Methods The effects of increased ICP on flow velo city, diameter of the c erebral bridging veins and pulsatility index were measured by using colo u r Doppler ultrasound in 7 Damsh landrace pigs. ICP was increased stepwise from ba seline (about 1.3kPa) to 2.7kPa and 4.0kPa by infusion of mock CSF into the cisterna magna.
选择7头丹麦白约克猪，通过向枕大池灌注人工脑脊液，逐步增加颅内压，从基础值(大约1.3kPa)到2.7kPa和4.0kPa，用彩色超声多谱勒测量正常和过度换气状态下，颅内压增高对于脑桥静脉的血流速度、直径和脉冲指数的影响。
Results of endurance on temperature indicated that both wild and domestic pigs have different reaction of none adaptation below-13℃ or above 27℃, but wild pig has stronger endurance to high and low temperature than Bamei. It shows that the range of temperature from -13℃ to 27℃ is relatively fitting the growth and activities of wild pigs. 8 Analyzing synthetically the effects of light intensity on activity frequency and intake of wild pig, Bamei and Landrace, the range of light intensity adapted to wild pig is smaller than the domestic, but the variance of light intensity has more effects on wild pig within a fitting range of light intensity.
8综合分析光照强度对野猪、八眉猪和长白猪活动频度及采食量的影响，野猪适宜的光强度范围比家猪小，但在适宜的光强度范围内，光强度变化对野猪所起的正效应较大，说明野猪对光照强度的灵敏度比家猪高，在201x～901x的光照强度区间内相对喜欢光线较弱的环境，因此，在对野猪驯养时要尽可能满足野猪的这一习性，将光强度控制在501x以内。
Genotype distribution of three mutation site tested by x~2 test showed that there was significant difference or very significant difference between Landrace and the other six breeds. There was no difference between Liaoning black, Jihebai B and Danyu black, there was significant difference or very significant difference between Liaoning black and the other four breeds.
三个变异位点基因型分布经独立性x~2检验表明长白猪与其它6个猪种之间存在显著或极显著的差异；辽宁黑猪、冀合白B、丹育黑猪三者差异不显著，辽宁黑猪与其它猪种存在显著或极显著的差异。
Three hundred and twenty-four crossbred (Landrace×Yorkshine) barrows with average body weight of 27 kg were randomly assigned to 6 dietary groups: group Ⅰ(chlorotetracycline group, added 50 mg/kg chlortetracycline in basal diet group Ⅱ(origanum oil group, added 20 mg/kg oreganum oil in basal diet group Ⅲ(probiotics group, added 1.5 g/kg probiotics in basal diet group Ⅳ(nutrient enhancement group, elevated dietary vitamin E, vitamin B family and micro elements Zn, Mn, I group Ⅴ(origanum oil+nutrient enhancement group group Ⅵ(probiotics+nutrient enhancement group).
试验选用平均体重27 kg，健康长×大杂交阉公猪324头，随机分为6组：Ⅰ组（金霉素组，在基础日粮中添加50 mg/kg金霉素）、Ⅱ组（牛至油组，在基础日粮中添加20 mg/kg牛至油）、Ⅲ组（益生素组，在基础日粮中添加1.5 g/kg益生素）、Ⅳ组（营养调控组，提高日粮中维生素E和B族维生素、微量元素Zn、Mn和I水平）、Ⅴ组、Ⅵ组。
Resistance in Bodao, Tieganqing or Queernuo to blast Hoku 1 might be controlled by a dominant gene, and in Jiangnanwan by two inhibiting effect genes. Resistance in Tieganqing or Queernuo to blast ZE3 might be controlled by one dominant gene, and in Bodao and Jiangnanwan by two independently dominant genes and two inhibiting effect genes, respectively. Resistance in Tieganqing to blast ZG1 might be controlled by a dominant gene, but in Bodao and Jiangnanwan by two inhibiting effect genes. The crosses were further made between landrace Bodao and 12 Japanese differential varieties possessing the known resistance genes to the blast to produce F1 and F2 generations.
结果表明：薄稻、铁杆青及缺儿糯对北1菌系的抗性均可能由一对显性基因控制，江南晚对北1的抗性则可能由两对抑制基因互作控制；铁杆青及缺儿糯对ZE3小种的抗性均可能由一对显性基因控制，薄稻和江南晚对ZE3小种的抗性可能分别由两对显性基因和两对抑制基因互作控制；铁杆青对ZG1小种的抗性可能是由一对显性主基因控制，薄稻和江南晚对ZG1小种的抗性则可能由两对抑制基因互作控制。
Polymorphic sites were found only in Yorkshire, Landrace, Duroc and their hybrids, but that were not found in Mm pig and wild pig.
