The differentiation of leaf explants was inhabited on differentiation medium containing 2mg/L Hyg; all shoots that were transferred to rooting medium containing 0-2mg/L Hyg were inhibited completely to root. The effect of cefotaxime and Carbenicillin in different concentration was evaluated on inhibiting Agrobacterium LBA4404 growth, the leaf differentiation and root. It showed that Agrobacterium LBA4404 cultured on solid differentiation medium containing 600mg/L cefotaxime or 400 mg-L~'Carbenicillin was killed; in the concentration the effect of cefotaxime to leaf explants was observed than higher that of Carbenicillin and the effect of cefotaxime to shoot rooting was observed than less that of Carbenicillin.
比较了头孢唑啉钠和羧卞青霉素抑菌效果及对叶片分化和苗木生根的影响，结果表明头孢唑啉钠和羧卞青霉素浓度分别为600mg·L~(-1)和400mg·L~(-1)时，可完全抑制农杆菌LBA4404的生长：在此浓度下，头孢唑啉钠对叶片的分化影响较羧卞青霉素大，而对苗木的生根影响较羧卞青霉素小。
The inoculated leaf disks by LBA4404 carrying pArt27I〓K〓 formed light green calli at frequency of 18.9% on selection medium containing 50 mg/L Kanamycin after 10 weeks culture.
草莓叶盘在分化培养基上预培养3天，侵菌20分钟，共培养2天，用Kan 50mg／L筛选抗性愈伤和抗性芽，8周后抗性愈伤的诱导率为18.9％，12周后抗性芽的分化率为0.78%。
We routinely use the Agrobacterium strains (ABI, EHA105, LBA 4404), but believe that other Agrobacterium strains should work as well.
我们通常使用 Agrobacterium 紧张(ABI、EHA105，LBA 4404)，但是相信其他的 Agrobacterium 劳累应该也工作。
Cotyledon and hypocotyl segments of tomato were co-cultivated with Agrobacterium tumefaciens LBA 4404 harboring the binary plasmid pBI 121 (conferring β-glucuronidase activity and resistance to kanamycin) and cultured on the medium supplemented with 100 mg/l kanamycin to form shoots and select putatively transformants.
番茄之子叶或下胚轴与含质体pBI121的农杆菌LBA 4404共培养后，於含有100mg/l kanamycin的培养基上进行筛选及培养，可获得拟转殖枝条。部分具有kanamycin抗性的技条，可表现GUS活性。
A plant expression vector pArt69I3K3 carrying chicken Marek's disease virus B antigen gene was constructed by linking the gene to a constitutive promoter (CaM V 35S) and terminator (Ocs, 3'). Tobacco plants were transformed by using Agrobacterium tumefaciens strain LBA 4404 harboring pArt69I3K3. Marker genes NPT II and GUS were expressed in the putative transformants.
为探索利用转基因植物生产鸡马立克氏病疫苗的新途径，将马立克氏病病毒（Marek's disease virus，MDV） B抗原基因的两个片段（I3和K3）整合连接到相应的双元载体上，构建成带有NptⅡ和Gus基因的MDV B抗原基因的植物表达载体pArt69I3K3。
A plant expression carrier containing HBsAg (hepatitis B virus surface antigen) gene was constructed in a plasmid, the plasmid was mobilized into a rhizogenes Strain LBA 1314 by freeze-thaw method, the HBsAg gene was planted into tobacco by leaf disc via Agmbacterium tumefaciens LBA 4404 as a vehicle and the tobacco hairy roots were received.
构建了乙肝病毒表面抗原基因植物表达载体，通过冻融法将HBsAg基因转入到发根农杆菌LBA1314中，采用叶盘法将HBsAg基因导入到烟草中，获得了转基因烟草发根。
Tobacco suspension cells were transformed with Agrobacterium tumefaciens strain LBA 4404 contraining a disarmed virulence helper plasmid pAL4404 and a binary vector pBI 121 carrying chimeric genes npt Ⅱ and gus.
用A.tumefaciens菌株LBA 4404介导转化了烟草悬浮细胞，经过近一年的继代培养仍稳定表现卡那霉素抗性和GUS活性，其再生植株亦表现Km〓并检测到GUS活性。
They were then transformed to potatoes by Agrobacterium tumefaciens LBA 4404. The vector pYH144 was transferred into two potato varieties (Kexin No.1 and No.2), and the vector pYHEt was transferred into three potato varieties (Desiree, Kexin No.2 and No.4). Transgenic potato tube seedlings labelled with Hygromycin B resistance were obtained by tissue culture.
