The regulatory elements of the tetracycline resistance operon of Eschenchia coli, Tet repressor and Tet operator, have served as the basis for the establishment of heterologous repression-based inducible system in many eukaryotes including yeast, plants, insects, mammals and parasitic protozoa, such as Trypanosomes, Entamoeba, Giardia, Toxoplasma, Leishmania and Trichomonas.
基于四环素抑制子(Tetracycline Repressor，TetR)和四环素操纵子(Tet Operator，TetO)相互作用的四环素可诱导转基因表达系统，已经广泛的应用于很多真核生物，包括酵母、植物、昆虫、哺乳类细胞和诸如锥虫、阿米巴、贾第虫、弓形虫、利什曼和毛滴虫的寄生原虫。
To evaluate the specificity of the PCR, genomic DNA of Theileria annulata, Babesia bovis, Toxoplasma gondii, Leishmania donovani and standard strain of N. caninum were used as a template in the PCR. For determining the detection limit of amplification procedure, PCR was run on a dilution series of genomic DNA from N. caninum（1.5625-200 ng/ml）. Brain tissue samples of 32 aborted fetuses were detected by PCR-based assay, and 23 blood samples from mothers were tested by ELISA. Results The amplified DNA fragment （350 bp）had a high identity of 98% with the Nc-5 gene sequence of N.
以环形泰勒虫、牛巴贝斯虫、刚地弓形虫、杜氏利什曼原虫以及犬新孢子虫标准株DNA为模板进行扩增以验证PCR的特异性，采用紫外分光光度计测定犬新孢子虫标准株DNA浓度和纯度，取高纯度的DNA样品用灭菌水稀释，分别取不同量的DNA进行PCR扩增，确定PCR方法的敏感性；利用该方法对32份奶牛流产的胎牛脑组织样品进行检测，同时，对其中23份流产的母牛血样进行ELISA血清学检测，以评价犬新孢子虫PCR方法的检测效果。
These results prove the practical value of this repetitive DNA fragment on the identification of the Leishmania isolates in China.
该DNA重复序列在我国利什曼原虫的鉴定上有较好的实用价值。
Objective To establish PCR method for the detection of the asymptomatic infection of Leishmania infantum.
摘要目的建立适合检测我国婴儿利什曼原虫无症状感染的PCR方法。
Objective To detect the expression level of stage-specific genes in Leishmania promastigotes and amastigotes.
摘要目的证明亚马孙利什曼原虫前鞭毛体和无鞭毛体的基因表达水平。
Therefore, iron deficiency anemia and significant reduction of blood components can be used as indicators on the severity of Leishmania infection.
统计结果表明，内脏利什曼病患者多表现为较严重的感染和缺铁性贫血以及血细胞参数显著降低，这可作为反映内脏利什曼病严重程度的重要指标。
The DNA in the samples will be tested for tuberculosis, leprosy, leishmania and malaria. But the focus is on tuberculosis, which is the biggest killer today.
其中的DNA样本将进行结核病、麻风病、利什曼原虫病和疟疾的检测，但是科学家关注的焦点仍是人类最大的杀手：结核病。
Genomic analysis of parasitic human pathogens, particularly Plasmodium falciparum, and Leishmania major.
人类寄生性病原体的基因分析，特别是疟原虫与利什曼原虫。
Application of molecular methods in identification and phylogeny of Leishmania
分子方法在利什曼原虫虫株鉴定和系统发生研究中的应用
Leishman - donovan bodies Genomic analysis of parasitic human pathogens, particularly Plasmodium falciparum, and Leishmania major.
人类寄生性病原体的基因体分析，特别是疟原虫与利什曼原虫。
Application of monoclonal antibodies against Leishmania donovani & ⅱ. detection of circulating antigen in sera of visceral leishmaniasis before and after treatment
抗杜氏和什曼原虫单克隆抗体的应用&Ⅱ.内脏利什曼病治疗前后循环抗原的检测
An new and simple method to determine the antimony (Sb) in cells using Hydride Generation-Atomic Fluorescence Spectrometry (HG-AFS) was established for studying the mechanism of metal resistance in the protozoan parasite Leishmania.
为了配合研究利什曼原虫（Leishmania）的金属抗药性，本文建立了一种氢化物发生-原子荧光法测定细胞内痕量锑的方法。
An analytical method for determination of antimony (Sb) in parasite cells using inductively coupled plasma mass spectrometry (ICP-MS) was established in order to study the mechanism of metal resistance in the protozoan parasite Leishmania.
为了研究利什曼原虫（Leishmania）对于含五价锑药物的抗药性机理而建立了一种测定细胞内痕量金属锑的方法。
Cloning and analyzing sequence of LACK protective antigen gene from leishmania donovani isolated from hill foci of China
我国山丘疫区杜氏利什曼原虫LACK保护性抗原基因克隆和序列分析
The new vaccine is based on introducing specific genes and proteins from the protozoan Leishmania infantum into the patient.
这种新疫苗的基础是引入了由利什曼幼虫得到的特异性基因和蛋白到病人体内。
Evaluation of a repetitive DNA sequence of Leishmania on the identification of Leishmania isolates in China
利什曼原虫&DNA重复序列在虫种鉴定上的价值分析
Cloning and sequence analysis of a gene encoding the receptor of activated protein C kinase (rack) from Leishmania braziliensis parasites
巴西利什曼原虫激活蛋白激酶C受体的基因克隆化与序列分析
PCR amplification of Leishmania donovani kDNA for diagnosis of canine visceral leishmaniasis
PCR扩增技术用于犬内脏利什曼病病原体kDNA的检测
The effect of immune serum against Leishmania Promastigotes on the growth of homologous and heterologous counterparts
免疫血清对同种和异种利什曼原虫的影响
Studies on Leishmania in Big Gerbils and Lizards and Its Sandfly Vectors in Ejin Banner, Inner Mongolia Autonomous Region
内蒙古额济纳旗大沙鼠和蜥蜴体内的利什曼原虫及其媒介的调查研究