仅在大白猪、长白猪，杜洛克和杂种猪中发现多态性位点，而在民猪和野猪中未发现。
Healthy Landrace × Rongchang crossbred piglets (initial body weight about 15kg) were allotted randomly into five treatments. The control treatment consisted of 4 replicates and each of the other treatment consisted of 5 replicates with one piglet per replicate.
消化试验选用24头健康、体重约15kg的约克×荣昌仔猪，随机设5个处理，对照组4个重复，其他处理组5个重复，每个重复1头猪。
Via single factor experiments of feed on 40 pigs crossed from Landrace, Large Yorkshire and leping pig.
采用不同饲粮配方单因子试验设计，以40头长大乐三元杂种猪为素材，在相同条件下，系统对猪的肥育、屠宰性能以及经济效益进行了研究。
In this paper, full sequence of porcine growth hormone gene was amplified in Large Yorkshire and Landrace by PCR. The amplification products were analyzed in sequence polymorphisms. BstXI, Apal, Hhal and Mspl polymorphisms were detected. Effects of different genotypes on some important production performance were analyzed according to the acquired data.
本试验以大约克猪和长白猪为研究对象，利用PCR技术扩增了猪生长激素基因全序列，并对PCR产物进行了序列分析，以及对BstⅪ、ApaⅠ、HhaⅠ和MspⅠ 4种内切酶酶切位点多态性进行了研究，同时结合性能测定资料，分析了不同基因型或带型与生产性状的关系。
In this experiment, polymorphism of serum protein, including transferring, prealbumin, postalbumin, amylase, ceruloplasmin and esterase, of Large White, Landrace, Duroc and SD-III of Shanxi lean meat type swine was investigated by using vertical polyacrylamide gel electrophoresis.
本试验采用垂直板聚丙烯凝胶电泳法，对大白猪、长白猪、杜洛克猪和山西瘦肉型猪SD-Ⅲ系4 种猪群的血清转铁蛋白、血清前白蛋白、血清后白蛋白、血清淀粉酶、血清铜蓝蛋白和血清酯酶的多态性进行了研究。
The results show that: pig species include Large White, Landrace, Duroc pigs and swine; cattle varieties of Simmental, Shorthorn and Brahman cattle, of Jersey cattle; sheep varieties Boer goats; duck, duck varieties, Cherry Valley Duck, Shaoxing Duck; Chicken varieties are emerging sanhuang, Guangxi top soil chicken, Ma top emerging chicken, black plume bamboo silk chickens and other species.
研究结果表明：（1）猪的品种主要包括大白猪、长白猪、杜洛克猪和香猪；牛的品种有西门塔尔牛、短角牛、婆罗门牛、娟姗牛；羊的品种有波尔山羊；鸭的品种有北京鸭、樱桃谷肉鸭、绍兴麻鸭；鸡的品种有新兴三黄鸡、广西土顶鸡、新兴麻顶鸡、黑羽竹丝鸡等品种。
Twelve Duroc × Landrace × Yordshire crossbred piglets with 8.8±1.0 kg bw weaned at 28±2 d were randomly assigned into four groups and fed with four iso-nitrogen, iso-energy and iso-starch diets formulated with corn, brown rice, stick and RS as starch sources respectively.
选择（28 2）d断奶的杜长大三元杂交仔猪（平均体重8.8kg 1.0kg）12头，随机分为4组，分别饲喂以玉米、早籼稻糙米、糯米和Rs为淀粉来源配制的4种等能、等氮、等淀粉试验饲料，饲养25d后，测定不同饲料淀粉来源对断奶仔猪血糖、胰岛素、GH、T3和T4水平的影响。
To Landrace, Yorkshire and Duroc, LY (the hybried of Landrace boar and Yorkshire sow), YL (the hybried of Yorkshire boar and Landrace sow) adopts artificial insemination and nature service two methods, and at swine artificial insemination use admixture semen method service sows also, study the influences of different species import swine's fertility rates and breeding function circumstance, compare the influences of import swine's fertility rates, nest average piglet number, piglet individual born heavy and nest heavy after adopted artificial insemination technique, and measured the semen collection records of Landrace, Yorkshire and Duroc boar, and discussed how to judge swine heat and service on time at apply and expansion swine artificial insemination technique, how to teaching boar and semen collection, artificial insemination effictiveness analyse, and how to extend the swine artificial insemination technology ect., and analyze the influence of sow fertility rates, piglet counts when use the same species and different species boar semen at the artificial insemination.
