Ccording to the Ames test specification, TA97, TA98, TA100 and TA102 strains were used and the ammounts of 3 kinds of dust and their mixture with benzopyrene were 5000, 500, 50 and 5 μg/plate, respectively. The number of colony was counted after culturing for 48 h at 37 ℃. SCE test was performed with peripheral blood lymphocytes of healthy men. The ammounts of 3 kinds of dust and their mixture with benzopyrene were 500, 50 and 5 μg/plate, respectively. The chromosome specimen slide was made after culture 72 h at 37 ℃ and the frequency of SCE was counted. There were triplicate plates in each group and negative and positive controls were set up in Ames and SCE tests.
mes试验采用TA97、TA98、TA100和TA102菌株，将3种煤尘颗粒及其与苯并芘混合物各分为5000、500、50和5 μg/皿4个剂量组，并分为加与不加S9两部分，细菌37 ℃培养48 h后计算回变菌落数；SCE试验采用健康成人外周血淋巴细胞，煤尘颗粒及其与苯并芘混合物样品分为500、50和5 μg/皿3个剂量组，标本37 ℃培养72 h后制成染色体并计数染色单体交换频率。2个试验均设阴性和阳性对照组，各剂量组均设3个平行样。
METHODS: MTT calorimetric method, trypin blue exclusion, colony formation and H22-bearing mouse model.
噻唑蓝法、台盼蓝拒染法、集落形成以及H22荷瘤小鼠模型。
Result After the strain was cultured for 24 h in the beef-protein medium, the surface of the colony was rough, the bacteria was opacity, white, gram-positive, rod and it could form spore, which showed that it belonged to bacillus.
结果］该菌株在牛肉膏蛋白培养基上培养24 h后，菌落表面粗糙，不透明白色，革兰氏阳性，杆状，能形成芽孢，表明其属于芽孢杆菌属。
According to the gallus colony stimulating factor(colony-stimulating factor, CSF)cDNA sequences published by GenBank, a pairs of specific primer was designed, the local dorking was injected 100 μg/mL ConA, total RNA was extracted from feeding 24 hours lymphocyte from spleen, then dorking colony stimulating factorcDNA fragment was cloned by RT-PCR technology.
根据GenBank中报道的红原锦鸡集落刺激因子(Colony-stimulatingfactor，CSF)cDNA序列，利用Primer Premier5.0软件设计一对特异性引物，对本地肉鸡注射100μg/mL ConA试剂，饲养24 h后，取脾脏提取淋巴细胞总RNA，利用RT-PCR技术克隆集落刺激因子的cDNA片段。
Growth assay and 3H-TdR incorporation and colony formation in soft agar were used.
通过生长曲线测定、3H-TdR参入试验及软琼脂克隆形成试验，观察反义寡核苷酸对SiHa细胞恶性增殖的影响。
In order to produce 9000 kg·hm-2 gain yield of winter wheat, it is necessary to maintain total photosynthetic area at 10~13 m2·m-2 and non-leaf/leaf area ratio at 1.3~1.7 during flowering stage; and aboveground wheat dry-matter at 19500 kg·hm-2, economic coefficient higher than 0.45, total colony bundle number at 22×103~30×103·hm-2, daily conducting amount at 230 kg·hm-2 and conducting amount per bundle at 1 mg·d-1 during mature stage. The comprehensive analysis of the balance between source and sink of high-yield wheat fields shows that CPCE benefits increasing photosynthetic source of wheat.
在此基础上，提出了实现9 000 kg·hm-2麦田的源-库-流各项指标：开花期总光合面积在10~13 m2·m-2之间，非叶面积与叶面积的比例在1.3~1.7左右；成熟时地上部生物产量在19 500 kg·hm-2左右，经济系数在0.45以上；开花期群体维管束总数在 22× 103~30×103束·h-2之间，日输导量在230 kg·hm-2左右，平均束通量在1 mg·d-1左右。3年间小麦高产田源-流-库平衡的综合分析表明，控株增穗途径在增加小麦光合源方面更具优势。
The mixtures were then plated onto blood agar and colony counts were recorded after 24 h incubation at 37°C.
然后将混合物移植至血琼脂培养基上，37°C 孵化 24小时后记录菌落计数。
Many years over cut h as destroyed the forestry colony and endangered human existence environment.
由于多年来的过量采伐，破坏了森林群落结构，危及人类生存环境。
At t h e precise moment t h at t h ey took wing, a colony of t h e same species t h at my wife was watc h ing near t h e bottom of t h e mountain, also sent its queen on a wedding flig h t.
