In the BC2F1 and BC3F2 population, both grain length displayed two distinct phenotypic groups, the ratio of the two groups fit well to the expected ratio (1:1 and 3:1) of single locus Mendelian segregation, respectively, which indicated that the grain length in the BC2F1 and BC3F2 population were mainly controlled by a long-grain dominant gene.
利用SSR标记技术，通过集团分离法和隐性个体分析法，对BC3F2进行QTL定位，结果发现控制粒长QTL（暂定名GL2）位于第2染色体，与RM530连锁，遗传距离为4.1cM，是个尚未报道的粒长显性主效QTL。
Materials and methodsRandom abstraction have no consanguinity relation of 224 Liaoning Chinese male individual haematoporphyrin kind, use a normal regulations method to carry on abstraction, primer design and PCR to expand to the DNA, appliance ABI 3100-Avant gene analysis instrument, to the mini Y-STR locus DYS522, DYS508, DYS632 and DYS556 carries on an examination.
材料与方法随机提取无血缘关系的224例辽宁地区汉族男性个体血样，使用Promega的DNAIQ~系统提取DNA，引物设计和PCR扩增，应用ABI 3100-Avant基因分析仪，对DYS522，DYS508，DYS632和DYS556的mini Y-STR基因座进行检测。
The classical White locus is one of the important pig coat color hereditable loci, which is homologous to KIT gene.
通过利用PCR-RFLP和PCR-SSCP技术对中国地方猪种KIT基因内含子17、18的序列进行多态性分析。
By genotyping in 127 cases using PCR-SSCP analysis, we have gotten donors who are compatible with the recipient for one locus of HLA class Ⅱ gene.
对配型一致及不一致的供体和受体，进行体外混合淋巴细胞培养，以检验这种基因配型方法的可靠性。
In the present research, the polymorphism of A746G of bone morphogenetic protein receptor IB, FecXG and FecXL locus of bone morphogenetic protein 15 (BMP15), exon 1 and exon 2 of growth differentiation factor 9 (GDF9), intron 9 and exon 10 of prolactin receptor, exon 1 of estrogen receptor and the 5` flanking region of insulin-like growth factor-I gene in 330 sheep of eight breeds or strains, which including Small Tail Han, Poll Dorset, Suffolk Germany Mutton Merino and Chinese Merino including Prolific strain (PS, Meat strain and Large Frame strain, were detected and the relationship between genotypes and average litter size were analyzed. The results showed that (1) the A746G mutation in BMPR-IB gene was found not only in STH but also in PS, MS and S.
本研究采用候选基因法对BMPR-IB基因的A746G突变、BMP15基因的FecXG和FecXL位点、GDF9基因的外显子1和外显子2、PRLR基因的内含子9和外显子10、ESR基因的外显子1以及IGF-I基因的5`-侧翼区在小尾寒羊、中国美利奴多胎品系、中国美利奴肉用品系、中国美利奴体大品系、中国美利奴、德国肉用美利奴、萨福克以及陶赛特8个品种中约330例的多态性及其与平均窝产羔数间的关系进行了研究。
Here we report the cloning and sequencing of pig ob cDNA gene (Genbank Accession No. AF052691), and the polymorphic analysing on ob locus in swine.
目前，关于畜禽肥胖基因的研究报道还非常少，国内外还没有全长畜禽 ob基因序列的报道，本研究克隆分析了猪肥胖基因的全长cDNA序列(Genbank Accession Number：AF052691)，并对不同品种猪在该位点的多态进行了分析。
Furthermore, among the cases of AML showed LOH, 71.4%(5/7) demonstrated LOH at multi-locus. None of 11 cases of CL and 7 cases of MDS showed LOH at these 4 loci. MSI was observed only in 3 cases of leukemia (3/51) and 1 case of MDS. AML showed significantly higher LOH frequencies than ALL on 8p22, which suggest that there may be some gene alterations related to AML.
AML在D8S559和D8S555位点LOH发生率(22.7％和31.8％)显著高于ALL(0％和5.6％)(四格表精确检验法，P=0.040和P=0.044，p＜0.05)，并且在发生LOH的AML病例中，71.4％(5／7)表现为多位点的LOH.11例CL和7例MDS患者所选4个位点均未检测到LOH.51例白血病患者仅有3例出现所选位点的MSI，7例山血病忠合SLJ染色休撤卫星不稳定性和杂和性缺失的研究 MDS患者中，仅有1例发生了MSI。
M ETHO D S: Serum IL26 levelw asm easured by EL ISA, and thedistribution of IL26 gene locus polym orphism at 2572 and 2643sitesw as analyzed by PCR 2R FLP m ethods in 126 CHD patientsand 108 age2m atched norm alcontrols.
