Based on the conserved region nucleotide sequences of four viruses (Alfalfa Mosaic Virus, ALMV; Cucumber mosaic virus, CMV; Cucumber mosaic virus-satellite, CMV-sat; Potato virus Y, PVY) and one viroid (Potato spindle tuber viroid, PSTVd) which could be pathogenic on potato world and one inner control(18S rRNA), specific oligonucleotide probes and primers were designed and spotting for hybridization detection.
利用芯片点样仪将5种侵染马铃薯的病毒/类病毒(苜蓿花叶病毒、黄瓜花叶病毒、黄瓜花叶病毒-卫星病毒、马铃薯病毒Y、马铃薯块茎纺锤状类病毒)的保守区寡核苷酸(Oligonucleotide,oligo)探针和PCR探针点样于玻片,并以植物18S rRNA作为内参照制成基因芯片。
Cucumber mosaic virus is one of the most economically important viruses occurring throughout the world.
黄瓜花叶病毒是世界范围内广泛分布的最具经济重要性的植物病毒之一。
Four kinds of virus particles with spherical, fibrous and short-rod shapes were found and the inclusion bodies of crystalloid, hexagon, bundle, scroll and pinwheel were observed in the cytoplasm of mesophyll cells by electron microscope. Enzyme-linked immumnosorbent assay indicated that the spherical and short fibrous viruses were cucumber mosaic virus(29.5 nm) and lily symptomless virus(593.29~639.97 nm×12.15~14.12 nm), while the long fibrous and short rod-shaped viruses were preliminarily identified as Potyvirus(757.58~846.25 nm×12.12~12.75 nm) and tobacco rattle virus(500~807.69 nm×153.85~192.31 nm).
通过带病组织汁液和带病组织超薄切片负染电镜技术对侵染观赏百合的病毒粒子和内含体进行观察,证明在表现不同症状的5个品种中存在球状、短线状、长线状、短杆状4种病毒粒子和晶体状、风轮状、束状、卷筒状、正六边形5种内含体,通过ELISA检测及病毒粒子形态、大小及内含体形态分析,确定球状病毒粒子(直径29.5 nm)为黄瓜花叶病毒,短线状病毒粒子(593.29~639.97 nm×12.15~14.12 nm)为百合无症病毒,长线状病毒粒子(757.58~846.25 nm×12.12~12.75 nm)为马铃薯Y病毒,短杆状病毒粒子(500~807.69 nm×153.85~192.31 nm)为烟草脆裂病毒。
The 2b protein encoded by Cucumber mosaic virus plays an important pathogenicity role in many solanaceous hosts, but mechanism of inducing disease is still unknown.
黄瓜花叶病毒(Cucumber mosaic virus,CMV)2b蛋白作为重要致病因子的角色在茄科模式植物上已为广泛描述,但其致病机理尚未廓清。
For the soybean germplasms, 76(25.33%), 41(13.67%) and 14 (4.67%) were positive to Soybean mosaic virus, Cucumber mosaic virus, and Alfalfa mosaic virus respectively.
结果表明:在大豆种质中检测出大豆花叶病毒、黄瓜花叶病毒、苜蓿花叶病毒三种病毒,阳性检出率分别为25.33%(76份)、13.67%(41份)和4.67%(14份)。
It reacted positively with cucumber mosaic virus (CMV) antiserum in the agar diffusion test.
经琼脂双扩散测定,TN分离物与黄瓜花叶病毒抗血清呈阳性反应。
Cucumber mosaic virus is one of most popular plant viruses in the world, and its host range is wide. It brings serious damages to agricultural production in pepper and is difficult to be controled.
黄瓜花叶病毒是世界上流行的植物病毒之一,其寄主范围广泛且较难防治,对农业生产造成严重危害。
Effects of post-acquisition fast on cucumber mosaic virus transmission by the cotton aphid, Aphis gossypii;
结果表明获毒后的禁食时间与棉蚜传毒效率呈负相关。
The simultaneous display technique of electrical penetration graph (EPG) was used to study the effects of the wave potential drop (pd) and its sub-phases in the acquisition of cucumber mosaic virus (CMV) by the cotton aphid Aphis gossypii from the infected melon plants.
利用刺吸电位(EPG)及其即时中断技术研究了棉蚜Aphisgossypii传播黄瓜花叶病毒(CMV)的机理,分析电势落差波(pd)及其亚波形在棉蚜获得CMV的取食行为过程中的作用。
A high-titre antiserum of Cucumber Mosaic Virus which titre was determined as 1/256 by double diffusion test was obtained by immunizing rabbits with purified CMV as antigen through intravenous, intramuscular, and footpad injection.
以提纯的CMV作为抗原,经静脉注射、肌肉注射和足垫注射等对家兔进行免疫,获得高效价的抗血清(琼脂双扩散效价为1/256);
Cloning and Sequence Analysis of Coat Protein Gene of Cucumber Mosaic Virus Isolate from Stripe and Necrosis Virus of Processing Tomatoes in Xinjiang A Poison Tree
新疆加工番茄条斑坏死病原黄瓜花叶病毒外壳蛋白基因的克隆和序列分析
Sequence Homology and Base Pairing between Cucumber Mosaic Virus Satellite RNA and Potato Spindle Tuber Viroid
黄瓜花叶病毒卫星RNA与马铃薯纺锤形块茎类病毒间序列同源性与碱基配对
Cloning and sequencing of a cucumber mosaic virus satellite RNA isolated from Raphanus sativus and comparison of its sequence with 12 satellite RNAs
黄瓜花叶病毒萝卜分离株卫星RNA的克隆及其与12个卫星RNA核苷酸序列的比较
Construction of plant expression vector of fusion genes with Banana bunchy top virus replicase and Cucumber mosaic virus coat protein
香蕉束顶病毒复制酶和黄瓜花叶病毒衣壳蛋白融合基因植物表达载体的构建
Cloning of GDD deleted replicase gene of Cucumber mosaic virus and construction of its plant expression vector
缺失GDD保守区的黄瓜花叶病毒复制酶基因的克隆及植物表达载体构建
Fast Homozygosity of Transgenic Tobacco Line Resistant to Cucumber Mosaic Virus
快速选育抗黄瓜花叶病毒的转基因烟草纯合品系
Resistance Identification and SSR Marking of Resistance Gene of Tobacco Cucumber Mosaic Virus
烟草CMV抗性鉴定及抗性基因的SSR标记研究
Inductive effect of methyl jasmonate on tobacco resistance to cucumber mosaic virus
茉莉酸甲酯对烟草抗黄瓜花叶病毒的诱导效应
Cucumber mosaic virus was detected from infected Piper nigrum Linn. in hainan with the indirect enzyme-linked immunosorbent assay.
通过间接酶联免疫法,从海南罹病胡椒植株中检测到黄瓜花叶病毒(Cucumber mosaic virus,CMV)。
The Analysis on the Characteristics of the Coat Protein Genes of Cucumber mosaic Virus Isolated from Echinacea purpurea Moench
黄瓜花叶病毒紫松果菊分离物外壳蛋白特性分析