When reached logarithm growth phase, it was poured or the medium was removed. 5 mL medium containing 10 mg/L mitocin-C was added, kept at 37 ℃, and then cultured in saturated humidity warm box with the CO2 of 0.05 fraction volume for 2 or 3 hours. It was coated with 0.1% gelatin in 6-hole plate, laid for over 30 minutes, and then threw away or the medium with mitocin-C was removed, washed with phosptat buffer for 5 times so as to remove the mitocin-C. 2 mL 0.05% trypsin digestive cells were added, and it was observed under microscope. When crevice appeared, cells became round (about 2－4 minutes), digestion was stopped by adding medium of the same volume, and blew up with straws repetitively to make it into monoplast suspension. Special-used cover glass was put in the center of cell counting chamber. Cells were sucked in by glass siphon, and cell suspension flew out at bucket of up or down-sides of counting chamber, until the cover glass was filled with fluid. Living cell were inoculated at concentrations of 3×108, 5×108, 1×109 L－1 after their number counted.
选取2～5代的小鼠胚胎成纤维细胞，待其至对数生长期倒掉或吸掉培养基，加入含10 mg/L丝裂霉素C的细胞全培养液5 mL，置37 ℃，体积分数0.05的CO2饱和湿度温箱中培养二三小时，在6孔板中加入0.1%明胶包被，放置30 min以上，倒掉或吸掉含丝裂霉素C的培养基，磷酸盐缓冲液清洗5遍，尽量除去丝裂霉素C，加入2 mL 0.05%的胰蛋白酶消化细胞，镜下观察，当细胞间出现裂隙，细胞变圆时（约2～4 min），立即加入等量的全培养液终止消化，并用吸管反复吹打，使之成为单细胞悬液，在细胞计数板中央放置专用的盖玻片，用玻璃虹吸管吸取细胞，让虹吸管在盖玻片上或下侧的计数板凹槽处流出悬液，至盖玻片下被液体充满为止。
The results are as follows: compound (3) has no repellent against mosquito. On the other hand, it has been reported that compounds having structure simlar to 8-hydroxylcarveol have mosquito-repellent activity, such as p-menthendiol, etc. Perhaps the position of polar functional groups and chiral C atoms are important factors for mosquito-repellent activity. 8-Hydroxylcarveol propionate (6) has no mosquito-repellent activity mosquito, perhaps its carbonyl pars is too large. There is few report of propionate used as terpenoid repellent currently. 8 -Hydroxylcarveol acetate (5) has some repellent activity against mosquito, when its mass fraction is 20%, which can exhibit repelling time close to 4 h. 8-Hydroxylcarveol formate (4) has the longest repelling time of upto 7-8 h against mosquito, when its mass fraction is 20%, which is similar to the repellent activity of N, N-diethyl-m-toluamide.
结果显示：化合物3对白纹伊蚊没有驱避活性，与之结构及其类似的对烯二醇等已被以往的研究证明具有驱避活性，因此极性官能团和手性破原子位置可能是影响驱避活性的重要因素；衍生物6对白纹伊蚊没有驱避活性，这可能与其羰基部分太大有关，在目前的萜类驱避剂中尚极少见丙酸酯类；衍生物5具有一定的驱避活性，质量分数为20%时，对白纹伊蚊的驱避时间接近4h；衍生物4的驱避效果最好，质量分数为20%时，对白纹伊蚊的驱避时间可以达到7~8h，与避蚊胺的驱避效果相同。
RESULTS:Six flavonoids 6-C-glycopyranosyl-8-C-glycopyranosyl apigenin(1), 6-C-arabinosyl-8-C-glycopyranosyl apigenin(2), 6-C-glycopyranosyl-8-C-arabinosyl apigenin(3), 6-C-glycopyranosyl-8-C-xyloeyl apigenin(4), 6-C-glycopyranosyl luteolin(5), luteolin(6), one chromone isobiflorin(7), and one lignanoid-isolariciresinol(8), were isolated from the n-BuOH fraction of this plant, respectively.
