The bacterial strain, MBM7070 F〓 lacZ (Am CA7070, lacY1 hsdR hsdM 7679 galH galk rpsc thi was transformed with Dpn1 treated plasmid DNA by the calcium chloride procedure and transformants were placed on Luria Broth plates containing ampicillin, X-gal (5-bromo-4-chloro-3-indoyl-β-D-galactoside) and IPTG (isopropyl-β-D-thiogalactoside).
然后用CaCl〓法将从细胞中提纯的质粒转化受体菌MBM7070F〓lacZ(AmCA70070，LacY1 hsdR hsdM7679 galH galK rpsc thi，将转化子细菌置于含氨苄青霉素、5-溴-4-氯-3-吲哚基-β-D-半乳糖苷(5-bromo-4-chloro-3-indoyl-β-D-galactoside，X-gal)以及异丙基-β-D-硫代半乳糖苷(isopropyl-β-D-thiogal-actoside，IPTG)的LB平板中。
The structures, functions and anticoagulant mechanisms of anticoagulation factors ACF I and ACF II from the venom of Agkistrodon acutus have been investigated. Both ACF I and ACF II form a 1:1 complex with activated coagulation factor X in a Calcium ion-dependent fashion which prevents the interaction of FXa with other proteins in the coagulation process, therefore ACF I and ACF II have marked anticoagulant activity.
中文摘要：对尖吻蝮蛇毒中抗凝血因子ACF I和ACF II的结构、功能和抗凝机理等进行了深入研究，发现ACF I和ACF II均与活化凝血因子X结合成1：1的复合物，从而阻止了FXa进一步参加凝血过程中反应，因而具有显著的抗凝血活性。
Using such means as chemical analysis, X-ray diffraction pattern, polarizing microscopy and scanning electronic microscopy, this paper has discussed the features and formation processes of secondary calcites in calcium nodules within ustic vertisols in Yuanmou Basin, Yunnan Province.
利用化学分析、X射线衍射、偏光显微镜和扫描电子显微镜等探讨云南省元谋盆地干润变性土钙质结核中的次生方解石的特征和形成过程。
It use X-RD diffraction to analyze content of coal ash which is useful to study infection of calcium oxide.
用X-RD衍射分析其物相组成，更好的探讨钙在煤灰熔点中所起的作用。
Using high performance liquid chromatography, X-ray diffraction and Fourier transformation infrared spectra, the authors characterized the sorted proteins from different otoliths. It is found that the three types of proteins in lapillus and asteriscus have different characteristics, and such differences may be responsible for different kinds of crystalline state in calcium carbonate.
通过高效液相色谱、X射线衍射、红外光谱分析和氨基酸成分分析等方法分别对3种基质蛋白进行了表征，发现微耳石和星耳石中3种基质蛋白特征存在差异，并初步推断蛋白质特征的差别与碳酸钙晶体在微耳石和星耳石中的不同晶型有关。
In this paper, with 2-oxo-propionic acid salicyloyl hydrazone as a ligand, one calcium complex, three copper complexes and thirteen rare earth(RE=La, Ce, Pr, Nd, Sm, Eu. Gd, Tb, Dy, Er, Tin, Yb and Lu) complexes have been synthesized. The crystals of four complexes were obtained, and the crystal structures were determined by single crystal X ray diffraction. One of the crystals is calcium complex, the other are binary and trinary complexes of copper.
本文选用2-羰基丙酸水杨酰腙作为配体，在水中分别与钙、铜、稀土(RE=La、Ce、Pr、Nd、Sm、Eu、Gd、Tb、Dy、Er、Tm、Yb和Lu)的盐反应，共制得了17种未见文献报道的配合物，另外还培养了2-羰基丙酸水杨酰腙、咪唑与铜、钙的二元及三元配合物的四种单晶，对La-Gd的稀土的配合物进行了溶解热的测定，并用Gauss-98软件包对铜的配合物进行了有关量化计算。
Some breast implants risks are associated particularly with implants such as capsular contracture, leak or rupture, change or loss of sensation, calcium deposits and interference with mammography readings.
