A significant difference was observed between group HG and group CHG at the first and the second turn of the basilar membrane.
噪声暴露后毛细胞中F-actin、CaM及HSP70的表达均呈增加趋势，各实验组三种蛋白的表达变化趋势基本相同。
Results we found positive labeling for both anp and nos in different types of cells including the spiral modiolar artery, stria vascularis, spiral limbus, spiral ligaments, basilar membrane, organ of corti, spiral ganglion, and macula sacculi. however, no immunoactivity was found in the reissner membrane and tectorial membrane.
结果 在耳蜗各转螺旋动脉和血管纹，螺旋缘、螺旋韧带和corti器显示双阳性染色，螺旋神经节细胞及囊斑神经上皮细胞膜及轴突nos阳性染色，胞质anp阳性染色；盖膜、前庭膜阴性染色。
Methods 20 guinea pigs were randomly divided into the experimental group and control group. By perfusing the antibody to GLAST into tympanic canal in the cochlea of guinea pigs in experimental group and artificial perilymph into the guinea's cochlea in control group, the results of ABR, basilar membrane stretched preparation and transmission electron microscope were observed.
健康豚鼠20只随机分为实验组和对照纸，每组10只实验组于蜗鼓阶内灌注GLAST抗体，对照组灌注入工外淋巴液，观察两组术后3、6、9ABR反应阈、耳蜗基底膜铺片和透射电镜的形态学改变。
Results After immunofluorescence and histochemistry staining, the overall intergrity of the tissue section could be preserved, including basilar membrane, tectona1 membrane and Reissner's membrane, hair cell and supporting cells. The nuclei were very distinct.
结果 经免疫荧光和组织化学染色后组织切片形态结构完整，耳蜗基底膜平直，盖膜及前庭膜完整，毛细胞与支持细胞间结构完整，细胞核清晰。
Results①Make clear of the general principal during the morphologicaldevelopment of the C57BL/6 mouse inner ear and reconstructed the 3D modelof E13 inner ear; ②The mature time of many histological structuresassociated closely with the hearing setout of C57BL/6 mouse were madeclear as basilar membrane in D10, scala vestibular and tympanicD4, Corti's tunnel D12, inner sulcus D10, tectorial membrane D10, haircells D12, spiral ganglion neurons D12 and neurofilament in cochlear axisD12-D14.③Outlined the time table and the patterns of the expressionsof those proteins associated with the inner ear development which includedproneural committed proteins, neuronal differential proteins, neurotrophic factors and neuron special proteins.
结果①HE常规染色大体观上了解了C57BL／6小鼠内耳形态发育E8-Da期间的一般规律并且获得了E13的三维立体图像；②获得了各种听功能密切相关的结构的成熟时间点，基底膜(D10)、鼓阶前庭阶(D4)、螺旋器隧道(D12)、内螺旋沟(D10)、盖膜(D10)、毛细胞(D12)、螺旋神经元(D12)和蜗轴纤维(D12-D14)；③完成了前神经决定蛋白、神经分化蛋白、神经营养因子和神经元特异性蛋白等内耳发育相关的蛋白的表达时限表并详细观察了其在内耳各个部位表达随发育变化的特点：④Calbindin在E16以后的螺旋神经元没有表达，但是在scarpa神经元持续表达。
The distribution of AQP1 consisted of cells lining the bony labyrinth, fibrocytes lining the endolymphatic duct and sac, cells under the basilar membrane, fibrocytes of the spiral ligament and the spiral limbus, Cortirs organ, inner and outer spiral sulcus, stria vascularis, saccular and utricular wall, and spiral ganglion.