采用聚合酶链反应 2限制性片段长度多态性技术对 126例老年冠心病中患者及 108例正常人 IL26 2572和 2643位点基因多态性进行研究，同时检测血清 IL26水平。
From multiple sequence alignment and phylogenetic analysis of storage protein gene family, it is concluded that the subunits Dy10.5t、Dy10.4t and Dy10.5*t of Ae. tauschii are similar to the Glu-1 locus found in wheat and related species, and are new members of y-type HWM glutenin family.
通过对这3种HMW-GS体外表达及与32个已知氨基酸序列的HMW-GS多序列比对和系统进化关系分析，证实Dy10.5t、Dy10.4t和Dy10.5*t 3个亚基是D基因组编码的高分子量谷蛋白y-型亚基家族的新成员，同时为研究小麦族的起源和进化提供有用的信息和证据。
Results The chromosome-plasmid balanced lethal gene expression system of Vibrio cholerae based on thyA locus was constructed.
进而构建了以thyA基因为选择压力的霍乱弧菌染色体-质粒致死平衡系统。
He ascribes the condition to a single-locus recessive gene for dwarfish stature without affecting endocrine organs and function.
他将这种病况归因於像侏儒身高的隐性基因缺乏影响内分泌的器官及功能。
Based on the structure of gene frequency matrix, we displaye the different patterns of Scallergram of correspondent analysis from different types of loci (HLA-A locus, and STR- CSF1PO locus in Chinese Han populations).
从分析基因频率矩阵的结构特点入手，以实例验证和比较了对应分析中散点图的结构特征。
There was a mutation from A to C on mutation locus of GH4 gene in the 64bp.
GH基因第3外显子对体高、胸围、管围无显著性影响(P＞0.05)。
To observe the effect of Sire2, a Down syndrome critical locus gene, on the differentiation of PC12 cells, and to explore the role of Sim2 in the pathogenesis of Down syndrome, PC12 cells were transfected with the pcDNA3-mSim2 ptasmid.
以pcDNA3-mSim2真核表达载体稳定转染PC12细胞，探讨位于Down综合征关键位点的Sim2基因对PCI2细胞分化的影响及其机制。
The result showed that there are three genptypes in the third mutation locus of Prepro-orexin gene. including
结果表明，十个猪群Prepro-orexin基因在第62bp位点处存在变异，表现为三种基因型T／T、T／C、C／C。
A CA repeat polymorphic sequence in D5 S346 locus 40 Kb downstream from the APC gene, which located at chromosome 5q21-q22, was examined in 30 sporadic colorectal carcinomas.
应用APC基因下游40Kb处的微卫星多态标记D5S346，检测30例大肠癌组织DNA，在可提供信息的15例杂合子中检出5例5q21＿q22的杂合性丢失，占33％。
The EHEC virulence plasmid containing a complex antibiotic-resistance gene locus, designated as pO26-CRL, was purified from EHEC O26:H (patient with hemorrhagic colitis) and subjected to shotgun-sequencing and bioinformatic analysis. Determination of the 111, 481-bp sequence of pO26-CRL revealed genes encoding a functional enterohemolysin operon, STEC-specific extracellular serine protease, putative EHEC adhesin, catalase/peroxidase, and myristoyl transferase involved in lipid A synthesis.
自EHEC O26：H纯化出包含有抗生素耐药基因座复合物也就是pO26-CRL的EHEC毒性质粒，并对该质粒进行鸟枪法测序和生物信息学分析。pO26-CRL序列111，481-bp的测定揭示了编码一个功能性肠溶血素操纵子、细胞外STEC特异性丝氨酸蛋白酶、假定的EHEC粘附素、过氧化氢酶/过氧化物酶以及与脂质A合成有关的十四酰转移酶的基因序列。
Methods The rabies glycoprotein gene was introduced into the TK locus of Chinese Tiantan strain of vaccinia virus, and a fragment between fragment C and fragment K involving vaccinia virus virulence and host-range related genes were deleted by homologous recombinant.