本文对长白、大约克、杜洛克及长大、大长母猪采用人工授精及自然交配两种配种方式，同时还在人工授精配种方式中应用混合精液方式输精，研究了不同配种方式对外种母猪情期受胎率和繁殖性能的影响情况，比较了不同品种外种猪采用人工授精技术后对母猪情期受胎率、窝平均产仔数、仔猪初生个体重及窝重的影响，同时对长白、大约克、杜洛克三个品种的采精量作了统计测定，讨论了外种猪人工授精技术应用与推广中母猪的发情鉴定与适时输精、公猪调教及采精、人工授精效果分析及其技术推广，分析了用同品种与不同品种公猪精液进行人工授精对母猪受胎率和产仔数的影响情况。
Clustering results show that jihebai A combined with jihebai B first, and then combined with the Large White, with the Liaoning Black and Tanyu Black together; combined with Duroc and Landrace in turn.
聚类结果表明，冀合白A与冀合白B聚合在一起，然后与大白猪聚在一起，再与辽宁黑猪和丹育黑猪聚在一起；杜洛克、长白猪与他们依次聚在一起。
For carcass traits, significant effects were observed for this locus on BFT1 and BFT2; For meat quality traits, this locus was near to significantly associated with pH and pH and seemed to be dominant in action.(5) LMCD1 gene was highly expressed in heart and muscle while it had a broad expression spectrum across all tissues examined.3、NRAP (Nebulin-related anchoring protein):(1) The full coding sequence of LargeWhite, Landrace and Meishan breeds were isolated. The nucleotide sequence of 5'RACE (rapid amplification of cDNA end) and intron 39 and 40 have been deposited to GenBank under accession number DQ157553 and DQ480149.(2) 35 SNPs were identified by comparing sequences, three of which resulted in amino acid change.(3) PCR-Eco721-RFLP was developed to detect A/G substitution in intron 39. In unrelated pigs from eight breeds, both allele A and B were found in Chinese pig breeds, while only B was in LargeWhite and Landrace.(4) Association analysis in 302 F_2 animals showed significant associations between Eco721-RFLP genotypes with BWT, CFW, LEH and BFT1 for carcass traits and pH, pH, MCV1, MCV2, DLR and WHC for meat quality traits. This locus was predominant in dominance for carcass trait, but in addition for meat quality.(5) NRAP mRNA was transcribed especially in heart and skeleton muscle.4、ECH1 (Enoyl CoA hydratase 1):(1) The full coding sequence of LargeWhite, Landrace and Meishan breeds, 5'RACE and all introns except intron 3 were isolated and deposited to GenBank under accession number DQ157552 and DQ480146DQ157552.(2) 17 SNPs were identified, of which one was cSNP.(3) Two A/G substitution were detected by PCR-BamHⅠ-RFLP in intron 1 and PCR- PstⅠ-RFLP in intron 5 separately, and T/C mutation in exon 4 was detected by PCR-SSCP. Among seven different pig breeds examined, results of BamHⅠ-RFLP in intron 1 showed that the frequency of allele B was near to 1 in LargeWhite and Landrace; both allele A and B were found in Chinese pig breeds, and allele B was predominant in other breeds except Meishan and Qingping. For PCR- PstⅠ-RFLP in intron 5, in seven commercial pig breeds, both alleles C and D were detected in all other examined breeds except Jianli pig which only had allele D.(4) Association analysis in 296 F_2 animals showed significant associations between BamHⅠ-RFLP genotypes and CFW, LEH and WM. And this locus seemed to be dominant in action. For PCR- PstⅠ-RFLP in intron 5 in 306 F_2 animals, for carcass traits, significant effects were observed for this locus on DP and AST; For meat quality traits, this locus was significantly associated with MCV2.(5) Expression of ECH1 gene can be detected in all tissues examined: heart, liver, lung, kidney, spleen, stomach, small intestine, muscle and fat.5、CKMT2 (creatine kinase, mitochondrial 2):(1) The full coding sequence of LargeWhite, Landrace and Meishan breeds were isolated and deposited GenBank under accession number DQ363337. In addition, the sequence of intron 4 can be seen in appendix 14.(2) Of nine SNPs identified within these sequences, three of which resulted in amino acid change.(3) An PCR- MspⅠ-RFLP was developed to detect the A/G polymorphism in intron 4. Among nine pig breeds examined, allele B was 1 or near to 1 in Meishan and Erhualian, while in other seven breeds all were 1 of allele A.(4) 302 F_2 animals were typed for association analysis. For carcass traits, significant effects were observed for this locus on BWT, IFR, FMP, CL, BFT1, BFT2 and BFT3, and their additive effects were predominant; For meat quality traits, this locus was significantly associated with WM.