通过农杆菌LBA4404介导转化马铃薯，其中用pYH144载体转化2个品种(克新1号，2号)，用pYHEt载体转化3个品种(Desiree，克新2号，4号)，通过组织培养分别获得潮霉素标记的转基因马铃薯试管苗。
The results showed that the inclusion of acetosyringone (100μmol/L) and ascorbatic acid (50mg/L) in both infection medium and co-cultivation medium, LBA4404 concentration of fermenting liquor of OD6000.6 and infection time of 20 min were optimal transformation system.
结果表明：在感染液和共培培养基中分别都加入100μmol/L乙酰丁香酮和50mg/L抗坏血酸，农杆菌LBA4404的菌液OD600为0.6、侵染时间20min为农杆菌转化的最适条件。
Methods Transform Agrobacterium tumerfaciens strain LBA4404 with previously constructed recombinant plasmid pBI 121/, then integrate human insulin gene to ginseng callus cells by co-culture of the cells with the transformed LBA4404 strain. The expressed product was identified by Western blot and subjected to blood sugar decrease test in mice.
将已构建成的植物表达载体pBI 121/转入农杆菌田LBA4404中，通过农杆菌与人参愈伤组织细胞的共培养，将人胰岛素基因整合到人参愈伤组织细胞中，对其表达产物进行检测，并用小鼠进行降糖试验。
Culture tender leaves in culture medium of MS+2, 4-D1.5mg/L+30g/L suger+0.7% agar pH5.8 for 20 days in darkness at 25℃, and then subculture to induced Ⅱ-type calli. Use forceps cutting the tissue to nubble with 2mm2, and put the tissue into Agrodacterium tumefaciens LBA4404 liquid supplemented with AS 100mg/L, then, co-culture 3 days, resume 7 days in MS+2, 4-D1.5mg/L+30g/L suger+500mg/L cef, take to MS+2, 4-D1.5mg/L+30g/L suger+100mg/L cef+10.0mg/L kanamycin culture 20 days in darkness. After that to make it polarize in MS+30g/L suger+100mg/L cef+10.0mg/L km. The percentage of km resistant callus was reached max after infection for 45 min, the average is 29.66%. Simultaneity, tender leave callus are infected 5 min by A. tumefaciens liquid in different negative pressure, and kept on 15 min in Agrodacterium tumefaciens liquid without negative pressure. Then screen out resistant callus and obtain transgenic plants. When the negative pressure is -0.05MP the percentage of km resistant callus was reached max, the average rate is 37.5%.
将心叶接种于MS+2.4-D1.5mg/L+30g/L 蔗糖，琼脂0.7%，pH5.8 培养基中25℃暗培养20d 后继代一次，诱导产生Ⅱ型胚性愈伤组织，用镊子将其夹碎成2mm2大小的小块，置入添加100mg/L AS 的根癌农杆菌LBA4404 菌液中，侵染时间为45min，共培养3 天后，转入MS+2.4-D1.5mg/L+30g/L 蔗糖+500mg/L Cef 培养基中恢复7天，再转入MS+2.4-D1.5mg/L+30g/L 蔗糖+100mg/L Cef+10.0mg/L Km 中，遮光培养20d后，置入其MS+30g/L 蔗糖+100mg/L Cef+10.0mg/L Km 分化，卡那霉素抗性愈伤组织获得率在侵染45min 时达到最大值平均为29.66%；同时以甘蔗心叶愈伤组织材料，通过循环水式真空泵，产生负压，设定不同的负压值，在农杆菌菌液中感染5min 后，在负压条件下继续侵染15min 后，筛选出抗性愈伤组织并获得转化植株，其中在负压为-0.05 时转化率达到最大值，其卡那霉素抗性愈伤组织获得率平均为37.5%。
After purified with chromatography column, the products of RT-PCR was cloned into T-vector and sub-cloned into Nco Ⅰ/BstE Ⅱ sites of the recipient plasmid pCAMBIA1301 which was digested with the restriction endonucleases Nco Ⅰ/BstE Ⅱ, and therefore a plant expression vector named pCA, was constructed.
采用RT-PCR法从人胎盘组织克隆Arresten基因cDNA，克隆产物经酶切后定向插入到植物双元表达载体pCAMBIA1301的Nco Ⅰ和BstEⅡ位点之间，构建成含有目的基因的植物表达载体pCA，经PCR、限制性内切酶检测及序列测定正确后，转化根癌农杆菌LBA4404，获得携带目的基因的重组农杆菌。
GLDH fragment was ligated with YYT interval region at forward and reverse orientations through three sub-cloning via mid-clone vector. The fusion gene was inserted into plant vector to construct the recombinant plasmid pRNAi-GLDH, which was then transformed into Agrobacterium LBA4404. This will provide an effective tool for the further study of GLDH gene function.