将编码中国狂犬病毒5 aG株糖蛋白的基因，插入痘苗病毒天坛株的TK区，获得重组病毒VTKRG，通过两步同源重组，删除CK片段间与痘苗病毒毒力及宿主范围相关的基因，得到非复制型重组痘苗病毒VTKRG△CK。
For carcass traits, significant effects were observed for this locus on BFT1 and BFT2; For meat quality traits, this locus was near to significantly associated with pH and pH and seemed to be dominant in action.(5) LMCD1 gene was highly expressed in heart and muscle while it had a broad expression spectrum across all tissues examined.3、NRAP (Nebulin-related anchoring protein):(1) The full coding sequence of LargeWhite, Landrace and Meishan breeds were isolated. The nucleotide sequence of 5'RACE (rapid amplification of cDNA end) and intron 39 and 40 have been deposited to GenBank under accession number DQ157553 and DQ480149.(2) 35 SNPs were identified by comparing sequences, three of which resulted in amino acid change.(3) PCR-Eco721-RFLP was developed to detect A/G substitution in intron 39. In unrelated pigs from eight breeds, both allele A and B were found in Chinese pig breeds, while only B was in LargeWhite and Landrace.(4) Association analysis in 302 F_2 animals showed significant associations between Eco721-RFLP genotypes with BWT, CFW, LEH and BFT1 for carcass traits and pH, pH, MCV1, MCV2, DLR and WHC for meat quality traits. This locus was predominant in dominance for carcass trait, but in addition for meat quality.(5) NRAP mRNA was transcribed especially in heart and skeleton muscle.4、ECH1 (Enoyl CoA hydratase 1):(1) The full coding sequence of LargeWhite, Landrace and Meishan breeds, 5'RACE and all introns except intron 3 were isolated and deposited to GenBank under accession number DQ157552 and DQ480146DQ157552.(2) 17 SNPs were identified, of which one was cSNP.(3) Two A/G substitution were detected by PCR-BamHⅠ-RFLP in intron 1 and PCR- PstⅠ-RFLP in intron 5 separately, and T/C mutation in exon 4 was detected by PCR-SSCP. Among seven different pig breeds examined, results of BamHⅠ-RFLP in intron 1 showed that the frequency of allele B was near to 1 in LargeWhite and Landrace; both allele A and B were found in Chinese pig breeds, and allele B was predominant in other breeds except Meishan and Qingping. For PCR- PstⅠ-RFLP in intron 5, in seven commercial pig breeds, both alleles C and D were detected in all other examined breeds except Jianli pig which only had allele D.(4) Association analysis in 296 F_2 animals showed significant associations between BamHⅠ-RFLP genotypes and CFW, LEH and WM. And this locus seemed to be dominant in action. For PCR- PstⅠ-RFLP in intron 5 in 306 F_2 animals, for carcass traits, significant effects were observed for this locus on DP and AST; For meat quality traits, this locus was significantly associated with MCV2.(5) Expression of ECH1 gene can be detected in all tissues examined: heart, liver, lung, kidney, spleen, stomach, small intestine, muscle and fat.5、CKMT2 (creatine kinase, mitochondrial 2):(1) The full coding sequence of LargeWhite, Landrace and Meishan breeds were isolated and deposited GenBank under accession number DQ363337. In addition, the sequence of intron 4 can be seen in appendix 14.(2) Of nine SNPs identified within these sequences, three of which resulted in amino acid change.(3) An PCR- MspⅠ-RFLP was developed to detect the A/G polymorphism in intron 4. Among nine pig breeds examined, allele B was 1 or near to 1 in Meishan and Erhualian, while in other seven breeds all were 1 of allele A.(4) 302 F_2 animals were typed for association analysis. For carcass traits, significant effects were observed for this locus on BWT, IFR, FMP, CL, BFT1, BFT2 and BFT3, and their additive effects were predominant; For meat quality traits, this locus was significantly associated with WM.(5) Expression of CKMT2 gene was predominant in heart and skeleton muscle and moderate in lung, kidney and fat, although a weak signal was observed for liver, ovary and testis, and no expression in spleen, stomach, small intestine and uterus.6、TTID (Titin Immunoglobulin Domain Protein):(1) The full coding sequence of LargeWhite, Landrace and Meishan breeds and sequences of all introns except intron 2 and 7 were isolated and deposited to GenBank under accession number DQ157551 and DQ480148.