一些乳房植入的风险是相关的，特别是与植入物，如包膜挛缩，渗漏或破裂，改变或损失的感觉，钙存款和干扰，与乳房X光造影检查读数。
By means of spin-coating technique, the thin films were deposited on Si(100)or glass substrates. The surface morphology evolution of the thin films on Si(100)substrate on annealing temperature were studied by SEM. X-ray diffraction patterns of CaMoO4 thin films prepared reveal that calcium molybdate grows in the solution by one step without any interim compounds, and the thin films are characteristic of oriented growth when the annealing temperature above 550℃.
通过旋涂技术将薄膜沉积在Si(100)或载玻片上，并利用SEM技术表征了薄膜的表面形貌，以及薄膜的表面随退火温度变化的特征。X射线衍射结果显示，在溶液中，钼酸钙化合物就已直接生成而不需经过任何中间过程。
The total radiative loss include the sum of every possible transition between two levels under the atomical models of hydrogen (4 level + 1 continuum), ionized calcium (5 level + 1 continuum), and magnesium (3 level) beside the contributions from nagtive hydrogen of f-f and f-b tranditions.
并将辐射损失速率与非热电子束轰击和软x线作用下的产能率作了比较，结果表明：耀斑不同时刻的色球辐射损失变化可达一个量级以上；耀斑脉冲相前后电子束加热相当有效；耀斑强度极大时，电子束轰击和软x线加热都有作用；耀斑主相时，软x线加热更能有效地平衡辐射损失。
Lysis solutions from imported and domestic genomic DNA purification kits, and 2% Trition X-100 and 5%guanidinium isothiocyanate were added into the oocysts, followed by different combining forms of freeze-thawing, proteinase K and sonication. Real-time PCR was used to determine the copies of Cryptosporidium oocyst wall protein gene. HCT-8 cells were infected by C. andersoni oocysts. Real-time PCR was used to determine the enhancement effect of fetal calf serum, glucose, ascorbic acid, folic acid, calcium pantothenate and insulin while added into RPMI-1640 medium.
在隐孢子虫卵囊悬液中分别加入进口试剂盒和国产试剂盒的裂解液以及2%Triton X-100和5%异硫氰酸胍，辅以不同组合方式使用的冻融法、蛋白酶K法和声裂法裂解卵囊，通过Real-time PCR法测定裂解产物中隐孢子虫卵囊囊壁蛋白(Cryptosporidium oocyst wall protein，COWP)基因拷贝数。
The construction characteristics of calcium phosphite were measured with Y-4Q system X-ray diffraction apparatus and the thermal analysis method in this paper.
本文采用热分析和x衍射方法研究了亚磷酸氢钙的结构特性，获得了亚磷酸氢钙结晶水合物的热分解特性和有关热分析数据；获得了亚磷酸氢钙结晶水合物的x衍射图谱和有关衍射数据；分析了它们与结构及其变化的关系。
The control and model groups were fed with 0.9% saline 10 ml/kg once a day. The Nlestriol group was fed with 1 mg/kg. The other three groups were fed with Gubaoye at the dose of 5, 10, 15 ml/kg once a day respectively. After fedding for 3 months, the concentration of calcium, tartrate resistant acid phosphatase in serumand alkaline phosphatase in bonewere measured by colorimetric method. The concentration of osteocalcin in serumwas measured by competitive radio immunologic method. Bone mineral density of femur and lumbar of rats was determined by Dual Engergy X-ray Absorbtiometry. Biomechanical characteristics of femur were measured by three point bending test. Histomorphometry of bone trabecula was analysed by scanning electron microscope.