AQP3、7、8的分布类似，在螺旋神经节和包绕膜迷路的组织中均有表达，其中Corti器、内外螺旋沟、血管纹、螺旋神经节表达较强，在螺旋韧带、螺旋缘纤维细胞表达较弱。
In order to study the prective effect of P27 in the process of toxic deafness of aminogiycosides, the P27 was examined in the cochlear by immunohistochemistry. The results showed that in the normal group P27 was extremely expressed in the cell plasma and nuclear of spiral ganglion, the ratio of positive cell displayed by buffy bead was 50%, then the supporting cell on the basilar. membrane was secondary, the dyeing of plasma and nuclear was obviously, the spiral ligand and the stria vascularis was weak in plasma and very weak in nuclear.
为了研究P27分子在氨基糖甙类抗生素中毒性耳聋时，对内耳各组分细胞是否有保护作用，我们用免疫组化法检测了P27在氨基糖甙类抗生素中毒性耳聋大鼠耳蜗的表达，结果表明在正常组大鼠耳蜗，P27在螺旋神经节表达最显著，在细胞核和细胞浆均有表达，阳性细胞约为50％，表现为胞浆和胞核的棕黄色颗粒，基底膜上的支持细胞表达强度次之，在细胞浆和细胞核均有明显染色，螺旋韧带和血管纹主要为细胞浆较弱染色，细胞核染色极弱。
The most sensitive P27-mediated cell cycle regulating route was on the supporting of basilar membrane, spiral ganglion stria vasculiaria, but insensitive on the hair cell.. The weaked expression of P27 in the toxic deafness group showed that P27 mediated the proliferation and apoptosis of supporting cell to promte the proliferation and preclude the apoptosis so as that the hair cell could be repaired. P27 which was not expressed in the hair cell would not take part in the repairment of hair cell directly, however, P27 promoted the restore of hair cell by the regulation of supporting cell which was profitable for the recovery of the basilar membrane and the function compensation of the neighboring hair cell, then P27 is a beneficial factor to the hearing restore.
由此我们认为，P27在正常大鼠耳蜗除毛细胞外的其它结构中表达，表明P27介导的细胞周期调节途径在基底膜支持细胞、螺旋神经节和血管纹中各组分细胞中可能敏感，而在毛细胞细胞周期进行中的调节则不敏感；P27在庆大霉素中毒性耳聋大鼠耳蜗阳性表达部位的表达减弱，表明P27可通过调节基底膜支持细胞的增殖与调亡，促进支持细胞增殖阻断调亡，有利于支持细胞对毛细胞损伤部位的修复；耳蜗基底膜毛细胞不表达P27，在药物中毒性耳聋大鼠P27不直接参与毛细胞增殖与调亡的调节。
The expressional pattern in the deafness group was as above, but there was the weakened dyeing, especially on the supporting cell. So we realized that P27 was expressed in the structure except the hair cell. The most sensitive P27-mediated cell cycle regulating route was on the supporting of basilar membrane, spiral ganglion stria vasculiaria, but insensitive on the hair cell.. The weaked expression of P27 in the toxic deafness group showed that P27 mediated the proliferation and apoptosis of supporting cell to promte the proliferation and preclude the apoptosis so as that the hair cell could be repaired.
由此我们认为，P27在正常大鼠耳蜗除毛细胞外的其它结构中表达，表明P27介导的细胞周期调节途径在基底膜支持细胞、螺旋神经节和血管纹中各组分细胞中可能敏感，而在毛细胞细胞周期进行中的调节则不敏感；P27在庆大霉素中毒性耳聋大鼠耳蜗阳性表达部位的表达减弱，表明P27可通过调节基底膜支持细胞的增殖与调亡，促进支持细胞增殖阻断调亡，有利于支持细胞对毛细胞损伤部位的修复；耳蜗基底膜毛细胞不表达P27，在药物中毒性耳聋大鼠P27不直接参与毛细胞增殖与调亡的调节。
Along the membrane, the cavernous sinus can be reached. After drilling the posterior clinoid process, exposing the dorsum sellae and the upper clivus, the superior segment of the basilar artery can be exposed.