(2) Total 51 SNPs within these intron sequences and no mutation or deletion was found in cDNA sequences.(3) An PCR- HinfⅠ-RFLP was developed to detect T/C substitution in intron 6. Among ten pig breeds which were typed by HinfⅠ-RFLP, results showed that there were significantly different in allele frequencies between Chinese indigenous pig breeds and foreign pig breeds: the frequencies were 1 or near to1 in LargeWhite and Landrace, while allele A was predominant in other five breeds although allele B were only a bit dominant in Erxihei, Qinsping and Bamei.(4) 279 F_2 animals were typed for association analysis. For carcass Waits, significant effects were observed for this locus on SP, LEA and AST, and their additive effects were significant; For meat quality traits, this locus was significantly associated with pH, MCV1 and WM, and their additive effects were significant.(5) Expression of TTID gene was highest in skeleton muscle and heart, with weak level in fat and lung, and no signal in other tissues.7、PFKM (Phosphofructokinase Muscle Type):(1) The full cDNA sequence of LargeWhite, Landrace and Meishan breeds and sequences of 2-21 introns were isolated and deposited to GenBank under accession number DQ363336 and DQ480147. And the length of sequence of intron 1 was identified.(2) Of the 111 SNPs identified within all sequences, four of which resulted in amino acid change.(3) PCR-TaqⅠ-RFLP were developed to detect T/C substitution in exon 13 and exon 17. In unrelated individuals from seven different pig breeds, allele frequencies of the TaqⅠ-RFLP in exon 13 was compared: frequencies of allele B in LargeWhite, Landrace, Erxihei were 1 or near to 1, and a little dominant of allele B in other four Chinese pig breeds. For the same polymorphism in exon 17, the trend of allele frequencies distribution was the similar to that of exon 13.(4) For association analysis in the 214 F_2 animals, significant effects were observed for different genetype of exon 13 on BWT, IFR, FMP, LMP, RLF, LEH, BFT1, BFI2, BFT4 and ABF of carcass traits. It is notable that both additive effects and dominant effects in BWT, LMP, RLF and LEH were very significant; For meat quality traits, this locus was significantly associated with MCV2, MM1, WM and IMF. Association analysis in 241 F2 animals showed there were significant effects on BFI3 and ABF of carcass traits and on MM1, MM2, DLR, WHC, IMF and WM of meat quality traits with TaqⅠ-RFLP in exon 17.(5) Expression of PFKM gene had different levels in each examined tissue except stomach and small intestine, with highest in skeleton muscle, then in heart.8、MYLPF (myosin light chain, phosphorylatable, fast skeletal muscle):(1) The full genomic sequence of of LargeWhite, Landrace and Meishan breeds were isolated and deposited to GenBank under accession number DQ533994.(2)43 SNPs, including 5 cSNPs, were found by comparing sequences of three pig breeds, three of which resulted in amino acid change.(3) PCR-TaqⅠ-RFLP and PCR-MspⅠ-RFLP were developed to detect G/A substitution and T/C substitution in intron 1 separately. For MspⅠ-RFLP among seven different pig breeds examined, the frequency of allele B was two times of that of allele A in Landrace, while allele B were predominant in other breeds. For TaqⅠ-RFLP, the frequency of allele B was two times of that of allele A in LargeWhite and Landrace.(4) The locus of MspⅠ-RFLP was typed in 140 pigs (Landrace, LargeWhite, Meishan, LargeWhite×Landrace and Landrace×Large White pigs) to study the association between carders of different genotype and the trait values. For carcass traits, significant effects were observed for this locus on LMP, SP, LEW and LEA, with approximative significant on BFT1 and LEH(P＜0.06). This locus appeared to be additive effects with different action direction; For meat quality traits, this locus was significantly associated with MM1, MM2 and WM and IMF, which appeared to be predominant on addictive effects.(5) RT-PCR analysis of MYLPF showed its expression in all tissues examined, with highest levels in heart and skeleton muscle.
