HORIZONTAL METHOD FOR THE DETECTION AND ENUMERATION OF LISTERIA MONOCYTOGENES.
单核细胞增多性李司忒氏菌的横向检查和计算法。
The lab is capable of quarantine hazardous biology such as: Phytophthora sojea, Tilletia controversa, Bursaphelenchus xylophilus, Sorghum halepense, Acanthoscelides obtectus, Bean Pod mottle virus, and practising diagnosis akabane disease, leukaemia bovum, paratuberculosis and other hazardous animal disease; detection of listeria monocytogenes, staphylococcus aureus and salmonella etc in food; examination of transgenic products such as soybean, rapeseed, corn etc and detection of bovine, sheep and goat -derived for preventing bovine spongiferm encephalopathy.
检测的项目主要有：大豆疫病、小麦矮腥黑穗病菌、松材线虫、假高梁、菜豆象、菜豆英斑驳病毒等植物危险性有害生物，赤羽病、白血病、副结核等动物危险性有害生物，单增李斯特氏菌，金黄色葡萄球菌，沙门氏菌等致病性食品微生物，大豆、油菜籽、玉米等转基因产品，以及预防疯牛病的牛羊源成分检测等。
The plant Quarantine Lab had intercepted more than 200 quarantine pests such as: Tilletia indica, Tilletia controversa, Bursaphelenchus xylophilus, sorghum halepense, Acanthoscelides obtectus, Sternochetes mangiferae, Bactrocera dorsalis, Coptoermes curvignathus for nearly 5000 batches; The Animal Quanantine Lab had intercepted about 20 cow which had some pathogens evidence of infectious disease such as: bovine infectiouse rhinotracheitis, leukaemia bovum, paratuberculosis and so on; The food microorganism lab had detected over 300 unqualified products such as Salm-Sury, Listeria monocytogenes, Mycobacterium paratuberculosis etc.
多年来，分中心各实验室切实履行严格把关的职责，植物检疫共截获大豆疫霉病菌、小麦印度腥黑穗病菌、小麦矮腥黑穗病菌、松材线虫、假高梁、菜豆象、芒果果核象甲、桔小实蝇、大家白蚁等国家禁止进境的危险性有害生物220 多种近10000 批次；动物检疫发现感染牛传染性鼻气管炎、牛白血病、副结核等传染病的疫牛20 多头；食品微生物检测共检测沙门、单增、副结核的不合格商品300 多批；分子生物学实验室为国内企业出具非转基因证书和不含有牛羊源成分证书50 余份，使相关的产品能够顺利出口到欧盟、韩国和日本等国。
In recent years, medical science progresses, the Gram-positive bacilli have a more in-depth study. Here on Gram-positive bacilli in the mononuclear cells of Listeria monocytogenes, Diphtheroid bacilli, erythema silk erysipelas bacteria of Clostridium perfringens and Clostridium difficile, Bacillus anthracis and Nocardia infections, Detection and treatment are reviewed.
近年来，医学科学不断进步，对革兰阳性杆菌有了更深入的研究，为此就革兰阳性杆菌中的单核细胞增生性李斯特菌、红斑丹毒丝菌、产气荚膜梭菌、艰难梭菌，炭疽杆菌以及诺卡氏菌的感染、检测方法及治疗作一综述。
It is caused by the germ Listeria monocytogenes, which has a special ability to survive in adverse conditions.
它是由细菌李斯特菌，其中有一个特别的能力，生存在恶劣的条件。
Objective To establish a fast and accurate method to examine the Listeria monocytogenes in foods.
目的：建立一种快速准确检验食品中单核细胞增生李斯特氏菌的方法。
Objective: To study the sensitivity of PCR method for the detection of Listeria monocytogenes in food.
目的：研究PCR检测不同食品中产单核李斯特菌的灵敏度。
From the public health point of view, Listeria monocytogenes as one of the most important food-borne pathogen, it has been paid much more attention of the people since 1990s. Many experts and scholars at home and abroad study the biological characteristics, detection technology, pathogenicity gene, virulence factors and epidemiological of Listeria monocytogenes.
从公共卫生学角度，单增李斯特菌作为一种重要的食源性传染病的致病菌，自20世纪90年代以来，更加倍受人们的关注，国内外许多专家学者针对单增李斯特菌的生物学特性、检测技术、致病基因及其毒力因子、流行病学进行了研究。
Objective The Listeria monocytogenes are widely distributed in nature and can be found on decaying vegetable and in soils, animal feces, sewage, silage, and water.
