Objective To establish the cultural system for hairy root of Hyoscyamus niger.
目的建立莨菪Hyoscyamus niger的发根培养体系。
Hairy root cultures of Taxus plants, roots of hydroponically grown Taxus plants and nodule cultures of Taxus plants are mainly introduced about the enlargements of cultural materials.
同时在毛状根培养、冠瘿瘤组织培养、水培根培养和瘤培养等培养材料的扩展方面，对国内外所作的尝试性研究和新的报道进行了综述。
We intended toselecting lines containing the high content of PAP from the organ of pokeweed by technology of tissue culture and the technology of hairy root transformation.
商陆抗病毒蛋白在抗植物病毒方面，具在有广谱性和高效性，即对植物病毒无针对性和在非常低的浓度下也能表现出强烈的抗病毒活性。
The transformation of Ri T DNA was examined through high voltage paper electrophoresis. Results The hairy root was originally obtained from E. prostrata. The result of high voltage paper elctrophoresis confirmed the transformation of T DNA from Ri plasmid to the hairy root.
结果 首次利用发根农杆菌A4，R1601，ATCC15834 3种菌株成功地从墨旱莲中诱志出毛状根；经高压纸电泳检测，墨旱莲毛状根中含有甘露碱，表明Ri质粒的T-DNA已整合进毛状根中。
In hairy root transformation^ have got trans-hairy root.
在现有的试验条件下，以粉红色品系在固体与液体培养基上生长速度快，繁殖率高，商陆抗病毒蛋白含量可能较高，抗病能力较强…总体比较而言，粉红色品系做为大量培养对象比较合理。d 门）在商陆毛状根诱导时，以上部叶片为外植体，菌液OD。。。
With the increase of hormone level added into MS medium, callusing frequencies of type Ⅰ, Ⅱ hairy roots were increased, at the same time, the formation of lateral root from hairy root or callus was inhibited.
随着植物生长物质添加含量的增加，Ⅱ型发状根的愈伤组织化程度也加大，同时也抑制了从发状根或其形成的愈伤组织上形成新的分枝侧根。
When auxin level was increased, callusing frequencies of Type Ⅰ, Ⅱ hairy roots were also increased, while formation of lateral root from hairy root or callus was inhibited.
随着生长激素添加浓度的增加，Ⅰ、Ⅱ型发状根的愈伤组织化程度也加大，同时也抑制了从发状根或其形成的愈伤组织上形成新的分枝侧根。
Result: After cultured for 22 days, transferred in fresh B5 medium having 2 mmol·L-1 hydroquione, the hairy root biotransformated hydroquione into arbutin in 24 hours, the bioconversion rate was 89.0% and the content of arbutin in dry root was 13.0%.
结果 ：培养 22d的人参毛状根更换含底物的B5培养基后，对浓度为 2mmol·L-1的氢醌持续转化 24h ，所合成的熊果苷占干重的 13.0%，转化率达 89.0%。
The results showed that Cd concentrations of lower than 50 μmol/L enhanced the growth of hairy roots, while higher than 100 μmol/L inhibited growth and decreased the number of branched roots, also causing the root tips to become brown and shorter in length.
结果表明，Cd≤50 μmol/L时能促进毛状根生长，而高于100 μmol/L Cd 则抑制毛状根生长，使其侧根根尖变褐和变短，数目减少。
There are three kinds of phenotype of hairy roots in Pueraria lobata which induced by Agrobacterium rhizogene 1601 and grew in vitro on hormone-free solid MS medium: phenotype Ⅰ had numorous lateral roots on both sides of primary root and with marked apical dominance; phenotype Ⅱ had numorous lateral roots on both sides of primary root but without apical dominance, no differences was found between primary root and lateral roots; phenotype Ⅲ had numorous lateral roots just on one side of primary root.
发根农杆菌R1601转化的野葛毛状根在无激素MS固体培养基上生长主要有3种形态：第1类毛状根有明显顶端优势，大量侧根分布于主根两侧，第2类毛状根无顶端优势，大量侧根分布于主根两侧，主根与侧根不易区别，第3类毛状根有明显顶端优势，大量侧根分布于主根一侧，三类毛状根所占比例分别为50％、30％和20％，毛状根的生长速率与形态特征无关。
Objective To compare influence of several biotic elicitors （yeast extract, oligogalacturonides, and fungal elicitor） and abiotic elicitors （Ag^＋, Co^2＋, and a-amino isobutyric acid） on tanshinone production in the hairy root culture of Salvia miltiorrhiza.