在它们起飞的同一个精确时刻，由我的妻子负责观察的山脚附近的另一窝同种的蚁族，亦放出它们的蚁后举行飞行婚礼。
The result of Sabouraud medium culture with the temperature of 37℃ as follow: Mycelia had pale yellow spherical sporangium after 24 h; mycelium appeared, the sporangium matured and oval spores released after 72 h; glass utensil was full of colony after 120 h.
经Sabouraud培养基培养观察，37℃，24 h菌丝顶端有淡黄色球形孢子囊； 72 h出现气生菌丝，孢子囊成熟，释放卵圆形孢囊孢子； 120 h后菌落充满平皿。
RESULTS: The PGCs from the genital ridge or the gonad of stage 19 embryos were mulberry-like shape or oval at 48 hours. Cell colony was formed and prunosus was found following periodic acid-Schiff staining at 72 hours.
结果：第19期鸡胚性腺原始生殖细胞体外培养48 h呈桑椹状或者椭圆状，72 h形成细胞集落，过碘酸染色呈紫红色。
When inoculating, the modalities ofcells maybe round, triangle or virgulate; and began to be adhesive for 48h; andformation of colony could be formed on day 4-5; Many cells grew in clonalmanner on day 7, and the cultured cells were characterized by large spindle-shapedappearance by the time of 10 days.
刚接种时细胞形态呈圆形、三角形或棒杆状，48h 后细胞开始贴壁，4-5d 可见有集落形成，第7d 形成多个细胞克隆，第10d 细胞呈长梭形，2w 左右可达到基本融合；传代细胞呈均匀的纺锤形，基本上无悬浮细胞。
The relation between these changes in tumorous tissue and DNA ploidy types, DI value, number of DNA H colony and DNA ploid heterogeneity are very close.
而且这些变化与肿瘤组织倍体类型、DI值、DNA异倍体克隆数以及DNA倍体异质性等均有十分密切的关系。
Preferred to choose 18 h for CFU Count and colony observation, and to choose the researching medium with 0.5% agar as the CFU counting culture medium for Clostridium sporogenes compared with other 5 media.
优选18h进行CFU/mL计数并观察菌落形态，优选琼脂浓度为0.5%的上述培养基作为生孢梭菌CFU计数培养基并与其他5个培养基进行生孢梭菌CFU计数的比较试验。
The favorable pH for its growth, sporulation and germination ranged from 6.2 to 7.0. A photoperiod of 24 h light promoted sporulation and a photoperiod of 24 h darkness favored the growth of the colony.
最适宜生长、产孢和孢子萌发的pH范围为6.2~7.0；24h光照有利于产孢，而24h黑暗有利于菌落生长；
Methods PC-3m and LNCaP were exposed to high temperature at 43 ℃ or 37 ℃ for different duration (1 h or 2 h), and cell survival inhibition rate was evaluated by colony formation assay and measurement of cell growth rate.
方法用成集落实验和细胞生长曲线分析癌细胞在43℃和37℃作用1h或2h后的生长抑制率。
Drt-11 were, the lower rate of germination was. Treating over 24 h in the fermented liquid, the germination rate would reduce by 40% - 60%. And the diameter of colony was 13.9% of CK.
菌核在芽孢杆菌Drt-11发酵液中处理的时间越长，其萌发率越低，在发酵制剂中处理24h后，菌核萌发减少40%~60%，菌落半径是对照的13.9%，明显抑制菌丝的生长。
Methods: The effect of YS2H on bone marrow cells from 25 patients of AML with 3H TdR and colony assay were.
方法：采用白血病祖细胞集落生长方法、3H-TdR掺入法，观察YS2H对25例急性髓细胞白血病患者的骨髓白血病细胞作用；
Methods: K562 cells were dealed with different concentrations for 24h, 48, 72 hours in the culture of liquid, semi solid colony. And the effect of GPT's proliferation and inhibition were observed by cell growth curve and colony forming rate.
方法：在液体培养、半固体集落形成培养中，以不同浓度的GPT处理K562细胞24、48、72小时，用细胞生长曲线和集落形成率来观察GPT的增殖和抑制效应；
Synthesis of DNA in the transferred GLC-82 Cells was inhibited in~3H-TdR incorporation test; the rate of colony-forming decreased in soft agar culture and the tumorigenicity in nude mice decreased.
3HTDR掺入试验结果提示野生型p53基因能够抑制GLC82细胞的DNA合成，软琼脂培养集落形成率减少及裸小鼠成瘤减慢。