In order to enhance the expression level of scu--PA in E. coli, studies have deen done on the alteration of 3'end of scu--PA cDNA gene and on the use of DnaY gene, which product can improve the translation of rare Arg condon in scu--PA cDNA gene.
为了获得人尿激酶原cDNA在E.coli中的高效表达，我们在其3`端改造，并在利用稀有Arg密码子即DnaY基因的识别方面展开了研究，表达产物由原来占菌体总蛋白的不到1％提高到15％。
Brinkmann U%Mattes RE%Buckel P High-level Expression of Recombinant Genes in Escherichia coli is Dependent on the Availability of the dnaY Gene Product 10.1016/0378-1119(89)90470-8 Gene, 1989, 1
隋广超%刘芳%胡美浩利用噬菌体T7RNA聚合酶在大肠杆菌中引导尿激酶原克隆基因的表达北京大学学报，1994，6
Coli. Thus a multicopy plasmid pUBS520 containing DnaY gene, which product can effec- tively translate AGA or AGG condons in scu--PA cDNA gene, was cotransformed into E. coli JA221 with pKK233--2/rscu--PA. By doing so, the expression level of scu--PA was enhanced by 5--fold.
通过多拷贝的相容性质粒pUBS520引进编码该tR-NA〓／AGG的基因DnaY，其产物可有效地识别AGA或AGG，因而尿激酶原的表达水平提高了5倍。
These findings suggested that HCY-2 gene with its product was dysmorphogenic to the neural tube, might be a genotoxic factor or teratogenic agent and should be responsible for chick NTDs and probably human NTDs as well.
结论是HCY-2基因对神经管闭合时期的胚胎神经系统发育具有明显基因毒性作用，其编码蛋白在神经管畸形发生过程中可能具有重要意义。
ORF identification involves sca ing each of the six reading frames and determining which one contai a stretch of DNA sequence bounded by a start and stop codon, yet containing no start or stop codo within it; a sequence meeting these conditio could corre ond to the actual single product of the gene.
ORF识别包括检测这六个阅读框架并决定哪一个包含以启动子和终止子为界限的DNA序列而其内部不包含启动子或密码子，符合这些条件的序列有可能对应一个真正的单一的基因产物。
It produces and displays the kinetic accumulation of PCR product----called amplification curve. Assuming that amplicons are amplified exponentially during the earlier cycles, we make use of the simple equation y=axto compare the start copy number of a gene in different samples, by measuring the threshold cycle. And with some standard, copy number of a gene can be
原理是利用能特异标记PCR产物的荧光物质，显示PCR 产物的动态累积，得到S 型的扩增曲线；假设该曲线的前期PCR 符合指数性扩增，于是在单纯指数方程的基础上，通过比较产物积累的速度来间接比较初始模板的分子数。
However where the product is nonproteinaceous, of low molecular weight and at the end of a long multienzyme sequence it is extremely unlikely that gene transfer to another host will be feasible.
然而，如果产品是低分子量且处于长多酶序列末端的非蛋白质类产品，转基因技术的应用可行性极小。
In general, identification was based on the worldwide-accepted 16-gene-loci system which including sexual genes. If probability of paternity is less than 99.99%, to different relations between half-siblings, siblings and mutation is a challenge. In addition to X or Y chromosomal and DNA STR typing and other newly adopted loci system, we also could make analysis of the two highly varied sequences of HV Ⅰ, HV Ⅱ which are on the D-loop of mitochondrial DNA, the maternal gene substances. We carried out PCR to amplify the mitochondrial DNA, HV Ⅰ and HV Ⅱ. After gel electrophoresis, we purify the PCR product. Then we use BigDye Terminator Sequencing Kit for sequencing, by use of DNA sequencing machine. We present three groups of mitochondrial DNA to study the application of mitochondrial DNA certification technique on identification.
