The electrochemical and spectrometric studies on the interactions of two organic dye molecules, malachite green and pyronine B, with DNA have been described in this paper.
本论文以电化学和紫外-可见光谱方法研究了两种有机染料小分子孔雀石绿和吡哕红B与dsDNA的相互作用。
The system termed Red consists of three proteins:Exo protein, which binds to a dsDNA end and processively degrades linear dsDNA in a 5'to 3'direction; Beta protein, which binds to ssDNA and promotes strand annealing; and Gam protein, which binds to the bacterial RecBCD enzyme and inhibits its activities.
Red重组系统由三种蛋白组成：Exo蛋白是一种核酸外切酶，结合在双链DNA的末端，从 5'端向 3'端降解DNA ，产生 3'突出端；Beta蛋白结合在单链DNA上，介导互补单链DNA退火；Gam蛋白可与RecBCD酶结合，抑制其降解外源DNA的活性。
The effects of the concentration of methyl cellulose solution, running pH, running temperature and electric field strength on dsDNA (＜600 bp) analysis were investigated in the experiment. The optimum separation condition was obtained and applied to mutation detection of H-ras gene at codon 12 of 59 gastric cancer tissues.
以甲基纤维素(Methyl cellulose，MC)为筛分介质，用pUC19 DNA/Msp I Marker标准DNA片段为实验对象，通过考察筛分介质的浓度、pH值、毛细管的温度和运行电压优化出分离小于600 bp的双链DNA片段的最适条件，并将此方法应用于临床59例胃癌患者肿瘤组织H-ras基因12位密码子点突变情况的检测。
Results The scnsitivity and specificity of anti-C1 q and anti-dsDNA Abs in lupus active patients were 63.88% and 69.44%, 81.82% and 86.36% respectively.
狼疮性肾炎组dsDNA水平显著高于非狼疮性肾炎组，尿蛋白≥患者C1q抗体水平显著增高。
UV-Vis spectra, DNA viscosity titration, competition experiment and the effect of dsDNA and ssDNA on the fluorescense intensity were used to identify the binding mode.
结果 DNA 对巴罗沙星的荧光猝灭常数为(5.43±0.07)×10~3L·mol~(-1)，ΔH=-8.03 kJ·mol~(-1)；Mg~(2+)使巴罗沙星与 DNA 的作用增强。
It is an enveloped, non-occluded, bacilliform and circular dsDNA virus.
是一种具有囊膜的、无包涵体的、类杆状双链环状DNA病毒。
The interactions of dsDNA and ssDNA with two osmium complexes have been studied by surface electrochemical methods based on DNA modified electrode. It is found that both osmium complexes bond DNA with electrostatic interaction. And some thermodynamics and kinetics constants, such as bindind constant, ratio of binding constants K〓/K〓, limiting ratio K〓°/K〓°at zero ionic strength (μ＝0), free energy ΔG〓, dissociation rate constant k and binding site size s have been obtained.
研究发现两种锇配合物都是通过静电作用与DNA结合，并计算得到联吡啶锇和二氯菲咯啉锇与dsDNA和ssDNA相互作用的结合常数、结合常数比K〓／K〓、μ＝0时极限结合常数比K〓°／K〓°、结合自由能ΔG〓、解离速度常数k和结合位点数s等多个热力学和动力学参数。
By optical tweezers manipulation, we can study the relationship between the applied force and the extension of dsDNA to understand the unwinding process. In this thesis, I have studied the force-extension relation of dsDNA in lower force regime (0~10 pN).
在我们的实验中，藉由操控雷射单光钳，我们可以量测在低外力的作用下(0~10 pN)双股DNA的伸长量，进而了解双股DNA的弹力行为。
An icosahedral shell consisting of 415 copies of capsid protein encloses a 39.7 kilobase dsDNA genome.
一个廿面体病毒壳体由415个病毒衣壳蛋白质拷贝组成，包围着一个39.7千个碱基对双股脱氧核糖核酸基因组。
In order to investigate the potential role of human ERMAP gene in erythropoiesis, the ERMAP-dsDNA was designed, ERMAP-shRNA expressing plasmids was constructed, and ERMAP-shRNA/K562 cell was established. Cell morphology, biphenylamine staining, expression of cell surface antigens as well as quantitative level of human ER-MAP gene were observed during K562 cells differentiating toward erythroid lineage induced by Ara-C.
