Cytopathic effect was characteristic of cell rounding, congregating and detachingfrom the cellular sheets, was produced in infected cells.
病毒血凝价的改变与跨种感染的关系据报道IBV经胰酶或磷酸脂酶C处理后可以凝集0.5％的新鲜制备的鸡红细胞。
However, the strains M41, H52, H120 andGray, but not Holte, Connecticut and M52-19, could propagate in freshly digested HeLacells.
然而这唯一的一个点突变对IBV的跨种感染似乎没有决定性的作用。
Sequences analysis showed: S1, M, N gene of strain HaN2-95 all had the highest similarities with that of IBV H120 vaccine strain; S1 gene of strain HaN1-95 had the closest genetic relationship with IBV H52 vaccine strain, while the M, N gene of strain HaN1-95 had the highest similarities with that of strain Gray; although the S1 and M gene of strain GX1-98 all had the highest genetic relationship with that of IBV H52 vaccine strain, yet the N gene of strain GX1-98 had high similarity with that of IBV Ark99 strain or Gray strain; S1 gene of GX2-98 strain showed the high similarities with that of IBV Holte strain.
序列分析结果表明：HaN2-95株的S1、M和N基因核苷酸序列均与IBV H120疫苗株的同源性最高；尽管HaN1-95株的S1基因与IBV H52疫苗株的亲缘关系最近，但是该毒株的M基因和N基因却与IBV Gray株的同源性最高；GX1-98株的S1和M基因均与IBV H52疫苗株的亲缘关系最近，但其N基因却与IBV Gray株和Ark99株有高度的同源性；GX2-98株的S1基因却与IBV Holte株的亲缘关系最近。
Themain projects are: 1: IBV propagation in HeLa cells Allantoic fluid of IBV strains including M41, H52, H120, Gray, Holte, Connecticutand M52-19 (a Beaudette52-19 strain) containing IBV 5×10~(304) EID50 was inoculated intoconfluent HeLa monolayers or freshly digested HeLa with 0.25% trypsin. All of them could not propagate in HeLa cell monolayers.
S1基因的变异在IBV跨种感染过程中的作用 M41毒株在新鲜消化的HeLa细胞上持续传26代，RT-PCR扩增第1代、第5代和第21代病毒的S1基因，结果显示第1代跟第5代S1基因的序列完全一致，而第21代与第1代相比也仅仅只有一个碱基(A728G)的改变，导致一个氨基酸的变异。
The repeatability of test was good and the microarray detection technique was verified for its specificity, sensitivity and repeatability. The microarray can be stored at room temperature for at least 3 months.
试验结果为NDV-IBV检测基因芯片与鸡痘病毒、鸡毒支原体、鸡减蛋下降综合症病毒、鸡大肠杆菌、鸡沙门氏杆菌等无交叉杂交，探针最小使用量为0.44ng/μl，表明该芯片具有高特异性、高灵敏性；批间芯片、批内芯片和同一芯片重复利用均有良好的重复性；芯片在室温下至少可保存3个月。
The amount of target protein shares 12.5% of the total protein which consist in the supernatant fluid of yeast culture medium.
表达的IBV S1蛋白可作为制备IB诊断抗原、基因工程疫苗的基础材料。
In addition, CK/CH/LDL/97 I strain was attenuated by serial 115 passages in embryonated chicken eggs (note: the 115th passage were completed in our laboratory by prof. Rong Jun-gong).
同时将IBV CK/CH／LDL97Ⅰ／97株通过鸡胚连续传115代致弱成一株免疫原性良好的疫苗株(备注：F115的鸡胚传代由本实验室荣骏弓研究员完成)。
Additionally, the TW I attenuated vaccine strain had no reversion to virulence after five back passages in chicks. Therefore, these two IBV strains were transferred to a manufacturer for the vaccine production and evaluation. The safety and the efficacy of this attenuated strain were also tested in SPF chickens.
因此将此台湾一型及台湾二型毒株交付厂商制成冷冻乾燥疫苗后进行更进一步的测试，包括病毒力价测定、安全试验、抗体力价试验及攻毒试验，结果显示前者102.3、103.3、104.3 EID50/只的免疫剂量对3至10日龄小鸡皆不具有致病性。
In the process of reproduce, E protein takes part in the formation of VLP and budding of the whole virus.
在病毒的复制过程中，E蛋白参与VLP形成和病毒出芽，近来又发现E蛋白在IBV感染后期的细胞中非常丰富，推测该蛋白与细胞凋亡有关。
The results showed that different neutralization temperatures had significant effects on the EID50 of IBV and the high temperature(37 ℃) led to a remarkable decline in titre of IBV.