一般身份鉴定首先以国际通用之包含性别的十六基因点位系统为基础分析，若有亲子关系概率不到99.99%或半手足、手足关系鉴定、疑突变等的情况下，为提高鉴别力，除依鉴定当时的需求增作X或Y染色体DNA STR式型别鉴定法和其他新增点位系统外，有时也须加做代表母系遗传之粒线体D-loop之两个高变异小区HV Ⅰ、HV Ⅱ碱基序列的分析，利用PCR技术分别复制粒线体DNA之HV Ⅰ、HV Ⅲ两段碱基，以琼脂电泳法确定PCR产物大小后纯化产物，以BigDye Terminator Sequencing Kit进行定序反应并纯化后以DNA定序仪分析。
Objective: To quantitative analyze the gene expression product--hGH, and determine expression time limit.
目的：定量分析基因表达产物人生长激素，并确定基因表达期限。
A new eucaryotic expression system of recombinant PoIFN-γ was also constructed so that the denaturalization and refolding process is avoided. The target gene coding PoIFN-γ2 mature protein was subcloned into expression vector, transformed into Pichia pastoris, The His〓 Mut〓 phenotype transformants were screened, fermented in flasks and induced by 1％methanol. The expressed product in culture solution and sedimentation have antiviral activity on VSV and immunological activity proved by indirected immunofluorescence antibody and Dot blotting assay.
为了构建可对表达产物修饰、加工的真核表达系统，使表达产物具有自然干扰素的活性，无需变性、复性，我们将PoIFN-γ2成熟蛋白基因连接酵母整合表达载体，电转化毕赤酵母，筛选了His〓Mut〓表型转化子，摇瓶培养，1％甲醇诱导表达，培养上清和菌体裂解上清均有抗VSV病毒活性，经间接免疫荧光抗体检测和Dot blotting鉴定，重组酵母菌诱导表达产物具有免疫活性。
The PCR product was purified by UNIQ-10 Column DNA Purification Kit to remove salts and primer-dimer. PCR product was cleaved by Msp I and HaeIII at the same time. The optimum separation condition in separating smaller than 70bp DNA fragments was applied in analyzing the mutation of codons 248 and 249 in p53 gene. The optimum separation condition was 8%LPA I with 8%mannitol, running pH at 6.5, running temperature at 15℃and electric field strength at 275V/cm. Mutation detection was completed in about 30min. Baseline separation and higher resolution (1.642) were obtained in separating 35bp and 40bp DNA fragments.
限制性内切酶MspⅠ和HaeⅢ同时切割纯化的PCR产物，然后以NGS-CE分离小于70bp DNA片段时获得最高分离度的电泳条件(筛分介质为8％LPAⅠ、添加剂甘露醇浓度为8％、pH为6.5、温度为15℃、电场强度为275V／cm)分析临床59例胃癌患者p53基因扩增产物的限制性片段长度多态性。30min内同时检测了p53基因中248位和249位两个密码子位点的突变情况，实现了35bp和40bp DNA片段的基线分离，且得到较高分离度(1.642)。
Objective Study on the effect of the expression product of human insulin gene in housefly.
目的 研究人胰岛素基因在转基因家蝇中所获得的表达产物对正常昆明小鼠血糖的影响。
For quantitatie real-time PCR of DR4 and DR5 transcripts, triplicated samples containing 9 l of cDNA with 10 l of Taqman Uniersal PCR Master Mix (Applied Biosystems, Foster City, CA) and 1 l of 20xAssays-on-demand Gene Expression Product (DR4; Hs 00269492_m1, DR5; Hs 00366272_m1, Applied Biosystems) were pre-incubated at 50oC for 2 minutes and subsequently at 95oC for 10 minutes.
对于进行定量实时PCR的DR4 和 DR5转录产物，使用10L 美国应用生物系统公司生产的 Taqman Uniersal PCR Master Mix试剂，1L的20xAssays-on-demand Gene Expression Product (美国应用生物系统公司生产的DR4； Hs 00269492_m1，DR5； Hs 00366272_m1)，先将其在50度下孵化2分钟，然后在95度下孵化10分钟，用这两种试剂将含有9L cDNA的样品扩增两倍。
The results indicated that positive results of Flt-3 expression were obtained in 48 out of 77 AL cell line, the positive rate was 62%; 12 cell lines were positive in 20 AML cell lines, the positive rate was 60%; 33 cell lines was positive in 57 ALL cell lines, the positive rate was 58%; 3 cell lines were positive in 5 CML cell lines, the positive rate was 60%. There was abnormal gene product in 1 AMOL cell line out of 12 AML cell lines with Flt-3 positive expression (positive rate 8.3%).