结果：从毛鸡骨草地上部分的正丁醇部位分离并鉴定了8个化合物，黄酮类化合物：芹菜素-6-C-葡萄糖-8-C-葡萄糖苷（1），芹菜素-6-C-阿拉伯糖-8-C-葡萄糖苷（2），芹菜素-6-C-葡萄糖-8-C-阿拉伯糖苷（3），芹菜素-6-C-葡萄糖-8-C-木糖苷（4），木犀草素-6-C-葡萄糖苷（5），木犀草素（6）；色原酮类化合物：异双花母草素（7）；木脂素类化合物：-异落叶松树脂醇（8）。
The C 5 Fraction derived from ethylene steamcracker contains some very useful components, isoprene, cyclopentadiene and piperylene.
来自裂解乙烯的 C5馏份含有非常有用的化工原料——异戊二烯、环戊二烯、戊间二烯。
A piperylene based petroleum resin was modified by the monoolefin fraction from C_5 separation unit.
采用 C5分离装置得到的 C5单烯烃馏分对间戊二烯石油树脂进行改性。
Research on Precipitation and Decomposition Process of Zinc Carbonate for Preparing Activated Zinc Oxide Catalyst for Selective Hydrogenation of C_5 Fraction from Steam Cracking
碳酸锌的沉淀及分解制备活性氧化锌裂解碳五馏分选择加氢催化剂的研究
Preparation of tertiary amyl alcohol from hydration of isoamylene enriched C 5 fraction was carried in the laboratory. The effects of catalyst, reaction temperature, pressure, space velocity and water/isoamylene ratio were investigated.
在实验室研究了从富含异戊烯的C5馏分水合制备叔戊醇的工艺，考察了催化剂、反应温度、压力、空速、水与异戊烯摩尔比等因素对水合反应的影响。
Synthesis of methyl tert - amyl ether from c_5 fraction
由碳五馏分合成甲基叔戊基醚工艺的研究
Preparation of tertiary amyl alcohol from iso amyl ene enriched c_5 fraction
富含异戊烯的C5馏分合成叔戊醇的研究
C 5 of FCC gasoline should be the preferential fraction as etherification feed with the promise that the production specification must be meet.
指出在满足醚化要求的前提下，应优先选择C5馏分做醚化原料；
Proximate Analysis of Cracking C 5 Fraction by GC FTIR and GC MS Methods Study on the determination of NO、AsH_3、COS and other impurity in pyrolysis gas by chromatography/mass spectrometry method
GC/FTIR法和GC/MS法联合分析裂解碳五馏份的组成色质联用分析裂解气中NO、AsH3、COS等杂质
Catalyst for Selective Hydrogenation of C_5 Fraction from Steam Cracking
裂解碳五馏分选择加氢催化剂的研究
Catalytic reaction principles and catalysts for complete conversion of c_5 fraction into isopentene ⅱ. production of isopentene based on Dehydrogenation of isopentane
C5全组分异构烯烃化的催化反应原理与催化剂Ⅱ.异戊烷脱氢制异戊烯
Development and Utilization of Isoprene from C_5 Cracking Fraction
裂解C5馏分中异戊二烯的开发和利用
One-stage extraction technology of separating polymer-grade isoprene from cracked C_5 fraction
从裂解碳五分离聚合级异戊二烯的一段萃取工艺
Discussion on the utilization of c_5 fraction obtained from steam cracking
对我国裂解碳五馏分分离和利用的探讨
Separation of cyclopentadiene from C_5 cut fraction by thermal dimerization
热二聚法分离C5馏分中的环戊二烯
Analysis of Cracking C_5 Fraction and Reaction Kinetics of Dienes/Maleic Anhydride; Macro-kinetics study on bubbling carbonation of Li_2CO_3 slurries at atmospheric pressure
裂解碳五常微量分析及双烯/顺酐反应动力学碳酸锂常压鼓泡碳化宏观反应动力学研究
Study on thermal dimerization of dienes in C_ 5 fraction
裂解C5馏分中二烯烃热二聚过程的研究
Reaction behaviors of C_5 dienes in thermal dimerization were investigated by the seal-tube method. Reaction temperature, residence time and different cyclopentadiene (CPD) content in C_5 fraction were taken into account. Results indicated that, CPD conversion would be greater than 92%;
用封管实验方法考察了二聚反应温度、反应停留时间和不同环戊二烯（CPD）含量对C5馏分热二聚过程中各二烯烃组分反应行为的影响。