正常对照组、模型组以0.9％生理盐水每天10ml／kg，尼尔雌醇组以尼尔雌醇每2周1mg／kg，其他三组每天分别以骨宝液5、10、15ml／kg的剂量灌胃给药3个月后，以比色法测定其血清钙、骨碱性磷酸酶和血清抗酒石酸酸性磷酸酶的活性，竞争放射免疫法测定血清中骨钙素的含量；双能x线骨密度仪测定股骨及腰椎的骨密度；三点弯曲试验法测定骨生物力学；扫描电镜进行骨小梁形态计量学分析。
It is indicated that the hexagonal calcium hydroferrite is a kind of high scattered calciferous compound. The interplanar spacing of hexagonal calcium hydroferrite is wide. The desilication activity of hexagonal calcium hydroferrite is higher. And the loss of alumina can be reduced.
结合X射线衍射分析讨论水合铁酸钙的脱硅机理，提出水合铁酸钙是一种高度分散的含钙化合物，其晶面间距较大，脱硅活性较高，并可大幅度降低铝损。
ZnO·xCaO·yP2O5 was synthesized by hydrothermal reaction of zinc oxide and calcium oxide with phosphoric acid for I ￣ 2h.
本文以ZnO，CaO和H3PO4为原料，利用水热法合成出一种新型三元化合物－ZnO·xCaO.yP2O5。其中，x＝0.2～0.6，y＝0.3～0.8（mo1）。XRD物相分析证明产品主要为无定形态。
The Scanning electron microscopy is used to study the morphological features of porcelaneous layer s on surface of freshwater cultured pearls from Zhuji, Zhejiang Province, and the calcium carbonate polymorph of porcelaneous layer s is identified by X-ray powder diffraction.
采用扫描电子显微镜对中国浙江诸暨三角帆蚌养殖珍珠表面特别是无珍珠光泽的瓷质层的微形貌进行了研究，并采用X射线粉晶衍射对瓷质层进行了物相鉴定。
The improved formula blends in well with drink and food, provides calcium and iron, and is ideal for health-conscious people.
每人每日所需的蛋白质可根据方程式体质(千克 x 0.75克计算，健尔力蛋白质粉可混和各式饮料、肉类、糕饼、硉瘚平驯峞A新配方更易与饮料及食物混和，质感口感亦更佳，加上奶蛋白浓缩素提供的钙质与铁质，更加营养丰富，是现代健康营养一族的必需品。
From this the crystalline field parameters of five transition metal ions in calcium silicate were determined half quantitatively, these transition metals are Cr, Mn, Fe, Co, Ni which have great effect on the color characteristics of decorative cement, their valence, coordination and replacing pattern were determined, too.
利用光学光谱，电子顺磁共振波谱，等离子发射光谱，X光电子能谱等现代测试手段系统地研究了第四周期过渡金属离子在硅酸三钙和硅酸二钙中的存在状态，及对硅酸钙颜色特性的影响。
In this paper, two kinds of porous calcium phosphate glass ceramics were sintered at 500℃ and 680℃ for 4 h respectives and degradation and biological compatibility properties were characterized. There are β-Ca(PO3)2 phases detected by X-ray diffraction in the latter glass ceramics (680℃ for 4 h).
分别在500、680℃下烧结4h后制备二种多孔钙磷玻璃陶瓷，并评价了它们的降解性能和生物相容性。X射线衍射证实，500℃下烧结产物呈玻璃态；而680℃下烧结产物有晶体析出，其主晶相为β-Ca(PO3)2。
As studied by X-ray energy spectrum and atomic absorption spectrum, the cell wall of Rhizopus Oryzae contains only 0.036% of calcium in dry weight.
用X－ray能谱及原子吸收光谱研究，发现Ca（Ⅱ）仅占米根霉细胞壁干重的0.036%。
Calcium magnesium acetate (CMA) is a green chemical. It can be used as deicer for highways and also as absorbent for NO x and SO 2 to control atmospheric pollution caused by coal combustion.
醋酸钙镁盐（CMA）是环保型化学品，可作为高速公路的除冰剂，也可作为NOx和SO2的吸收剂，控制煤燃烧造成的大气污染。