To evaluate the specificity of the PCR, genomic DNA of Theileria annulata, Babesia bovis, Toxoplasma gondii, Leishmania donovani and standard strain of N. caninum were used as a template in the PCR. For determining the detection limit of amplification procedure, PCR was run on a dilution series of genomic DNA from N. caninum（1.5625-200 ng/ml）. Brain tissue samples of 32 aborted fetuses were detected by PCR-based assay, and 23 blood samples from mothers were tested by ELISA. Results The amplified DNA fragment （350 bp）had a high identity of 98% with the Nc-5 gene sequence of N.
以环形泰勒虫、牛巴贝斯虫、刚地弓形虫、杜氏利什曼原虫以及犬新孢子虫标准株DNA为模板进行扩增以验证PCR的特异性，采用紫外分光光度计测定犬新孢子虫标准株DNA浓度和纯度，取高纯度的DNA样品用灭菌水稀释，分别取不同量的DNA进行PCR扩增，确定PCR方法的敏感性；利用该方法对32份奶牛流产的胎牛脑组织样品进行检测，同时，对其中23份流产的母牛血样进行ELISA血清学检测，以评价犬新孢子虫PCR方法的检测效果。
Application of monoclonal antibodies against Leishmania donovani & ⅱ. detection of circulating antigen in sera of visceral leishmaniasis before and after treatment
抗杜氏和什曼原虫单克隆抗体的应用&Ⅱ.内脏利什曼病治疗前后循环抗原的检测
Cloning and analyzing sequence of LACK protective antigen gene from leishmania donovani isolated from hill foci of China
我国山丘疫区杜氏利什曼原虫LACK保护性抗原基因克隆和序列分析
PCR amplification of Leishmania donovani kDNA for diagnosis of canine visceral leishmaniasis
PCR扩增技术用于犬内脏利什曼病病原体kDNA的检测
Cloning of Leishmania donovani kinetoplast DNA minicircles
杜氏利什曼原虫动基体DNA微环的克隆
Researchers evaluated the efficacy of artemisinin against the Leishmania donovani parasite.
科学家评估了青蒿素治疗杜氏利什曼原虫（Leishmaniadonovani）的效果。
The researchers identified proteins produced only by Leishmania donovani, the parasite that causes the disease.
一组科学家发现了仅由导致黑热病的杜氏利什曼原虫（Leishmaniadonovani）产生的蛋白质。
Ultrastructural localization of Leishmania donovani antigen by immunogold labeling technique
胶体金标记杜氏利什曼原虫抗原超微结构定位研究
Cloning and Expression of Extracellular Region Gene Located in N-terminus of Leishmania Donovani
杜氏利什曼原虫无鞭毛体蛋白N端胞外区基因（ast1）的克隆及表达
Cloning and expression of LACK gene of Leishmania donovani
杜氏利什曼原虫LACK抗原基因的克隆和表达
In this study, the early diagnosis of toxoplasmosis and leishmaniasis model was established by analysising the serum peptide fingerprints of normal mice and infected with Toxoplasma gondii and Leishmania donovani mice. Some related polypeptide of toxoplasmosis and leishmaniasis was found.
本实验通过采集感染弓形虫和杜氏利什曼原虫小鼠的血清多肽指纹图谱与正常小鼠进行分析比较，分别建立了弓形虫病和黑热病的早期诊断模型，筛选出弓形虫病和黑热病感染的相关多肽标志物。
Aim To clone protein phosphatase 2C (PP2C) gene from Leishmania donovani 1S parasite.
目的克隆杜氏利什曼原虫（Leishmaniadonovani，Ld）1S株蛋白磷酸酶2C（PP2C）的编码基因，为应用这种编码T细胞抗原的基因进行基因疫苗研究奠定基础。
Studies on protective monoclonal antibodies to Leishmania donovani
抗杜氏利什曼原虫保护性单克隆抗体研究
Aim: To investigate the effect of the male hormone, testosterone (Te), on Leishmania donovani infection rates and infection levels of macrophages from murine macrophage cell line (Ana 1).
目的：研究雄性激素对鼠巨噬细胞株（ana1）细胞杜氏利什曼原虫感染率及感染水平的影响。
Studies on monoclonal antibodies against Leishmania donovani Xinjiang strain experimental infection of P. alexandri and P. mongolensis with Leishmania donovani
抗杜氏利什曼原虫新疆株单克隆抗体的研究亚历山大白蛉和蒙古白蛉人工感染杜氏利什曼原虫的实验
Polymerase chain reaction (PCR) was employed to amplify RACK DNA using genomic DNA from Leishmania donovani 1S as the template.
以杜氏利什曼原虫的基因组DNA为模板，利用多聚酶链反应（PCR）技术，扩增获得了杜氏利什曼原虫RACK的全长编码基因。
Expression of cloning of Leishmania donovani genes encoding 23 kDa antigen
杜氏利什曼原虫23kDa抗原编码基因克隆的表达
A Survey of Dog Infected with Leishmania Donovani in Wen-chuan County of Sichuan Province by McAb-AST and Bone-Marrow Smear Methods
用McAb-AST及骨髓涂片法对四川省汶川县犬感染利什曼原虫的调查研究
CONCLUSION: The inhibitory effect of Mc Abs M1/70 and M18/2 on the invasion of Leishmania donovani to macrophages was achieved by blocking separately the specific binding sites of the macrophage surface molecules, Mac - 1 receptor.
结论：M1/70和M18/2可以通过与巨噬细胞表面Mac-1的结合，分别干扰巨噬细胞表面分子上与利什曼原虫相结合的不同的连接位点，抑制利什曼原虫对巨噬细胞的入侵。
Testosterone Induces Apoptosis and Enhances Leishmania Donovani Infection of Macrophages
睾酮诱导骨髓巨噬细胞凋亡并增加杜氏利什曼原虫对巨噬细胞的侵袭力