(5) Expression of CKMT2 gene was predominant in heart and skeleton muscle and moderate in lung, kidney and fat, although a weak signal was observed for liver, ovary and testis, and no expression in spleen, stomach, small intestine and uterus.6、TTID (Titin Immunoglobulin Domain Protein):(1) The full coding sequence of LargeWhite, Landrace and Meishan breeds and sequences of all introns except intron 2 and 7 were isolated and deposited to GenBank under accession number DQ157551 and DQ480148.(2) Total 51 SNPs within these intron sequences and no mutation or deletion was found in cDNA sequences.(3) An PCR- HinfⅠ-RFLP was developed to detect T/C substitution in intron 6. Among ten pig breeds which were typed by HinfⅠ-RFLP, results showed that there were significantly different in allele frequencies between Chinese indigenous pig breeds and foreign pig breeds: the frequencies were 1 or near to1 in LargeWhite and Landrace, while allele A was predominant in other five breeds although allele B were only a bit dominant in Erxihei, Qinsping and Bamei.(4) 279 F_2 animals were typed for association analysis. For carcass Waits, significant effects were observed for this locus on SP, LEA and AST, and their additive effects were significant; For meat quality traits, this locus was significantly associated with pH, MCV1 and WM, and their additive effects were significant.(5) Expression of TTID gene was highest in skeleton muscle and heart, with weak level in fat and lung, and no signal in other tissues.7、PFKM (Phosphofructokinase Muscle Type):(1) The full cDNA sequence of LargeWhite, Landrace and Meishan breeds and sequences of 2-21 introns were isolated and deposited to GenBank under accession number DQ363336 and DQ480147. And the length of sequence of intron 1 was identified.(2) Of the 111 SNPs identified within all sequences, four of which resulted in amino acid change.(3) PCR-TaqⅠ-RFLP were developed to detect T/C substitution in exon 13 and exon 17. In unrelated individuals from seven different pig breeds, allele frequencies of the TaqⅠ-RFLP in exon 13 was compared: frequencies of allele B in LargeWhite, Landrace, Erxihei were 1 or near to 1, and a little dominant of allele B in other four Chinese pig breeds. For the same polymorphism in exon 17, the trend of allele frequencies distribution was the similar to that of exon 13.(4) For association analysis in the 214 F_2 animals, significant effects were observed for different genetype of exon 13 on BWT, IFR, FMP, LMP, RLF, LEH, BFT1, BFI2, BFT4 and ABF of carcass traits. It is notable that both additive effects and dominant effects in BWT, LMP, RLF and LEH were very significant; For meat quality traits, this locus was significantly associated with MCV2, MM1, WM and IMF. Association analysis in 241 F2 animals showed there were significant effects on BFI3 and ABF of carcass traits and on MM1, MM2, DLR, WHC, IMF and WM of meat quality traits with TaqⅠ-RFLP in exon 17.(5) Expression of PFKM gene had different levels in each examined tissue except stomach and small intestine, with highest in skeleton muscle, then in heart.8、MYLPF (myosin light chain, phosphorylatable, fast skeletal muscle):(1) The full genomic sequence of of LargeWhite, Landrace and Meishan breeds were isolated and deposited to GenBank under accession number DQ533994.(2)43 SNPs, including 5 cSNPs, were found by comparing sequences of three pig breeds, three of which resulted in amino acid change.(3) PCR-TaqⅠ-RFLP and PCR-MspⅠ-RFLP were developed to detect G/A substitution and T/C substitution in intron 1 separately. For MspⅠ-RFLP among seven different pig breeds examined, the frequency of allele B was two times of that of allele A in Landrace, while allele B were predominant in other breeds. For TaqⅠ-RFLP, the frequency of allele B was two times of that of allele A in LargeWhite and Landrace.(4) The locus of MspⅠ-RFLP was typed in 140 pigs (Landrace, LargeWhite, Meishan, LargeWhite×Landrace and Landrace×Large White pigs) to study the association between carders of different genotype and the trait values. For carcass traits, significant effects were observed for this locus on LMP, SP, LEW and LEA, with approximative significant on BFT1 and LEH(P＜0.06). This locus appeared to be additive effects with different action direction; For meat quality traits, this locus was significantly associated with MM1, MM2 and WM and IMF, which appeared to be predominant on addictive effects.(5) RT-PCR analysis of MYLPF showed its expression in all tissues examined, with highest levels in heart and skeleton muscle.