中文摘要：目的单核细胞增生性李斯特氏菌(Listeria monocytogenes，LM)广泛存在于自然界，如腐败的蔬菜、土壤、动物粪便、污水、储存饲料。
ResuLts indicate that ten species of pathogenic bacteria(Staphylococcus aureus, Clostridium botuLinum, Clostridium perfringens, Shigella sonnei, Vibrio cholerae, Proteus vuLgaris, Vibrio mimicus, Listeria monocytogenes, Yersinia enterocolitica, Bacillus cereus) show high sensitivity and specificity for the oligonucleotide array; Vibrio parahaemolyticus showed low sensitivity for the oligonucleotide array; Vibrio fluvialis gave weak cross-reaction with Vibrio parahaemolyticus; Streptococcus hemolytic-βgave weak cross-reaction with Listeria monocytogenes and Bacillus cereus; but the reaction did not affect their detection.
结果表明10株食源性致病菌(金黄色葡萄球菌、肉毒梭状芽孢杆菌、产气荚膜梭菌、宋内氏志贺氏菌、霍乱弧菌、普通变形杆菌、拟态弧菌、单核增生李斯特菌、小肠结肠炎耶尔森氏菌、腊样芽孢杆菌)的检测具有高敏感性和特异性；副溶血性弧菌的敏感性相对较低；河流弧菌同副溶血性弧菌存在很弱的错杂交，乙型溶血性链球菌的检测同单核增生李斯特菌和腊样芽孢杆菌存在很弱的错杂交，但是均不影响检测结果。
Diarrheogenic Escherichia coli, Listeria monocytogenes, Salmonella and Vibrio parahaemolyticus were four common food-borne bacterial pathogens in innocuous poultry and seafood.
肠出血性大肠杆菌、单核细胞增生性李斯特菌、沙门菌和副溶血性弧菌是无公害畜禽肉和水产品中常见的4种些致病菌的致病性与其毒力因子密切相关。
Listeria monocytogenes is an anaerobic, gram-positive bacilus that primarily affects immunocompromised hosts and is uncommon in the general population.
李斯特菌是一种厌氧性、革兰氏阳性菌，主要累及免疫抑制病人，在普通人群中少见。
The total of forty-seven strains of lactic acid bacteria were not screened for antagonistic activities to Staphylococcus aureus and Escherichia coli. The bodies of only ten Lactobacilli were shown to inhibit the growth of indicator organism-Listeria monocytogenes or Bacillus subtilis among twenty-six Lactobacilli. Nine Lactocilli also exhibited inhibitory activity against indicator organism. Among them there are seven strains which not only the supernatants but also the bodies exhibited inhibitory activity against indicator organism- Listeria monocytogenes or Bacillus subtilis and six of seven Lactocilli were Enterococcus faecalis. Two of the seven Lactocilli, 3-3-2 and 4-3-2, which have higher inhibitory activity were chosen for further study.
结果表明，47株乳酸菌的发酵上清液和菌悬液均不能抑制Staphylococcus aureus和Escherichia coli；26株乳杆菌中，有10株乳杆菌仅有菌悬液有抑制指示菌活性，其中6株乳杆菌菌悬液有抑制Listeria monocytogenes和Bacillus subtills的活性，4株乳杆菌菌悬液仅有抑制Listeria monocytogenes的作用。21株乳酸球菌中，9株菌对指示菌有抑菌活性，其中2株菌仅有菌悬液对指示菌有抑制作用，而7株菌不仅菌悬液，发酵上清液也具有抑制指示菌活性，而且Enterococcus faecalis占多数为6株。
WHO has listed the Listeria monocytogenes as one of the four big food pathogenic bacteria of the 1990s (enteropathogenic E. coli, Clostridium botulinum, Aeromonas hydrophila and LM), simultaneously it has been listed as one of the twelve kinds of pathogenic microorganisms which is the 21st century to have to the Chinese health the significant influence, the disease lethality caused by it has reached as high as 30~40%.
