Variable sites, including 7 transitions, 1 transversions, and five indels (insertion/deletion) were found within 12 cultivars mtDNA sequences. The genetic distance according to Kimura model was average of 0.005, it was showed that the genetic diversity of 12 cultivars was low.
豫早1号、豫早2号、鲁红和33的序列完全一致，松黄和抗病2号的序列也相同。12个柞蚕品种之间的平均Kimura遗传距离为0.005，属于缺乏种内变异的物种。
All of the 58 restriction endonucleases were used to investigate the mtDNA diversity. Four restriction endonucleases, DraⅠ、SnaBⅠ、SspⅠ and Vsp Ⅰ, were polymorphic. Based on presence or absence of the the 215〓 SspⅠ site, all of the 13 mtDNA sequences were divided into two groups.
利用DNAClub软件对AT富集区和tRNA〓基因的DNA序列进行了模拟酶切，所有的58种内切酶中有10种内切酶在13个材料中有酶切位点，仅4种酶DraⅠ、SnaBⅠ、SspⅠ和VspⅠ表现出多态性，且主要位点差异在于SspⅠ酶切位点的有无（序列的第215位的C/T）。12个品种中有4个品种（豫早1号、豫早2号、鲁红、33）没有此酶切位点，另外8个品种有此酶切位点，野柞蚕也没有此酶切位点。
Canis lupus chanco; mtDNA; Molecular phylogenetic tree; Subspecies
中国狼；线粒体DNA ；分子系统树；亚种
Compared with the density gradient centrifugation method, this protocol was time-saving and low cost and high efficiency.
本研究建立的方法与通常采用的密度梯度离心法相比，具有耗时短、对离心机的要求不高，且所提取的mtDNA产率高、结构完整等特点。
This is the oldest complete Homo sapiens mtDNA genome generated to date.
这是最古老的智人线粒体基因组产生的日期。
The7.4 kb mtDNA deletion is one of the possible risk factors for individual susceptibility of hearing to noise exposure.
mtDNA7.4 kb缺失可能是导致听力对噪声易感性的危险因素之一。
Finding the frequency of occurrence of heterogenicity among people and making the method of examining the heterogenicity standardized by setting up the data of mitochondrial DNA will be of great sig...
通过建立mtDNA数据库，找出异质性在人群中的发生频率，并使检测异质性的方法标准化，对线粒体DNA的更有效检测具有重要意义；线粒体DNA编码区多态性位点在提高线粒体DNA系统效能中具有独到之处。
The frequency of the mtDNA^4834 bp deletion in the cochlea with polymerase chain reaction technique.
检测2组大鼠内耳丙二醛含量、听性脑干诱发电位客观反应阈、耳蜗内mtDNA4834bp缺失的变化。
With the heredity baxkground of mtDNA 4834 bp common deletion, aminoglycoside ototoxicity can not be resist compeletly by antioxidant gene therapy.
结论外源性MnSOD基因在内耳过表达能部分对抗氨基糖甙类抗生素对拟老化大鼠内耳的损伤。
Long-term treatment with Vitamin-C could reduce the degree of hearing loss of the rats and the concentrations of MDA of the cochlea and the frequency of mtDNA^4834 bp deletion in the cochlea.
结论长期使用氧自由基清除剂维生素C可以减轻大鼠内耳膜脂质过氧化程度，降低大鼠耳蜗内mtDNA4834bp缺失的发生率，从而减轻其老年性聋的程度；氧自由基学说可能是老年性聋的发病机制。
Methods The ophthalmological examinations of proband and other membere of this family and controls were conducted, and Genomic DNA was isolated from whole blood of participants. Their DNA fragments were PCR-amplified using oligodeoxynucleotides corresponding to mtDNA at positions 3108-3717 for the G3460A imitation, 11, 654-11, 865 for the G11778A imitation, and 14, 260-14, 510 for the T14484C mutation, respectively. For the detection of the three primary mutations, testing of the gene sequence were performed on the amplified PCR segments.
对LHON一个家系进行家系调查，分析其遗传特征和发病特点，并对家系成员（发病者、未发病者及对照者）进行眼科临床检查（包括视力、视野、眼底及电生理检查）和线粒体基因三个原发性突变位点的检测，即自全血提取线粒体DNA，应用聚合酶链反应技术，分别扩增mtDNA上相应片段检测G3460A、G11778A和T14484C位点突变。
It is commonly accepted that amphioxus in both Bohai Sea and Japanese waters belong to Branchiostoma belcheri tsingtauense, a subspecies of B. belcheri, according to the previous survey on the morphological characteristics.
