立枯丝核菌、梨叶点霉等10株常见农林业致病真菌，采用试管液基稀释法对多酚粗提取物进行了抗真菌活性筛选试验，并测定其最低抑菌浓度；采用柱层析法和薄层层析法对其中抑菌效果较好的龙血竭进行分离分析，根据其中3种化合物的光谱数据UV、IR、~(13C-NMR、1H-NMR和MS鉴定其结构。
The Deltamethrin residues were extracted with acetone-petroleum ether, purified by chromatography on anhy-drous sodium sulfate aluminum oxide neutral, and determined by GC-ECD.
样品用丙酮-石油醚提取，无水硫酸钠-中性氧化铝柱净化后，用带ECD的气相色谱仪进行检测。
Chloropicrin in vegetables and fruits was determined by gas chromatography with ECD after extracted with petroleum ether under ultrasonic.
采用石油醚对蔬菜、水果样品进行超声波提取，以气相色谱电子捕获检测器测定氯化苦残留。
The EtOH extract was successively fractionated with petroleum, EtOAc and n-BuOH. The resultant fractions were separated by a combination of silica gel, Sephadex LH-20 column chromatography, and preparative HPLC to afford three compounds (1—3) from the petroleum ether-soluble fraction, four compounds (4—7) from EtOAc-soluble fraction and three compounds (8—10) from n-BuOH-soluble fraction.
将新鲜致命鹅膏子实体用95%乙醇提取，乙醇提取物依次用石油醚、乙酸乙酯和正丁醇萃取分部，对石油醚萃取部分，乙酸乙酯萃取部分和正丁醇萃取部分用硅胶柱层析、反相柱层析和Sephadex LH-20凝胶柱层析、HPLC进行分离，结果从石油醚可溶部分离得到了化合物（1—3），从乙酸乙酯可溶部分离得到了化合物（4—7），从正丁醇可溶部分离得到了化合物（8—10）。
Methods:(1) To separate stilbene glycoside by using silica gel column chromatography with gradient elution consisting of ethyl acetate and petroleum ether.
硅胶柱色谱法，乙酸乙酯-石油醚梯度洗脱，自何首乌中提取分离二苯乙烯苷单体成分。
Compounds were extracted by 90% ethanol-petroleum ether and acetoacetate, then purified by silica gel column chromatography.
采用硅胶柱层析进行分离纯化，根据理化性质和波谱学数据进行结构鉴定。
Methods:The cholesterol in milk tea was directly extracted by petroleum ether, and purified by anhydrous sodium sulfate and high speed centrifugation was developed, then detected by gas chromatography-flame ionization detector.
样品中加入一定量无水硫酸钠，石油醚作为提取液，超声提取后，高速离心，利用气相色谱仪的毛细管色谱柱和氢火焰离子化检测器对上清液进行分离和检测。
It, s allelchemicals were isolated through extraction, thin-layer chromatography, and column chromatograpathy. After the white clover was infused in ethanol and evaporation, the leavings was dissolved by water. The aqueous extract was extracted by petroleum, chloroform, ethyl acetate and n-Butanol successively, and the bioassay showed that the chloroform phase has obvious inhibitory effect, the inhibitory rate was 44.32%, 88.08% on Abutilon theophrasti M., seedling length and root length, 82.08%, 92.16% on Echinochloa crusgalli L., seedling length and root length, and the Amaranthus retroflexux L., germination rate is 0, the secondly was ethyl acetate phase.
运用萃取、薄层层析、柱层析等方法对白三叶草地上部分的化感物质进行初步分离，结果表明，白三叶草经乙醇浸提旋转蒸发后用蒸馏水溶解，依次用石油醚、氯仿、乙酸乙酯、正丁醇萃取，生物测定表明氯仿组分对杂草的抑制作用最强，对苘麻芽长和根长抑制率分别为44.32%、88.08%，对稗草芽长和根长的抑制率分别为82.08%、92.16%，反枝苋的发芽率为0；其次是乙酸乙酯相。
Coenzyme Q10 is obtained through saponification, extraction with petroleum benzine, chromatography with silica gel and crystallization with ethanol.
采用醇碱皂化法，经石油醚萃取、硅胶柱层析，无水乙醇结晶、重结晶得到辅酶Q10。
Analysis of Light Components of Petroleum Hydrocarbons in Waste Water with Micro-column Adsorption Gas Chromatography
微型柱吸附-气相色谱法分析废水中石油轻组份含量
Applications of petroleum fraction analysis by capillary gas chromatography_atomic emission spectrometry are presented in this paper.
介绍了毛细管气相色谱和原子发射光谱联用仪（GC＿AED）在石油馏分分析中的几个应用实例。
A simple and rapid method has been developed for simultaneous determination of cholesterol and α tocopherol in eggs. The method involves alcoholic KOH saponification of the samples, extraction with petroleum ether, and determination by wide bore capillary gas chromatography.
采用20mol/L的KOH乙醇溶液直接皂化，石油醚萃取，大口径毛细管气相色谱法测定鸡蛋中的胆固醇和α维生素E（αVE），讨论并确定了鸡蛋样品皂化的最佳条件。
The alcoholic extract of French landaise geese gall was extracted by petroleum ether and isolated by silica column chromatography respectively, a white oily component could be obtained.
朗德鹅胆经乙醇浸提得醇提物，然后经石油醚萃取，硅胶柱层析分离得到白色油状组分，采用气相色谱-质谱联用技术进行了组成及相对含量分析。
Δ~9-Tetrahydrocannabinol was first extracted from hemp seed oil with methanol-petroleum either, and then purified by a column of activated alumine, was determined by gas chromatography.
用甲醇石油醚提取大麻籽油中的Δ9四氢大麻酚，然后通过活性氧化铝柱层析净化，再对其进行气相色谱测定。
Determination of Petroleum Monosulfonates and Petroleum Disulfonates in Crude Oil by Pseudo-multidimensional Anion-exchange Chromatography
拟多维离子交换色谱测定原油中的单双石油磺酸盐
A " U " Shape Pressurization Device Designed for the Pressurized Liquefied Petroleum Gas Injection Analysis on Gas Chromatography
一种用于液化石油气体气相色谱分析的U型增压进样装置