WHO将单核细胞增生性李斯特杆菌(Listeria monocytogenes，LM)列为20世纪90年代四大食品致病菌(即致病性大肠杆菌，肉毒梭菌，亲水气单胞菌和LM)之一，同时被列为21世纪对中国人卫生健康具有重大影响的12种病原微生物之一，由其引起的疾病致死率高达30~40%。
Methods According to national standards of GB 4789 and international food-borne pathogens test method, the Salmonella, the Vibrio phrahaemolyticus, the Listeria monocytogenes, EHEC (O157:H7), the Staphylococcus aureus and the Campylobacter jejuni were isolated from 8 kind of foods, and identified by biochemistry and serotype.
按国标方法和2005年全国食源性致病菌规定方法，对8类食品进行沙门菌、副溶血弧菌、单增李斯特菌、肠出血性大肠埃希菌、金黄色葡萄球菌、空肠弯曲菌分离、生化及血清型鉴定。
About 97% of Listeria monocytogenes isolates from salmon imported colud cause the Listeriosis in human.
首次对分离自挪威三文鱼的单核细胞增生李斯特菌进行血清分型，所分离的菌株中97%是引起人类李斯特菌病的菌株。
Nested Primers based on special insertion sequence IS6110 of M. tuberculosis were designed, which were used to amplify M. tuberculosis genome DNA. This study developed the nested-PCR diagnosis method for detecting M. tuberculosis, which was verified with the specificity and sensitivity. 244 bp fragment was obtained by amplifing the M. tuberculosis with the nested-PCR diagnosis method, Staphycococcus aureus, streptococcus, E. coli, Salmonella, Corynebacterium, Pseudomonas aeruginosa, Listeria monocytogenes, Brucella, et al were detected. The sensitivity of the nested-PCR diagnosis method is 4 copies/reaction by detecting the ladder concentration of simulation positive sputum samples.
以结核分枝杆菌特有的插入序列IS6110设计巢式PCR引物，对痰样本结核分枝杆菌的基因组DNA进行扩增，建立了检测结核病病原的巢式PCR诊断方法，试验验证有较好的特异性和敏感性，采用此方法扩增结核分枝杆菌可以获得244 bp的目的片段，对常见病原菌金黄色葡萄球菌、链球菌、沙门氏菌、大肠杆菌、李氏杆菌、布鲁氏菌等以及无结核病史的痰样本检测阴性，通过扩增不同浓度梯度稀释的模拟阳性痰样本确定了其敏感性为4个拷贝／反应。
in order to establish a rapid, sensitive and specific detection method for identification of four kinds of food-borne pathogenic bacteria including salmonellla spp, listeria monocytogenes, staphylococcus aureus and bacillus cereus by using multiplex pcr, according to invasion protein a geneof salmonellla spp, transcriptional regulatory protein gene of listeria monocytogenes, autolysin gene ofstaphylococcus aureus and gyrase b subunit gene of bacillus cereus, four pairs of specific primers were designed for multiplex pcr amplification.
小 摘要：为建立一种利用多重pcr技术检测和鉴定沙门氏菌、单核细胞增生李斯特菌、金黄色葡萄球菌和蜡样芽孢杆菌的方法，根据沙门氏菌侵袭力蛋白a基因、单核细胞增生李斯特菌蛋白转录调控基因、金黄色葡萄球菌自溶素基因、蜡样芽孢杆菌的促旋酶b亚单位基因设计引物，进行多重pcr扩增，并对多重pcr反应体系进行优化。
On today`s Vancouver Sun: 12 deaths in Canada (11 in Ontario and 1 in British Columbia) have now been linked to bacterium Listeria monocytogenes.
根据今天星岛日报报导：加拿大已经有12宗死亡证实与李斯特氏杆菌感染有关(11宗在安大略省和1宗在卑斯省)。
We developed single base extension-tags microarray to detect eight common food-borne pathogens, including Staphylococcus aureus, Vibrio parahaemolyticus, Listeria monocytogenes, Salmonella, Enterobacter sakazaki, Shigella, Escherichia coli O157:H7 and Campylobacter jejuni.
针对8种常见的食源性致病菌(金黄色葡萄球菌、副溶血弧菌、单核细胞增生李斯特菌、沙门氏菌、阪崎肠杆菌、志贺氏菌、肠出血性大肠杆菌O157：H7和空肠弯曲杆菌)，建立了基于单碱基延伸标签反应原理的基因芯片检测方法。