结果显示，中和温度对该二联活疫苗中鸡传染性支气管炎病毒的EID50有显著的影响。测定病毒含量时，中和条件以中和温度控制在20℃、中和时间45～60min较为合适。
Since 1995, more than 100 virus isolates were obtained from proventriculus of the chickens infected with TP. 13 isolates were identified by EM observation and chicken embryo propagation as IBV.
1995年以来，先后从临床上表现TP的病鸡腺胃组织内分离到100多株病毒，选择其中的13株进行了电镜观察和鸡胚传代，初步鉴定为IBV。
Virual RNAs extracted from blood, spleen and cerebrum were lablled with CY3 by one-step RT-PCR. The test results indicated that detection rate of IBV and NDV reached above 90%.
试验以试剂盒方法从血液、脾脏和大脑中提取病毒RNA，一步法RT-PCR扩增标记，杂交检测分析表明，IBV和NDV试验鸡病料检出阳性率可分别达90％以上。
In our test, the mutation in S1 gene seemed notthe major reasons responsible for IBV tropism switch. 3: Relationship between HA activity and cross-species infection of IBV After treated with lecithinase C, the strains M41, H52, H120 and Gray, but notHolte, Connecticut and M52-19, could haemagglutinate 0.5% chicken erythrocytes.
唾液酸糖蛋白在IBV跨种感染过程中的作用某些冠状病毒可以利用细胞表面的唾液酸糖蛋白作为进入细胞的受体，而神经氨酸酶可以降解细胞表面的唾液酸糖蛋白，这样就会降低病毒感染的几率。
The results provided foundation for further studies on structure and function of nucleocapsid gene and development of the IBV genetic engineered vaccine.
研究结果为进一步研究IBV核衣壳蛋白的结构与功能以及基因工程疫苗的研制奠定了基础。
A pair of primers were designed according to isolated IBV's S1 gene sequences which were accepted by Genbank and the sequence of Pichia pastoris expression vector pPIC9K's Multiple Cloning Site. Then the S1 gene was amplified by RT-PCR, The amplified products were cloned and sequenced.
本研究根据本课题组在GeneBank中注册的禽传染性支气管炎病毒中国分离株的S1基因序列(AF397527、AF397528、AF397529和AF036937)及毕赤酵母分泌型表达载体pPIC9K的序列设计引物，用RT-PCR方法扩增出IBV S1基因。
The results showed that these were major variance in the N gene of 793/B.
表明IBV 93/B的N基因存在着较大的变异性。
The re was no significant difference between the contents of SP and VIP of lung tissue in experiment group and those in control group.4. Ultrastructurally, after infected with IBV, lots of grume were observed on the surface of trachea tissue and the cilia of the trachea were massed.
免疫电镜观察结果显示，大多数肾小管上皮细胞胞浆内均见有SP及VIP阳性颗粒，在上皮细胞间还可见有DNES细胞；肺脏中异嗜性白细胞颗粒也常呈SP及VIP阳性反应，肺脏内也可见DNES细胞。
In addition to clinical diagnosis, this technique was also applied to the IBV detection in samples from two slaughter houses. Besides one vaccine isolate, twelve flocks showed positive IBV isolation from 82 chicken flocks (14.6%), including eleven TW I isolates and one novel genotypic isolate, but no TW II isolate.
除了临床诊断外，此项技术还应用在两座屠宰场鸡只样本的IBV检测上，结果显示在总共82个鸡群中，一共分离出1个疫苗毒株，以及其他12个IBV分离株，其中包含11个台湾一型分离株，及一个未确定基因型毒株，但无台湾二型分离株。
Every virus strain were studied with chicken recurrent infection, biological characteristics assay, seroneutralization test, localization of IBV antigens, amplification of S1 gene and nucleic acid sequence analysis.
从天津、河南、北京的鸡场分别分离到TJ9602、HN9604、BJ9601鸡传染性支气管炎病毒毒株，对其进行了动物回归、生物学特性、理化特性、血清学试验、抗原定位，对分离株的S1基因进行了RT-PCR扩增、核酸序列分析等研究。
Virus was also cultured in allantoic cavity of chicken embryo with statistically significant HA titer at 1:6.8 as compared with virus control group (P.01) incubated 24 hours after injected with MEK first then injected with IBV.
在鸡胚尿囊腔中，先注入MEK孵育24 h后，再注入B型流感病毒的鸡胚也培养出病毒，HA滴度为1：6.8，与病毒对照组比较P.01，有统计学意义。