Methods Amplify the cDNA sequence encoding truncated HCV gene, with a part of carboxyl-terminus deleted, by PCR. Synthesize the mimic epitope at E2 region of HCV and seven T or Th cell epitope genes of NS3-NS5 respectively. Bind HCV core gene with a part of carboxyl-terminus deleted to the synthetic epitope gene by PCR, then clone into eukaryotic expression vector pcDNA3.1 and transiently transfect COS7 cells.
用PCR方法扩增核心区羧基端部分缺失的基因片段；分别合成HCV E2区模拟表位和NS3~NS5 7个T或Th细胞表位基因；PCR方法将羧基端部分缺失的HCV核心区基因与合成的表位基因串联，克隆入真核表达载体pcDNA3.1，并通过脂质体瞬时转染COS7细胞。
The study team targeted the gene by giving trial participants a mimic of its natural target, ribavirin.
该研究团队为参加扎提供了一种模拟其天然靶点&利巴韦林，借此靶向研究其基因。
The RAPD Marker of Mutant Gene of Rice Disease Lesion Mimic
水稻类病变坏死突变基因的RAPD标记
The next step is to look for a compound that would mimic the effects of the gene mutation – in other words, a pill that boosts the ability to remember.
下一步是寻找可以模仿这种基因突变作用的化合物――也就是说，一种可以提高记忆力的药片。
In the previous study, fetal bovine serum and the other components such as c-kit ligand and Tpo were often used when erythroid cells were cultured in vitro. However, the fetal globin gene was reactivated in cultured erythroid cells from adult bone marrow or peripheral blood in these culture conditions. That is, these culture methods failed to mimic the erythropoiesis in physical condition and couldnt meet the needs for study of globin gene regulation and switching. In this study, serum free medium were used for induction of erythroid cells from adult bone marrow and human cord serum-containing medium were used for erythroid cells from cord blood.
鉴于在以往报告中使用的红细胞体外培养方法往往由于添加胎牛血清及诸如c-kit配体、Tpo等促生长添加剂而使得成人外周血或骨髓来源的红细胞内胎儿型γ-珠蛋白基因的表达水平升高，不能真正模拟体内成人红系分化发育的过程，无法满足研究珠蛋白表达及开关调节的需要，我们针对成人骨髓来源的红细胞尝试使用了无血清培养方法，针对人脐带血来源的红细胞则使用了含脐带血清的培养方法，并且尽量去除其他影响珠蛋白表达的添加剂，希望能够找到并建立尽可能模拟生理情况的培养条件与方法，并以此为基础，为研究珠蛋白基因表达调控及开关机制提供适当的实验材料。
Researchers have devised clever new techniques to mimic the chemical marks on tightly wound DNA-protein complexes that silence gene expression.
研究人员已经发明了精敏的新技术，来模拟化学药品，在紧密结合的沉默基因表达的DNA-蛋白复合体上做标记。
The new experiments involved creating artificial nucleosomes bearing chemically synthesized methylation marks that mimic natural gene-silencing tags.
新的试验涉及创造人工核小体携带化学合成的甲基化标记，这些标记模拟天然的基因沉默标签。