在所检测的6个猪种中，大白、长白、鄂西黑和皖南花猪种G等位基因的基因频率为1或接近1，而梅山和清平品种的等位基因G基因频率分别为0.2889和0；(4)在178头大×梅F_2代资源家系中进行标记性状关联分析，该位点与胸腰椎间背膘厚和臀部平均背膘厚差异显著，该位点以显性作用方式为主，且方向一致；(5)该基因在所检测的各组织中均有表达，在心脏和肌肉中表达量最高。3、NRAP(Nebulin-related anchoring protein，伴肌动蛋白相关锚定蛋白)：(1)获得了大白猪、杜洛克猪、梅山猪三个猪种基因编码序列、5`RACE(rapid amplificationof cDNA end，快速扩增cDNA末端)及其第39、40内含子序列，序列已提交到GenBank中，登录号为DQ157553和DQ480149；(2)发现了35个SNPs，其中3处预测可导致氨基酸改变；(3)建立了第39内含子A／G替换的PCR-Eco721-RFLP分型方法；在所检测的8个猪种中，大白、长白猪种B等位基因的基因频率为1，中国地方猪群可检测到A和B等位基因；(4)在302头大×梅F_2代资源家系中进行标记性状关联分析，该位点与出生重、内脂率、眼肌高度、肩部背膘厚、背最长肌pH值、失水力、系水力、背最长肌肌肉色值、股二头肌pH值和股二头肌色值显著或极显著相关，该位点在胴体性状以显性作用方式为主，肉质性状则以加性作用为主；(5)该基因特异性的在心脏和骨骼肌中表达。4、ECHI (Enoyl CoA hydramse 1，烯酯酰辅酶A水合酶1)：(1)获得了大白猪、长白猪、梅山猪三个猪种基因编码序列、5`RACE及第3内含子外的所有内含子序列，并提交到序列的GenBank登录号为DQ157552和DQ480146；(2)序列比对发现了17个SNPs，其中1处cSNP；(3)建立了第1内含子A／G替换的PCR-BamHⅠ-RFLP、第5内含子A／G替换的PCR-PstⅠ-RFLP分型方法和第4外显子T／C突变的PCR-SSCP分型方法；第1内含子的酶切多态，在所检测的7个猪种中，大白和长白猪种B等位基因的基因频率接近为1，而中国猪种均可检测到A和B等位基因，除清平猪种外，其余品种均为B等位基因占优势；关于第5内含子多态性分布，在所检测的7个猪种中，监利猪未检测到A等位基因，其余猪种均可检测到A和B等位基因，均为B等位基因占优势；(4)在296头大×梅F_2代资源家系中进行标记性状关联分析，第1内含子多态的不同基因型间内脂率、眼肌高度和肌内水分等生产性状差异显著，以显性效应为主；在306头大×梅F_2代资源家系中进行标记性状关联分析，第5内含子位点与屠宰率、平均皮厚和股二头肌色值差异显著；(5)该基因在所检测的9个组织中均表达。5、CKMT2 crcatine kinase，mitochondrial 2(sarcomeric，肌节线粒体肌酸激酶2：(1)获得了大白猪、长白猪、梅山猪三个猪种基因cDNA序列，该基因GenBank登录号为DQ363337，获得的第4内含子序列见附录14；(2)序列比对，发现了9个SNPs，其中3处预测可导致氨基酸改变；(3)建立了第4内含子A／G替换的PCR-MspⅠ-RFLP分型方法，在检测的9个猪种中，梅山和二花脸猪中B等位基因频率为1或接近1，其他7个猪种均为A等位基因频率为1；(4)在302头大×梅F_2代资源家系中进行标记性状关联分析，该位点与出生重、内脂率、肥肉率、胴体长、肩部背膘厚、胸腰椎间背膘厚、臀部背膘厚和肌内水分等性状差异显著，以加性作用方式为主；(5)该基因在心脏和骨骼肌中表达丰度最高，在脾脏、胃、小肠和子宫中不表达，在其余组织不同程度的表达。6、TTID (Titin Immunoglobul in Domain Protein；肌联蛋白免疫球蛋白区域蛋白)：(1)获得了大白猪、长白猪、梅山猪三个猪种基因全长cDNA序列以及除第2、7内含子外的所有内含子序列，提交到GenBank的登录号为DQ157551和DQ480148；(2)序列比对发现了51个SNPs，该基因cDNA中没有发现碱基突变或缺失；(3)建立了第6内含子T／C替换的PCR-HinfⅠ-RFLP分型方法，在检测的10个猪种中，在大白和长白猪种中B等位基因的频率为1或是接近1，国内品种中除鄂西黑猪、清平猪和八眉猪中B等位基因频率略占优势外，其余5个品种均为A等位基因占绝对优势；(4)在279头大×梅F_2代资源家系中进行标记性状关联分析，胴体性状方面，该位点与皮率和眼肌面积差异显著，与平均皮厚差异极显著；并且表现为加性效应显著或极显著，肉质方面，该位点与股二头肌pH值、背最长肌和肌内水分差异显著，表现为显性效应显著；(5)该基因在心脏和骨骼肌中表达量很高，在脂肪和肺中微量表达，其余组织中不表达。