结果表明：77例AL细胞株中有48例Flt-3表达阳性，阳性表达率为62%，其中20例AML细胞株中有12例阳性，阳性表达率为60%；57例ALL细胞株中有33例阳性，阳性表达率为58%。5例CML细胞株中有3例阳性，阳性表达率为60%。12例Flt-3表达阳性的AML中有1例AMOL细胞株存在有异常表达（阳性率为8.3%），测序显示存在有29 bp的两个编码重复序列，其余Flt-3表达阳性的细胞株未检出重复序列。
The results indicated that positive results of Flt-3 expression were obtained in 48 out of 77 AL cell line, the positive rate was 62%; 12 cell lines were positive in 20 AML cell lines, the positive rate was 60%; 33 cell lines was positive in 57 ALL cell lines, the positive rate was 58%; 3 cell lines were positive in 5 CML cell lines, the positive rate was 60%. There was abnormal gene product in 1 AMOL cell line out of 12 AML cell lines with Flt-3 positive expression (positive rate 8.3%). DNA sequencing of abnormal gene product showed two coding duplication sequence with 29 bp long.
结果表明：77例AL细胞株中有48例Flt-3表达阳性，阳性表达率为62%，其中20例AML细胞株中有12例阳性，阳性表达率为60%；57例ALL细胞株中有33例阳性，阳性表达率为58%。5例CML细胞株中有3例阳性，阳性表达率为60%。12例Flt-3表达阳性的AML中有1例AMOL细胞株存在有异常表达（阳性率为8.3%），测序显示存在有29 bp的两个编码重复序列，其余Flt-3表达阳性的细胞株未检出重复序列。
There was abnormal gene product in 1 AMOL cell line out of 12 AML cell lines with Flt-3 positive expression （positive rate 8.3%）.
未分化B细胞系Flt-3基因表达阳性率明显高于成熟B细胞ALL（P＜0.05）。
Objective To understand the character of biochemistry and motabolism of Staphylococcus aureus, and relationship between entertoxin product and expression of dulcitol enzyme gene.
目的 了解金黄色葡萄球菌的生物化学和代谢特征，以及其半乳糖醇酶与产生肠毒素的关系。
Furthermore, androgen receptor and the ERG gene fusion product coordinately regulate components of the sarcosine pathway.
基因敲除甘氨酸N-甲基转移酶（此酶促进甘氨酸转化为肌氨酸）后，前列腺癌的侵袭性减弱。
In this study, we detected 56 patients among Chinese population of -α3.7 defect in alpha globin gene by PCR method, then the PCR product was digested by the restriction enzyme ApalⅠand BalⅠ.
本研究在中国人群中用PCR基因分析方法检出具有α珠蛋白基因-α3.7缺失的患者56例，然后用ApalⅠ和BalⅠ限制性内切酶进行分型。
The nucleocapsid protein gene of Akabane virus strain YN isolated from bovine was amplified by RT-PCR. The product of 696 bp fragment was obtained as expected. The amplified fragment was then cloned into the pDM18-T vector and confirmed by PCR, endonuclease analysis, and sequencing.
参考GeneBank发表的赤羽病病毒(Akabanevirus，AKAV)的核蛋白基因序列，设计合成一对引物，从分离自牛体的AKAVBHK21细胞培养物中提取总RNA，对AKAV核蛋白基因进行RT-PCR扩增，产物经琼脂糖电泳分析，呈现一条约696bp的条带，回收纯化后，将其克隆至pMD18-T质粒载体中，然后进行核苷酸序列分析。