Different strains have different reaction to the delay of oviducts in body after animal death. After 10min delay C57BL/6 mouse oocytes have death rate of 56.5%, significantely higher than Kunming mouse (47.6%Spontaneous activation rates were respectively 13.3% and 46.0%, C57BL/6 were obviously lower than Kunming mouse.4. The oviducts were obtained after being delayed 5min 24h after the mice were injected with hCG. The oocytes were cultured in CZB. About 81.1% occurred spontaneous activation, evidently lower than parthenogenetic rate (96.4%) with SrCl_2. Spontaneous activable oocytes had high cleavage rate(93.2%) and 4-cell rate(87.3%). However, spontaneous activable oocytes had blastula development rate(18.7%) as low as parthenogenetic oocytes by SrCl_2(22.9%).
不同品系小鼠卵母细胞对输卵管在体内滞留产生的反应不同，滞留10min C57BL/6系小鼠卵母细胞死亡率为56.5%，显著高于昆明鼠(47.6%)；自发激活率分别为13.3%和46.0%，C57BL/6系小鼠显著低于昆明鼠。4.hCG后24h体内滞留5min卵母细胞在CZB中培养自发激活率为81.1%，显著低于SrCl_2孤雌激活率(96.4%)；自发激活的卵母细胞有较高的卵裂率(93.2%)和4-cell比率(87.3%)，但囊胚率(18.7%)较低，同卵龄的卵母细胞经SrCl_2孤雌激活囊胚发育率为22.9%，差异不显著。
And there were statistical differences at 0.05 level of the parthenogenetic cleavage rate and blastocyst rate between development of parthenogenetic embryos in 3, 2 and 4 h.
结论］在39℃，3h内进行孤雌胚胎的运输，能获得较高的卵裂率、囊胚率。
The effects of different temperatures (37, 38, 39) on parthenogenetic embryo cleavage and blastocyst rate and the effects of different time (2, 3, 4 h) on parthenogenetic embryo cleavage and blastocyst rate were studied.
方法］研究不同温度(37、38、39℃)对孤雌胚胎卵裂率、囊胚率的影响；不同时间(2、3、4h)对孤雌胚胎卵裂率、囊胚率的影响。
During the first 3 days of sequence culture, SOF and granulosa cell enhanced the development of porcine parthenogenetic embryos and the cleavage rate (P.05) and the number of beyond 4-cell embryos. During the following 3 days. NCSU-23 supplemented with FCS and POEC supported the embryo to morulae/blastocysts.
在序贯培养的前3d，SOF培养基和颗粒细胞对胚胎的发育有促进作用，分裂率(P.05)和突破4细胞阻滞的数目显著增加，在培养的后3d，添加胎牛血清的NCSU-23和输卵管上皮细胞能支持较多胚胎发育到桑囊胚，桑囊胚的发育率为(59.5±3.2)%(P.05)。
In this experiment were three steaming water and Mill-Q ultrapure water prepared culture medium Parthenogenetic activation of bovine embryos, results showed that the rate of oocyte maturation (PbI emission rate) and the cleavage rate and blastocyst rate of non-significant effect (P 0.05).
本试验分别用三蒸水和Mill&Q超纯水配制的培养液培养牛孤雌激活胚胎，结果表明，卵母细胞成熟率（PbI排放率）和卵裂率及囊胚率等无显著影响（P0.05）。
Our study includes four aspects. In the first aspect we study several important conditions of porcine oocytes maturation in vitro and oocytes cleavage after parthenogenetic activation and found mNCSU-23+15IU/mlPMSG+20IU/mlHCG+15% PFF+0.57mMcysteine is a good culture condition. When the Cocs are cultured in it, the maturation rate and oocytes cleavage rate are higher than those of foreign covered. Our result are (86.7±3.35)% and (86.3±4.16)% and the highest report of foreign is(85.7±4.1)%.In the second aspect we study the effect of different chemical activations on development of porcine parthennogenetic embryo and found two best activation method. The first one is that putting the maturation MII oocytes in the 20μmol/L ionomycin for 30 minutes and then putting them in the NCSU-23 condition containing 5μg/mICB and 5mM/L6-DMAP for 3.5 hours, the oocytes cleavage rate and morulae/blastocysts development rate are (76.7±7.6)% and (37.1±6.4)%.The second one is that putting the maturation MII oocytes in the 200μM/L Thimerosal for 20 minutes and then putting them in the NCSU-23 condition containing 8mM DTT for 30 minutes
本研究分为4个部分，第一部分对影响猪卵母细胞体外成熟和孤雌激活后胚胎分裂的几个重要条件进行了比较研究，确立了一种较好的培养方法：与颗粒细胞共培养，找到了一种适合猪卵母细胞体外成熟的培养基：mNCSU-23+15IU/mlPMSG+20IU/mlHCG+15%PFF+0.57mM半胱氨酸，成熟率和分裂率分别为(86.7±3.35)%和(86.3±4.16)%，国外报道的最高成熟率为(85.7±4.1)%；第二部分对猪卵母细胞孤雌激活的化学方法进行了研究，确立了化学激活猪卵母细胞的两种最佳方法：1将成熟的去卵丘颗粒细胞的MII期卵母细胞用20μmol/Lionomycin作用30min，再将卵母细胞培养于含5μg/mlCB和5mM/L 6-DMAP(6-二甲基氨基嘌呤)的NCSU-23培养液中，卵裂率和桑囊胚发育率达到(76.7±7.6)%和(37.1±6.4)%2将成熟的去卵丘颗粒细胞的MII期卵母细胞在200μM/L的Thimerosal中处理20min，再与8mM的DTT共孵育30min，卵裂率和桑/囊胚形成率为(81.0±2.8)%和(39.6±2.7)%；第三部分对孤雌激活胚胎的培养条件进行了研究，确立了一种最佳的胚胎培养条件：在SOF简单培养基中添加颗粒细胞进行前3天的培养，然后转入添加胎牛血清的NCSU—23培养基并和输卵管上皮细胞进行后期的培养，其桑椹胚和囊胚的发育率为(59.5±3.2)%；第四部分研究了IGF-I
Effects of different protein additive on sheep oocytes first cleavage after parthenogenetic activation were compared.
本文还对比了不同蛋白质添加剂对绵羊孤雌激活卵母细胞第一次卵裂的影响；
The results showed that: the maturation rate of oocytes and parthenogenetic embryo cleavage rate of the experimental groups were higher than the control group, but there was no significant difference (P0.05).
结果表明：试验组成熟率、卵母细胞孤雌后胚胎卵裂率都比对照组高，不过差异均不显著（P＞0.05）。
The experiment showed that the culture system of SOF + 10% FCS facilitates the cleavage of parthenogenetic porcine embryos. NCSU-23 supplemented with FCS and granulosa cell improved the development of porcine parthenogenetic embryos and the rate of morulae/blastocysts (P < 0.05).
试验表明：孤雌激活卵母细胞在SOF+10%FCS培养体系中分裂效果最好，添加胎牛血清的NCSU-23和颗粒细胞对胚胎发育有促进作用，培养6d后发育到桑囊胚的比率增加（P<0.05）。