该文测定了厦门海域和青岛海域的文昌鱼mtDNA COI和Cyt b基因的部分序列，并与日本产的文昌鱼以及产于大西洋的几种文昌鱼进行了比较。
But in anura, great variation was observed among mtDNA. the gene arrangements were compared with other anurans and the Tandem duplication followed by random gene loss model was implemented to explain for it.
但是在两栖纲无尾目动物中，线粒体基因组的基因排列顺序却存在很大变异，这种基因重排现象可以用TDRL模型来解释。
Heterodon specimens was successfully extracted by the steps of removing the formaldehyde through improved gradual dehydration first and extracting the genomic DNA by the traditional phenol-chloroform method then. After primers designed, the PCR amplification was carried out, and a 205 bp of the control region of mtDNA from all of the ten specimens was successfully amplified.
本实验通过改进的酒精梯度浸泡法去除标本中的甲醛，然后用酚-氯仿抽提法成功地提取到了铜鱼标本的基因组DNA；设计引物后进行了线粒体和微卫星的PCR扩增，扩增产物经银染检测。
Heterodon specimens as successfully extracted by the steps of removing the formaldehyde through improved gradual dehydration first and extracting the genomic DNA by the traditional phenol-chloroform method then. After primers designed, the PCR amplification was carried out, and a 205bp of the control region of mtDNA from all of the ten specimens was successfully amplified.
本实验通过改进的酒精梯度浸泡法去除标本中的甲醛，然后用酚－氯仿抽提法成功地提取到了铜鱼标本的基因组DNA；设计引物后进行了线粒体和微卫星的PCR扩增，扩增产物经银染检测。
OBJECTIVE ①To compare the sequences of the ribosomal rDNA-ITS2, 28S-D3 and mitochondrial mtDNA-COII in the different origins of Anopheles minimus for detecting the intra-and inter-specific variation of the sibling species of the Minimus Complex; ②To reconstruct phylogenetic trees of the Minimus Complex and Myzomyia Series for determining the position of An.
目的 ①比较微小按蚊核糖体rDNA-ITS2、28S-D3基因和线粒体mtDNA-COII基因的序列差异，阐明微小按蚊复合体亲缘种种间和种内变异；②根据基因变异重建微小按蚊复合体及迈蚊系成员的种系发生关系，以确立微小按蚊亲缘种变异的种系地位；③建立微小按蚊复合体亲缘种A和C的分子鉴别方法。
Total genomic DNA was extracted from four marine fishes (Verasper variegates, Cleisthenes herzensteini, Turbot Scophthalmus maximus, red seabream Pagrosomus major) using a proteinase K and phenol-chloroform procedure. Long mtDNA fragments were amplified by long and accurate PCR using primers designed based on the mtDNA sequenses of Thunnus thynnus thynnus, flounder Paralichthys olivaceus and other related fishes.
用蛋白酶k酚氯抽提法从圆斑星鲽、高眼蝶、大菱鲆和真鲷4种海水鱼的肌肉组织中提取基因组DNA，根据金枪鱼、牙鲆及相关鱼类的线粒体DNA序列保守片断设计出扩增引物，扩增从16SrRNA到ND4之间约9400bp的mtDNA长片段。
According to many methods of crops, a high-efficient technology system of BAC library construction was established. This system contains that isolation of mitochondrion, preparation of high-molecular weight mtDNA, partial digestion of megabase DNA with a restriction enzyme, selection of digested fragment and transformation with high efficiency.
本实验在借鉴了各类作物BAC文库的构建方法后，建立了一套高效的线粒体基因组BAC文库构建的技术体系，包括线粒体的分离、高质量大片段DNA的获得、大量部分酶切最佳条件的确定、片段大小的选择、高效的连接与转化体系等。
Sequence comparison and phylogenetic analysis of mtDNA 16S rRNA and COI gene fragments in four species of razor shell were made using PCR and direct sequencing. The four species are Solen grandis Dunker, Solen strictus Gould and Cultellus attenuatus Dunker, Sinonovacula constricta.
本实验对帘蛤目四种蛏类大竹蛏，长竹蛏和小刀蛏和缢蛏的线粒体16S rRNA和COI基因片段部分序列进行了比较并对其系统学进行了初步研究。
The research aimed to study the genetic diversity of mitochondrial Cytochrome b gene from Guizhou blach goats, for helping to protect Guizhou black goat breed resource and offering molecular genetics foundation for exploitation and utilization.
［目的］研究分析了贵州黑山羊mtDNA Cytb基因的遗传多样性，为贵州黑山羊遗传资源的保护、开发及利用奠定分子遗传学方面的基础。