7、PFKM (Phosphofructokinase Muscle Type，肌型磷酸果糖激酶)：(1)获得了大白猪、长白猪、梅山猪三个猪种基因全长cDNA序列及第2到21内含子序列，验证猪中第1内含子的大小，序列GenBank的登录号为DQ363336和DQ480147；(2)序列比对发现了111处SNPs，其中4处预测可导致氨基酸改变；(3)建立了第13外显子T／C替换和第17外显子的PCR-TaqⅠ-RFLP分型方法，在所检测的7个猪种中，第13外显子多态性分布频率在检测的大白、长白和鄂西黑猪中B等位基因频率为1，另外四个国内猪种均为B等位基因占优势，在所检测的7个猪种中，第17外显子多态性分布频率基本同第13外显子位点的分布，(4)在214头大×梅F_2代资源家系中进行标记性状关联分析，第13外显子位点基因型不同时，出生重、内脂率、肥肉率、瘦肉率、瘦肥比率、眼肌高度、6—7胸腰椎间背膘厚、胸腰椎间背膘厚、肩部背膘厚、三点平均背膘厚、股二头肌色值、背最长肌大理石纹、肌内水分和肌内脂肪差异显著或极显著；该位点在部分胴体性状加性效应和显性效应均为极显著；在241头大×梅F_2代资源家系中进行标记性状关联分析，第17外显子位点与眼肌高度、臀部背膘厚、三点平均背膘厚、大理石纹、失水率、系水力、肌内脂肪和肌内水分差异显著或极显著；(5)该基因在心脏和骨骼肌中表达量很高，胃和小肠没有表达，其他组织均有不同程度的表达。8、MYLPF (myosin light chain，phosphorylatable，fast skeletal muscle，肌浆球蛋白轻链可磷酸化蛋白)：(1)获得了大白猪、长白猪和梅山猪的基因组序列；序列GenBank的登录号为DQ533994；(2)序列比对，发现了43处SNPs，有5处cSNPs，其中3处预测可导致氨基酸改变；(3)建立了第1内含子G／A替换的PCR-TaqⅠ-RFLP和TIC替换的PCR-MspⅠ-RFLP分型方法；在所检测的7个猪种中，第1内含子MspⅠ-RFLP多态性分布频率在长白猪中A等位基因与B等位基因频率的比例大约为1：2，其余猪种为B等位基因占绝对优势；在所检测的7个猪种中，第1内含子TaqⅠ-RFLP多态性分布频率，在大白和长白猪中A等位基因与B等位基因频率的比例大约为1：2，国内猪种均为B等位基因占绝对优势；(4)在140头试验猪群(长白、梅山、长白、长白×大白、大白×长白)中进行标记性状关联分析，基因型不同时，瘦肉率、皮率、股二头肌大理石纹、背最长肌大理石纹和肌内水分差异显著，眼肌宽度、眼肌面积和肌内脂肪差异极显著，肩部背膘厚和眼肌高度差异接近显著水平；该位点在这些性状上均表现为加性作用方式，但作用方向不同；(5)该基因在各组织中均有表达，尤以心脏和骨骼肌中表达丰度最高。
In this Study., The Polymorphisms of porcine Pit-1 partial region in four breeds pigs including Erhualian, Meishan, landrace and Yorkshare were investigated. A pair of primers were used to amplify a 1746 bp DNA fragment which started from exon5 to the end of exon6, then two enzymes were used to digest this PCR product respectively and detected its polymorphism. We found that Pst I enzyme produced four fragments (811bp 570bp 546bp and 360bp) and three genotypies, while Rsa I enzyme produced 736bp and 710bp or (388bp and 322bp) fragments and three genotypies including EE, EF, FF.
