After rearing on healthy aphids (Rhopalosiphum pseudobrassicae Davis) for 3-4 days, DNA samples were extracted from the surface of ladybeetle bodies treated or untreated with alkaline solution and Dnase. PCR products specific for HaSNPV-AaIT were found in several samples from the untreated ladybeetle bodies, whereas no such products were found from the bodies after the alkaline and Dnase-treatment by both PCR and dot blot hybridization.
另外，从多次施用过重组病毒的棉花田中采集了120只龟纹瓢虫和七星瓢虫，利用健康蚜虫饲养34d，用碱解液处理瓢虫体表后，从处理液中可以检测到病毒DNA；但瓢虫体表经碱解液和Dnase处理后，从瓢虫体内提取的基因组DNA，用PCR和斑点杂交的方法，都没有检测到AaIT的序列存在。
The modified CTAB can isolate high quality RNA from wild jujube seedlings. After exorcizing DNA from RNA by DNase I, the cDNA fragment synthesized with M-MLV reverse transcriptase was 100bp to 1500bp, satisfied to the requirement of cDNA-PCR.
采用改良CTAB法提取了酸枣幼苗总RNA，用DNaseⅠ去除RNA中的DNA后，选用M-MLV反转录酶合成了cDNA第一链，片段大小在100～1500bp之间，可以满足下一步cDNA分析试验的要求。
To characterize the effects of bivalent metal ions on the activation of enzymatic reaction of the T2A-DNase domain, we found that the T2A-DNase domain can adopt various bivalent metal ions for incorporation into its metal binding site as well as for the activation of the DNase activity.
分析ColE7 T2A-DNase domain-Im7复合体的晶体结构，我们发现ColE7的T2A-DNase domain中含有一个锌离子，因此测试1 pM~100 mM范围浓度的八种二价金属离子对DNase活性的影响以了解二价金属离子所扮演的角色。
In this study, PCR-based genomic fingerprinting technique has been employed to identify genomic DNA fragments containing DNase I-hypersensitive sites and differentiation-dependent regulatory sequences.
在本实验中，利用PCR指纹技术来寻找基因体中DNase I高敏感度的DNA片段及与细胞分化相关的调控序列。
Due to limited genomic information to date, we have focused attention on four DNase I-hypersensitive clones that are associated with known transcription units, including ODC, PACE4, C2, and cathepsin precursor.
利用PCR及南方墨点法已经确定其中四个片段为DNase I高敏感性DNA片段，接著分析这四个片段是否带有与分化相关的调控序列。
Results Chromatin DNA of hypothalamus and pituitary gland in old rats existed a repeat length (nucleosome core and linker region) of (190±7)bp and (171±8)bp, and in young rats (193±9)bp and (170±5)bp respectively.
结果 老年大鼠下丘脑、垂体染色质核小体重复DNA长度分别为（190±7）bp和（171±8）bp，而青年大鼠分别为（193±9）bp和（170±5）bp；DNase I酶解DNA产物PAGE显示各年龄组染色质DNA均存在10 bp间隔重复结构和相同的电泳区带分布特征；DNase I染色质降解率老年大鼠明显低于青年大鼠。
Recent studies on the active gene dist-ributions on the mitotic chromosomes byKerem, Adolph and Gazit have demonstratedthat the mammalian chromosomes on the con-ventionally prepared slides can be nick-translated in situ in the presence of DNase-1, DNA polymerase and nucleotide trisphosphatesby using the〓H-dTTP or biotinylated dUTP asa label.
最近，Kerem，Adolph和Gazit等通过研究有丝分裂中期细胞染色体上的活性基因分布，〓明在DNase—1，DNA聚合酶及三磷酸核苷酸存在的反应体系中，可用氚—dTTP或生物素化的dUTP作标记物，对载玻片上的哺乳动物染色体进行原位切口移位，以研究染色体上的DNase—1敏感区。
Based on twin primer design, we developed a general real-time method to trace nuclease activity.
在孪生引物的基础上设计出荧光淬火双链探针，建立了研究DNase Ⅰ酶切活性的实时示踪方法。
The toxicity of colicin is inhibited by binding with its inhibitor, Im protein. Thus this is a good model system for the investigation of protein-protein recognition and interaction.
大肠杆菌素是一种非特异性的DNase，一旦受到环境压力时，会由菌体中释放出来穿透敏感细菌的细胞膜而引起死亡。
We speculate that in addition to the cytotoxic effect, colicin seems likely to exert a novel effect of protecting the producing cells against bacteriophage infection.
最后以活体外及活体内的数据发现菌体若产生大肠杆菌素或其毒性区域DNase domain时，会增加其对噬菌体的抵抗力，因此推测菌体所产生的大肠杆菌素除了对外的杀菌功能外，对内可能也具有抵抗噬菌体侵袭的生物功能。
Based on the in vitro and in vivo experimental data, we found that E. coli W3110 containing colicin or T2A-DNase domain can protect the cells against phage infection.
同时也经由突变进一步证实，ColE7 T2A-DNase domain的活性区域可能位於金属离子结合位置附近的Arg538及Glu542此两残基上，并由得来的所有结果推测大肠杆菌素、免疫蛋白质及其基质DNA之间相互作用的机转。
The stability of DNase-1 sensitive regions on chromosomes from normal lymphocytes, Burkitt's lymphoma cells, human embryonic. nasopharyngeal epithelial cells and lung fibroblasts was observed using in situ nick translation of chromosomes.
对正常人淋巴细胞，人胚鼻咽上皮细胞，人胚肺成纤维细胞，及Burkitt淋巴瘤(Burkitt`s lymphoma，BL)细胞染色体上的DNase—1敏感区的稳定性进行了观察。
At the same time, we found that PBCA-CTAB-DNA complexes could protect the DNA from degradation of DNaseⅠ, serum and sonication.
同时，实验也证实，PBCA纳米颗粒／DNA复合物能保护所携带的DNA免受核酸酶的降解和超声剪切作用。
Coli 5S ribosomal RNA, demonstrating there is no contamination of DNase or RNase in the cambialistic SOD preparation.
进一步研究发现被DNA拓扑异构酶I松弛的闭合双链DNA也能被SOD作用产生线状产物。
Objective To study the role of abnormal concentration of DNase in the development of SLE.
目的 研究血清DNA酶浓度异常在系统性红斑狼疮发生发展中所起的作用。
Covalent bond Crosslinking DNase Add antibody Isolate DNA Sequencing DNA Figure D.23 tation Chromatin immunoprecipi图 D.23 染色质免疫沉淀法 Instead of finding all of the DNA sequences to which a protein binds, other techniques can find all of the proteins to which a DNA sequence binds.
If 除了发现能与一种蛋白结合的所有 DNA 序列外，还有技术可以用来发现能与一种 DNA 序列结合的所有蛋白质。
Deoxyribonuclease II is an important acid endonuclease in lysosomes, nuclei and various secretions in many animals.
DNase II是核酸内切酶，在细胞内主要位于溶酶体、细胞核及各种分泌排泄物中，酸性条件(pH4.5–5.5)发挥最大活性。
The concentration of DNase was measured by single radial enzyme diffusion method in the sera of 63 SLE patients.
于国内首次用单相酶扩散法检测了63例SLE患者。
Methods The concentration of DNase was measured by single radial enzyme diffusion method in 63 serum samples of SLE patients.
于国内首次用单相酶扩散法检测了63例SLE患者。
Methods The concentration of DNase was measured by single radial enzyme diffusion method in the sera of 63 SLE patients.
于国内首次用单相酶扩散法检测了63例SLE患者。