构建中间克隆载体，经过3次亚克隆将测序正确的GLDH基因片段分别以正向和反向两种形式插入到间隔基因YYT的两端，经鉴定已插入到植物表达载体中，成功地构建了干扰GLDH基因表达的RNAi植物双元表达载体pRNAi-GLDH，并将表达载体转化到农杆菌菌株LBA4404中，为深入研究GLDH基因功能提供有效的工具。
In this study, three kinds of microorganisms, E. coli JM109, Agrobacterium LBA4404 and Bombyx mori nucleopolyhedrovirus, were used to infect silkworms and study the inductive expression of enbocin gene in silkworm hemocyte and fat body.
研究了家蚕经大肠杆菌(JM109)、农杆菌(LBA4404)和家蚕核型多角体病毒3种外源微生物诱导后，抗菌肽enbocin基因在蚕体血液和脂肪体中的表达。3种外源微生物诱导前、后，抗菌肽enbocin基因在蚕体血液中的表达均较弱。
The interface technology of the hard disk includes ST-506 interface, ESDI interface, IDE and EIDE interface, 33/66 DMA, SCSI interface, etc., this text has also told 3 kinds of hard disk work patterns of the hard disk: NORMAL, LBA and LARGE mode
硬盘的接口技术包括ST－506接口，ESDI接口，IDE与EIDE接口，DMA 33/66，SCSI接口等，本文还讲述了硬盘的3种硬盘工作模式：NORMAL、LBA和LARGE模式
The PPTL formula is then transformed to an Labelled Büchi Automata; further, the system model was also represented by LBA; finally, the model checking PPTL formula is done by checking whether the product of the two LBAs is empty.
通过将描述系统性质的公式转换成Büchi，进而与刻画系统模型的Büchi自动机求积判空来完成模型检查，即检查系统是否满足期望的性质。
Hairy root cultures of Astragalus membranaceus were established after roots were induced following infection of aseptic leaves with Agrobacterium rhizogenes strain LBA 9402 and factors affecting their growth were investigated.
用发根农杆菌LBA 9402菌株诱导膜荚黄芪叶外植体建立了毛状根培养系统，比较了不同外界因子对毛状根生长的影响，确定了最适培养条件，并在此基础上用3L、5L、10L体积的容器进行大规模培养实验，培养3周后毛状根生长量可达9.7g／l、10.9g／l、9.8g／l，增长倍数为28-35倍。
Hairy roots were induced from tobacco leaves with Agrobacterium rhizogenes LBA1334 harboring agropin type PRil855 and binary vector PIG121HM carrying a kanamycin resistant gene nptll and GUS gene and the good hairy root clones were selected. The root with many root hairs and branches grew vigorously toward all the direction on MS medium without plant hormone and on kanamyain containing medium (km:30mg/L). The structure of the hairy root tip is different from that of ordinary root. Witch was not typical root cap maybe the main cause of losing the geotropism of hairy root. Plantlet regenerated from hairy root when cultured on Ms medium without plant hormone. The nicotine content of the hairy root was little higher than that in the natural roots.
本研究通过发根农杆菌工程菌LBA1334(含有野生型pRi1855质粒和pIG121HM双元载体质粒，编码NPTⅡ基因和GUS基因)转化烟草沙姆逊，获得了具有卡那霉素抗性烟草发状根，并筛选出良好的单克隆株系，该发状根具有典型的发状根特性(多根毛、多分支、在无激素培养基上快速生长)，通过根尖压片分析，烟草发状根不具备典型的根冠；发状根中烟碱含量1.61％稍高于与天然栽培品种母体根的含量(1.5％左右)，发状根向培养液中释放烟碱，释放量最多时占总烟碱量的81.1％；在无激素的培养基上获得了再生植株。
Oxysporum Schl. f. sp. lycopersici germlings was detected in the extracts of transformed bacteria. Furthermore, the I- Chi and I- Glu acted syntonically.
第三，将这两种酶的cDNA构建在植物表达载体上并通过三亲交配转入农杆菌LBA4404中。
To Agrobacterium LBA4404, the effects of antibacterial Cefotaxime was best, Carbenicillin was second.
对于农杆菌LBA4404，头孢噻肟钠的抑菌效果最好，其次是羧苄青霉素。