The PCR product was purified by UNIQ-10 Column DNA Purification Kit to remove salts and primer-dimer. PCR product was cleaved by Msp I and HaeIII at the same time. The optimum separation condition in separating smaller than 70bp DNA fragments was applied in analyzing the mutation of codons 248 and 249 in p53 gene. The optimum separation condition was 8%LPA I with 8%mannitol, running pH at 6.5, running temperature at 15℃and electric field strength at 275V/cm. Mutation detection was completed in about 30min. Baseline separation and higher resolution (1.642) were obtained in separating 35bp and 40bp DNA fragments.
限制性内切酶MspⅠ和HaeⅢ同时切割纯化的PCR产物，然后以NGS-CE分离小于70bp DNA片段时获得最高分离度的电泳条件(筛分介质为8％LPAⅠ、添加剂甘露醇浓度为8％、pH为6.5、温度为15℃、电场强度为275V／cm)分析临床59例胃癌患者p53基因扩增产物的限制性片段长度多态性。30min内同时检测了p53基因中248位和249位两个密码子位点的突变情况，实现了35bp和40bp DNA片段的基线分离，且得到较高分离度(1.642)。
The problem of how to choose optimum charge and shooting coupling effect in high resolution seismic exploration is discussed theoretically.
引 言 在高分辨率地震勘探系统工程中，震源系统是第一个子系统，其中井深、药量、耦合条件等又是这个子系统中的重要环节。
Then, I analyze its operational principles and characteristics, and choose an optimum 1.5bit resolution per stage.
接着分析流水线电路的工作原理和特点，选择采用1.5位的最优的每级分辨率。
Optimum column pressure, plate height, resolution, tailing factor and resistance coefficient of mass trunsfer were determined.
由于薄涂色谱柱柱子短，较复杂组分的分离效果欠佳，因而限制了它的应用。
The applicability of orthogonal array designs for the optimization of separation in micellar electrokinetic capillary chromatography were investigated. The effect of four parameters were included pH, concentrations of SDS, CD and methanol, which were likely to affect the quality of the separation. Optimum separation conditions were obtained, which gave good resolution of the components of o、m、p-Nitroluene and 2, 4-Dinitrotoluene within 30min.
(1)采用正交设计法对胶束毛细管电泳中的优化分离进行了考察，研究了改性剂浓度、十二烷基硫酸钠及β-环糊精浓度、pH几个参数对分离的影响，其优化结果表明：在30min内，性质相近的邻、对、间-硝基甲苯及2，4-二硝基甲苯都可得到较好的分离，该方法与国内外类似方法相比更加简便，实验成本更低。
Even if flying outside the atmosphere, in order to ensure hit-kill the target, the common radar seeker had better not be used either. It is possible for the TBM defense interceptor to hit to kill the target or to kill the target to draw support from a strengthen kill-device with a radar seeker of high range resolution ratio or a high precision LWIR stare-image seeker, by gas thrust vector control system or gas thrust vector control/aerodynamic control system, to make use of optimum guidance law. The statically stable interceptor with gas dynamic control system in the atmosphere has the advantages that the fuel consumption of the attitude control engine is small and the error of body's angular velocity is little in the period of stabilization control. To achieve long distance ascent-phase intercept, such as the ground-launched TBM defense interceptor located in the point TBM attacking, very high demands are raised to the TBM defense system and the TBM defense interceptor, because the increase in interceptor mass is about one percent if the stand off range of the interceptor increases one kilometer, and the increase is about six percent if the intercept altitude decreases one kilometer or the reaction time of the TBM defense system increases one second for flight time of 60 to 90s, altitude of 50 to 80km and stand off range of 300 to 600km.
理论研究与数字仿真结果表明：大气层内采用普通雷达导引头的反TBM拦截器无论采用空气动力控制还是直接侧向力控制，都难以实现与目标的直接碰撞，即使在大气层外飞行，要确保与目标直接碰撞，也不宜采用普通雷达导引头，而采用高距离分辨率雷达导引头或高精度LWIR凝视成像导引头，利用直接侧向力控制或直接侧向力／空气动力复合控制方式，运用最佳制导规律的反TBM拦截器实现与目标的直接碰撞或利用杀伤增强装置杀伤目标是可能的；大气层内燃气动力控制的静稳定拦截器稳定控制时具有姿控发动机能耗少、弹体角速率误差小的优点；远距离拦截处于上升段TBM时，如采用在TBM攻击区域的地基拦截方案，对TBM防御系统及反TBM拦截器均提出了很高的要求，因为射程每增加一公里，拦截器的质量将增加百分之一左右，拦截高度每降低一公里或TBM防御系统反应时间每增加一秒，反TBM拦截器的质量将增加百分之六左右（针对射程300～600km，拦截高度50～80km，飞行时间60～90s的情况）。
Through analyzing the resolution capability of the continuing-wave PRE-IOG, optimum parameter design, modulation and demodulation schemes are presented to acieve the best sensitivity and resolution capability.