本试验以梅山猪、大约克猪、二花脸猪、长白猪为实验动物，采取特定引物扩增Pit-1基因的exon 5到exon6之间的长度为1746 bp的DNA片段，应用PCR-RFLP技术，利用Pst Ⅰ、Rsa Ⅰ二种内切酶酶切PCR产物，PstⅠ酶切产物为811bp、570 bp或546bp、360bp片段，基因型为AA、AB、BB，Rsa Ⅰ酶切产物为736bp、710bp或(388bp与322bp)基因型为EE、EF、FF，Pst Ⅰ酶切产物中出现一个24bp小片段链缺失，这个缺失将有重要的遗传多态分析价值。
In this Study., The Polymorphisms of porcine Pit-1 partial region in four breeds pigs including Erhualian, Meishan, landrace and Yorkshare were investigated. A pair of primers were used to amplify a 1746 bp DNA fragment which started from exon5 to the end of exon6, then two enzymes were used to digest this PCR product respectively and detected it's polymorphism. We found that Pst I enzyme produced four fragments (811bp 570bp 546bp and 360bp) and three genotypies, while Rsa I enzyme produced 736bp and 710bp or (388bp and 322bp) fragments and three genotypies including EE, EF, FF.
本试验以梅山猪、大约克猪、二花脸猪、长白猪为实验动物，采取特定引物扩增Pit-1基因的exon 5到exon6之间的长度为1746 bp的DNA片段，应用PCR-RFLP技术，利用Pst Ⅰ、Rsa Ⅰ二种内切酶酶切PCR产物，PstⅠ酶切产物为811bp、570 bp或546bp、360bp片段，基因型为AA、AB、BB，Rsa Ⅰ酶切产物为736bp、710bp或(388bp与322bp)基因型为EE、EF、FF，Pst Ⅰ酶切产物中出现一个24bp小片段链缺失，这个缺失将有重要的遗传多态分析价值。
The study was to determine the effects of kitasamycin, virginiamycin and galacto-mannan-oligosaccharides on serum biochemical indices of lactating sows. Twenty-four sows (Landrace×Yorkshine) were randomly divided into four groups with six replicates of each.
摘 要：将24头长×大二元母猪随机分为4组，每组6头，以研究基础日粮添加吉它霉素、维吉尼亚霉素和半乳甘露寡糖对其泌乳期结束时血清生化指标的影响。
A resource population constructed by F 2 design with Landrace and Chinese indigenous Lantang pigs was used in this study.
数量性状是由少数效应较大的主基因或数量性状座位和许多效应很小的多基因控制的[1] 。
Lantang pig (16 sows) which is one of south China type pig breeds, were crossed with Landrace pig (8 boars) to construct the resource population. According to the pig linkage map of USDA-MARC2.0, 31 microsatellite DNA markers on pig chromosomes 1, 4, 7, and 8 were used to genotype the parents F1 and F2. The distance between adjacent markers was about 10 to 20 cM. The gene frequence, heterozygosity, and polymorphism information content were calculated. The marker genotype of parents, F1, and F2 were obtained with WAVE nucleotide fragment analysis system and ABI 377 DNA sequencer.
利用中国地方猪种蓝塘猪（16头母猪）与外来品种长白猪（8头公猪）按F2设计建立资源家系，根据美国肉畜中心(USDA-MARC2.0)公布的猪连锁图谱，在1、4、7和8号染色体上间隔10~20 cM选择一个微卫星标记，共31个标记，采用WAVE核普酸片段分析系统和ABI 377 DNA序列分析仪检测资源群体的P、F1和F2代个体微卫星的基因型，对其基因频率、杂合度和多态信息含量等进行统计分析。