在所检测的6个猪种中，大白、长白、鄂西黑和皖南花猪种G等位基因的基因频率为1或接近1，而梅山和清平品种的等位基因G基因频率分别为0.2889和0；(4)在178头大×梅F_2代资源家系中进行标记性状关联分析，该位点与胸腰椎间背膘厚和臀部平均背膘厚差异显著，该位点以显性作用方式为主，且方向一致；(5)该基因在所检测的各组织中均有表达，在心脏和肌肉中表达量最高。3、NRAP(Nebulin-related anchoring protein，伴肌动蛋白相关锚定蛋白)：(1)获得了大白猪、杜洛克猪、梅山猪三个猪种基因编码序列、5`RACE(rapid amplificationof cDNA end，快速扩增cDNA末端)及其第39、40内含子序列，序列已提交到GenBank中，登录号为DQ157553和DQ480149；(2)发现了35个SNPs，其中3处预测可导致氨基酸改变；(3)建立了第39内含子A／G替换的PCR-Eco721-RFLP分型方法；在所检测的8个猪种中，大白、长白猪种B等位基因的基因频率为1，中国地方猪群可检测到A和B等位基因；(4)在302头大×梅F_2代资源家系中进行标记性状关联分析，该位点与出生重、内脂率、眼肌高度、肩部背膘厚、背最长肌pH值、失水力、系水力、背最长肌肌肉色值、股二头肌pH值和股二头肌色值显著或极显著相关，该位点在胴体性状以显性作用方式为主，肉质性状则以加性作用为主；(5)该基因特异性的在心脏和骨骼肌中表达。4、ECHI (Enoyl CoA hydramse 1，烯酯酰辅酶A水合酶1)：(1)获得了大白猪、长白猪、梅山猪三个猪种基因编码序列、5`RACE及第3内含子外的所有内含子序列，并提交到序列的GenBank登录号为DQ157552和DQ480146；(2)序列比对发现了17个SNPs，其中1处cSNP；(3)建立了第1内含子A／G替换的PCR-BamHⅠ-RFLP、第5内含子A／G替换的PCR-PstⅠ-RFLP分型方法和第4外显子T／C突变的PCR-SSCP分型方法；第1内含子的酶切多态，在所检测的7个猪种中，大白和长白猪种B等位基因的基因频率接近为1，而中国猪种均可检测到A和B等位基因，除清平猪种外，其余品种均为B等位基因占优势；关于第5内含子多态性分布，在所检测的7个猪种中，监利猪未检测到A等位基因，其余猪种均可检测到A和B等位基因，均为B等位基因占优势；(4)在296头大×梅F_2代资源家系中进行标记性状关联分析，第1内含子多态的不同基因型间内脂率、眼肌高度和肌内水分等生产性状差异显著，以显性效应为主；在306头大×梅F_2代资源家系中进行标记性状关联分析，第5内含子位点与屠宰率、平均皮厚和股二头肌色值差异显著；(5)该基因在所检测的9个组织中均表达。5、CKMT2 crcatine kinase，mitochondrial 2(sarcomeric，肌节线粒体肌酸激酶2：(1)获得了大白猪、长白猪、梅山猪三个猪种基因cDNA序列，该基因GenBank登录号为DQ363337，获得的第4内含子序列见附录14；(2)序列比对，发现了9个SNPs，其中3处预测可导致氨基酸改变；(3)建立了第4内含子A／G替换的PCR-MspⅠ-RFLP分型方法，在检测的9个猪种中，梅山和二花脸猪中B等位基因频率为1或接近1，其他7个猪种均为A等位基因频率为1；(4)在302头大×梅F_2代资源家系中进行标记性状关联分析，该位点与出生重、内脂率、肥肉率、胴体长、肩部背膘厚、胸腰椎间背膘厚、臀部背膘厚和肌内水分等性状差异显著，以加性作用方式为主；(5)该基因在心脏和骨骼肌中表达丰度最高，在脾脏、胃、小肠和子宫中不表达，在其余组织不同程度的表达。