首次提出并实现了双向同时激射的AR-IOG的光纤仿真系统，证明了在坏形腔中引入适当的机制可以获得单纵模、强度相当的双路同时起振，为今后实现AR-IOG提供了实验基础。
Resolution bandwidth and sweep time automatically track the selected frequency span to ensure optimum performance, or can be manually controlled.
解决带宽和扫描时间自动追踪选定的频率跨度，以确保最佳的性能，也可以手动控制。
The spectrum is tuned to the vicinity of the center of the screen, and the reference level, resolution bandwidth, video bandwidth and sweep time are automatically set at optimum values.
频谱调整，以附近的萤幕的中央，和参考水平，决议带宽，视频带宽和扫描时间是自动设定在最佳值。
Our calcium tungstate screens use high quality phosphor processing for exceptional luminescence with an optimum combination of speed and resolution along with minimum mottle.
我们的钨酸钙增光屏使用高质量的磷，采用荧光处理，配合素和分辨率的最佳组合以及最低的斑点率。
The calculations show that the approximation for the birefringence and the rotatory property of interaction material leads to an error in the optimum incident angle for TeO2 more than 10%, a diviation in fa-λ0 relation from experiment results, and make the spectral resolution and angular aperture of the device to be degrade.
结果指出，在该波长下，对晶体双折射取近似表达式和不计入晶体的旋光特性，对光波最佳入射角θi的选取(在θi小于60°的范围内)，将引入大于10%的误差，这个误差由于涉及到是否满足动量匹配条件，因此会对器件的性能有直接的影响。
The lens'resolution capabilities; The film's resolution capabilities; Camera and/or subject motion; Aperture used and consequent depth offield as well as use of optimum aperture. Film flatness — is thepressure-plate doing its job properly?
镜头的解像能力；胶卷的解像能力；相机或被摄体的移动；所用的光圈以及其相应景深；是否使用了最佳光圈；胶卷平度－相机的压力盘（压住胶卷的那个小长方形盘）正常工作吗？
The tunable grating filtering detection system of fiber grating sensor is investigated theoretically and experimentally. The theoretical expression of the wavelength resolution is presented. The optimum theoretical wavelength resolution of the system is 0.8 pm.
对光纤光栅传感器的光栅调谐滤波检测系统进行了理论和实验研究，给出了波长测量分辨率的理论表达式，理论计算的系统最佳波长分辨率为08pm；
The Ultra drives support high resolution feedback encoders, offering optimum system performance.
Ultra驱动支持高分辨率的反馈编码器，从而提供了最佳的系统性能。
It is analyzed the factor of affecting display resolution ratio and optimum resolution ratio and discussed how to select display in this paper.
本文分析了影响显示器分辨率的因素和最佳分辨率，并讨论了如何根据分辨率选购显示器。
Collision analysis and parallel optimum resolution for dynamic contact action
强夯动接触力的碰撞分析与并行优化求解
Transcoding Based on Reducing Temporal Resolution; Process optimum proposal of reducing container yard shift rate of medium and small sized port
基于降低时间分辨率的转换编码方法降低中小码头翻箱率的流程优化方案
Meanwhile, the optimum treatment durations of the extraction solution, HR-A, and the staining solution, HR-B, in the high resolution kit were 5 min and 2 min, respectively.
同时，实验结果还表明，HR（highresolution）试剂盒中的核提取液HR-A和染色液HR-B的最佳处理时间分别是5min和2min。
The key parameters of projection display, including resolution, contrast, projection optics, optical throughput, color, light source and screen and their optimum method were described.
描述了投影显示关键参数（分辨率、对比度、投影光学、系统的光通量、彩色化、灯及屏幕）及优化方法。
Determination of the Best Grid Resolution of 1:50000 DEM Determination of Optimum Mesh Interval in Screen Printing with Poiseuille Theory
1：50000DEM最佳格网间距的确定用Poiseuille理论确定网印中的最佳网距