结果 磨除前床突后形成的间隙为床突间隙；前床突下颈内动脉与动眼神经间的膜为颈内动脉动眼神经膜，沿此膜可进入海绵窦；磨除后床突后，暴露鞍背、上斜坡，即可显露基底动脉上段。
Results With orbitozygomatic approach, drilling the anterior clinoid process got a space called the clinoid space. The membrane between the internal carotid artery beneath the anterior clinoid process and the oculomotor nerve is called carotidoculomotor membrane. Along the membrane, the cavernous sinus can be reached. After drilling the posterior clinoid process, exposing the dorsum sellae and the upper clivus, the superior segment of the basilar artery can be exposed.
结果 磨除前床突后形成的间隙为床突间隙；前床突下颈内动脉与动眼神经间的膜为颈内动脉动眼神经膜，沿此膜可进入海绵窦；磨除后床突后，暴露鞍背、上斜坡，即可显露基底动脉上段。
In this method, we intend to imitate the frequency analysis function of the basilar membrane, the phase-locking function of the afferent auditory nerve and the feed back function of the efferent auditory nerve during the auditory process.
在这种方法中，我们试图模仿人耳基底膜的频率分析功能、上行听觉神经系统的相位锁定功能和下行听觉神经系统的反馈功能，用自适应滤波器组代替传统的固定带宽的滤波器组，提取特征参数。
Methods A small hole with a diameter about 0.4mm, which had the distance of 2.4mm from round window niche, was made on cochlear basic turn of guinea pig for measuring the velocity of basilar membrane in vivo.
方法在豚鼠耳蜗底回距圆窗龛缘24mm处开一直径约04mm小孔，作为测量活体基底膜的振动速度测试窗。
VEGF is mainly located in the inflammatory cells and epithelial cells surrounding the basilar membrane and inflammatory cells and endothelial cells surrounding the vessels.
VEGF在鼻息肉组织中主要定位于基底膜周围的炎性细胞和上皮细胞以及腺体、血管周围和血管壁内皮细胞。
The basilar membrane once had the report bronchial tube asthma patient bronchial tube basilar membrane accumulation.
基底膜曾有报道支气管哮喘患者支气管基底膜增厚。
Result: GEPRB can reduce the cumulating thickness of the basilar membrane and that of the vascular pattern, so as to tighten up the sparsity of the spiral ganglion cells.
结果：豁痰祛瘀颗粒能降低糖尿病大鼠升高的血糖，减轻糖尿病大鼠早期耳蜗基底膜、血管纹增厚程度，使稀疏的螺旋神经节细胞变致密。
Sensory hair cells with overlymg tectorial membrane could be identified in the basilar papilla.
基底乳头在内淋巴腔面有感觉毛细胞，其表面可见盖膜。
To explore the relationship between hearing damage and hair cell loss, we studied the relationship of cortical hearing threshold with hair cell loss rate in an unit of length of the basilar membrane in guinea pigs.
为了探讨听觉损伤与毛细胞损伤的关系，本实验比较了噪声暴露后豚鼠皮层听阈及其与基底膜单位长度上毛细胞损伤率的关系。
Results We found positive labeling for both ANP and NOS in different types of cells including the spiral modiolar artery (SMA), stria vascularis, spiral limbus, spiral ligaments, basilar membrane, organ of Corti, spiral ganglion, and macula sacculi.
结果在耳蜗各转螺旋动脉和血管纹，螺旋缘、螺旋韧带和Corti器显示双阳性染色，螺旋神经节细胞及囊斑神经上皮细胞膜及轴突NOS阳性染色，胞质ANP阳性染色；
Results The velocity of basilar membrane vibration elicited with tone was increased significantly when positive current was delivered to scala vestibuli and the velocity was decreased on the contrary.
结果当外加电流前庭阶极性为正，鼓阶极性为负时，可以看到基底膜振动速度显著增大，给相反极性电流时，基底膜振动速度减小。