6、TTID (Titin Immunoglobul in Domain Protein；肌联蛋白免疫球蛋白区域蛋白)：(1)获得了大白猪、长白猪、梅山猪三个猪种基因全长cDNA序列以及除第2、7内含子外的所有内含子序列，提交到GenBank的登录号为DQ157551和DQ480148；(2)序列比对发现了51个SNPs，该基因cDNA中没有发现碱基突变或缺失；(3)建立了第6内含子T／C替换的PCR-HinfⅠ-RFLP分型方法，在检测的10个猪种中，在大白和长白猪种中B等位基因的频率为1或是接近1，国内品种中除鄂西黑猪、清平猪和八眉猪中B等位基因频率略占优势外，其余5个品种均为A等位基因占绝对优势；(4)在279头大×梅F_2代资源家系中进行标记性状关联分析，胴体性状方面，该位点与皮率和眼肌面积差异显著，与平均皮厚差异极显著；并且表现为加性效应显著或极显著，肉质方面，该位点与股二头肌pH值、背最长肌和肌内水分差异显著，表现为显性效应显著；(5)该基因在心脏和骨骼肌中表达量很高，在脂肪和肺中微量表达，其余组织中不表达。7、PFKM (Phosphofructokinase Muscle Type，肌型磷酸果糖激酶)：(1)获得了大白猪、长白猪、梅山猪三个猪种基因全长cDNA序列及第2到21内含子序列，验证猪中第1内含子的大小，序列GenBank的登录号为DQ363336和DQ480147；(2)序列比对发现了111处SNPs，其中4处预测可导致氨基酸改变；(3)建立了第13外显子T／C替换和第17外显子的PCR-TaqⅠ-RFLP分型方法，在所检测的7个猪种中，第13外显子多态性分布频率在检测的大白、长白和鄂西黑猪中B等位基因频率为1，另外四个国内猪种均为B等位基因占优势，在所检测的7个猪种中，第17外显子多态性分布频率基本同第13外显子位点的分布，(4)在214头大×梅F_2代资源家系中进行标记性状关联分析，第13外显子位点基因型不同时，出生重、内脂率、肥肉率、瘦肉率、瘦肥比率、眼肌高度、6—7胸腰椎间背膘厚、胸腰椎间背膘厚、肩部背膘厚、三点平均背膘厚、股二头肌色值、背最长肌大理石纹、肌内水分和肌内脂肪差异显著或极显著；该位点在部分胴体性状加性效应和显性效应均为极显著；在241头大×梅F_2代资源家系中进行标记性状关联分析，第17外显子位点与眼肌高度、臀部背膘厚、三点平均背膘厚、大理石纹、失水率、系水力、肌内脂肪和肌内水分差异显著或极显著；(5)该基因在心脏和骨骼肌中表达量很高，胃和小肠没有表达，其他组织均有不同程度的表达。8、MYLPF (myosin light chain，phosphorylatable，fast skeletal muscle，肌浆球蛋白轻链可磷酸化蛋白)：(1)获得了大白猪、长白猪和梅山猪的基因组序列；序列GenBank的登录号为DQ533994；(2)序列比对，发现了43处SNPs，有5处cSNPs，其中3处预测可导致氨基酸改变；(3)建立了第1内含子G／A替换的PCR-TaqⅠ-RFLP和TIC替换的PCR-MspⅠ-RFLP分型方法；在所检测的7个猪种中，第1内含子MspⅠ-RFLP多态性分布频率在长白猪中A等位基因与B等位基因频率的比例大约为1：2，其余猪种为B等位基因占绝对优势；在所检测的7个猪种中，第1内含子TaqⅠ-RFLP多态性分布频率，在大白和长白猪中A等位基因与B等位基因频率的比例大约为1：2，国内猪种均为B等位基因占绝对优势；(4)在140头试验猪群(长白、梅山、长白、长白×大白、大白×长白)中进行标记性状关联分析，基因型不同时，瘦肉率、皮率、股二头肌大理石纹、背最长肌大理石纹和肌内水分差异显著，眼肌宽度、眼肌面积和肌内脂肪差异极显著，肩部背膘厚和眼肌高度差异接近显著水平；该位点在这些性状上均表现为加性作用方式，但作用方向不同；(5)该基因在各组织中均有表达，尤以心脏和骨骼肌中表达丰度最高。
To investigate the influence of genetic variation at the CRP gene on CRP levels, we first constructed a dense linkage disequilibrium map for common single-nucleotide polymorphisms spanning the CRP locus (1 SNP every 850 bases, 26 kilobase genomic region).
为了调查基因指标对C－反应蛋白基因变量的影响，我们先为单核苷酸多态性组合了一种密集的连续平衡不稳定图谱，以此来生成CRP的基因定位（每850B，26KB基因组区域形成一个单核苷酸多态性）。