目的比较几种生物诱导子酵母提取物、寡半乳糖醛酸、真菌诱导子3和非生物诱导子（Ag^＋、Co^2＋和α-氨基异丁酸）对丹参毛状根培养生产丹参酮的影响。
Objective:To study the effect of different concentration of macroelement, microelement and organic element on the growth and accumulation of tanshinones in hairy root of Salvia miltiorrhiza.
目的：研究不同浓度大量、微量、有机元素对丹参毛状根生长量及丹参酮类成分积累的影响。
The optimum temperature for 1601—L—1 growth was at 25℃. More than 35℃, hairy root growth and cell division were heavenly stopped.
最适合于发根生长的温度为25℃，大于35℃显著地抑制发根的生长，影响发根的根尖细胞的正常分裂。
Hairy root cultures of Astragalus membranaceus were established after roots were induced following infection of aseptic leaves with Agrobacterium rhizogenes strain LBA 9402 and factors affecting their growth were investigated.
用发根农杆菌LBA 9402菌株诱导膜荚黄芪叶外植体建立了毛状根培养系统，比较了不同外界因子对毛状根生长的影响，确定了最适培养条件，并在此基础上用3L、5L、10L体积的容器进行大规模培养实验，培养3周后毛状根生长量可达9.7g／l、10.9g／l、9.8g／l，增长倍数为28-35倍。
Objective: To explore the approach to producing plant secondary metabolites using hairy root culture method.
目的：探索用发根培养法生产植物次生代谢物的途径。
Elicitation has become one of the most effective methods for enhancing secondary metabolite production in hairy root cultures.
目前在毛状根中已有的研究结果表明诱导也成为了提高毛状根中次生代谢产物产量的有效方法。
Hairy roots were induced from tobacco leaves with Agrobacterium rhizogenes LBA1334 harboring agropin type PRil855 and binary vector PIG121HM carrying a kanamycin resistant gene nptll and GUS gene and the good hairy root clones were selected. The root with many root hairs and branches grew vigorously toward all the direction on MS medium without plant hormone and on kanamyain containing medium (km:30mg/L). The structure of the hairy root tip is different from that of ordinary root. Witch was not typical root cap maybe the main cause of losing the geotropism of hairy root. Plantlet regenerated from hairy root when cultured on Ms medium without plant hormone. The nicotine content of the hairy root was little higher than that in the natural roots.
本研究通过发根农杆菌工程菌LBA1334(含有野生型pRi1855质粒和pIG121HM双元载体质粒，编码NPTⅡ基因和GUS基因)转化烟草沙姆逊，获得了具有卡那霉素抗性烟草发状根，并筛选出良好的单克隆株系，该发状根具有典型的发状根特性(多根毛、多分支、在无激素培养基上快速生长)，通过根尖压片分析，烟草发状根不具备典型的根冠；发状根中烟碱含量1.61％稍高于与天然栽培品种母体根的含量(1.5％左右)，发状根向培养液中释放烟碱，释放量最多时占总烟碱量的81.1％；在无激素的培养基上获得了再生植株。
The effect of rare-earth elements Eu(superscript 3+) on biomass and anthraquinone production of two hairy clones DH5c, DH7a and normal root Nor cultures of Rheum palmatum was evaluated after various concentration of Eu(superscript 3+) was administrated into culture medium.
试验研究了不同质量浓度的稀土元素肴Eu(上标 3+对掌叶大黄单克隆毛状根DH5c、DH7a和非转化根Nor生物量、蒽醌化合物合成的影响。
Culm sheaths densely hairy, margins ciliate; root thorns short, robust, dense.
竿箨有密毛，边缘具缘毛；根刺短，粗壮，紧密。6 C。montanus 山香竹
Non-growth associated artemisininsynthesis from hairy root growth and linear relationship betweenbiomass and decreasingconductivity were observed.
计算了生物量对碳、氮源的得率系数，并通过元素分析，建立了毛状根生长的基本计量关系式，从理论上计算出了得率系数及呼吸商。