为探讨人类ERMAP基因在红细胞分化发育过程中的作用，本研究设计ERMAP-dsDNA，制备ERMAP-shRNA表达质粒，并建立稳定表达ERMAP-shRNA的K562细胞系（即ERMAP-shRNA/K562细胞系），观察Ara-C诱导ERMAP-shRNA/K562细胞向红细胞系分化过程中，细胞形态、联苯胺染色细胞阳性率和细胞表面标记等的变化，同时以FQ-PCR检测K562细胞人类ERMAP基因表达量的变化。
Objective To discuss the significance of anti-nuclear antibodies, Anti-brine extracted antigen peptide antibody spectrum, Anti-dsDNA, Anti-neutrophil cytoplasmic antibodies, Anti cardiolipin antibodies, in diagnosis, monitoring and treatment of lupus nephritis.
作者：钟路符克英陈汝黄白丽阮和球庞海云作者单位：海南省人民医院，海南海口 570311。目的探讨抗核抗体、抗可提取性抗原多肽抗体谱、抗双链DNA抗体、抗中性粒细胞胞浆抗体和抗心磷脂抗体在狼疮性肾炎诊断及治疗中的意义。
Direct single-photon photo-ionization of Visnagin and Khelin under 308 nm in buffered aqueous solution results in formation of 〓 and 〓, which can effectively oxidize dGMP, ssDNA and dsDNA.
对潜在的光敏剂Visnagin和Kellin在308nm激光激发下发生的单光子电离过程进行了研究，发现电离产生的阳离子自由基具有很强的氧化性，能够快速氧化dGMP、ssDNA和dsDNA产生鸟嘌呤阳离子自由基。
The frequency of DMB*0103 tended to increase in anti-dsDNA group of SLE(Pc.001) and OR was 6.49.5 environmental risk factors (damp, mind stimulation, sunlight, sword bean, infection of tonsil) were selected to enterthe logistic model.
DMB*0101在抗-dsDNA组的频率明显高于抗-dsDNA组(P＜0.05)，但校正后差异无显著性；DMB*0103在抗-dsDNA组的频率明显高于抗-dsDNA组(Pc＜0.0001)，其OR值为6.49(其OR的95％CI为2.85-14.98)。logistic回归分析显示的SLE环境危险因素有5个(潮湿、精神刺激、日晒、刀豆、扁桃体发炎)，女性个人婚育史中的出生母亲年龄、月经初潮年龄、自然流产次数与SLE的发病有关。
Moreover, in some SLE patients serum level of BAFF is elevated and correlated with serum anti-dsDNA titer.
部分系统性红斑狼疮患者中BAFF的水平有不同程度增高，并与抗dsDNA抗体滴度相关。
Additionally, the grafted PDMA has excellent dynamic coating ability which is important to stabilize and suppress the EOF during the separation.
在对Φ×174/HaeIII digest样品进行毛细管电泳分离实验中，详细研究了聚合物溶液浓度、聚合物接枝密度和分离电压对dsDNA分离性能的影响。
Conclusions The IgG subtype of anti-dsDNA antibodies may bind to MCs by cross-reactive antigen of α - actinin.
结论IgG型抗dsDNA抗体可能通过α-辅肌动蛋白与系膜细胞发生交叉结合反应。
α - actinin is a cross-reactive antigen for the binding of IgG subtype of anti-dsDNA antibodies to mesangial cells
α-辅肌动蛋白在IgG型抗双链DNA抗体与系膜细胞结合中的作用
Results Anti dsDNA antibodies and other antinuclear antibodies were induced by lymphocytes activated by Con A/LPS or IL 2 or by active nuclei and chromatin. Anti DNA antibody positive mice had marked immune complex deposits in glomeruli.
结果上述原因活化的淋巴细胞及其活性染色质均能诱导抗双链DNA抗体和其它抗核抗体生成，免疫小鼠的肾脏有显著的免疫复合物沉着。
To determine the following disease activity indexes before and after treatment: antinuclear antibodies, anti-dsDNA autoantibodies, complement C3, erythrocyte sedimentation rate, routine urine examination, 24-hour proteinuria estimation and kidney function.
治疗前和治疗6个月后检测疾病活动指标抗核抗体、抗双链DNA抗体、补体C3、血沉、尿常规、24h尿蛋白定量、肾功；
Study on the interaction between azure and dsDNA from calf thymus
小牛胸腺DNA与天青I相互作用研究