The sediment core was collected from the shore on the Ardley Peninsula during the eighteenth China Antarctic Research Expedition using a 12cm-diameter PVC pipe. The number of cultural bacteria was higher than other areas of the Antarctic and there was an obvious decrease at 7th layer. The total DNA of every layer was extracted and the 16S rDNA was amplified by PCR. The diversity of 16S rDNA was studied by RFLP (Restriction Fragment Length Polymorphism) and phylogenetic analysis. In the sediment, bacteria belonging to the CFB (Cytophaga-Flexibacter-Bacterioides) group and the β-Proteobacteria, γ-Proteobacteria were the main groups. The 16S rDNA diversity of 7th layer was different from that of other layers, including the analysis of RFLP, gene type and the population of CFB group. And the population of β-Proteobacteria showed inverse ratio with that of CFB group. Fifteen percent of the total strains were related with degradation of hydrocarbon and one strain with heavy metal contamination.
本文作者于2001年11月至2002年4月参加了中国第18次南极考察队，合作采集了南极乔治王岛上阿德雷岛的企鹅粪便沉积物样品，分析了沉积物中可培养微生物，企鹅粪便沉积物中微生物数量比南极其他地区多，最多达到1×10~7个／g土样，并发现第7层次的可培养微生物数量突然降低；建立了提取南极沉积物的总DNA的方法，利用PCR-RFLP方法研究了沉积物中细菌的16S rDNA多样性，分析了沉积物中各类群细菌的数量和分布，发现沉积物中的细菌多样性较高，以CFB(Cytophaga-Flexibacter-Bacterioides)类群和β-、γ-Proteobacteria亚群的细菌为主，并且在第7层次处发现细菌的16S rDNA RFLP谱型数，基因型数和CFB类群细菌数量的异常，而β-Proteobacteria亚群和CFB类群细菌的数量呈反比关系；在沉积物中发现了大量的与降解有机污染物相关的细菌以及一株与重金属污染有关的细菌，在微生物生态方面找到了南极遭受有机物污染和重金属污染的证据；研究了沉积物各层次中各种氨基酸的种类与含量，发现沉积物中氨基酸种类和含量高于一般腐殖土，其中组氨酸、色氨酸、酪氨酸、甲硫氨酸和半胱氨酸在第2cm和第7cm处含量出现异常增多，其他氨基酸则在第2cm和第7cm处含量减少。
To study the morphological changes of Cytophaga hutchinsonii cell during its life circle.
研究哈氏噬纤维菌Cytophaga hutchinsonii 在生活史中细胞形态的变化。
The optimal temperature for growth and enzyme production was 27℃and 31℃pectively for Corynebacterium and both 32℃ for Cytophaga.
在500ml三角瓶中装50m1培养基，30℃，200r／min摇床上分别培养棒杆菌24h及噬纤维菌36h，每升发酵液分别产酶1700u和4000u。
Phylogentic analysis of Umbgl3A indicated umbgl3A may come from a species of Cytophaga.
遗传进化分析表明umbgl3A基因可是来自牛瘤胃微生物噬纤维菌属未培养的一个种。
After treated by ultraviolet and EMS, a mutant M2-4-4 was produced from Cytophaga MCA-2, with a higher enzyme activity of 66U/ml.
经过多次富集和反复筛选，从海洋环境中筛选到69株具有卡拉胶降解活性的菌株，以产酶活力高、生物学性状优良的MCA-2菌株为出发菌株，进行复合诱变和自然选育，获得了一株酶活显著提高的突变株M2-4-4，发酵液产酶活力达到66U/ml。
Results We discovered that under starvation conditions, the long, flexible rod cell of Cytophaga hutchinsonii would bend and turn into circular cell. The circular cell failed to produce carboxymethyl cellulase. Some of the circular cells might further wind around and turn into tiny spherical cells.
发现在饥饿状态下，长杆状菌体开始逐渐弯曲，菌体两端靠近成环形，环形菌体又进一步盘绕收缩成微小球形体，微小球形体在一定条件下能像生孢噬纤维菌的小孢囊一样萌发形成长杆状菌。
Using CMC plate screening method, a marine strain QM11 that can produce alkaline cellulase is isolated from the seawater of Qingdao coast. According to the 16S rDNA analysis, the strain belongs to the Cytophaga fucicola.
中文摘要：用CMC平板筛选方法，从青岛近海海域海水中分离出一株产碱性纤维素酶的海洋菌株QM11，经16S rDNA鉴定，该菌株为 Cytophaga fucicola 。
Sequencing of this 4.3kb DNA fragment revealed that there is a β-glucosidase gene urnbgl3A with an ORF of 1956bp, encoding product shared 63% identity and 79% similarity with a beta-glucosidase (GenBank NO:AAX16378.1) of uncultured murine large bowel bacterium BAC 31B, and shared 50% identity and 67% similarity with a beta-glucosidase-related glycosidases (GenBank NO:ZP-00308419.1) of Cytophaga hutchinsonii.
测序结果表明在4.3kb的片段上有一个全长为1956bp的ORF，编码一个可能的β-葡萄糖苷酶基因umbgl3A，其编码产物与一个来源於小鼠大肠未培养细菌的β-葡萄糖苷酶(GenBank Acession NO：AAX16378.1)一致性为63%、相似性为79%；与哈氏噬纤维菌的β-葡萄糖苷酶(GenBank Acession NO：ZP-00308419)的一致性为50%、相似性为67%。
The bacterial community composition was revealed by the constructed phylogenetic tree of known sequences and the sequences randomly selected from the libraries. We found that both samples contained low G+C Gram-positive bacteria and Cytophaga-Flexibacter-Bacteroides phyla as the majorities, and Spirochaetes as the minorities. LGCGPB accounts for 56.66% and 73.33% of the bacterial communities in buffalo rumen and its enriched culture.
结果 水牛瘤胃内容物与其富集培养物中均主要含有LGCGPB (low G+C Gram-Positive Bacteria)、CFB (Cytophaga-Flexibacter-Bacteroides)两大类菌群和少数的螺旋体菌，且LGCGPB所占的比例都是最高的，LGCGPB在水牛瘤胃内容物细菌中的比例为56.66%，而在富集培养物细菌中的比例升高为73.33%。
3, The effects of continuous cropping years on bacterial physiological groups were in the order: the total microbe number of ammoniation > nitrifier > azotobacter and cytophaga in investigated bacteria;
连作对微生物生理类群的影响，在分析的几类有益细菌生理群中，氨化细菌>硝化细菌>固氮菌和纤维素分解菌；
Evaluation of Cytophaga and Sporocytophaga as protein in feed
噬纤维菌和生孢噬纤维菌作为饲用蛋白质的综合评价
Most of the cellulases encoded by Cytophaga hutchinsonii contain neither carbohydrate-binding modules (CBMs) nor docking domain-like structures.
所编码的绝大部分纤维素酶类都不含有碳水化合物结合结构域（CBMs），也不含有类似于纤维小体中dockingdomain的结构域。
Cytophaga hutchinsonii could degrade quickly the filters and posses the efficient capability of cellulose degradation.
它能够迅速的降解滤纸，具有高效的纤维素降解能力。
Strain C 43, identified as Cytophaga.
初步鉴定C43菌株为噬纤维菌科、噬纤维菌属。
Studies on the serological type of Cytophaga columnaris
柱状嗜纤维菌血清型的研究
Cytophaga columnaris and Gill - rot Disease of Fish (Summary)
柱状噬纤维菌及鱼类烂鳃病（综述）